Paraffin section immunohistochemical staining box

Abstract

The utility model discloses a paraffin section immunohistochemical staining box, which belongs to the technical field of staining boxes and is characterized by comprising a staining box body, the left side of the staining box body is fixedly connected with a preheating mechanism, the left side of the preheating mechanism is fixedly connected with a temperature control mechanism, and the preheating mechanism heats a placing plate through a heating plate. The reagent in the placing groove is further preheated, so that the reagent is at a proper temperature when being in contact with the paraffin section, the binding reaction of the reagent and a target antigen is accelerated, the time required for dyeing is shortened, and the dyeing efficiency is effectively improved; the temperature in the placing groove is detected in real time by a detection head of the temperature control mechanism through a temperature measuring hole; according to the preheating device, the temperature sensor is arranged on the heating plate and transmits a temperature signal to the temperature sensor, the temperature sensor transmits the signal to the microcontroller, and the microcontroller accurately controls the heating power of the heating plate according to a preset temperature value, so that accurate regulation and control of the preheating temperature are realized.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of dyeing box, especially to a paraffin section immunohistochemical dyeing box. BACKGROUND

[0002] In the field of modern medicine and biology research, paraffin section immunohistochemical dyeing technology is a very important and widely used research method, which can locate, qualify and relatively quantitatively analyze specific proteins in tissue cells through the specific binding reaction between antigen and antibody, and provides key basis for disease diagnosis, pathological mechanism research and drug development.

[0003] For the above problems, the existing patent gives a solution, but the existing dyeing box lacks a structure for preheating reagents, which leads to that in the immunohistochemical dyeing process, when the reagent contacts the paraffin section, the temperature difference is large, so that the reagent cannot quickly and fully combine with the target antigen, thereby reducing the dyeing efficiency and the stability of the dyeing effect.

[0004] Therefore, a paraffin section immunohistochemical dyeing box is proposed. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a paraffin section immunohistochemical dyeing box, which can solve the problem that the existing dyeing box lacks a structure for preheating reagents, which leads to that in the immunohistochemical dyeing process, when the reagent contacts the paraffin section, the temperature difference is large, so that the reagent cannot quickly and fully combine with the target antigen, thereby reducing the dyeing efficiency and the stability of the dyeing effect.

[0006] To achieve the above object, the utility model provides the following technical scheme: a paraffin section immunohistochemical dyeing box, comprising a dyeing box body, a preheating mechanism fixedly connected to the left side of the dyeing box body, and a temperature control mechanism fixedly connected to the left side of the preheating mechanism.

[0007] The preheating mechanism comprises a limiting box, a placing plate, three placing grooves and three heating plates, the limiting box is fixedly connected to the left side of the dyeing box body, the placing plate is fixedly connected to the inner side of the limiting box, the placing grooves are opened in the top of the placing plate, and the heating plates are fixedly connected to the bottom of the placing plate.

[0008] Preferably, the temperature control mechanism comprises temperature measuring holes, detection heads, temperature sensors and microcontrollers, and the temperature measuring holes are respectively opened in the left side of the placing groove and the left side of the limiting box.

[0009] Preferably, the detection end of the detection head is fixedly connected to the inner side of the temperature measuring hole, and the temperature sensor is fixedly connected to the end away from the temperature measuring hole of the detection head.

[0010] Preferably, the microcontroller is fixedly connected to the front side of the limiting box, and a surface of the microcontroller is coated with heat insulation paint.

[0011] Preferably, the placing plate is made of aluminum alloy material, the bottom of the placing groove is fixedly connected with a protective pad, and the protective pad is made of aluminum nitride ceramic material.

[0012] Preferably, the surface of the heating plate is fixedly connected with a connecting rod, and a surface of the connecting rod is coated with high-temperature-resistant ceramic paint.

[0013] Preferably, the top of the limiting box is clamped with a cover plate, the bottom of the cover plate is fixedly connected with a heat insulation plate, and the heat insulation plate is made of polyurethane foam material.

[0014] Preferably, the top of the cover plate is fixedly connected with a handle, the handle is sleeved with a protective sleeve, and a surface of the protective sleeve is engraved with anti-skid lines.

[0015] Compared with the prior art, the utility model has the advantages that:

[0016] 1. The preheating mechanism preheats the reagent placed in the placing groove through the heating plate, so that the reagent is at an appropriate temperature when contacting the paraffin section, accelerates the binding reaction of the reagent and the target antigen, shortens the time required for dyeing, and effectively improves the dyeing efficiency.

[0017] 2. The temperature control mechanism detects the temperature in the placing groove through the temperature measuring hole in real time, transmits the temperature signal to the temperature sensor, transmits the signal to the microcontroller through the temperature sensor, and accurately controls the heating power of the heating plate according to the preset temperature value, so as to realize accurate control of the preheating temperature. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a whole structure diagram of the paraffin section immunohistochemical dyeing box of the utility model;

[0019] Figure 2 It is a whole structure diagram of the preheating mechanism of the utility model;

[0020] Figure 3 It is a structure schematic view of the microcontroller of the utility model;

[0021] Figure 4 It is a whole structure diagram of the paraffin section immunohistochemical dyeing box of the utility model Figure 3 It is an enlarged schematic view of A in the utility model;

[0022] Figure 5 It is a structure schematic view of the heat insulation plate of the utility model.

[0023] In the figure, 1, dyeing box body; 2, preheating mechanism; 21, limiting box; 22, placing plate; 23, placing groove; 24, heating plate; 3, temperature control mechanism; 31, temperature measuring hole; 32, detection head; 33, temperature sensor; 34, microcontroller; 4, protective pad; 5, connecting rod; 6, cover plate; 7, heat insulation plate; 8, handle; 9, protective sleeve. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0025] Please refer to Figures 1-5 The utility model provides technical schemes:

[0026] A paraffin section immunohistochemical dyeing box, including dyeing box body 1, preheating mechanism 2 is fixedly connected to the left side of dyeing box body 1, and temperature control mechanism 3 is fixedly connected to the left side of preheating mechanism 2;

[0027] Preheating mechanism 2 includes limiting box 21, placing plate 22, three placing grooves 23 and three heating plates 24, limiting box 21 is fixedly connected to the left side of dyeing box body 1, placing plate 22 is fixedly connected to the inner side of limiting box 21, placing groove 23 is opened in the top of placing plate 22, and heating plate 24 is fixedly connected to the bottom of placing plate 22.

[0028] In the embodiment: by setting dyeing box body 1 is the basic structure of storing and operating immunohistochemical dyeing related reagent and tool, provides the carrier for preheating mechanism 2 and temperature control mechanism 3 and realizes the function of paraffin section immunohistochemical dyeing, limiting box 21 can support and limit placing plate 22 and temperature control mechanism 3, placing plate 22 can quickly and evenly transmit the heat generated by heating plate 24 to placing groove 23, placing groove 23 can simultaneously accommodate three reagents to preheat, improve work efficiency, can satisfy the demand of batch dyeing, reduce the time cost of single section preheating, and different placing grooves 23 can place different processing or different reagents respectively, facilitate operator to carry out contrast experiment or multiple sample detection, improve the practicability and flexibility of dyeing box, and heating plate 24 provides heat source for preheating process, and by three heating plates 24, the bottom of placing groove 23 can be heated more evenly, so that the reagent in placing groove 23 is heated more evenly.

[0029] Specifically, as Figure 3 , Figure 4As shown, the temperature control mechanism 3 comprises a temperature measuring hole 31, a detection head 32, a temperature sensor 33 and a microcontroller 34. The temperature measuring hole 31 is respectively arranged on the left side of the placement groove 23 and the left side of the limiting box 21.

[0030] Specifically, as shown in Figure 3 , Figure 4 The detection end of the detection head 32 is fixedly connected to the inner side of the temperature measuring hole 31, and the temperature sensor 33 is fixedly connected to the end of the detection head 32 away from the temperature measuring hole 31.

[0031] Specifically, as shown in Figure 3 , Figure 4 The microcontroller 34 is fixedly connected to the front side of the limiting box 21, and the surface of the microcontroller 34 is coated with heat insulation paint.

[0032] In this embodiment: the temperature measuring hole 31 provides an installation position for the detection head 32, which can accurately measure the temperature in the placement groove 23. The detection head 32 can sense the temperature change in the placement groove 23 and can transmit the temperature signal to the temperature sensor 33 in real time, ensuring the accuracy and timeliness of the temperature data. The temperature sensor 33 converts the temperature signal from the detection head 32 into an electrical signal that can be recognized and processed by the microcontroller 34, accurately transmits the temperature information to the microcontroller 34. The microcontroller 34 receives the temperature signal from the temperature sensor 33 and compares it with the preset temperature value, and controls the working state of the heating plate 24 according to the comparison result, such as adjusting the heating power or turning on / off the heating plate 24, to realize the control of the preheating temperature.

[0033] Specifically, as shown in Figure 2 The placement plate 22 is made of aluminum alloy material, and the bottom of the placement groove 23 is fixedly connected with a protective pad 4 made of aluminum nitride ceramic material.

[0034] Specifically, as shown in Figure 3 The surface of the heating plate 24 is fixedly connected with a connecting rod 5, and the surface of the connecting rod 5 is coated with high-temperature resistant ceramic paint.

[0035] In the embodiment, the placing plate 22 is made of aluminum alloy material, which has good heat conduction performance and high thermal conductivity, so that the heat generated by the heating plate 24 can be quickly and uniformly conducted to the placing groove 23. The protective pad 4 is made of aluminum nitride ceramic material, which has excellent heat insulation performance and can reduce the heat loss at the bottom of the placing groove 23, thereby helping to maintain the stability of the temperature in the groove, ensuring the preheating effect of the paraffin section. The connecting rod 5 provides a fixed and supported structure for the heating plate 24, so that it can be stably installed at the bottom of the placing plate 22, ensuring that the heating plate 24 will not shift or loosen during operation, and ensuring that the heat can be stably transferred to the placing plate 22, thereby effectively preheating the reagent. The high-temperature-resistant ceramic coating can effectively protect the connecting rod 5 from being damaged in a high-temperature environment, prolonging the service life of the connecting rod 5.

[0036] Specifically, as shown in Figure 5 The top of the limiting box 21 is clamped with a cover plate 6, and the bottom of the cover plate 6 is fixedly connected with a heat insulation plate 7 made of polyurethane foam material.

[0037] Specifically, as shown in Figure 5 The top of the cover plate 6 is fixedly connected with a handle 8, and the handle 8 is sleeved with a protective sleeve 9 having anti-slip lines on the surface.

[0038] In the embodiment, the cover plate 6 is provided to facilitate the operation and maintenance of the preheating mechanism 2 inside. When the reagent needs to be placed or taken out, the user can easily open the cover plate 6 and tightly close it after operation, effectively preventing dust, impurities and the like from entering the limiting box 21. The heat insulation plate 7 can prevent the surface temperature of the cover plate 6 from being too high, avoiding burns to the operator during operation and improving the safety of use. The heat insulation plate 7 is made of polyurethane foam material, which has excellent heat insulation performance and low thermal conductivity, effectively blocking the heat loss inside the limiting box 21, maintaining the stability of the preheating temperature, and reducing energy consumption. The handle 8 is provided to facilitate the operator to open and close the cover plate 6, making the operation more labor-saving and convenient. The protective sleeve 9 can provide a more comfortable grip for the operator, avoiding hand fatigue caused by long-time operation. The anti-slip lines increase the friction between the hands and the handle 8, making the operator hold the handle 8 more firmly.

[0039] Working principle: first, the user will dye box body 1 to the designated work position and placed, then, the user switch on the microcontroller 34 power and start the device, microcontroller 34 immediately issue instructions, control temperature sensor 33 and detection head 32 start work, real-time temperature detection of the placement slot 23 inside, when the detection head 32 sense the temperature of the placement slot 23 is lower than the preset appropriate temperature, will immediately transmit the corresponding temperature signal to temperature sensor 33, temperature sensor 33 receives the signal, quickly temperature data processing and transmission to microcontroller 34, microcontroller 34 based on the received data analysis and judgment, once confirmed that the temperature is too low, will immediately control three heating plate 24 start working, heating plate 24 start to the space in the placement slot 23 heating, make the temperature gradually back to the appropriate range, when ready to carry out antigen antibody detection, the user start dye box body 1 of the relevant operation procedures, the detection of the paraffin section to be placed in the dye box body 1 inside the designated position, then, the user from the refrigeration equipment required for this detection reagent, at this time, the user holds the handle 8 on the top of the cover plate 6, through the force cover plate 6 and limiting box 21 separation, then, the user will take out the reagent into the placement slot 23 one by one, after the reagent placed, the user again cover plate 6 and limiting box 21 clamping, make the preheating mechanism 2 re in the closed state, at this time, the preheating mechanism 2 in the placement slot 23 generated by the hot air and placed in the reagent full contact, realize the reagent heating operation, make the reagent to reach the temperature conditions suitable for paraffin section reaction, finally, when the reagent complete heating, reach the ideal reaction temperature, the user again through the handle 8 cover plate 6 and limiting box 21 separation, the user takes out the reagent has been heated, and add it to the dye box body 1, make the reagent and previously placed good paraffin section full contact and specific binding reaction, so as to complete the subsequent immunohistochemical staining detection process.

[0040] The above is only the preferred embodiment of the present application, and does not limit the present application, any modification, equivalent replacement and improvement within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A paraffin section immunohistochemical staining kit, comprising a staining kit body (1), characterized in that: A preheating mechanism (2) is fixedly connected to the left side of the dyeing box body (1), and a temperature control mechanism (3) is fixedly connected to the left side of the preheating mechanism (2). The preheating mechanism (2) includes a limiting box (21), a placement plate (22), three placement slots (23) and three heating plates (24). The limiting box (21) is fixedly connected to the left side of the dyeing box body (1). The placement plate (22) is fixedly connected to the inside of the limiting box (21). The placement slots (23) are opened on the top of the placement plate (22). The heating plates (24) are fixedly connected to the bottom of the placement plate (22).

2. The paraffin section immunohistochemical staining kit according to claim 1, characterized in that: The temperature control mechanism (3) includes a temperature measuring hole (31), a detection head (32), a temperature sensor (33), and a microcontroller (34). The temperature measuring hole (31) is respectively opened on the left side of the placement slot (23) and the left side of the limiting box (21).

3. The paraffin section immunohistochemical staining kit according to claim 2, characterized in that: The detection end of the detection head (32) is fixedly connected to the inside of the temperature measuring hole (31), and the temperature sensor (33) is fixedly connected to the end of the detection head (32) away from the temperature measuring hole (31).

4. The paraffin section immunohistochemical staining kit according to claim 2, characterized in that: The microcontroller (34) is fixedly connected to the front side of the limiting box (21), and the surface of the microcontroller (34) is coated with heat-insulating paint.

5. The paraffin section immunohistochemical staining kit according to claim 1, characterized in that: The placement plate (22) is made of aluminum alloy, and a protective pad (4) is fixedly connected to the bottom of the placement groove (23). The protective pad (4) is made of aluminum nitride ceramic material.

6. The paraffin section immunohistochemical staining kit according to claim 1, characterized in that: A connecting rod (5) is fixedly connected to the surface of the heating plate (24), and the surface of the connecting rod (5) is coated with a high-temperature resistant ceramic coating.

7. The paraffin section immunohistochemical staining kit according to claim 1, characterized in that: The top of the limiting box (21) is snapped with a cover plate (6), and the bottom of the cover plate (6) is fixedly connected with a heat insulation plate (7), which is made of polyurethane foam material.

8. The paraffin section immunohistochemical staining kit according to claim 7, characterized in that: The top of the cover plate (6) is fixedly connected to a handle (8), and the handle (8) is covered with a protective sleeve (9), the surface of which is engraved with anti-slip texture.

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