Improved high-throughput protein antibody purification instrument

Abstract

The utility model relates to the field of antibody purification, in particular to an improved high-flux protein antibody purification instrument which comprises a mounting frame, a plurality of heat preservation bins are arranged on the mounting frame, a purification column is arranged in each heat preservation bin, a snakelike bent pipe wound on the outer side of the purification column is further arranged in each heat preservation bin, and the snakelike bent pipes are connected with the heat preservation bins. A feeding pipe and a discharging pipe are further arranged on the mounting frame, the feeding pipe is detachably connected with one end of the purification column, and the discharging pipe is detachably connected with the other end of the purification column. The antibody purification instrument is simple and convenient to operate, realizes simultaneous purification of multiple groups of antibodies, realizes rapid installation and disassembly of the purification column, and can adjust the purification temperature of the antibodies, so that the antibodies can be purified under the optimal temperature condition, and the antibody purification effect is further improved; a cooling or heating medium is introduced into the snake-shaped bent pipe, so that the antibody in the purification column is at the optimal temperature.

Description

Technical Field

[0001] This utility model relates to the field of antibody purification, specifically an improved high-throughput protein antibody purification instrument. Background Technology

[0002] Antibody purification is a crucial step in biopharmaceutical and research processes, aiming to isolate high-purity antibodies from complex mixtures such as serum, cell culture supernatants, or tissue extracts. High-quality antibodies are essential for diagnostic reagents, therapeutics, and basic research.

[0003] Existing high-throughput protein antibody purification instruments typically utilize multiphase peristaltic pumps to simultaneously supply liquid to multiple tubing lines, allowing for the purification of multiple antibodies through multiple purification columns, thereby improving purification efficiency. However, during use, the inconvenience of adjusting the temperature of the purification columns prevents antibodies from being purified under optimal temperature conditions, thus affecting the antibody purification effect and reducing the practicality of the device. Summary of the Invention

[0004] To overcome the shortcomings of the prior art, this utility model provides an improved high-throughput protein antibody purification instrument, which enables the simultaneous purification of multiple groups of antibodies, allows for rapid installation and disassembly of the purification column, and allows for adjustment of the antibody purification temperature, enabling the antibody to be purified under optimal temperature conditions, thereby improving the antibody purification effect.

[0005] This invention provides an improved high-throughput protein antibody purification instrument, including a mounting frame with multiple insulated chambers. Each insulated chamber contains a purification column, and each chamber also contains a serpentine bend wound around the outside of the purification column. The mounting frame also includes an inlet pipe and an outlet pipe. The inlet pipe is detachably connected to one end of the purification column, and the outlet pipe is detachably connected to the other end. The two ends of the purification column are connected to the inlet and outlet pipes, respectively. The inlet pipe is connected to a peristaltic pump, which supplies liquid to multiple pipelines, allowing for the simultaneous purification of multiple antibody groups and improving purification efficiency. During purification, a cooling or heating medium is circulated through the serpentine bend to maintain the antibody in the purification column at an optimal temperature, ensuring the purification efficiency of the device.

[0006] As a further technical solution, an inlet ring is fixed to the top of the insulation chamber, and the inner sidewall of the top of the inlet ring is inclined. When the purification column is inserted into the insulation chamber, the insertion direction of the purification column can be adjusted and centered by the inclined surface.

[0007] As a further technical solution, a heat-conducting limiting ring is provided inside the insulation chamber. The inner diameter of the heat-conducting limiting ring is adapted to the outer diameter of the purification column, and the serpentine bend is sleeved on the outside of the heat-conducting limiting ring. By setting the heat-conducting limiting ring, on the one hand, the temperature of the serpentine bend can be quickly transferred to the purification column, and on the other hand, the purification column can be limited to prevent it from tilting during installation and can be better connected with the discharge pipe.

[0008] As a further technical solution, adjacent serpentine bends are connected end to end by a connecting pipe.

[0009] As a further technical solution, the inlet end of the front-end serpentine bend is connected to a liquid inlet pipe, and the outlet end of the rear-end serpentine bend is connected to a liquid outlet pipe. By connecting the two ends of the serpentine bends with connecting pipes, the heating / cooling medium can be introduced into multiple serpentine bends from the liquid inlet pipe and discharged from the liquid outlet pipe.

[0010] As a further technical solution, the front end of the feed pipe is connected to several L-shaped corrugated pipes corresponding to the purification column, and the ends of the L-shaped corrugated pipes are fixed with connecting rings.

[0011] As a further technical solution, the end of the connecting ring is rotatably provided with a feed connector via a bearing, and the feed connector is threadedly connected to one end of the purification column. The bellows is set to L-shape to facilitate the insertion of the purification column into the insulation chamber. After connecting the bottom end of the purification column to the discharge pipe, the feed connector is pulled to the top of the purification column and screwed onto the top of the purification column to connect the feed connector to the top of the purification column.

[0012] As a further technical solution, the discharge pipe is connected to several discharge connectors corresponding to the purification column, and the discharge connectors are threadedly connected to the other end of the purification column. During installation, the purification column is inserted from the top of the insulation chamber and screwed into the discharge connector, thus achieving a quick connection between the bottom end of the purification column and the discharge pipe.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0014] 1. This antibody purification instrument is simple and convenient to operate, enabling the simultaneous purification of multiple groups of antibodies. It also allows for the rapid installation and disassembly of the purification column and the adjustment of the antibody purification temperature, ensuring that the antibody is purified under optimal temperature conditions, thereby improving the antibody purification effect.

[0015] 2. This antibody purification instrument is combined with a serpentine tube, which is wound around the outside of the purification column. During the purification process, a cooling or heating medium is introduced into the serpentine tube to keep the antibody in the purification column at the optimal temperature, thus ensuring the purification efficiency of the device.

[0016] 3. This antibody purification instrument uses an inlet ring and a thermally conductive limiting ring to quickly position the purification column, allowing both ends of the purification column to be quickly connected to the outlet and inlet connectors, respectively, thus improving the practicality of the device. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the accompanying drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the structure of an improved high-throughput protein antibody purification instrument provided in an embodiment of the present invention.

[0019] Figure 2 This is a schematic diagram of an improved high-throughput protein antibody purification instrument provided in an embodiment of the present invention, without the installation of a purification column.

[0020] Figure 3 This is a schematic diagram showing the positions of the serpentine bend and the purification column in the incubation chamber of an improved high-throughput protein antibody purification instrument provided in this embodiment of the present invention.

[0021] Figure 4 This is a schematic diagram of a purification column for an improved high-throughput protein antibody purification instrument provided in an embodiment of the present invention.

[0022] Figure 5 This is a schematic diagram of the connection structure between the feed connector and the purification column of an improved high-throughput protein antibody purification instrument provided in this embodiment of the present invention.

[0023] In the diagram: 1. Mounting frame; 2. Insulation chamber; 3. Purification column; 4. Serpentine bend; 5. Feed pipe; 6. Discharge pipe; 7. Inlet ring; 8. Thermal limiting ring; 9. Connecting pipe; 10. Liquid inlet pipe; 11. Liquid outlet pipe; 12. L-shaped corrugated pipe; 13. Connecting ring; 14. Feed connector; 15. Discharge connector. Detailed Implementation

[0024] The terms “comprising” and “having”, and any variations thereof, in the specification, claims, and accompanying drawings of this utility model are intended to cover a non-exclusive inclusion, such as a process, method, system, product, or device that includes a series of steps or units, not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or devices.

[0025] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model. In addition, the technical features of the various embodiments or individual embodiments provided by this utility model can be arbitrarily combined to form new technical solutions. Such combinations are not bound by the order of steps and / or structural composition patterns, but must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0026] Please see Figure 1-5 This utility model provides an improved high-throughput protein antibody purification instrument, including a mounting frame 1. The mounting frame 1 is provided with multiple insulated chambers 2, each insulated chamber 2 is provided with a purification column 3, and the insulated chamber 2 is also provided with a serpentine bend 4 wrapped around the outside of the purification column 3. The mounting frame 1 is also provided with an inlet pipe 5 and an outlet pipe 6. The inlet pipe 5 is detachably connected to one end of the purification column 3, and the outlet pipe 6 is detachably connected to the other end of the purification column 3.

[0027] The two ends of the purification column 3 are connected to the feed pipe 5 and the discharge pipe 6, respectively. The feed pipe 5 is connected to the peristaltic pump, which enables the supply of liquid to multiple pipelines and the simultaneous purification of multiple groups of antibodies, thereby improving the purification efficiency. During the purification process, a cooling or heating medium is introduced into the serpentine bend 4 to keep the antibody in the purification column 3 at the optimal temperature, thus ensuring the purification efficiency of the device.

[0028] It should be noted that temperature sensing elements (not shown in the figure) can be installed at the inlet, middle and outlet of purification column 3 to monitor the temperature distribution inside the column in real time.

[0029] In this embodiment, an inlet ring 7 is fixed to the top of the heat preservation chamber 2, and the inner sidewall of the top of the inlet ring 7 is inclined.

[0030] By setting an inlet ring 7 with a sloping inner wall at the top of the heat preservation chamber 2, the purification column 3 can be easily inserted into the heat preservation chamber 2. When the purification column 3 is inserted into the heat preservation chamber 2, the insertion direction of the purification column 3 can be adjusted and centered by the action of the sloping surface.

[0031] In this embodiment, a heat-conducting limiting ring 8 is provided inside the heat-insulating chamber 2. The inner diameter of the heat-conducting limiting ring 8 is adapted to the outer diameter of the purification column 3, and the serpentine bend 4 is sleeved on the outside of the heat-conducting limiting ring 8.

[0032] By setting the heat-conducting limiting ring 8, on the one hand, the temperature of the serpentine bend 4 can be quickly transferred to the purification column 3, and on the other hand, the purification column 3 can be limited so that it will not tilt during installation and can be better connected with the discharge pipe 6.

[0033] In this embodiment, adjacent serpentine bends 4 are connected end to end by connecting pipes 9. The inlet end of the front serpentine bend 4 is connected to a liquid inlet pipe 10, and the outlet end of the rear serpentine bend 4 is connected to a liquid outlet pipe 11.

[0034] By connecting the two ends of the serpentine bends 4 through the connecting pipe 9, the heating / cooling medium can be introduced into the multiple serpentine bends 4 from the inlet pipe 10 and then discharged through the outlet pipe 11.

[0035] In this embodiment, the front end of the feed pipe 5 is connected to a plurality of L-shaped corrugated pipes 12 corresponding to the purification column 3. The end of the L-shaped corrugated pipe 12 is fixed with a connecting ring 13. The end of the connecting ring 13 is rotatably provided with a feed connector 14 through a bearing. The feed connector 14 is threadedly connected to one end of the purification column 3.

[0036] When installing the purification column 3, it needs to be inserted from the top of the insulation chamber 2. Therefore, the feed pipe 5 and the discharge pipe 6 are not on the same vertical plane. The feed pipe 5 is located behind the top of the discharge pipe 6. At the same time, the corrugated pipe is set to L-shape to facilitate the insertion of the purification column 3 into the insulation chamber 2. After connecting the bottom end of the purification column 3 to the discharge pipe 6, the feed connector 14 is pulled to the top of the purification column 3 and screwed onto the top of the purification column 3 to connect the feed connector 14 to the top of the purification column 3.

[0037] In this embodiment, the discharge pipe 6 is connected to a plurality of discharge connectors 15 corresponding to the purification column 3, and the discharge connectors 15 are threadedly connected to the other end of the purification column 3.

[0038] During installation, insert the purification column 3 from the top of the insulation chamber 2 and screw it into the discharge connector 15 to achieve a quick connection between the bottom of the purification column 3 and the discharge pipe 6.

[0039] In summary, in use, the purification column 3 is first inserted through the inlet ring 7 at the top of the insulation chamber 2, passing through the insulation chamber 2 and screwing its bottom end into the outlet connector 15. Then, the inlet connector 14 is pulled to the top of the purification column 3 and screwed onto the top of the purification column 3, connecting the inlet connector 14 to the top of the purification column 3. After the installation of the purification column 3 is completed, the peristaltic pump is used to supply liquid to multiple pipelines, purifying multiple groups of antibodies simultaneously and improving purification efficiency. During the purification process, cooling or heating media is introduced into the serpentine bend 4 to keep the antibodies in the purification column 3 at the optimal temperature, ensuring the purification efficiency of the device.

[0040] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the technical solutions of the embodiments of this utility model.

Claims

1. An improved high-throughput protein antibody purification instrument, characterized in that, The device includes a mounting frame (1), which is provided with multiple heat preservation chambers (2). Each heat preservation chamber (2) is provided with a purification column (3). The heat preservation chamber (2) is also provided with a serpentine bend (4) wrapped around the outside of the purification column (3). The mounting frame (1) is also provided with a feed pipe (5) and a discharge pipe (6). The feed pipe (5) is detachably connected to one end of the purification column (3), and the discharge pipe (6) is detachably connected to the other end of the purification column (3).

2. The improved high-throughput protein antibody purification instrument according to claim 1, characterized in that, The top of the heat preservation chamber (2) is fixed with an inlet ring (7), and the inner side wall of the top of the inlet ring (7) is a slope.

3. The improved high-throughput protein antibody purification instrument according to claim 1, characterized in that, The heat-conducting limiting ring (8) is provided inside the heat-conducting limiting ring (8). The inner diameter of the heat-conducting limiting ring (8) is adapted to the outer diameter of the purification column (3). The serpentine bend (4) is sleeved on the outside of the heat-conducting limiting ring (8).

4. The improved high-throughput protein antibody purification instrument according to claim 1, characterized in that, The adjacent serpentine bends (4) are connected end to end by a connecting pipe (9).

5. The improved high-throughput protein antibody purification instrument according to claim 4, characterized in that, The inlet end of the front serpentine bend (4) is connected to an inlet pipe (10), and the outlet end of the rear serpentine bend (4) is connected to an outlet pipe (11).

6. The improved high-throughput protein antibody purification instrument according to claim 1, characterized in that, The front end of the feed pipe (5) is connected to a number of L-shaped corrugated pipes (12) corresponding to the purification column (3), and the end of the L-shaped corrugated pipe (12) is fixed with a connecting ring (13).

7. The improved high-throughput protein antibody purification instrument according to claim 6, characterized in that, The end of the connecting ring (13) is provided with a feed connector (14) through a bearing, and the feed connector (14) is threadedly connected to one end of the purification column (3).

8. The improved high-throughput protein antibody purification instrument according to claim 7, characterized in that, The discharge pipe (6) is connected to a number of discharge connectors (15) corresponding to the purification column (3), and the discharge connectors (15) are threadedly connected to the other end of the purification column (3).

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