Eluting device used in coating reaction of anti-coating / non-coating tube

Abstract

The utility model relates to the technical field of diagnostic reagents, in particular to an elution device used in a coating reaction for placing a coating-free tube, which comprises a support frame and a support seat, a rotating shaft is arranged on the support seat, one end of the rotating shaft is connected with a driving device, the other end of the rotating shaft is provided with an object placing disc with a hollow structure, and a plurality of placing grooves are arranged on the object placing disc. A liquid storage disc is arranged between every two adjacent containing grooves. A plurality of liquid adding pipes are arranged above the storage tray, and each liquid adding pipe is communicated with the washing liquid conveying pipe through a conveying pump; a plurality of washing liquid extraction branch pipes are arranged above one side of the storage disc, one end of each washing liquid extraction branch pipe is communicated with a waste washing liquid storage tank through a washing liquid extraction main pipe, the waste washing liquid storage tank is communicated with a buffer tank through a pipeline, and the buffer tank is communicated with an electric suction device; the liquid adding pipe is provided with an electromagnetic valve and a flow meter, and the electromagnetic valve, the flow meter and the conveying pump are electrically connected with a controller. According to the elution device, the washing liquid can be added and extracted without manual operation of personnel.

Description

Technical Field

[0001] This utility model relates to the field of diagnostic reagent technology, and in particular to an elution device used in radioimmunoassay coating reaction. Background Technology

[0002] Radioimmunoassay (RIA) tube coating is an application of radioimmunoassay. Its basic principle is to immobilize antibodies on the surface of a solid-phase immunosorbent assay (IPA) tube (usually a plastic tube or microplate), and then add the sample and labeled antigen or antibody. Through competitive binding, unlabeled antigens or antibodies bind to the antibodies immobilized on the tube surface, while labeled antigens or antibodies bind to the remaining antibodies. By measuring the signal from the radionuclide, the content of antigen or antibody in the sample can be calculated. However, the following problems exist in actual operation: After the sample and labeled antigen are added to the coated tube and incubated, the operator usually adds washing solution from a wash bottle to the inside of the coated tube for washing, and then uses a syringe to aspirate the washing solution, repeating this process twice. Currently, a liquid pump is used to deliver the washing solution into the coated tube. Although this reduces the labor intensity to some extent, it still requires the operator to hold the washing solution delivery tube and align it with the coated tube. After the washing solution is added, the operator needs to hold the coated tube and shake it back and forth to ensure thorough washing. In addition, residual washing solution in the delivery tube may spill after the washing solution is added. Therefore, to address the above problems, it is necessary to develop an elution device that eliminates the need for coating reaction in coated tubes. Utility Model Content

[0003] The technical problem to be solved by this utility model is to provide an elution device for coating reaction of coating tubes, which automatically adds and extracts the washing liquid without the need for manual operation by the staff, thus greatly improving work efficiency.

[0004] To solve the above-mentioned technical problems, the technical solution of this utility model is as follows:

[0005] An elution apparatus for coating reactions of coating tubes includes a support frame with a movably mounted support base. A rotating shaft is mounted on the support base, one end of which is connected to a drive device. The other end of the rotating shaft has a hollow placement tray with multiple placement slots adapted to the coating tubes. A storage tray is located between adjacent placement slots. Multiple liquid addition pipes are located above the placement tray, each connected to a washing liquid delivery pipe via a delivery pump. Multiple height-adjustable washing liquid extraction branch pipes, corresponding to the placement slots, are located above one side of the placement tray. One end of each washing liquid extraction branch pipe is connected to a waste washing liquid storage tank via a main washing liquid extraction pipe. The waste washing liquid storage tank is connected to a buffer tank via a pipe, and the buffer tank is connected to an electric suction device. A solenoid valve and a flow meter are mounted on the liquid addition pipes, and the solenoid valve, flow meter, and delivery pump are electrically connected to a controller.

[0006] As an improved technical solution, the support base is slidably mounted on the support plate of the support frame.

[0007] As an improved technical solution, the support frame is provided with a first fixing plate and a second fixing plate, and the first fixing plate and the second fixing plate are respectively provided with a first support plate and a second support plate. A plurality of liquid adding pipes are respectively fixed on the first support plate; a plurality of washing liquid extraction branch pipes are fixed on the second support plate, and the second support plate is slidably connected to the second fixing plate.

[0008] As an improved technical solution, lead plate protective covers are respectively provided on the outer wall of the waste washing liquid storage tank and the outer wall of the buffer tank.

[0009] As an improved technical solution, each of the washing liquid extraction branches is provided with a washing liquid suction head at its extraction end.

[0010] After adopting the above technical solution, the beneficial effects of this utility model are:

[0011] The elution device used in the coating reaction of the coating tube includes a support frame, a movable support base on the support frame, a rotating shaft on the support base, one end of the rotating shaft connected to a drive device, and the other end of the rotating shaft having a hollow placement tray. The placement tray has multiple placement slots adapted to the coating tubes, and a liquid storage tray is placed between two adjacent placement slots. Above the placement tray are multiple liquid addition pipes, each of which is connected to a washing liquid delivery pipe via a delivery pump. Above one side of the placement tray are multiple height-adjustable washing liquid extraction branch pipes corresponding to the placement slots. One end of each washing liquid extraction branch pipe is connected to a waste washing liquid storage tank via a main washing liquid extraction pipe. The waste washing liquid storage tank is connected to a buffer tank via a pipe, and the buffer tank is connected to an electric suction device. The liquid addition pipes are equipped with a solenoid valve and a flow meter, and the solenoid valve, flow meter, and delivery pump are electrically connected to a controller. In practical applications, after the incubation reaction is completed, the coated tubes are placed into the placement tank. The controller starts the delivery pump, solenoid valve, and flow meter, allowing the washing solution to enter the interior of the coated tubes. When the set flow rate is reached, the solenoid valve and flow meter close. The drive device drives the rotating shaft, placement tray, and multiple coated tubes to rotate. The washing solution dripping from the liquid addition tube is stored in the storage tray. The drive device drives the placement tray to rotate, causing the washing solution to agitate, thereby achieving thorough washing of the interior of the coated tubes. Then, the support base moves to one side, and the coated tubes in the placement tray move to below the washing solution extraction branch pipe as the motor rotates. The washing solution extraction branch pipe moves downward, so that the extraction end of the washing solution extraction branch pipe is located inside the coated tube. Under the action of the electric suction device, the washing solution in each coated tube is drawn into the waste washing solution storage tank, and a buffer tank prevents the waste washing solution from entering the electric suction device. After elution is completed, the washing solution extraction branch pipe moves upward again, and the coated tubes are removed for measurement and analysis. The above-mentioned elution device is reasonably designed and easy to operate. It can add and extract the washing solution without the need for manual operation.

[0012] The support base is slidably mounted on the support plate of the support frame. This design allows the support base to move left and right along the support frame.

[0013] The support frame is equipped with a first fixed plate and a second fixed plate, and the first fixed plate and the second fixed plate are respectively equipped with a first support plate and a second support plate. Multiple liquid addition pipes are fixed on the first support plate; multiple washing liquid extraction branch pipes are fixed on the second support plate, and the second support plate is slidably connected to the second fixed plate. This design achieves the fixation of the liquid addition pipes and also allows for the adjustment of the height of the washing liquid extraction branch pipes.

[0014] Because each dispensing tube has a tapered end, this design prevents spillage when adding the cleaning solution.

[0015] Lead plates are installed on the outer walls of both the waste washing liquid storage tank and the buffer tank. These lead plates effectively prevent radiation exposure to workers.

[0016] Each washing solution extraction branch is equipped with a washing solution suction tip at its extraction end. The design of the suction tip facilitates the extraction of the washing solution. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the elution device used in the coating reaction of a coating tube according to the present invention;

[0018] Among them, 1-support frame, 2-support base, 3-rotating shaft, 4-placement tray, 40-placement trough, 5-coating tube, 6-storage tray, 7-liquid filling pipe, 70-solenoid valve, 71-flow meter, 8-transfer pump, 9-washing liquid transfer pipe, 10-washing liquid extraction branch pipe, 11-washing liquid extraction main pipe, 12-waste washing liquid storage tank, 13-pipeline, 14-buffer tank, 15-electric suction device, 16-controller, 17-lead plate protective cover, 18-washing liquid suction head, 19-drive device, 190-motor, 191-gear, 20-first fixing plate, 21-second fixing plate, 22-first support plate, 23-second support plate, 24-fixing ring. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0020] An elution device for coating reactions without coating tubes, such as Figure 1 As shown, the device includes a support frame 1, a support base 2 movably mounted on the support frame 1, and a rotating shaft 3 mounted on the support base 2 (with a bearing between the support base and the rotating shaft). One end of the rotating shaft 3 is connected to a drive device (including a motor, with the motor's output shaft and the rotating shaft connected via gear transmission). The other end of the rotating shaft 3 is provided with a hollow storage tray 4, which has multiple placement slots 40 adapted to the coated tube 5. A liquid storage tray 6 is provided between two adjacent placement slots 40. Multiple liquid inlet pipes 7 are provided above the storage tray 4. Pipe 7 is connected to washing liquid delivery pipe 9 via delivery pump 8; multiple height-adjustable washing liquid extraction branch pipes 10 corresponding to the placement tank 40 are provided on one side of the placement tray 4, one end of each washing liquid extraction branch pipe 10 is connected to waste washing liquid storage tank 12 via washing liquid extraction main pipe 11, waste washing liquid storage tank 12 is connected to buffer tank 14 via pipe 13, buffer tank 14 is connected to electric suction device 15; solenoid valve 70 and flow meter 71 are provided on liquid addition pipe 7, solenoid valve 70, flow meter 71 and delivery pump 8 are electrically connected to controller 16 respectively.

[0021] In practical applications, after the incubation reaction is completed, the coated tubes are placed into the placement tank. The controller starts the delivery pump, solenoid valve, and flow meter, allowing the washing solution to enter the interior of the coated tubes. When the set flow rate is reached, the solenoid valve and flow meter close. The drive device drives the rotating shaft, placement tray, and multiple coated tubes to rotate. The washing solution dripping from the liquid addition tube is stored in the storage tray. The drive device drives the placement tray to rotate, causing the washing solution to agitate, thereby achieving thorough washing of the interior of the coated tubes. Then, the support base moves to one side, and the coated tubes in the placement tray move to below the washing solution extraction branch pipe as the motor rotates. The washing solution extraction branch pipe moves downward, so that the extraction end of the washing solution extraction branch pipe is located inside the coated tube. Under the action of the electric suction device, the washing solution in each coated tube is drawn into the waste washing solution storage tank, and a buffer tank prevents the waste washing solution from entering the electric suction device. After elution is completed, the washing solution extraction branch pipe moves upward, and the coated tubes are then removed for measurement and analysis.

[0022] The support base 2 is slidably mounted on the support plate of the support frame 1. Specifically, a slide rail and slider assembly is provided between the support base and the support plate of the support frame. The slide rail of the slide rail and slider assembly is fixed to the support plate, and the support base and the slider of the slide rail and slider assembly are fixedly connected. An electric cylinder is also included, with its output shaft fixedly connected to the slider. The electric cylinder is electrically connected to a controller (this part is a common structure and is not shown in the attached diagram). In practical applications, the controller activates the electric cylinder, causing the support base to move left and right along the support plate on the support frame.

[0023] The support frame 1 is equipped with a first fixing plate 20 and a second fixing plate 21. The first fixing plate 20 and the second fixing plate 21 are respectively equipped with a first support plate 22 and a second support plate 23. Multiple liquid addition pipes 7 are fixed to the first support plate 20 by fixing rings. Multiple washing liquid extraction branch pipes 11 are fixed to the second support plate 23 by fixing rings, and the second support plate 23 is slidably connected to the second fixing plate 21 (specifically, a slide rail slider assembly is provided between the second support plate and the second fixing plate; the slide rail of the slide rail slider assembly is fixed to the second fixing plate; the second support plate is fixedly connected to the slider of the slide rail slider assembly; an electric cylinder is also included, with its output shaft fixedly connected to the slider, and the electric cylinder is electrically connected to a controller; this is a common structure and is not shown in the attached diagram). This design achieves the fixing of the liquid addition pipes and also allows for the adjustment of the height of the washing liquid extraction branch pipes.

[0024] Lead plate protective covers 17 are installed on the outer walls of the waste washing liquid storage tank 12 and the buffer tank 14, respectively. By designing lead plate protective covers, the radiation of radioactive elements to the workers' bodies can be effectively prevented.

[0025] Each of the washing solution extraction branches 10 is equipped with a washing solution suction head 18 at its extraction end. The design of the washing solution suction head facilitates the extraction of washing solution.

[0026] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An elution apparatus for use in coating reactions of uncoated tubes, characterized in that, The device includes a support frame, on which a support base is movably mounted. A rotating shaft is mounted on the support base, with one end connected to a drive device and the other end equipped with a hollow placement tray. The placement tray has multiple slots adapted to the coated tubes, and a liquid storage tray is positioned between adjacent slots. Above the placement tray are multiple liquid addition pipes, each connected to a washing liquid delivery pipe via a delivery pump. Above one side of the placement tray are multiple height-adjustable washing liquid extraction branch pipes corresponding to the placement slots. One end of each branch pipe is connected to a waste washing liquid storage tank via a main washing liquid extraction pipe. The waste washing liquid storage tank is connected to a buffer tank via a pipe, and the buffer tank is connected to an electric suction device. The liquid addition pipes are equipped with a solenoid valve and a flow meter, and the solenoid valve, flow meter, and delivery pump are electrically connected to a controller.

2. The elution apparatus for use in a coating reaction of a radioactive coating tube according to claim 1, characterized in that, The support base is slidably mounted on the support plate of the support frame.

3. The elution apparatus for use in a coating reaction of a radioactive coating tube according to claim 1, characterized in that, The support frame is provided with a first fixing plate and a second fixing plate. The first fixing plate and the second fixing plate are respectively provided with a first support plate and a second support plate. A plurality of liquid addition pipes are respectively fixed on the first support plate. A plurality of washing liquid extraction branch pipes are fixed on the second support plate, and the second support plate is slidably connected to the second fixing plate.

4. The elution apparatus for use in a coating reaction of a radioactive coating tube according to claim 1, characterized in that, The end of each of the liquid inlet tubes is tapered.

5. The elution apparatus for use in a coating reaction of a radioactive coating tube according to claim 1, characterized in that, Lead plate protective covers are respectively installed on the outer wall of the waste washing liquid storage tank and the outer wall of the buffer tank.

6. The elution apparatus for use in a coating reaction of a radioactive coating tube according to claim 1, characterized in that, Each of the aforementioned washing solution extraction branches is equipped with a washing solution suction head at its extraction end.

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