Tissue protein and nucleic acid extraction sleeve for zebrafish embryos and juvenile fishes

Abstract

The utility model discloses a zebrafish embryo and juvenile tissue protein and nucleic acid extraction sleeve which comprises an outer sleeve and an inner sleeve, the inner sleeve is installed on the top of the outer sleeve through a neck tube, and the neck tube comprises a short neck tube and a long neck tube. The inner sleeve is sequentially matched with the short neck pipe and the long neck pipe to be installed in the outer sleeve, the whole of the inner sleeve, the short neck pipe and the outer sleeve is used in cooperation with a centrifugal machine, fish culture water / fry culture liquid is removed, and zebrafish embryos / juvenile fishes without water are obtained at the bottom of the inner sleeve; then the whole body of the inner sleeve, the long-neck tube and the outer sleeve is matched with an automatic grinding instrument for use, so that the zebrafish embryos / juvenile fishes in the inner sleeve extend into the nucleic acid / protein extraction solution and are fully ground and extracted; the whole of the inner sleeve, the short-neck tube and the outer sleeve is used in cooperation with a centrifugal machine, the broken zebrafish embryo / juvenile fish tissue in the inner sleeve is converged to the inner bottom of the outer sleeve, a nucleic acid / protein mixed solution is obtained, the outer sleeve is centrifuged, and supernatant is taken, so that the nucleic acid / protein solution can be obtained.

Description

TECHNICAL FIELD

[0001] The utility model relates to tissue protein / nucleic acid extraction cannula technical field, concretely is a zebra fish embryo, fry's tissue protein, nucleic acid extraction cannula. BACKGROUND

[0002] Zebra fish embryo / fry is very small in size, and the swimming speed is fast, needs to collect zebra fish embryo / fry to carry out extraction DNA, RNA or protein and other related experiments, usually first uses the suction tube to suck the fish into the collection pipe, then utilizes the suction tube or pipettor to remove the water in the collection pipe slowly in the mode of sucking, in this process, the small fish swims fast in it, and it is very easy to suck away the fish, and repeatedly sucking in and blowing out can also easily harm the zebra fish embryo / fry and even cause death, this method not only is time-consuming and laborious, but also can have certain influence to the quality of sample, and then increase the error of experimental result, therefore the utility model provides a zebra fish embryo, fry's tissue protein, nucleic acid extraction cannula to solve such problems. SUMMARY

[0003] The utility model discloses a zebra fish embryo, fry's tissue protein, nucleic acid extraction cannula to solve the problem in the background art.

[0004] In order to achieve the above object, the utility model provides the following technical scheme: a zebra fish embryo, fry's tissue protein, nucleic acid extraction cannula, including outer sleeve and inner sleeve, the inside of inner sleeve is used for device multiple zebra fish embryo / fry, and a magnetic bead is placed in the inner sleeve, the outer sleeve is stored in the centrifuge and carries out centrifugation, the inner sleeve is installed at the top of outer sleeve through the neck pipe, the inner sleeve is equipped with a plurality of through holes from the bottom to the middle extension, the top of outer sleeve is connected with sealing cover, the sealing cover is connected with the neck pipe, so as to seal the neck pipe through the sealing cover, the neck pipe includes short neck pipe and long neck pipe.

[0005] Preferably, the sealing cover is provided with a connecting boss on one side of the outer sleeve.

[0006] Preferably, the short neck pipe and the long neck pipe are arranged from top to bottom in a large-to-small manner, and the top of the short neck pipe and the long neck pipe is connected with the connecting boss.

[0007] Preferably, the top of the neck pipe is located at the top of the outer sleeve and is supported by the top of the outer sleeve, and the top of the inner sleeve is located at the bottom of the neck pipe and is supported by the bottom of the neck pipe.

[0008] Preferably, the sealing cover is provided with an extension block on one side.

[0009] Preferably, when the bottom of the connecting boss extends into the inner part of the short neck pipe or the long neck pipe, it is in contact with the top of the inner sleeve.

[0010] Compared with the prior art, the utility model has the beneficial effects that:

[0011] The inner sleeve, the short neck pipe and the outer sleeve are integrally matched with the centrifuge, the fish culture water / fry culture solution is removed, and the bottom of the inner sleeve is provided with zebra fish embryos / fry without water; then the inner sleeve, the long neck pipe and the outer sleeve are integrally matched with the automatic grinder, the zebra fish embryos / fry in the inner sleeve are inserted into the nucleic acid / protein extraction solution (such as TRIzol lysis solution) and are fully ground and extracted; the inner sleeve, the short neck pipe and the outer sleeve are integrally matched with the centrifuge again, the broken zebra fish embryos / fry tissue in the inner sleeve is collected into the bottom of the outer sleeve, a nucleic acid / protein mixed solution is obtained, and the nucleic acid / protein solution can be obtained by centrifuging and taking the supernatant of the outer sleeve. BRIEF DESCRIPTION OF DRAWINGS

[0012] Fig. 1 is the inner sleeve and the short neck pipe connection structure schematic view of an embodiment of the utility model;

[0013] Fig. 2 is the inner sleeve and the long neck pipe connection structure schematic view of an embodiment of the utility model;

[0014] Fig. 3 is the zebra fish embryo / fry tissue protein / nucleic acid extraction process schematic view of an embodiment of the utility model.

[0015] In the drawing label: 1, outer sleeve; 2, inner sleeve; 3, magnetic bead; 4, through hole; 5, sealing cover; 6, connecting boss; 7, short neck pipe; 8, long neck pipe; 9, extension block. DETAILED DESCRIPTION

[0016] The utility model is further explained in detail in connection with the drawings and embodiments. Same parts are indicated with same drawing labels in the following description. It should be noted that the words "front", "back", "left", "right", "up" and "down" used in the following description refer to the directions in the drawings, and the words "bottom" and "top", "inner" and "outer" respectively refer to the directions towards or away from the geometric center of a specific part.

[0017] Embodiment: please refer to Figs. 1-3The utility model provides a zebra fish embryo, young fish's tissue protein, nucleic acid extraction sleeve, including outer sleeve 1 and inner sleeve 2, the inside of inner sleeve 2 is used for device multiple zebra fish embryo / young fish, and the inner sleeve 2 inside places a magnetic bead 3, and the magnetic bead 3 is used to grind and crush multiple zebra fish embryo / young fish collected, and the inner sleeve 2 is installed at the top of outer sleeve 1 through neck pipe, and the inner sleeve 2 extends from the bottom to the middle and is equipped with multiple through -holes 4, and the outer sleeve 1 is stored in centrifuge and is centrifuged, so that the mixed solution of nucleic acid / protein after crushing flows into the bottom of outer sleeve 1 through through -hole 4, and the top of outer sleeve 1 is connected with sealing cover 5, and sealing cover 5 is connected with neck pipe, so as to seal neck pipe through sealing cover 5, and the neck pipe includes short neck pipe 7 and long neck pipe 8, and the inner sleeve 2 is installed in outer sleeve 1 in short neck pipe 7 and long neck pipe 8 in proper order, and the whole cooperation of inner sleeve 2, short neck pipe 7 and outer sleeve 1 is used first in centrifuge, so that the inner bottom of outer sleeve 1 obtains anhydrous zebra fish embryo / young fish, and the whole cooperation of inner sleeve 2, long neck pipe 8 and outer sleeve 1 is used after, and automatic grinder is used, so that the anhydrous zebra fish embryo / young fish in inner sleeve 2 is inserted into nucleic acid / protein extraction solution and grinds fully, and the whole cooperation of inner sleeve 2, short neck pipe 7 and outer sleeve 1 is used again in centrifuge, so that the mixed solution of nucleic acid / protein extracted is converged into outer sleeve 1, and nucleic acid / protein solution can be obtained by centrifuging and taking supernatant of outer sleeve 1, improve the extraction efficiency of tissue protein / nucleic acid.

[0018] In one or more embodiments of the utility model, the sealing cover 5 is provided with a connecting boss 6 on one side of the outer sleeve 1.

[0019] In one or more embodiments of the utility model, the short neck pipe 7 and the long neck pipe 8 are arranged from top to bottom in a large-to-small manner, and the top of the short neck pipe 7 and the long neck pipe 8 is connected with the connecting boss 6, thereby improving the sealing effect between the sealing cover 5 and the neck pipe.

[0020] In one or more embodiments of the utility model, the top of the neck pipe is located at the top of the outer sleeve 1 and is supported by the top of the outer sleeve 1, and the top of the inner sleeve 2 is located at the bottom of the neck pipe and is supported by the bottom of the neck pipe.

[0021] In one or more embodiments of the utility model, one side of the sealing cover 5 is provided with an extension block 9, which facilitates opening of the sealing cover 5 through the extension block 9.

[0022] In one or more embodiments of the utility model, when the bottom of the connecting boss 6 extends into the inner part of the short neck pipe 7 or the long neck pipe 8, it is in contact with the top of the inner sleeve 2, so that the inner sleeve 2 is fixed in position in the outer sleeve 1 when the connecting boss 6 extends into the inner part of the short neck pipe 7 or the long neck pipe 8, thereby improving the stability of the sleeve during centrifugation and avoiding shaking of the inner sleeve 2 in the outer sleeve 1.

[0023] In use, first, the plurality of zebrafish embryos / fry and the fry culture solution are loaded into the inner sleeve 2, then the bottom end of the inner sleeve 2 is extended from the bottom of the short neck tube 7, and the bottom of the short neck tube 7 supports the top of the inner sleeve 2, then the whole after the short neck tube 7 and the inner sleeve 2 are connected is placed in the outer sleeve 1, and the top of the outer sleeve 1 supports the top of the short neck tube 7, then the whole after the short neck tube 7, the inner sleeve 2 and the outer sleeve 1 are connected is stored in the centrifuge for centrifugation, so that the fry culture solution flows into the bottom of the outer sleeve 1 through the through hole 4, then the short neck tube 7 and the inner sleeve 2 are taken out, the fry culture solution at the bottom of the outer sleeve 1 is poured out, the bottom end of the inner sleeve 2 is extended from the bottom of the long neck tube 8, and the bottom of the long neck tube 8 supports the top of the inner sleeve 2, then the whole after the long neck tube 8 and the inner sleeve 2 are connected is placed in the outer sleeve 1, and the top of the outer sleeve 1 supports the top of the short neck tube 7, so that the water-free zebrafish embryo / fry tissue in the inner sleeve 2 extends into the nucleic acid / protein extraction solution, and the magnetic beads 3 in the automatic grinder are rotated in the inner sleeve 2 to grind the collected plurality of zebrafish embryos / fry, so that the broken nucleic acid / protein mixed solution flows into the bottom of the outer sleeve 1 through the through hole 4, then the long neck tube 8 and the inner sleeve 2 are taken out, the bottom end of the inner sleeve 2 is extended from the bottom of the short neck tube 7, and the bottom of the short neck tube 7 supports the top of the inner sleeve 2, then the whole after the short neck tube 7 and the inner sleeve 2 are connected is placed in the outer sleeve 1, and the top of the outer sleeve 1 supports the top of the short neck tube 7, then the whole after the short neck tube 7, the inner sleeve 2 and the outer sleeve 1 are connected is stored in the centrifuge for secondary centrifugation, so that the broken zebrafish embryo / fry tissue in the inner sleeve 2 flows into the bottom of the outer sleeve 1 through the through hole 4, and the supernatant is taken out by centrifuging the outer sleeve 1 to obtain the nucleic acid / protein solution, thereby improving the extraction efficiency of the tissue protein / nucleic acid.

[0024] It is obvious to those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be regarded as exemplary and non-limiting, and the scope of the present application is defined by the appended claims rather than the above description, and therefore all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present application. Any reference signs in the claims should not be regarded as limiting the claims involved.

Claims

1. A tube for extracting tissue proteins and nucleic acids from zebrafish embryos and juveniles, characterized in that: The device includes an outer tube (1) and an inner tube (2). The inner tube (2) is used to house multiple zebrafish embryos / juveniles, and a magnetic bead (3) is placed inside the inner tube (2). The outer tube (1) is used to store the zebrafish embryos / juveniles in a centrifuge for centrifugation. The inner tube (2) is installed on the top of the outer tube (1) through a neck tube. The inner tube (2) has multiple through holes (4) extending from the bottom to the middle. A sealing cap (5) is connected to the top of the outer tube (1). The sealing cap (5) is connected to the neck tube to seal the neck tube. The neck tube includes a short neck tube (7) and a long neck tube (8).

2. The zebrafish embryo and juvenile fish tissue protein and nucleic acid extraction sleeve according to claim 1, characterized in that: The sealing cap (5) has a connecting boss (6) on one side of the outer sleeve (1).

3. The zebrafish embryo and juvenile fish tissue protein and nucleic acid extraction sleeve according to claim 2, characterized in that: The short neck tube (7) and the long neck tube (8) are arranged from large to small from top to bottom, and the top of the short neck tube (7) and the long neck tube (8) are connected to the connecting boss (6).

4. The zebrafish embryo and juvenile fish tissue protein and nucleic acid extraction sleeve according to claim 2, characterized in that: When the bottom of the connecting boss (6) extends into the short neck tube (7) or the long neck tube (8), it contacts the top of the inner sleeve (2).

5. A tube for extracting tissue proteins and nucleic acids from zebrafish embryos and juveniles according to any one of claims 1-4, characterized in that: The top of the neck tube is located at the top of the outer sleeve (1) and is supported by the top of the outer sleeve (1), while the top of the inner sleeve (2) is located at the bottom of the neck tube and is supported by the bottom of the neck tube.

6. A tube for extracting tissue proteins and nucleic acids from zebrafish embryos and juveniles according to any one of claims 1-4, characterized in that: An extension block (9) is provided on one side of the sealing cover (5).

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