In-situ multi-channel closed water body collection and enrichment fixing device

Abstract

The utility model belongs to the technical field of water body collection and enrichment fixing in marine science and marine engineering, and particularly relates to an in-situ multichannel closed water body collection and enrichment fixing device, which is controlled by a control and power supply system, and is connected with an electric rotating valve, a multichannel valve, a flow meter and a diaphragm pump through a control cable. Communication and switching of multiple channels are achieved, the sampling efficiency and the sampling accuracy are improved, and collection, enrichment and microorganism immobilization of a water body are completed. The diaphragm pump is arranged at the rear, pollution is reduced, single samples are sealed and stored by the multi-channel valve to increase protection of the samples, meanwhile, the functions of enrichment filtering, sample fixing and water body collecting are achieved, and the device can be widely applied to water body samples needing to be strictly sampled. The device meets the requirements of high-flux filtration, fixation and water body direct collection of the ocean water body, and can be used for researching microorganisms in the ocean water body.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the water body collection and enrichment fixing technical field in the ocean science and ocean engineering, concretely is a kind of in-situ multi-channel closed water body collection and enrichment fixing device. BACKGROUND

[0002] Marine microbial community is an important part of the marine biosphere, and plays an important driving role in the marine geochemical cycle. The carbon, nitrogen and sulfur cycles driven by marine microorganisms play a very important role in the global carbon flux changes, ultimately changing the content of carbon dioxide and oxygen in the atmosphere. The study of the metabolic coupling mechanism of marine microorganisms is of great significance to understanding the long-term evolution of the earth's climate. In addition, marine microorganisms have special life processes due to long-term extreme environmental selection; therefore, the study of marine microorganisms is an important foundation for future development of marine biological resources.

[0003] Sampling has become the primary task in marine microbiology research. Currently, there are corresponding products in China for in-situ sampling and enrichment culture of marine microorganisms. Through in-situ sampling and enrichment culture, many unique bacteria from the marine environment have been discovered and isolated. Existing underwater equipment is divided into direct-access water body equipment, in-situ enrichment filtration sampling equipment, and in-situ enrichment fixation equipment, etc., and there are many types, including open-top marine microbial culture chambers, in-situ marine microbial enrichment devices, eight-channel marine in-situ microbial culture devices, grid-type microbial culture devices, large-volume water sample filtration and sampling systems from McLane Corporation of the United States, marine hydrothermal vent microbial filtration and sampling devices, multi-stage membrane filtration pressure-holding samplers, in-situ microbial filtration fixation devices developed by the Institute of Deep-Sea Science and Engineering of the Chinese Academy of Sciences, and in-situ time-segmented sampling and filtration systems for suspended sediment in water (publication number CN113069807A), etc. Among them, in-situ enrichment and fixation equipment mainly performs enrichment of water and fixation of microorganisms. These include: "A Multi-Sequence Deep-Sea Microorganism In-situ Enrichment and Fixation Sampling Device and Method" (published June 21, 2024, publication number CN118222383A); "A Marine Microorganism Multi-Stage In-situ Enrichment Device and Sampling Method" (published March 5, 2024, publication number CN117645914A); and "An Automated In-situ Enrichment and Fixation Device for Microorganisms Applied to Full Ocean Depth" (announced May 18, 2016, authorization announcement number CN205241694U). All of these devices utilize a main control board to control valves and pumps to complete membrane enrichment and fixation of water. In summary, while the currently proposed devices and methods can achieve in-situ enrichment and fixation, they still have shortcomings. Firstly, the pumps are all pre-pumps. In actual operation, microorganisms or other impurities are fed to the pump before the membrane, causing pump blockage and contamination. Over time, after several filtrations, the structure needs cleaning, which is extremely troublesome and affects the sample's effectiveness. Secondly, direct water acquisition is not possible during the enrichment process, leading to data loss. Thirdly, the multi-channel enrichment unit is connected to the external water body; prolonged underwater placement can contaminate the sample. In conclusion, while there are many enrichment filtration systems, very few simultaneously involve deep-water multi-channel closed-loop enrichment filtration, fixation, and water sampling systems. Summary of the Invention

[0004] To address the aforementioned problems with existing water microbial sampling devices, the purpose of this invention is to provide an in-situ multi-channel closed-loop water collection and enrichment fixation device. This device can simultaneously fulfill three functions—water enrichment membrane, sample fixation, and direct water collection—on a single unit, improving sampling efficiency, functionality, and airtightness.

[0005] The objective of this utility model is achieved through the following technical solution:

[0006] This utility model includes a fixed liquid bag, an electric rotary valve, a water tank, a storage bag, a multi-channel valve, a filter, a flow meter, a diaphragm pump, and a control and power system. The electric rotary valve, multi-channel valve, flow meter, and diaphragm pump are respectively connected to the control and power system. The electric rotary valve has an input end with a normally open port connected to the outside and a fixed port connected to the fixed liquid bag. The output end of the electric rotary valve has an output port, which is connected to either the normally open port or the fixed port via the control and power system. The multi-channel valve has a multi-channel valve input port and a multi-channel... The system includes a multi-channel valve output port and multiple sets of ports. The multi-channel valve input port is connected to the output port of an electric rotary valve. The multi-channel valve output port is sequentially connected to a flow meter and a diaphragm pump. After reaching the water outlet, the water flows to the outside. Each set of ports has two multi-channel valve ports, and at least one set of two multi-channel valve ports is connected to the input and output ends of the filter, respectively. The storage bag is placed inside the water tank, and at least one set of two multi-channel valve ports is connected to the inside of the storage bag and the inside of the water tank, respectively. The multi-channel valve is controlled by a control and power system to connect the multi-channel valve input port and the multi-channel valve output port to any two multi-channel valve ports of any set, thereby switching channels.

[0007] The multi-channel valve includes a housing, a motor, and a valve core. The motor is fixed inside the housing and connected to the control and power system. The valve core is provided with a rotating shaft, and the valve core is rotatably installed inside the housing via the rotating shaft. The output end of the motor is connected to the rotating shaft. The valve core and the inside of the housing are provided with an inlet / outlet structure that connects the multi-channel valve inlet and outlet port to any two ports of the multi-channel valve in any group.

[0008] The inlet and outlet structure includes an inlet annular channel, an outlet annular channel, an inlet pipe, and an outlet pipe. The inlet annular channel and the outlet annular channel are respectively provided inside the housing on the upper and lower sides of the valve core. The inlet port of the multi-channel valve is connected to the inlet annular channel, and the outlet port of the multi-channel valve is connected to the outlet annular channel. The valve core is provided with an inlet pipe and an outlet pipe respectively. One end of the inlet pipe is always connected to the inlet annular channel, and one end of the outlet pipe is always connected to the outlet annular channel. The other end of the inlet pipe and the other end of the outlet pipe are connected to any two ports of the multi-channel valve as the valve core rotates driven by the motor.

[0009] The inner and outer rings of the water inlet annular channel and the inner and outer rings of the water outlet annular channel are all provided with annular channel sealing rings, and the outer rings of the valve core located at the other end of the water inlet pipe and the other end of the water outlet pipe are both provided with pipe sealing rings.

[0010] The top of the outer shell of the water tank is provided with a water inlet, and the interior of the water tank is connected to the outside through the water inlet. The bottom of the water tank is provided with a water outlet that is connected to the interior. The storage bag is provided with a storage bag inlet, and at least one set of two multi-channel valve ports are respectively connected to the storage bag inlet and the water tank outlet.

[0011] Once the device reaches underwater, external water enters the water storage tank under pressure through the water inlet. When any two ports of the multi-channel valve are connected to the inside of the water storage tank and the inside of the storage bag respectively, the water entering the water storage tank flows out from the water storage tank outlet through the action of the diaphragm pump. When the external water entering through the water inlet of the water storage tank is insufficient to replenish the water in the water storage tank, the external water enters the storage bag through the multi-channel valve and the inlet of the storage bag for water collection.

[0012] There is a height difference between the normally open port of the electric rotary valve and the connection point with the outside world and the water outlet.

[0013] When the normally open port of the electric rotary valve is connected to the output port of the electric rotary valve, an electromagnetic signal is generated and transmitted to the control and power system to indicate that the external water pumping has reached its position; when the fixed port of the electric rotary valve is connected to the output port of the electric rotary valve, an electromagnetic signal is generated and transmitted to the control and power system to indicate that the fixed liquid has been extracted to its position.

[0014] The advantages and positive effects of this utility model are as follows:

[0015] 1. This utility model controls the entire device through a control and power system, and uses control cables to connect an electric rotary valve, a multi-channel valve, a flow meter, and a diaphragm pump to realize the connection and switching of multiple channels, thereby improving sampling efficiency and accuracy, and completing the collection, enrichment, and microbial fixation of water.

[0016] 2. This utility model places the diaphragm pump at the rear, reducing the problem of sample contamination caused by the accumulation of impurities in the diaphragm pump.

[0017] 3. In this utility model, the input and output channels between any two multi-channel valve ports in any group are closed, thus providing sealed protection for the enrichment membrane and the water body.

[0018] 4. This utility model can simultaneously possess three functions in one device: enrichment filtration, sample fixation, and water collection. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the structure of the water tank and storage bag of this utility model;

[0021] Figure 3 This is a schematic diagram of the external structure of the multi-channel valve of this utility model;

[0022] Figure 4 This is a schematic diagram of the internal structure of the multi-channel valve of this utility model;

[0023] Wherein: 1 is the fixed liquid bag, 2 is the external environment, 3 is the normally open port of the electric rotary valve, 4 is the electric rotary valve, 5 is the output port of the electric rotary valve, 6 is the fixed port of the electric rotary valve, 7 is the inlet of the storage bag, 8 is the water tank, 9 is the storage bag, 10 is the outlet of the water tank, 11 is the water inlet of the water tank, 12 is the multi-channel valve, 13 is the inlet of the multi-channel valve, 14 is the port of the multi-channel valve, 15 is the outlet of the multi-channel valve, 16 is the filter, 17 is the flow meter, 18 is the diaphragm pump, 19 is the water outlet, 20 is the control cable, 21 is the control and power system, 22 is the motor, 23 is the shaft, 24 is the inlet ring channel, 25 is the valve core, 26 is the inlet pipe, 27 is the outlet pipe, 28 is the outlet ring channel, 29 is the sealing ring, 30 is the pipe sealing ring, 31 is the ring channel sealing ring, and 32 is the housing. Detailed Implementation

[0024] The present invention will now be described in further detail with reference to the accompanying drawings.

[0025] like Figure 1As shown, this utility model includes a fixed liquid bag 1, an electric rotary valve 4, a water tank 8, a storage bag 9, a multi-channel valve 12, a filter 16, a flow meter 17, a diaphragm pump 18, and a control and power system 21. The electric rotary valve 4, multi-channel valve 12, flow meter 17, and diaphragm pump 18 are connected to the control and power system 21 via control cables 20. The input end of the electric rotary valve 4 has a normally open port 3 connected to the outside environment 2 and a fixed port 6 connected to the fixed liquid bag 1. The output end of the electric rotary valve 4 has an output port 5, which is connected to either the normally open port 3 or the fixed port 6 via the control of the control and power system 21. The multi-channel valve 12 has a multi-channel valve input port 1. 3. The multi-channel valve output port 15 and multiple sets of ports are connected. The multi-channel valve input port 13 is connected to the electric rotary valve output port 5. The multi-channel valve output port 15 is connected in sequence to the flow meter 17 and the diaphragm pump 18. After reaching the water body outflow end 19, the water flows to the outside 2. Each set of ports has two multi-channel valve ports 14. At least one set of two multi-channel valve ports 14 are connected to the input end and the output end of the filter 16 respectively to complete the enrichment filtration of the water body. The storage bag 9 is placed in the water storage tank 8. At least one set of two multi-channel valve ports 14 are connected to the inside of the storage bag 9 and the inside of the water storage tank 8 respectively. The multi-channel valve 12 is controlled by the control and power system 21 to connect the multi-channel valve input port 13 and the multi-channel valve output port 15 to any one set of two multi-channel valve ports 14 to switch channels.

[0026] The fixative bag 1 in this embodiment contains RNAlater fixative or other RNA preservation solutions. The preservation solution is a water-soluble, non-toxic tissue storage reagent that can rapidly penetrate into the tissue to stabilize and protect the RNA in the cells. Using RNAlater eliminates the need for immediate tissue sample processing and freezing of the sample in liquid nitrogen for subsequent processing. Collecting tissue blocks and immersing them in RNAlater solution preserves the quality and quantity of RNA obtained during subsequent RNA extraction. RNAlater fixative is prior art; it is disclosed in the following reference: NATURE COMMUNICATIONS (2020) 11:3454 | https: / / doi.org / 10.1038 / s41467-020-17284-4 | www.nature.com / naturecommunications, page 9, paragraph 1.

[0027] The electric rotary valve 4 in this embodiment is a commercially available product, specifically a deep-sea two-position three-way valve purchased from Qingdao Lanchi Technology Co., Ltd. Unlike general-purpose electromagnetic two-position three-way valves, the electric rotary valve 4 uses a motor to rotate and complete the channel switching. When the normally open port 3 of the electric rotary valve is connected to the output port 5, a positioning electromagnetic signal is generated and transmitted to the control and power system 21, indicating the completion of external water pumping. When the fixed port 6 of the electric rotary valve is connected to the output port 5, a positioning electromagnetic signal is generated and transmitted to the control and power system 21, indicating the completion of stationary liquid extraction.

[0028] like Figure 1 and Figure 2 As shown, the storage bag 9 in this embodiment is a soft bag, placed inside the water tank 8. The storage bag 9's volume is limited by the size of its own body and the water tank 8, resulting in a limited water collection volume. In this embodiment, the top of the water tank 8's outer shell has a water inlet 11, through which the interior of the water tank 8 communicates with the outside environment 2. The bottom of the water tank 8 has a water outlet 10 communicating with the interior. The storage bag 9 has a storage bag inlet 7, and at least one set of two multi-channel valve ports 14 are connected to the storage bag inlet 7 and the water tank outlet 10, respectively. The water inlet 11 of the water tank is an opening on the top surface of the water tank 8, which connects to the outside world 2. When the device reaches the underwater level, due to the pressure, the water from the outside world 2 will enter the water tank 8 through the water inlet 11. When any two multi-channel valve ports 14 of any group on the multi-channel valve 12 are connected to the inside of the water tank 8 and the inside of the storage bag 9 respectively, the diaphragm pump 18 will run, and the water entering the water tank 8 will flow out from the water tank outlet 10. When the water from the outside world 2 entering through the water inlet 11 is insufficient to replenish the water in the water tank 8, the water from the outside world 2 will enter the storage bag 9 through the storage bag inlet 7 via the multi-channel valve 12 for water collection.

[0029] like Figure 1 , Figure 3 and Figure 4 As shown, the multi-channel valve 12 in this embodiment includes a housing 32, a motor 22, and a valve core 25. The motor 22 is fixed inside the housing 32 and connected to the control and power system 21. The valve core 25 is provided with a rotating shaft 23, and the valve core 25 is rotatably mounted inside the housing 32 via the rotating shaft 23. The output end of the motor 22 is connected to the rotating shaft 23. The valve core 25 and the inside of the housing 32 are provided with an inlet / outlet structure that connects the multi-channel valve inlet 13, the multi-channel valve outlet 15, and any two multi-channel valve ports 14 of a set. In this embodiment, the valve core 25 extends outward from the upper and lower sides at the middle position to form a rotating shaft 23, and a sealing ring 29 is provided on the outside of each rotating shaft 23. The multi-channel valve inlet 13 is the inlet port of water, and the multi-channel valve outlet 15 is the outlet port of water. Any two multi-channel valve ports 14 of a set are opened and closed correspondingly to complete the opening and closing of each channel and complete the sealing and preservation.

[0030] The water inlet and outlet structure of this embodiment includes an inlet annular channel 24, an outlet annular channel 28, an inlet pipe 26, and an outlet pipe 27. The inlet annular channel 24 and the outlet annular channel 28 are respectively provided inside the housing 32 on the upper and lower sides of the valve core 25. The multi-channel valve inlet port 13 is connected to the inlet annular channel 24, and the multi-channel valve outlet port 15 is connected to the outlet annular channel 28. The valve core 25 is provided with an inlet pipe 26 and an outlet pipe 27 respectively. One end of the inlet pipe 26 is always connected to the inlet annular channel 24, and one end of the outlet pipe 27 is always connected to the outlet annular channel 28. The other end of the inlet pipe 26 and the other end of the outlet pipe 27 are connected to any two multi-channel valve ports 14 during the process of the valve core 25 being driven to rotate by the motor 22. The inner and outer rings of the inlet annular channel 24 and the inner and outer rings of the outlet annular channel 28 are all provided with annular channel sealing rings 31, and the outer rings of the valve core 25 located at the other end of the inlet pipe 26 and the other end of the outlet pipe 27 are all provided with pipe sealing rings 30.

[0031] The filter 16 in this embodiment is a prior art, using a general-purpose filter. The pore size can be selected according to actual experimental requirements, and the external dimensions can be 142mm, 90mm, 47mm, etc.

[0032] The flow meter 17 in this embodiment is a commercially available product, specifically an impeller flow meter, model UN-FL-ME-050, purchased from Qingdao Lanchi Technology Co., Ltd. The flow meter 17 transmits the flow signal to the control and power system 21 to complete the calculation of water body sampling and enrichment filtration flux by the filter 16.

[0033] The control and power system 21 is the central control system of the entire device. Using the underwater communication cable (i.e., control cable 20), it can control the electric rotary valve 4, multi-channel valve 12, flow meter 17, and diaphragm pump 18, completing control and coordination tasks and fulfilling the operational process. The control and power system 21 in this embodiment is existing technology and will not be described further here.

[0034] In this embodiment, both the external environment 2 and the water outlet 19 are external water bodies. To prevent mutual interference, they are not placed on the same horizontal plane as much as possible. That is, there is a height difference between the normally open port 3 of the electric rotary valve and the external environment 2 and the water outlet 19.

[0035] This utility model relates to an in-situ multi-channel closed-loop water body collection and enrichment fixation method, comprising the following steps:

[0036] Step A: Clean the pipeline and channel ports of the multi-channel valve 12 with alcohol at the shore-based end, purge the air from the storage bag 9, and then place it in the water tank 8. Put the RNA fixative into the fixative bag 1, and set the control and power system 21 according to the experimental requirements to complete the corresponding process.

[0037] Step B: Use a CTD (Conductivity, Temperature, Depth) or a deep-sea submersible to carry an in-situ multi-channel closed-loop water body collection and enrichment fixation device to the corresponding depth for operation;

[0038] Step C, the control and power system 21 controls the electric rotary valve 4 to connect the normally open port 3 of the electric rotary valve 4 to the output port 5 of the electric rotary valve, and connects any two multi-channel valve ports 14 of any group on the multi-channel valve 12 to the filter 16.

[0039] Step D: The control and power system 21 controls the diaphragm pump 18 as the power source. The water from the outside 2 flows through the normally open port 3 of the electric rotary valve, the output port 5 of the electric rotary valve, the inlet 13 of the multi-channel valve, the filter 16, the output port 15 of the multi-channel valve, the flow meter 17, and the diaphragm pump 18 to the water outlet 19 for water enrichment. The enrichment amount is counted by the flow meter 17, and the process stops after the experimental requirements are met.

[0040] Step E: If filtration fixation is required, the control and power system 21 controls the electric rotary valve 4 to connect the electric rotary valve fixed port 6 and the electric rotary valve output port 5; the control and power system 21 controls the diaphragm pump 18 as a power source, and the fixative in the fixative bag 1 passes through the electric rotary valve fixed port 6, the electric rotary valve output port 5, the multi-channel valve inlet 13, the filter 16, the multi-channel valve output port 15, the flow meter 17, and the diaphragm pump 18 to reach the water outlet 19 for membrane fixation; the membrane fixation volume is counted by the flow meter 17, and stops after the experimental requirements are met;

[0041] Step F: When water collection is required, the control and power system 21 controls the normally open port 3 of the electric rotary valve 4 to connect with the output port 5 of the electric rotary valve, and the two multi-channel valve ports 14 of any group on the multi-channel valve 12 to connect with the inlet 7 of the storage bag and the outlet 10 of the water tank; the control and power system 21 controls the diaphragm pump 18 as a power source, and the water in the water tank 8 reaches the water body through the outlet 10 of the water tank, the multi-channel valve ports 14 and 15, the multi-channel valve outlet 17, and the diaphragm pump 18. Outlet 19 is used for water discharge. During operation, when the water in the storage tank 8 is discharged, a negative pressure is formed. Water from the outside 2 enters the storage bag 9 through the normally open port 3 of the electric rotary valve, the output port 5 of the electric rotary valve, the inlet 13 of the multi-channel valve, the port 14 of the multi-channel valve, and the inlet 7 of the storage bag for storage. The amount of water in the storage bag 9 is counted by the flow meter 17. Usually, a margin of 10% is set (that is, the value counted by the flow meter is 10% more than the amount of water collected in the storage bag). The operation stops after the experimental requirements are met.

[0042] Step G: The control and power system 21 controls the electric rotary valve 4 to complete the switching of each port, and repeats the operation of steps C to F.

[0043] Step H: After completing the work, the vessel will reach the shore base to collect water samples and membranes; then it will be cleaned with alcohol to complete the overall operation.

[0044] This invention features a simple structure and control method, with a post-positioned diaphragm pump reducing contamination and a multi-channel valve for sealing individual samples, enhancing sample protection. It also integrates enrichment filtration, sample fixation, and water collection functions, making it widely applicable to water samples requiring stringent sampling. This invention meets the high-throughput filtration, fixation, and direct water collection needs of marine water bodies and can be used for marine microbiological research.

Claims

1. An in-situ multi-channel closed-loop water collection and enrichment device, characterized in that: The system includes a fixed liquid bag (1), an electric rotary valve (4), a water tank (8), a storage bag (9), a multi-channel valve (12), a filter (16), a flow meter (17), a diaphragm pump (18), and a control and power system (21). The electric rotary valve (4), the multi-channel valve (12), the flow meter (17), and the diaphragm pump (18) are connected to the control and power system (21). The input end of the electric rotary valve (4) has a normally open port (3) connected to the outside (2) and a fixed port (6) connected to the fixed liquid bag (1). The output end of the electric rotary valve (4) has an output port (5). The output port (5) is connected to the normally open port (3) or the fixed port (6) of the electric rotary valve through the control of the control and power system (21). The multi-channel valve (12) has a multi-channel valve input port ( 13) Multi-channel valve output port (15) and multiple sets of ports. The multi-channel valve input port (13) is connected to the electric rotary valve output port (5). The multi-channel valve output port (15) is connected to the flow meter (17) and the diaphragm pump (18) in sequence. After reaching the water body outflow end (19), it flows to the outside (2). Each set of ports has two multi-channel valve ports (14). At least one set of two multi-channel valve ports (14) are connected to the input end and the output end of the filter (16) respectively. The storage bag (9) is placed in the water storage tank (8). At least one set of two multi-channel valve ports (14) are connected to the inside of the storage bag (9) and the inside of the water storage tank (8) respectively. The multi-channel valve (12) is connected to the multi-channel valve input port (13), the multi-channel valve output port (15) and any two multi-channel valve ports (14) of any set through the control and power system (21) to switch channels.

2. The in-situ multi-channel closed-loop water collection and enrichment device according to claim 1, characterized in that: The multi-channel valve (12) includes a housing (32), a motor (22) and a valve core (25). The motor (22) is fixed inside the housing (32) and connected to the control and power system (21). The valve core (25) is provided with a rotating shaft (23). The valve core (25) is rotatably installed inside the housing (32) through the rotating shaft (23). The output end of the motor (22) is connected to the rotating shaft (23). The valve core (25) and the inside of the housing (32) are provided with an inlet and outlet structure that connects the multi-channel valve inlet port (13), the multi-channel valve outlet port (15) to any two multi-channel valve ports (14) of any group.

3. The in-situ multi-channel closed-loop water collection and enrichment device according to claim 2, characterized in that: The water inlet and outlet structure includes an inlet annular channel (24), an outlet annular channel (28), an inlet pipe (26), and an outlet pipe (27). The inlet annular channel (24) and the outlet annular channel (28) are respectively provided inside the housing (32) on the upper and lower sides of the valve core (25). The multi-channel valve inlet port (13) is connected to the inlet annular channel (24), and the multi-channel valve outlet port (15) is connected to the outlet annular channel (28). The valve core (25) is provided with an inlet pipe (26) and an outlet pipe (27). One end of the inlet pipe (26) is always connected to the inlet annular channel (24), and one end of the outlet pipe (27) is always connected to the outlet annular channel (28). The other end of the inlet pipe (26) and the other end of the outlet pipe (27) are connected to any two multi-channel valve ports (14) during the rotation of the valve core (25) driven by the motor (22).

4. The in-situ multi-channel closed-loop water collection and enrichment device according to claim 3, characterized in that: The inner and outer rings of the water inlet annular channel (24) and the inner and outer rings of the water outlet annular channel (28) are all provided with annular channel sealing rings (31), and the outer rings of the valve core (25) located at the other end of the water inlet pipe (26) and the other end of the water outlet pipe (27) are all provided with pipe sealing rings (30).

5. The in-situ multi-channel closed-loop water collection and enrichment device according to claim 1, characterized in that: The top of the outer shell of the water tank (8) is provided with a water tank inlet (11). The interior of the water tank (8) is connected to the outside (2) through the water tank inlet (11). The bottom of the water tank (8) is provided with a water tank outlet (10) connected to the interior. The storage bag (9) is provided with a storage bag inlet (7). At least one set of two multi-channel valve ports (14) are connected to the storage bag inlet (7) and the water tank outlet (10) respectively.

6. The in-situ multi-channel closed-loop water collection and enrichment device according to claim 5, characterized in that: When the device reaches underwater, the water from the outside (2) enters the water storage tank (8) under pressure through the water tank inlet (11). When any two multi-channel valve ports (14) of any group on the multi-channel valve (12) are connected to the inside of the water storage tank (8) and the inside of the storage bag (9) respectively, the water entering the water storage tank (8) flows out from the water storage tank outlet (10) through the action of the diaphragm pump (18). When the water from the outside (2) entering through the water tank inlet (11) is insufficient to replenish the water in the water storage tank (8), the water from the outside (2) enters the storage bag (9) through the multi-channel valve (12) and the storage bag inlet (7) for water collection.

7. The in-situ multi-channel closed-loop water collection and enrichment device according to claim 1, characterized in that: There is a height difference between the normally open port (3) of the electric rotary valve and the connection point between the outside (2) and the water outlet (19).

8. The in-situ multi-channel closed-loop water collection and enrichment device according to claim 1, characterized in that: When the normally open port (3) of the electric rotary valve is connected to the output port (5) of the electric rotary valve, an electromagnetic signal is generated and transmitted to the control and power system (21) to complete the external water pumping in place indication; when the fixed port (6) of the electric rotary valve is connected to the output port (5) of the electric rotary valve, an electromagnetic signal is generated and transmitted to the control and power system (21) to complete the fixed liquid extraction in place indication.

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