Culture device

Abstract

The utility model provides a culture device which comprises a support and a culture bottle, a sliding block is arranged on the support in a sliding mode, the culture bottle is placed on the support, and the sliding block is used for enabling the culture bottle to incline; a plurality of partition plates are sequentially arranged in an inner cavity of the culture bottle in the direction from the bottle bottom to the bottle opening, the height of each partition plate is smaller than that of the inner cavity, and the heights of the partition plates in the direction from the bottle bottom to the bottle opening are sequentially reduced. According to the utility model, cells with various densities can be simultaneously cultured in one container.

Description

TECHNICAL FIELD

[0001] The utility model relates to immune cell culture technical field, especially a culture device. BACKGROUND

[0002] Immune cells refer to cells involved in or related to immune response. They include lymphocytes, NK cells, monocytes / macrophages, granulocytes, mast cells, etc. Immune cells can be divided into many types, and various immune cells play important roles in the human body. Immune cell in vitro expansion culture usually uses cytokines to activate cells, and immune cells grow in clusters. When at rest, the cell clusters will settle under the action of gravity. When culturing immune cell samples in the prior art, the sample needs to be frequently changed in the early stage of activation, and the cell density needs to be strictly controlled during the early stage of sample expansion. The single container and single density require frequent replacement of culture bottles, which increases the risk of contamination.

[0003] The utility model with publication number CN216073856U discloses an immune cell induction special culture bottle, which comprises a bottle body for culturing immune cells. The side wall of the bottle body is sealed. The top of the bottle body is provided with a bottle neck and a gas permeable window. The top bottle opening of the bottle neck is in sealed cooperation with a bottle cap. The bottom of the bottle body is provided with a cell attachment growth body for the growth of immune cells. The surface of the cell attachment growth body is provided with an undulating structure. A sterile filter membrane is arranged in the gas permeable window. However, the above-mentioned culture bottle can only culture cells with a single density in one container. UTILITY MODEL CONTENT

[0004] The utility model aims to solve the defects in the prior art and provides a culture device, which can culture cells with multiple densities in one container.

[0005] To achieve the above-mentioned purpose, the utility model provides a culture device, which comprises a support and a culture bottle.

[0006] A sliding block is slidably arranged on the support. The culture bottle is placed on the support. The sliding block is used to incline the culture bottle.

[0007] A plurality of partitions are arranged in the culture bottle in sequence from the bottom to the top. The height of each partition is less than the height of the inner cavity. The height of the partitions from the bottom to the top decreases in sequence.

[0008] The area between the bottom of the culture bottle and its adjacent partition is the initial inoculation area, which is used for initial factor spreading and inoculation culture. Cell samples are injected into the initial inoculation area of the culture bottle. The initial inoculation area is used for small-volume culture. During the culture process, the culture bottle is placed vertically and gradually injected with culture medium into the initial inoculation area. After the liquid volume of the culture medium can completely cover the initial inoculation area, one end of the culture bottle is placed on the support, and the other end is placed on the sliding block. The culture bottle is inclined until the highest points of the partitions are on a horizontal line.

[0009] The cell sample is left for a period of time, and the cell mass is settled at the bottom of the culture bottle. By slowly changing the position of the sliding block, the angle of the culture bottle is slowly reduced. The upper liquid in the initial inoculation area gradually and slowly overflows the partition adjacent to the bottom of the culture bottle and gradually overflows to other areas except the initial inoculation area, so that the cell sample is cultured in different areas. The number of cell masses in the area from the bottle bottom to the bottle opening is reduced, so that a container can culture cells of multiple densities at the same time, the culture bottle does not need to be replaced for the activation of cells in the early stage, and multiple densities can be cultured and activated at the same time.

[0010] When the fresh culture medium is supplemented, the culture medium can be placed in other areas except the initial inoculation area. The culture medium does not need to be preheated, and is placed in different areas. After the temperature in the incubator is uniform, the cell sample in the initial inoculation area is mixed uniformly, so that the preheating time of the culture medium can be shortened.

[0011] Optionally, the support is provided with an elastic member, one end of the elastic member is connected with the support, and the other end is connected with the sliding block.

[0012] The elastic member is used to provide a pulling force to pull the sliding block, so that the sliding block moves slowly to slowly change the angle of the culture bottle, and the rapid movement of the sliding block is avoided to prevent the cells in the initial inoculation area from being damaged.

[0013] Optionally, the culture bottle comprises a bottle body and a gas-permeable and bacteria-isolating bottle cap, and the bottle opening of the bottle body is matched with the gas-permeable and bacteria-isolating bottle cap.

[0014] The cell sample can be injected into the inner cavity of the bottle body through the bottle opening of the bottle body. The cell sample in the culture bottle exchanges gas through the gas-permeable and bacteria-isolating bottle cap, and the bacteria-isolating bottle cap can prevent bacteria in the air from entering the culture bottle.

[0015] Optionally, the neck of the bottle body is conical, and the end of the neck away from the bottle bottom extends upward.

[0016] When the culture bottle is placed horizontally, the height of the neck and the bottle opening end of the culture bottle is higher than the height of the bottle body, so that the liquid level in the bottle body is lower than the height of the bottle opening. When the culture bottle is placed horizontally, the cell sample can be prevented from leaking out of the bottle opening.

[0017] Optionally, one end of the support is provided with a baffle for blocking the bottom end of the culture bottle to limit the culture bottle.

[0018] Optionally, the sliding block is a triangular sliding block, and the culture bottle is placed on the inclined surface of the sliding block.

[0019] When the highest points of the plurality of partitions are on a horizontal line, the inclined angle of the triangular sliding block is consistent with the inclination degree of the culture bottle. The triangular sliding block is used to maintain the liquid level to be horizontal, and the inclination angle of the culture bottle is slowly reduced after sliding.

[0020] Optionally, several said partitions are equidistantly arranged in the culture bottle.

[0021] Advantages:

[0022] 1. The utility model discloses a support and culture bottle, the area between the bottle bottom of culture bottle and its adjacent partition is initial inoculation area, is used for initial laying factor and inoculation culture. Inject cell sample into the initial inoculation area of culture bottle, and small -size culture uses initial inoculation area, during the culture, culture bottle is placed and gradually injects culture medium to initial inoculation area, when the liquid volume of culture medium can completely cover initial inoculation area, one end of culture bottle is placed on the support, and the other end is placed on the sliding block, and culture bottle is inclined to the highest point of several partitions on a horizontal line.

[0023] The cell sample is static for a period of time, and the cell mass is settled at the bottom of the culture bottle. By slowly changing the position of the sliding block, the inclination angle of the culture bottle is slowly reduced. The upper liquid in the initial inoculation area gradually and slowly overflows the partition adjacent to the bottle bottom of the culture bottle and gradually overflows to the other areas except the initial inoculation area, realizing the partition culture of the cell sample. The number of cell masses in the area from the bottle bottom to the bottle opening is reduced, a container can simultaneously culture cells of multiple densities, the culture bottle does not need to be replaced for activating the cells in the early stage, and multiple densities can be simultaneously cultured and activated and expanded.

[0024] 2. When the fresh culture medium is supplemented, the culture medium can be placed in the other areas except the initial inoculation area, the culture medium does not need to be preheated, is placed in the partition, is mixed with the cell sample in the initial inoculation area after the temperature in the incubator is uniform, and the culture medium preheating time can be shortened. BRIEF DESCRIPTION OF DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained according to the structures shown in the drawings without creative labor for those skilled in the art.

[0026] Figure 1 The structure diagram of the culture device disclosed by the utility model is shown in the figure.

[0027] Figure 2 The top view of the culture bottle in the culture device disclosed by the utility model is shown in the figure.

[0028] The figure shows the following: 1 support; 11 elastic member; 12 baffle; 2 sliding block; 3 culture bottle; 31 partition; 311 first partition; 312 second partition; 313 third partition; 32 bottle body; 33 bottle neck; 34 air-permeable and bacteria-proof bottle cap.

[0029] The realization of the purpose, functional features and advantages of this utility model will be further explained in conjunction with the implementation methods and with reference to the accompanying drawings. Detailed Implementation

[0030] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0031] In the description of this utility model, unless otherwise explicitly defined, terms such as "setting," "installation," and "connection" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this utility model in conjunction with the specific content of the technical solution.

[0032] In the description of this utility model, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this utility model. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0033] See Figure 1 and 2 A culture device according to an embodiment of the present invention includes a support 1 and a culture flask 3:

[0034] The support 1 is slidably provided with a slider 2, the culture bottle 3 is placed on the support 1, and the slider 2 is used to tilt the culture bottle 3.

[0035] The inner cavity of the culture flask 3 is provided with a plurality of partitions 31 in sequence from the bottom to the mouth of the flask. The height of each partition 31 is less than the height of the inner cavity, and the height of the partitions 31 decreases sequentially from the bottom to the mouth of the flask.

[0036] Specifically, the height of each partition 31 is less than the height of the inner cavity. The partitions 31 arranged from the bottom to the mouth of the culture flask 3 are, in sequence, the first partition 311, the second partition 312, and the third partition 313. The area between the bottom of the culture flask 3 and the first partition 311 is the initial inoculation area, which is used for initial seeding and inoculation culture. The heights of the first partition 311, the second partition 312, and the third partition 313 decrease sequentially.

[0037] First, inject the cell sample into the initial inoculation area of the culture bottle 3, and use the bottom area for small-volume culture; during the culture process, the culture bottle 3 is placed vertically and the culture medium is gradually injected into the initial inoculation area, and after the liquid volume of the culture medium can completely cover the initial inoculation area, the culture bottle 3 is placed on the support 1 at one end and on the sliding block 2 at the other end, and the culture bottle 3 is inclined until the highest point of the several partitions 31 is on a horizontal line;

[0038] The cell sample is allowed to stand for a period of time, and after the cell clumps are settled at the bottom of the culture bottle 3, the inclination angle of the culture bottle 3 is slowly reduced by slowly changing the position of the sliding block 2, and the upper liquid in the initial inoculation area gradually and slowly overflows the first partition 311 and the second partition 312, and flows to the area between the first partition 311 and the second partition 312 on the right, and the area between the second partition 312 and the third partition 313, thereby realizing partition culture of the cell sample, and the number of cell clumps in the areas from left to right decreases, so that a container can be used to culture cells of multiple densities at the same time, the culture bottle does not need to be replaced for early activation of the cells, and multiple densities can be cultured at the same time.

[0039] When the fresh culture medium is supplemented, the culture medium can be placed in the area between the first partition 311 and the second partition 312, or the area between the second partition 312 and the third partition 313, and the culture medium does not need to be preheated, is placed in partitions, and is mixed with the cell sample in the initial inoculation area after the temperature in the incubator is uniform, so that the preheating time of the culture medium can be shortened.

[0040] Referring to Figure 1 In some embodiments of the present application, the support 1 is provided with an elastic member 11, one end of the elastic member 11 is connected with the support 1, and the other end is connected with the sliding block 2.

[0041] The elastic member 11 is used to provide a pulling force to pull the sliding block 2, so as to ensure that the sliding block 2 moves slowly and the inclination angle of the culture bottle 3 changes slowly, thereby avoiding the rapid movement of the sliding block 2 to cause damage to the cells in the initial inoculation area.

[0042] Referring to Figure 1 and 2 In some embodiments of the present application, the culture bottle 3 comprises a bottle body 32 and a gas-permeable bacteria isolation bottle cap 34, and the bottle opening of the bottle body 32 is matched with the gas-permeable bacteria isolation bottle cap 34.

[0043] The cell sample can be injected into the inner cavity of the bottle body 32 through the bottle opening of the bottle body 32, the cell sample in the culture bottle 3 exchanges gas through the gas-permeable bacteria isolation bottle cap 34, and the bacteria and the like in the air can be prevented from entering the culture bottle 3 by the bacteria isolation bottle cap 34.

[0044] Referring to Figure 1 In some embodiments of the present application, the bottle neck 33 of the bottle body 32 is conical, and the end of the bottle neck 33 away from the bottle bottom extends upward.

[0045] When the culture bottle 3 is placed horizontally, the height of the bottle neck 33 and the bottle mouth end of the culture bottle 3 is higher than the height of the bottle body 32, so that the liquid level in the bottle body 32 is lower than the height of the bottle mouth, thereby preventing the cell sample from leaking out of the bottle mouth.

[0046] Referring to Figure 1 In some embodiments of the present application, the support 1 is provided with a baffle 12 at one end, which is used to block the bottle bottom end of the culture bottle 3, so as to limit the culture bottle 3.

[0047] Referring to Figure 1 In some embodiments of the present application, the sliding block 2 is a triangular sliding block, and the culture bottle 3 is placed on the inclined surface of the sliding block 2.

[0048] When the highest points of the plurality of partitions 31 are on a horizontal line, the inclined angle of the triangular sliding block is consistent with the inclination degree of the culture bottle 3, the triangular sliding block is used to maintain the liquid level horizontal, and the inclination angle of the culture bottle 3 is slowly reduced after sliding.

[0049] Referring to Figure 1 and 2 In some embodiments of the present application, the plurality of partitions 31 are equidistantly arranged in the culture bottle 3.

[0050] The above is only the preferred embodiments of the present application, and does not limit the patent scope of the present application, and any equivalent structural transformation, direct / indirect application in other related technical fields under the utility model concept of the present application, and the contents of the present application specification and drawings are included in the patent protection scope of the present application.

Claims

1. A culture device, characterized by, The culture bottle (3) is placed on the support (1), and the sliding block (2) is used to incline the culture bottle (3). A plurality of baffle plates (31) are arranged in the inner cavity of the culture bottle (3) from the bottom to the mouth of the bottle. The support (1) is provided with an elastic element (11), one end of the elastic element (11) is connected with the support (1), and the other end is connected with the sliding block (2).

2. The culture device of claim 1, wherein The culture bottle (3) comprises a bottle body (32) and a breathable and bacteria-proof bottle cap (34), and the bottle mouth of the bottle body (32) is matched with the breathable and bacteria-proof bottle cap (34).

3. The culture device of claim 1, wherein The neck (33) of the bottle body (32) is conical, and the end of the neck (33) away from the bottom of the bottle extends upward.

4. The culture device of claim 3, wherein The sliding block (2) is a triangular sliding block, and the culture bottle (3) is placed on the inclined surface of the sliding block (2).

5. The culture device of claim 1, wherein One end of the support (1) is provided with a baffle (12).

6. The culture device according to any one of claims 1 to 5, wherein A plurality of baffle plates (31) are arranged in the culture bottle (3) at equal intervals.

7. The culture device according to any one of claims 1 to 5, wherein ​

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