Single-hole multi-channel fluorescence detection system based on no optical fiber

Abstract

The utility model discloses a single-hole multi-channel fluorescence detection system based on no optical fiber, which belongs to the field of medical examination and inspection instruments and molecular diagnosis and detection instruments, and has the technical key points that an exciting light assembly and a receiving light assembly of an optical channel module are respectively placed in an exciting light mounting channel and a receiving light mounting channel of a base body; 2n light through holes are uniformly distributed in the circumferential direction of the reaction tank tube seat; the 2n light through holes correspond to the n excitation light assemblies and the n receiving light assemblies in direction. The utility model aims to provide a single-hole multi-channel fluorescence detection system based on no optical fiber, which does not need an optical fiber to transmit an optical signal, so that the miniaturization of an instrument is realized.

Description

TECHNICAL FIELD

[0001] The utility model relates to a kind of medical examination and testing instrument, molecular diagnostic detection instrument field this technical field, specifically related to a kind of single-hole multi-channel fluorescence detection system based on no optical fiber. BACKGROUND

[0002] Fluorescence PCR technology is a nucleic acid detection technology, which detects PCR amplification products through fluorescently labeled probes or dyes. In recent years, fluorescence PCR technology has made significant progress. The design of this system for no optical fiber detection simplifies the instrument structure, reduces the cost, and improves the stability and maintenance convenience of the system. Miniaturization and cost reduction of PCR instrument have become an important development direction.

[0003] The research and development team searched for no optical fiber detection, and the existing technology is summarized as follows:

[0004] Document 1: CN114460056A (Suzhou Yare Biological Technology Co., Ltd.) proposes a linear scanning type fluorescence detection system based on no optical fiber, PCR instrument.

[0005] Document 2: CN211263187U (Suzhou Danbao Medical Technology Co., Ltd.) proposes a no optical fiber type multi-channel CCD fluorescence detection system,

[0006] The above two devices are mainly used for batch detection, so they are relatively large in size and relatively high in cost. In order to meet market demand, a new fluorescence detection system with smaller size and lower cost needs to be developed. UTILITY MODEL CONTENT

[0007] The utility model aims at the deficiency of the prior art, and provides a single-hole multi-channel fluorescence detection system based on no optical fiber.

[0008] The technical solution of the utility model is as follows:

[0009] A single-hole multi-channel fluorescence detection system based on no optical fiber, comprising: a base body, n optical channel modules, and a reaction pool tube seat; n represents a natural number greater than or equal to 2;

[0010] The base body includes a reaction pool mounting hole, n excitation light mounting channels, and n receiving light mounting channels; the n excitation light mounting channels and the n receiving light mounting channels are distributed in a ring around the reaction pool mounting hole.

[0011] The optical channel module comprises: an excitation light assembly, a receiving light assembly, and a light source heat sink; the excitation light assembly comprises, in sequence according to the direction of light advancement, a light source, a first collimating lens, a first filter, and a second collimating lens; the receiving light assembly comprises, in sequence according to the direction of light advancement, a focusing lens, a receiving filter, and a photoelectric sensor.

[0012] The excitation light assembly and the receiving light assembly of the optical channel module are respectively placed in the excitation light mounting channel and the receiving light mounting channel of the base body.

[0013] The upper part of the reaction cell tube seat is open; the circumferential direction of the reaction cell tube seat is uniformly distributed with 2n light through holes; the 2n light through holes correspond to the directions of the n excitation light assemblies and the n receiving light assemblies respectively.

[0014] Further, the reaction cell mounting hole is arranged at the center of the base body, and the n excitation light mounting channels and the n receiving light mounting channels are distributed in a circular array with the reaction cell mounting hole as the center.

[0015] Further, n is 4.

[0016] Further, the directions of the excitation light assembly and the receiving light assembly of each optical channel module form an included angle of 90°.

[0017] Further, the optical channel module further comprises: a light source heat sink; the light source heat sink is arranged outside the light source.

[0018] Further, the light source is an LED lamp.

[0019] Further, an optical top cover is further arranged at the top of the base body; the optical top cover is arranged at the upper part of the base body and is adapted thereto; the middle part of the optical top cover is provided with an opening, and the upper part of the reaction cell tube seat can pass through the opening.

[0020] The application has the following advantages:

[0021] Firstly, the application can realize the following two advantages: (1) miniaturization of the instrument; (2) cost reduction, promoting the development of molecular diagnostic technology.

[0022] Second, the core design of the application is that: the excitation light assembly and the receiving light assembly of the optical channel module are respectively placed in the excitation light mounting channel and the receiving light mounting channel of the base body; the circumferential direction of the reaction pool tube seat is uniformly distributed with 2n light transmission holes; the 2n light transmission holes correspond to the directions of the n excitation light assemblies and the n receiving light assemblies respectively. The reaction tube is placed in the reaction pool tube seat, and the optical channel module works one by one to realize multi-channel detection. Through the foregoing design, the fluorescence detection system of the application no longer needs an optical fiber, thereby realizing the miniaturization of the instrument. BRIEF DESCRIPTION OF DRAWINGS

[0023] The utility model will be further explained in detail in combination with the embodiments in the drawings, but does not constitute any limitation to the utility model.

[0024] Figure 1 It is the elevation view of the single-hole multi-channel fluorescence detection system based on no optical fiber of example one.

[0025] Figure 2 It is the optical structure design drawing of the base body of example one.

[0026] Figure 3 It is the three-dimensional structure design drawing of the optical channel module of example one.

[0027] Figure 4 It is the top view of the optical channel module of example one.

[0028] Figure 5 It is the optical signal schematic diagram of the first optical channel module of example one.

[0029] Figure 6 It is the optical signal schematic diagram of the second optical channel module of example one.

[0030] Figure 7 It is the optical signal schematic diagram of the third optical channel module of example one.

[0031] Figure 8 It is the optical signal schematic diagram of the fourth optical channel module of example one.

[0032] Figure 9 It is the three-dimensional structure design drawing of the reaction pool tube seat of example one.

[0033] Figure 10 It is the three-dimensional structure design drawing of the optical top cover of example one.

[0034] Figures 1-10 The reference signs in the drawings are explained as follows:

[0035] The single-hole multi-channel fluorescence detection system 1000;

[0036] Optical top cover 1100, base body 1200, 4 optical channel modules 1300, reaction pool pipe seat 1400;

[0037] Excitation light installation channel 1201, receiving light installation channel 1202, reaction pool installation hole 1203;

[0038] Excitation light assembly 1310, light source 1311, first collimating lens 1312, first filter 1313, second collimating lens 1314;

[0039] Receiving light assembly 1320, focusing lens 1321, receiving filter 1322, photoelectric sensor 1323;

[0040] Light source heat sink 1330;

[0041] Light ray through hole 1401;

[0042] Open hole 1101. DETAILED DESCRIPTION

[0043] In order to explain the technical content, structural features, purposes and effects of the utility model in detail, the following will be described in detail in combination with the embodiments and the accompanying drawings.

[0044] [Example One] A single-hole multi-channel fluorescence detection system based on no optical fiber

[0045] Figure 1 A three-dimensional structure diagram of a single-hole multi-channel fluorescence detection system 1000 based on no optical fiber is shown. The single-hole multi-channel fluorescence detection system 1000 based on no optical fiber comprises: an optical top cover 1100, a base body 1200, 4 optical channel modules 1300, and a reaction pool pipe seat 1400.

[0046] Figure 2 An optical structure design diagram of the base body is shown. The base body 1200 comprises: a reaction pool installation hole 1203, 4 excitation light installation channels 1201, and 4 receiving light installation channels 1202. The reaction pool installation hole 1203 is arranged at the center of the base body 1200, and the 4 excitation light installation channels 1201 and the 4 receiving light installation channels 1202 are distributed in a circular array with the reaction pool installation hole 1203 as the center.

[0047] Figure 3 、 Figure 4The structural design of the optical channel module 1300 is shown. The optical channel module 1300 includes: excitation light assembly 1310, receiving light assembly 1320, light source heat sink 1330. The excitation light assembly 1310 includes, in order of light propagation direction: light source (LED lamp) 1311, first collimating lens 1312, first filter 1313, second collimating lens 1314. The receiving light assembly 1320 includes, in order of light propagation direction: focusing lens 1321, receiving filter 1322, photoelectric sensor 1323. The light source heat sink 1330 is arranged outside the light source 1311.

[0048] 4 optical channel modules 1300 are respectively called: first optical channel module, second optical channel module, third optical channel module, fourth optical channel module. Figure 5 、 Figure 6 、 Figure 7 、 Figure 8 The optical signal diagrams of the first to fourth optical channel modules 1300 are shown respectively. The excitation light assembly 1310 and the receiving light assembly 1320 of the optical channel module 1300 are placed in the excitation light mounting channel 1201 and the receiving light mounting channel 1202 of the base body 1200 respectively. In particular, the direction angle between the excitation light assembly 1310 and the receiving light assembly 1320 of the same optical channel module 1300 is 90°.

[0049] Figure 9 The three-dimensional structural design of the reaction cell tube seat 1400 is shown. The upper part of the reaction cell tube seat 1400 is open, used for placing the reaction cell. The circumferential direction of the reaction cell tube seat 1400 is uniformly distributed with 8 light through holes 1401, and the 8 light through holes 1401 correspond to the directions of the 4 excitation light assemblies 1310 and the 4 receiving light assemblies 1320.

[0050] Figure 10 The three-dimensional structural design of the optical top cover 1100 is shown. The middle part of the optical top cover 1100 is provided with an opening 1101, and the upper part of the reaction cell tube seat 1400 extends through the opening 1101 to the upper part of the optical top cover 1100. The above design can facilitate the placement of the reaction tube.

[0051] The working process of the single-hole multi-channel fluorescence detection system 1000 based on no optical fiber is as follows:

[0052] S1, the reaction tube is placed in the reaction cell tube seat 1400;

[0053] S2, the first optical channel module is opened and works, and the other three optical channel modules do not work; the light source of the first optical channel module emits excitation light, the excitation light passes through the first collimating lens 1312, the first filter 1313, the second collimating lens 1314, passes through the corresponding opening 1401 of the reaction cell tube seat 1400, and then enters the reaction tube; the receiving light generated by the reaction tube is directed to the photoelectric sensor of the first optical channel module. (In fact: the receiving light generated by the reaction tube is diffused in all directions and has no directivity, that is, the receiving light of the reaction tube will also hit the light source and the photoelectric sensor of the first optical channel module, the second optical channel module, the third optical channel module and the fourth optical channel module; at this time, only the first optical channel module works, that is, only the photoelectric sensor of the first optical channel module can analyze the result).

[0054] S3, similar to S2, the second optical channel module is opened and works, and the other three optical channel modules do not work;

[0055] S4, similar to S2, the third optical channel module is opened and works, and the other three optical channel modules do not work;

[0056] S5, similar to S2, the fourth optical channel module is opened and works, and the other three optical channel modules do not work; at this time, the detection of the reaction tube is completed.

[0057] The above-mentioned embodiments are preferred embodiments of the present application, which are only used to facilitate the description of the present application and do not limit the present application in any form. Any person skilled in the art can make partial changes or modifications to the equivalent embodiments within the scope of the technical features disclosed by the present application without departing from the technical features of the present application, and the changes or modifications still belong to the scope of the technical features of the present application.

Claims

1. A single-hole multi-channel fiberless fluorescence detection system, comprising: The application relates to a reaction pool device, which comprises a base body, n optical channel modules and a reaction pool tube seat. n represents a natural number greater than or equal to 2; The base body comprises a reaction pool mounting hole, n excitation light mounting channels and n receiving light mounting channels; the n excitation light mounting channels and the n receiving light mounting channels are annularly distributed around the reaction pool mounting hole. The optical channel module comprises an excitation light assembly, a receiving light assembly and a light source heat sink; the excitation light assembly comprises a light source, a first collimating lens, a first filter and a second collimating lens in sequence according to the direction of light advancement; the receiving light assembly comprises a focusing lens, a receiving filter and a photoelectric sensor in sequence according to the direction of light advancement. The excitation light assembly and the receiving light assembly of the optical channel module are respectively placed into the excitation light mounting channel and the receiving light mounting channel of the base body. The upper part of the reaction pool tube seat is open; 2n light holes are uniformly distributed in the circumferential direction of the reaction pool tube seat; the 2n light holes correspond to the directions of the n excitation light assemblies and the n receiving light assemblies respectively. The reaction pool mounting hole is arranged at the center of the base body, and the n excitation light mounting channels and the n receiving light mounting channels are circularly arrayed with the reaction pool mounting hole as the center.

2. The single-hole multi-channel fiberless fluorescence detection system according to claim 1, wherein, n is 4.

3. The single-hole multi-channel fiberless fluorescence detection system according to claim 2, wherein, The directions of the excitation light assembly and the receiving light assembly of each optical channel module form an included angle of 90 degrees.

4. The single-hole multi-channel fiberless fluorescence detection system according to claim 3, wherein, The optical channel module further comprises a light source heat sink; the light source heat sink is arranged outside the light source.

5. The single-hole multi-channel fiber-optic-free fluorescence detection system according to any one of claims 1 to 4, wherein, The light source is an LED lamp.

6. The single-hole multi-channel fiber-optic-free fluorescence detection system according to any one of claims 1 to 4, wherein, An optical top cover is further arranged on the top of the base body; the optical top cover is arranged on the upper part of the base body and is adapted to the base body; the middle part of the optical top cover is provided with an opening, and the upper part of the reaction pool tube seat can pass through the opening.

7. The single-hole multi-channel fiber-optic free fluorescence detection system according to any one of claims 1 to 4, wherein, ​

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