Deoxidizing device of anaerobic microorganism reactor
By combining argon cylinders and vacuum pump components with Henry's Law, the problem of dissolved oxygen removal in anaerobic microbial reactors is solved by alternately pumping in and extracting gases, achieving non-destructive deoxygenation and ensuring the activity of microorganisms and the stability of the reaction solution.
Patent Information
- Application Number
- CN202520088586.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-15
- Publication Date
- 2026-02-10
- Estimated Expiration
- 2035-01-15
AI Technical Summary
Existing technologies struggle to effectively remove dissolved oxygen from anaerobic microbial reactors without affecting microbial activity, and commonly used methods may damage the reaction solution.
Using components such as argon cylinders, vacuum pumps, and three-way gas exchange valves, oxygen in the reactor can be removed without heating or chemical reagents by alternating argon injection and extraction, combined with Henry's Law. The potential is measured by forming an electrochemical circuit using a reference electrode and a working electrode.
Without affecting microbial activity and reaction solution composition, dissolved oxygen is completely removed to provide an anaerobic environment, avoiding heating and the use of chemical reagents, thus minimizing the impact on the reaction solution.
Smart Images
Figure CN223892540U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of anaerobic microbial reactor, in particular to an anaerobic microbial reactor oxygen removal device. BACKGROUND
[0002] Anaerobic microbial reactor needs to be allowed in anaerobic or oxygen-free conditions, so after adding the reaction liquid to the reactor, it needs to be deoxygenated. The oxygen in the pipeline and above the reaction liquid can be simply and quickly removed by continuously introducing inert gas into the system, but the oxygen dissolved in the reaction liquid is usually difficult to remove. The commonly used deoxygenation methods in the laboratory include thermal deoxygenation, chemical deoxygenation, vacuum deoxygenation, etc. Thermal deoxygenation is to heat the water to the boiling point, generally 104~105℃. According to Henry's law, at this time, the partial pressure of water vapor on the water surface is almost equal to the total pressure on the water surface, that is, close to atmospheric pressure, so the partial pressure of other gases such as oxygen will tend to zero. This method is very simple and convenient, but at this temperature, most microorganisms cannot survive, so this method cannot be applied to anaerobic microbial reactors. Chemical deoxygenation uses sodium sulfite or disodium sulfite and other chemical reagents to react with oxygen in water to remove oxygen, the disadvantage is that the introduction of other chemical reagents may damage the structure of the microbial nutrient solution, thereby affecting its normal growth. The main principle of vacuum deoxygenation is: according to Henry's law, the pressure above the solution is reduced, and the proportion of gas dissolved in the liquid will also be reduced. Vacuum deoxygenation has little effect on the composition of the reaction liquid, and can also reduce the temperature requirement, but to achieve a more vacuum environment, the water also needs to be preheated, and the preheating temperature needs to reach about 30~50℃. This temperature range is still not friendly to most microorganisms. SUMMARY
[0003] The purpose of the present application is to provide an anaerobic microbial reactor oxygen removal device, which does not need to preheat the reaction liquid in the reactor, and can deoxygenate without affecting the activity of microorganisms, so as to ensure that the anaerobic microbial reactor can normally react.
[0004] The technical scheme adopted by the present application is: an anaerobic microbial reactor oxygen removal device, comprising an argon cylinder, a reactor, a test tube, a vacuum pump, a pressure gauge, a first glass tube, a second glass tube and a three-way gas exchange valve; one end of the first glass tube is connected with the argon cylinder, the other end is connected with the test tube, and a first opening is arranged in the middle of the first glass tube; the first opening is connected with the three-way gas exchange valve; one end of the second glass tube is connected with the three-way gas exchange valve, and the other end is connected with the vacuum pump; one end of the second glass tube connected with the vacuum pump is provided with a second opening, and the second opening is connected with the pressure gauge; and the three-way gas exchange valve is further connected with the reactor.
[0005] Further, the reactor is further provided with a hose valve, and the reactor is connected with the three-way air exchange valve through the hose valve.
[0006] Further, the reactor is further provided with a reference electrode, a working electrode and a counter electrode, and the reference electrode, the working electrode and the counter electrode are all in contact with the microbial reaction solution in the reactor.
[0007] Further, the utility model further includes a gas collecting bottle, and the gas collecting bottle is provided with a valve, and the gas collecting bottle is connected with the vacuum pump through the valve.
[0008] The utility model discloses a reactor, a three-way air exchange valve, a vacuum pump, a pressure gauge, a first glass tube, a second glass tube, a hose valve, a reference electrode, a working electrode and a counter electrode. BRIEF DESCRIPTION OF DRAWINGS
[0009] In order to more clearly illustrate the technical scheme in the embodiment of the utility model, the drawings needed in the embodiment will be briefly introduced as follows, and obviously, the drawings in the following description are only some embodiments of the application, and for those skilled in the art, other drawings can be obtained according to these drawings without creative labor.
[0010] Figure 1 It is the structural schematic diagram of the utility model embodiment.
[0011] Mark explanation: 1 - argon bottle, 2 - reactor, 3 - test tube, 4 - vacuum pump, 5 - pressure gauge, 6 - first glass tube, 7 - second glass tube, 8 - three-way air exchange valve, 9 - first opening, 10 - second opening, 11 - hose valve, 12 - reference electrode, 13 - working electrode, 14 - counter electrode, 15 - gas collecting bottle. DETAILED DESCRIPTION
[0012] In order to more clearly understand the above objectives, features and advantages of the present application, the present application will be further described in detail below with reference to the drawings and specific embodiments. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. However, the present application can be practiced in other ways different from those described herein, and therefore the present application is not limited to the specific embodiments disclosed below.
[0013] Unless otherwise defined, technical terms or scientific terms used herein shall have the ordinary meanings as understood by one of ordinary skill in the art to which this application pertains. The terms "first", "second", and similar terms as used in the description and the claims of the present application do not denote any order, quantity, or importance, but are used to distinguish different components. Similarly, the terms "one" or "a" or "an" do not denote a quantity of one, but rather denote the presence of at least one. The terms "connected" or "coupled" or similar terms as used in the description and the claims of the present application do not denote a direct or mechanical connection, but can include an electrical connection, whether direct or indirect. The terms "upper", "lower", "left", "right", and the like are used only to indicate relative positions, and when the absolute positions of the described objects are changed, the relative positions are also changed accordingly.
[0014] As shown in Figure 1 An anaerobic microorganism reactor deoxidizing device, comprising an argon cylinder, a reactor, a test tube, a vacuum pump, a pressure gauge, a first glass tube, a second glass tube and a three-way air valve. One end of the first glass tube is connected with the argon cylinder, the other end is connected with the test tube, and a first opening is arranged in the middle of the first glass tube. The first opening is connected with the three-way air valve. When the three-way air valve is connected with the argon cylinder and the reactor, argon can be introduced into the reactor to discharge oxygen in the device. One end of the second glass tube is connected with the three-way air valve, the other end is connected with the vacuum pump, and a second opening is arranged in the end of the second glass tube connected with the vacuum pump. The second opening is connected with the pressure gauge. The three-way air valve is also connected with the reactor. When the three-way air valve is connected with the vacuum pump and the reactor, the vacuum pump can provide a vacuum environment for the reactor and the second glass tube, discharge oxygen in the second glass tube, and remove oxygen dissolved in the reaction solution by using Henry's law to provide an anaerobic or oxygen-free environment for microorganisms.
[0015] In the embodiment of the utility model, the reactor is further provided with a hose valve, the reactor is connected with the three-way air exchange valve through the hose valve, and the hose valve is used for controlling the connection of the reactor and external devices. The reactor is further provided with a reference electrode, a working electrode and a counter electrode, and the reference electrode, the working electrode and the counter electrode are all in contact with the microbial reaction solution in the reactor. The reference electrode, the working electrode and the counter electrode on the reactor can form two loops through the connection of an electrochemical workstation: the working electrode and the counter electrode form a current loop to conduct current, and the working electrode and the reference electrode form a voltage loop, so that the potential of the reference electrode is used to measure the potential of the working electrode, thereby avoiding the influence of polarization on the potential deviation of the counter electrode compared with a two-electrode system. The embodiment of the utility model further comprises a gas collecting bottle, the gas collecting bottle is provided with a valve, the gas collecting bottle is connected with the vacuum pump through the valve, and the gas collecting bottle is used for collecting the extracted gas and plays a role of a vacuum buffer chamber.
[0016] The working principle of the utility model is as follows:
[0017] After the devices are connected, liquid is added into the test tube, the liquid is used for observing the gas flow condition, and common liquids such as water and oil can be selected. The communicating tube of the connected test tube and the first glass tube is inserted into the liquid, the three-way air exchange valve is used to communicate the reactor and the first glass tube, the valve on the argon bottle and the hose valve on the reactor are opened, argon is introduced into the reaction bottle, and uniform and continuous bubbles can be observed at the test tube after a period of time. The three-way air exchange valve is rotated by 180 DEG to make the three-way air exchange valve communicate the reactor and the second glass tube, and the vacuum pump is opened, and the value of the pressure gauge can be observed to continuously decrease, indicating that the pressure in the pipeline is continuously decreasing. After the value of the pressure gauge no longer changes, the three-way air exchange valve is rotated by 180 DEG, the reactor is communicated with the first glass tube again, and the value of the pressure gauge can be observed to continuously increase, indicating that the pipeline is continuously filled with argon. After the value of the pressure gauge stabilizes, the above operation is repeated twice, and the gas charging and gas extraction processes are repeated three times, so that the oxygen removal of the reactor is considered to be completed. After the oxygen removal is completed, the three-way air exchange valve on the reactor is closed first, then the vacuum pump is closed, the hose valve on the reactor is closed, and the reaction bottle is removed, and at this time, it is considered that the reaction bottle is in an oxygen-free environment. The valve of the argon bottle is closed, and no bubbles appear in the test tube. The gas collecting bottle is used for collecting the extracted gas during the gas extraction process and plays a certain buffering role. After the oxygen removal is completed, the gas collecting bottle is opened for gas release, and the gas exchange is completed.
[0018] The above only describes the preferred embodiments of the utility model and is not used for limiting the utility model. For those skilled in the art, the utility model can have various changes and changes. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the utility model should be included in the protection scope of the utility model.
Claims
1. A deoxygenation device for an anaerobic microbial reactor, characterized in that, The apparatus includes an argon cylinder, a reactor, a test tube, a vacuum pump, a pressure gauge, a first glass tube, a second glass tube, and a three-way valve. One end of the first glass tube is connected to the argon cylinder, and the other end is connected to the test tube. A first opening is provided in the middle section of the first glass tube, and the first opening is connected to the three-way valve. One end of the second glass tube is connected to the three-way valve, and the other end is connected to the vacuum pump. A second opening is provided at the end of the second glass tube connected to the vacuum pump, and the second opening is connected to the pressure gauge. The three-way valve is also connected to the reactor.
2. The deoxygenation device for an anaerobic microbial reactor according to claim 1, characterized in that, The reactor is also equipped with a flexible hose valve, which is connected to the three-way ventilation valve.
3. The deoxygenation device for an anaerobic microbial reactor according to claim 2, characterized in that, The reactor is also equipped with a reference electrode, a working electrode, and a counter electrode, all of which are in contact with the microbial reaction solution in the reactor.
4. The deoxygenation device for an anaerobic microbial reactor according to claim 1, characterized in that, It also includes a gas collecting bottle, which is equipped with a valve and is connected to the vacuum pump through the valve.