Exosome extraction device

Abstract

The utility model relates to the field of sample reagent treatment, and discloses an exosome extraction device which comprises a base, a vertical bracket and a shell connected above the base, the vertical support is fixed above the base, and after the shell is fixedly connected with the base, the vertical support is located in the shell; according to the technical scheme, a horizontal oscillation module and a storage module are fixedly connected to the upper portion of a base; a movement module is fixed on the vertical bracket and is used for driving a pipette; the pipette is fixed right above the horizontal oscillation module; a host and a main control board are fixed on any side of the vertical bracket; and a start button is arranged on the shell. The device is simple and convenient to operate and wide in application range, and can be adapted to exosome extraction of various exosome ovarian cancer detection kits.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of sample reagent processing, more specifically to an exosome extraction device. BACKGROUND

[0002] Exosomes are important mediators of intercellular communication, with a unique lipid bilayer structure. These tiny membrane vesicles are widely present in almost all types of normal cells and tumor cells, derived from intracellular multivesicular body luminal membranes. Exosomes can carry biological active substances such as proteins, metabolites and nucleic acids from their source cells, release these substances into the extracellular space through fusion with cell membranes, and thus transmit information between cells.

[0003] The surface of exosomes is rich in various cell surface membrane proteins, which not only reflect the characteristics of their source cells, but also provide potential biomarkers for early detection, diagnosis and prevention of diseases. In particular for cancer, specific membrane proteins on exosomes can serve as a powerful tool for early diagnosis, helping to detect abnormalities at an early stage of disease development, thus providing the possibility for timely treatment and intervention, with extremely high medical value. SUMMARY

[0004] The utility model aims to provide an exosome extraction device, which is simple to operate, widely applicable, and especially suitable for exosome extraction with the exosome ovarian cancer detection kit developed by Mind Diagnostics.

[0005] The utility model provides the following technical scheme: an exosome extraction device, comprising a base 22, a vertical support 23, and a housing 21 connected above the base 22; the vertical support 23 is fixed above the base 22, and after the housing 21 is fixedly connected with the base 22, the vertical support 23 is located inside the housing 21; a horizontal oscillation module 3 and a storage module are fixedly connected above the base 22; a motion module is fixed on the vertical support 23 for driving a pipette 10, the pipette 10 is movably arranged above the horizontal oscillation module 3 and the storage module; a host computer 13 and a main control board 14 are fixed on any side of the vertical support 23; a start button 18 is arranged on the housing 21.

[0006] As a further improvement of the utility model, the storage module comprises a waste storage area 2, a hole plate fixing seat 4, a reagent storage area 5, and a consumable area 6.

[0007] As a further improvement of the utility model, the motion module comprises a first drive motor 7, a second drive motor 8, and a third drive motor.

[0008] As a further improvement of the present application, the vertical support 23 is fixed with an adapter plate 12 and a power supply 15 on any side.

[0009] As a further improvement of the present application, the reagent storage area 5 comprises a precipitating reagent storage area 51 and a lysing reagent storage area 52.

[0010] As a further improvement of the present application, the shell 21 is provided with a hollow part on the top, and the display screen 17 is embedded and fixed in the hollow part.

[0011] As a further improvement of the present application, the shell 21 is connected with an operation door 20 on the bottom, and the operation door 20 is connected with the shell 21 on the bottom; the vertical support 23 is provided with a horizontal oscillation module 3 and a storage module on one side close to the operation door 20; when the operation door 20 is opened, the horizontal oscillation module 3 and the storage module can be operated.

[0012] As a further improvement of the present application, the shell 21 is provided with an emergency stop button 19 on one side.

[0013] As a further improvement of the present application, the pipette 10 is driven by the movement module, and the pipette 10 can move above the waste storage area 2, the horizontal oscillation module 3 and the hole plate fixing seat 4.

[0014] The exosome extraction device has the advantages of simple operation and wide application range, and can better adapt to the exosome ovarian cancer detection kit [registered name: human exosome CA125, HE4, C5a detection kit (chemiluminescence method), trade name: ] for exosome extraction. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 is a three-dimensional structure schematic view of the exosome extraction device of the present application;

[0016] Figure 2 is a schematic view of the operation door of the exosome extraction device of the present application in an open state;

[0017] Figure 3 is a schematic view of one side of the internal three-dimensional structure of the exosome extraction device of the present application;

[0018] Figure 4 is Figure 3 Figure 3 a schematic view of the other side of the internal three-dimensional structure.

[0019] Reference numerals: 1. Motion module, 2. Waste storage area, 3. Horizontal oscillation module, 4. Well plate holder, 5. Reagent storage area, 6. Consumable area, 7. First drive motor, 8. Second drive motor, 9. Z-axis drive module (integrating the third drive motor and Z-axis drive board), 10. Pipette, 11. Y-axis motor driver, 12. Adapter board, 13. Main unit, 14. Main control board, 15. Power supply, 16. X-axis motor driver, 17. Display screen, 18. Start button, 19. Emergency stop button, 20. Operating door, 21. Outer shell, 22. Base, 23. Vertical support, 51. Precipitation reagent storage area, 52. Liquefaction reagent storage area. Detailed Implementation

[0020] To better understand the usage of this utility model, the exosome extraction device of this utility model will be further described below with reference to the accompanying drawings. Identical components are indicated by the same reference numerals. It should be noted that the terms "front," "rear," "left," "right," "upper," and "lower" used in the following description refer to directions in the accompanying drawings, while the terms "bottom surface," "top surface," "inner," and "outer" refer to directions toward or away from the geometric center of a specific component, respectively.

[0021] like Figures 1-4 As shown, this utility model provides an exosome extraction device, which includes a base 22, a vertical support 23, and a housing 21 connected above the base 22. The vertical support 23 is fixed above the base 22, and after the housing 21 is fixedly connected to the base 22, the vertical support 23 is located inside the housing 21. A horizontal oscillation module 3 and a storage module are fixedly connected above the base 22. The horizontal oscillation module 3 is used to mix the sample and reagents. A motion module is fixed on the vertical support 23 to drive a pipette 10, which is movably disposed above the horizontal oscillation module 3 and the storage module. A main unit 13 and a main control board 14 are fixed on either side of the vertical support 23. A start button 18 is provided on the housing 21.

[0022] Furthermore, the storage module includes a waste storage area 2, a plate holder 4, a reagent storage area 5, and a consumables area 6. The waste storage area 2 is used to collect and store discarded pipette tips; the plate holder 4 is used to temporarily store samples or collect transferred samples; the reagent storage area 5 is used to store precipitation and lysis reagents for exosome extraction; and the consumables area 6 is used to store pipette tips. The pipette 10 is driven by the motion module, allowing it to move above the waste storage area 2, the horizontal oscillation module 3, the plate holder 4, the reagent storage area 5, and the consumables area 6.

[0023] Further, the movement module comprises a first driving motor 7, a second driving motor 8 and a third driving motor, wherein the first driving motor 7 is an X-axis driving motor for driving the transverse displacement of the pipette 10, the second driving motor 8 is a Y-axis driving motor for driving the vertical displacement of the pipette 10, and the third driving motor is a Z-axis driving motor for driving the longitudinal displacement of the pipette 10, wherein the X-axis driving motor 7 is driven to operate by an X-axis motor driver 16, the Y-axis driving motor 8 is driven to operate by a Y-axis motor driver 11, and the Z-axis driving motor and a Z-axis driving plate constitute a Z-axis driving module 9, and the Z-axis driving motor is driven to operate by the Z-axis driving plate.

[0024] Further, the vertical support 23 is fixed with an adapter plate 12 and a power supply 15 on any one side thereof; preferably, the adapter plate 12 and the power supply 15 are fixed on the same side.

[0025] Further, the reagent storage area 5 comprises a precipitation reagent storage area 51 and a lysis reagent storage area 52, wherein the precipitation reagent storage area 51 is used for storing 5ml reagent tubes containing precipitation reagents for exosome extraction, and the lysis reagent storage area 52 is used for storing 10ml reagent tubes containing lysis reagents for exosome extraction.

[0026] Further, a hollow part is arranged above the shell 21, and a display screen 17 is embedded and fixed in the hollow part; preferably, the display screen 17 is a touchable display screen 17.

[0027] Further, a hollow part is also arranged below the shell 21, and the hollow part corresponds to the horizontal oscillation module 3 and the storage module inside the device; an operation door 20 capable of being opened downward is arranged in the hollow part below the shell 21, and the operation door 20 is connected to the shell 21 in a hinged manner; the operation door 20 is connected to the shell 21 at the bottom; the horizontal oscillation module 3 and the storage module are arranged on the side of the vertical support 23 close to the operation door 20; when the operation door 20 is opened, the horizontal oscillation module 3 and the storage module can be operated from the outside.

[0028] Further, an emergency stop button 19 is arranged on one side of the shell 21, which is used for emergency shutdown of the device in case of emergency.

[0029] The serum sample processing process is carried out by the exosome extraction device.

[0030] 1. The filter hole plate is arranged on the 48-hole plate, and the serum sample is collected by the 48-hole plate after being filtered by the filter hole plate.

[0031] 2. After the instrument is initialized, follow the on-screen prompts to load the following: (1) Load the filtered sample into the sample holder 4; (2) Place a new 48-well plate into the horizontal shaking module; (3) Load the precipitation reagent into the precipitation reagent storage area 51 of the reagent storage area 5; (4) Load the pipette tips into the consumable area 6 and confirm the number and location of the pipette tips; (5) Load the trash (empty tip box) into the waste storage area 2 and confirm the number and location of the empty tip locations.

[0032] 3. The instrument performs the "Exosome Precipitation Reagent Addition and Mixing" process, which includes the following steps: ① Sample transfer (use the pipette tips to transfer the sample to the empty 48-well plate in the horizontal shaking module); ② Add the precipitation reagent (use the pipette tips to add the precipitation reagent to the 48-well plate in the horizontal shaking module and mix with the sample); ③ Mix by pipetting (use the pipette tips to mix the sample and reagent by pipetting); ④ Mix by shaking (shake the horizontal shaking module to further mix); and ⑤ Incubate.

[0033] 4. After the "Exosome Precipitation Reagent Addition and Mixing" process is complete, follow the on-screen prompts to remove the 48-well plate from the horizontal shaking module and the sample holder 4, and manually process the subsequent process (centrifugation to remove the supernatant) to obtain the exosome precipitate.

[0034] 5. After the exosome precipitate is obtained, follow the on-screen prompts to load the following: (1) Load the lysis reagent into the lysis reagent storage area 52 of the reagent storage area 5; (2) Load the sample for the second time (load the exosome precipitate) into the horizontal shaking module; (3) Load the 48-well plate with the filter plate: place the empty 48-well plate with the filter plate into the sample holder 4 of the instrument; (4) Load the pipette tips into the consumable area 6 and confirm the number and location of the pipette tips; (5) Load the trash (empty tip box) into the waste storage area 2 and confirm the number and location of the empty tip locations.

[0035] 6. The instrument performs the "Exosome Lysis Reagent Addition and Mixing" process, which includes the following steps: ① Add the reagent: use the pipette tips to add the exosome lysis reagent to the 48-well plate with the sample in the horizontal shaking module; ② Mix by shaking: shake the horizontal shaking module to mix the exosome precipitate and the lysis reagent; ③ Transfer the lysis product: use the pipette tips to transfer the product to the filter plate in the empty 48-well plate in the sample holder 4, at which point the product is retained in the filter plate and does not completely enter the 48-well plate.

[0036] 7. Remove the 48-well plate and the filter plate from the sample holder 4, and centrifuge to collect the filtered exosome product in the 48-well plate.

[0037] 8. The obtained exosome product can be used for subsequent clinical in vitro detection, such as chemiluminescence detection.

[0038] The above are only preferred embodiments of the present application, and the protection scope of the present application is not limited to the above-mentioned embodiments, and any technical scheme falling within the concept of the present application belongs to the protection scope of the present application. It should be noted that, for ordinary skilled persons in the art, some improvements and refinements without departing from the principles of the present application are also considered to be within the protection scope of the present application.

Claims

1. An exosome extraction device, comprising a base, a vertical support and a shell connected above the base; the vertical support is fixed above the base, and the shell is fixedly connected with the base, and the vertical support is located inside the shell; characterized in that, The base is fixedly connected with a horizontal oscillation module and a storage module above; the vertical support is fixed with a movement module for driving a pipette, and the pipette is movably arranged above the horizontal oscillation module and the storage module.

2. The exosome extraction device of claim 1, wherein, The storage module comprises a waste storage area, a hole plate fixing seat, a reagent storage area and a consumable area.

3. The exosome extraction device of claim 1, wherein, The movement module comprises a first driving motor, a second driving motor and a third driving motor.

4. The exosome extraction device of claim 1, wherein, The vertical support is fixed with an adapter plate and a power supply on any side.

5. The exosome extraction device of claim 2, wherein, The reagent storage area comprises a precipitation reagent storage area and a lysis reagent storage area.

6. The exosome extraction device of claim 1, wherein, The shell is provided with a hollow part above, and a display screen is embedded and fixed in the hollow part.

7. The exosome extraction device of claim 1, wherein, The shell is connected with an operation door below, and the operation door is connected with the shell by the bottom; the horizontal oscillation module and the storage module are arranged on the side of the vertical support close to the operation door; when the operation door is opened, the horizontal oscillation module and the storage module can be operated from the outside.

8. The exosome extraction device of claim 1, wherein, An emergency stop button is arranged on one side of the shell.

9. The exosome extraction device of claim 2, wherein, The pipette is driven by the movement module, and the pipette moves above the waste storage area, the horizontal oscillation module and the hole plate fixing seat.

10. The exosome extraction device of claim 1, wherein, The vertical support is fixed with a main machine and a main control board on any side; and the shell is provided with a start button.

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