Automatic replacement device for cell culture fluid

Abstract

The utility model provides an automatic replacement device for cell culture fluid, and belongs to the technical field of cell culture. Comprising a bottom plate; the culture tank is arranged in the center of the top of the bottom plate; the two collecting tanks are arranged at one end of the culture tank; the recycling assembly is arranged between the culture tank and the collection tank and used for collecting the old culture solution, and the recycling assembly comprises a recycling pump arranged on the bottom plate; one end of the first recovery pipe is communicated with the culture tank, and the other end of the first recovery pipe is communicated with one end of the recovery pump. According to the utility model, the filter is arranged to filter the old culture solution, the old culture solution is checked through the monitor, the old culture solution is divided into two parts according to the detection result, the old culture solution is discharged into the corresponding collection pipes through the two second recovery pipes, and the old culture solution is distinguished and collected, so that the subsequent treatment is facilitated.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field, especially a kind of cell culture medium automatic replacement device. BACKGROUND

[0002] Cell culture refers to simulating in-vivo environment in vitro, sterile, suitable temperature, pH and certain nutrient conditions, etc., so that cell survival, growth, reproduction and maintain the main structure and function of a kind of technology. Cell in growth and metabolism process, will continue to consume the nutrient substance in culture medium, with the extension of culture time, these nutrient substances gradually reduce, when reduce to certain degree, will not meet the needs of cell growth, need to replace culture medium, therefore need to use replacement device.

[0003] At present, the existing replacement device after replacing culture medium, old culture medium is usually discarded directly, wherein the nutrient substance not completely consumed and other resources are wasted, at the same time, waste liquid can contain harmful substances produced by cell metabolism, if not treated directly discharges, also can cause pollution to environment, therefore, the present application provides a kind of cell culture medium automatic replacement device to meet the needs. UTILITARIAN CONTENT

[0004] The technical problem to be solved by the utility model is to provide a kind of cell culture medium automatic replacement device to solve the problem that the existing replacement device cannot be differentiated according to different degrees of old culture medium after replacing culture medium.

[0005] To solve the above technical problems, the utility model provides the following technical scheme:

[0006] A kind of cell culture medium automatic replacement device, comprising: bottom plate;Culture tank, is arranged at the center of the top of the bottom plate;Two collection tanks, are arranged at one end of the culture tank;Recycling assembly, is arranged between the culture tank and the collection tank, for collecting old culture medium, the recycling assembly includes: recycling pump, is arranged on the bottom plate;First recovery pipe, one end is communicated with the culture tank, the other end is communicated with one end of the recycling pump;Monitor, is communicated and arranged in the other end of the recycling pump, for detecting old culture medium;Two second recovery pipes, one end is communicated with the monitor, the other end is communicated with the collection tank.

[0007] Preferably, filter, is arranged on the first recovery pipe, for filtering old culture medium.

[0008] Preferably, sensor, is arranged on the culture tank, for detecting the culture tank liquid level height and cell growth degree in culture tank;Replacement pump, is arranged on the bottom plate, and is electrically connected with the sensor.

[0009] Preferably, a storage tank is arranged at the other end of the culture tank; a first connecting pipe is in communication with the storage tank at one end and with the replacement pump at the other end.

[0010] Preferably, a second connecting pipe is in communication with the replacement pump at one end and with the culture tank at the other end.

[0011] Preferably, an outer cylinder is sleeved on the surface of the first connecting pipe; the first connecting pipe is transparent at the sleeving area of the outer cylinder.

[0012] Preferably, an ultraviolet lamp is arranged between the outer cylinder and the first connecting pipe in a spiral shape.

[0013] Compared with the prior art, the present application has at least the following beneficial effects:

[0014] In the above scheme, the old culture solution is filtered, the old culture solution is checked by the monitor, and the old culture solution is divided into two parts according to the detection result, and the old culture solution is discharged into the corresponding collection pipe through the two second recovery pipes, so that the old culture solution is collected and treated.

[0015] The ultraviolet lamp is arranged to irradiate the liquid in the pipeline in all directions and without dead angle, to create a cleaner liquid transmission environment for cell culture and to ensure that the cell culture process is not contaminated. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is a structure schematic view of the cell culture solution automatic replacement device.

[0017] Figure 2 It is a structure schematic view of the ultraviolet lamp.

[0018] Figure 3 It is a structure schematic view of the recovery assembly.

[0019] In the figure: 1, bottom plate; 2, replacement pump; 3, sensor; 4, collection tank; 5, recovery assembly; 6, culture tank; 7, second connecting pipe; 8, storage tank; 9, first connecting pipe; 10, outer cylinder; 11, ultraviolet lamp; 51, second recovery pipe; 52, monitor; 53, recovery pump; 54, filter; 55, first recovery pipe.

[0020] As shown in the figure, in order to clearly realize the structure of the embodiment of the present application, specific structures and devices are marked in the figure, but this is only for illustration and is not intended to limit the present application to this specific structure, device and environment. According to specific needs, those skilled in the art can adjust or modify these devices and environments, and the adjustments or modifications still include in the scope of the appended claims. DETAILED DESCRIPTION

[0021] The automatic cell culture medium replacement device provided by this utility model will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that, to make the embodiments more detailed, the following embodiments are the best and preferred embodiments. For some known technologies, those skilled in the art can also use other alternative methods to implement them; moreover, the accompanying drawings are only for more specific description of the embodiments and are not intended to specifically limit this utility model.

[0022] like Figure 1 - Figure 3 As shown, an embodiment of this utility model provides an automatic cell culture medium replacement device, comprising: a base plate 1; a culture tank 6 disposed at the center of the top of the base plate 1; two collection tanks 4 disposed at one end of the culture tank 6; and a recovery assembly 5 disposed between the culture tank 6 and the collection tanks 4 for collecting old culture medium. The recovery assembly 5 includes: a recovery pump 53 disposed on the base plate 1; a first recovery pipe 55, one end of which is connected to the culture tank 6 and the other end of which is connected to one end of the recovery pump 53; a monitor 52 disposed at the other end of the recovery pump 53 for detecting old culture medium; and two second recovery pipes 51, one end of which is connected to the monitor 52 and the other end of which is connected to the collection tanks 4.

[0023] All pipelines in the replacement device are connected using aseptic technology to ensure that the cell culture environment is not contaminated. Monitors 52 are installed to monitor both the composition and physical properties. The composition analysis includes spectral or chromatographic analysis, while the physical properties include turbidity and particulate matter or conductivity.

[0024] Filter 54, located on the first recovery tube 55, is used to filter old culture medium. Filter 54 typically uses a microporous membrane, the pore size of which is selected according to the size of the impurities to be filtered. It can usually effectively filter out cell debris, microorganisms, and other tiny particles. The outer shell of filter 54 is made of corrosion-resistant plastic.

[0025] Sensor 3, installed on culture tank 6, is used to detect the level of culture medium and the degree of cell growth within culture tank 6. Replacement pump 2, installed on base plate 1, is electrically connected to sensor 3. By installing sensor 3, after detecting that the level of culture medium in culture tank 6 has dropped to a dangerous range, the replacement pump 2 is controlled to operate, replenishing culture medium in culture tank 6; at the same time, sensor 3 can control the degree of culture medium replacement according to the degree of cell growth.

[0026] Storage tank 8 is located at the other end of culture tank 6; first connecting pipe 9 is connected to storage tank 8 at one end and to replacement pump 2 at the other end. A control valve is installed at the end of first connecting pipe 9 near storage tank 8, and the control valve is electrically connected to sensor 3.

[0027] The second connecting pipe 7 is communicated with the replacing pump 2 at one end and communicated with the culture tank 6 at the other end.

[0028] The outer cylinder 10 is sleeved on the surface of the first connecting pipe 9, and the sleeving area of the first connecting pipe 9 by the outer cylinder 10 is transparent. The outer cylinder 10 can protect the ultraviolet lamp 11, and is detachably installed, so that the ultraviolet lamp 11 can be replaced or maintained later.

[0029] The ultraviolet lamp 11 is spirally arranged between the outer cylinder 10 and the first connecting pipe 9. The ultraviolet lamp 11 can sterilize and disinfect the culture solution flowing therethrough, and the spiral arrangement increases the irradiation area.

[0030] When the culture tank 6 needs to replace the culture solution, the recycling pump 53 operates, the old culture solution in the culture tank 6 is drawn out through the first recycling pipe 55, and the old culture solution is filtered through the filter 54. The old culture solution flows into the monitor 52, and the monitor 52 comprehensively detects the composition and physical properties of the old culture solution. According to the detection result, the old culture solution is discharged into the corresponding collecting tank 4 through the two second recycling pipes 51. After the waste liquid is recycled, the replacing pump 2 operates, the culture solution in the storage tank 8 is drawn out through the first connecting pipe 9, and the culture solution is irradiated and disinfected by the ultraviolet lamp 11 when flowing into the transparent area of the first connecting pipe 9. Then, the culture solution is injected into the culture tank 6 through the second connecting pipe 7.

[0031] The utility model covers any alternative, modification, equivalent method and scheme which are made on the essence and range of the utility model. In order to make the public have the thorough understanding of the utility model, the specific details are explained in the above preferred embodiment of the utility model, and the utility model can be completely understood without the description of these details for the person skilled in the art. In addition, in order to avoid unnecessary confusion to the essence of the utility model, the well-known method, process, flow, element and circuit are not explained in detail.

[0032] The above is only the preferred embodiment of the utility model, and it should be pointed out that for ordinary skilled in the art, without departing from the principle of the utility model, a plurality of improvements and refinements can be made, and these improvements and refinements should be regarded as the protection range of the utility model.

Claims

1. An automatic cell culture medium replacement device, characterized in that, include: Base plate (1); The culture tank (6) is located at the center of the top of the bottom plate (1); Two collection tanks (4) are disposed at one end of the culture tank (6); A recovery assembly (5), disposed between the culture tank (6) and the collection tank (4), is used to collect old culture medium. The recovery assembly (5) includes: A recovery pump (53) is mounted on the base plate (1); The first recovery pipe (55) is connected at one end to the culture tank (6) and at the other end to one end of the recovery pump (53); A monitor (52), connected to the other end of the recovery pump (53), is used to detect old culture medium; Two second recovery tubes (51) are connected at one end to the monitor (52) and at the other end to the collection tank (4).

2. The automatic cell culture medium replacement device according to claim 1, characterized in that, Also includes: A filter (54) is provided on the first recovery tube (55) for filtering old culture medium.

3. The automatic cell culture medium replacement device according to claim 1, characterized in that, Also includes: Sensor (3) is installed on the culture tank (6) to detect the liquid level of the culture medium and the degree of cell growth in the culture tank (6); Replace the pump (2), set it on the base plate (1), and electrically connect it to the sensor (3).

4. The automatic cell culture medium replacement device according to claim 3, characterized in that, Also includes: A storage tank (8) is located at the other end of the culture tank (6); The first connecting pipe (9) is connected at one end to the storage tank (8) and at the other end to the replacement pump (2).

5. The automatic cell culture medium replacement device according to claim 3, characterized in that, Also includes: The second connecting pipe (7) is connected at one end to the replacement pump (2) and at the other end to the culture tank (6).

6. The automatic cell culture medium replacement device according to claim 4, characterized in that, Also includes: The outer cylinder (10) is fitted onto the surface of the first connecting pipe (9); The area of ​​the first connecting pipe (9) covered by the outer cylinder (10) is transparent.

7. The automatic cell culture medium replacement device according to claim 6, characterized in that, Also includes: An ultraviolet lamp (11) is arranged in a spiral shape between the outer cylinder (10) and the first connecting pipe (9).

Images