Sequencing and analysis integrated device

Abstract

The utility model provides a sequencing and analysis integrated device, and relates to the field of biomolecule detection.The device comprises a sequencing mechanism and an analysis mechanism electrically connected with the sequencing mechanism and further comprises a pretreatment mechanism, and the pretreatment mechanism is electrically connected with the sequencing mechanism and the analysis mechanism; after the pretreatment mechanism treats a sample solution, the sequencing mechanism receives the treated sample; the pretreatment mechanism comprises a micro-fluidic chip, and an electrode plate matrix is arranged in the micro-fluidic chip; the device further comprises a protection mechanism, and the sequencing mechanism, the analysis mechanism and the pretreatment mechanism are arranged in the protection mechanism. The device integrates a pretreatment mechanism, a sequencing mechanism and an analysis mechanism, real-time sequencing under a non-laboratory scene can be realized on one instrument, the integrated design enables the device to have complete functions, the whole-process treatment of sequencing operation can be solved in a one-stop manner, and the device is convenient to move.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biological molecule detection, especially to a sequencing analysis integrated device. BACKGROUND

[0002] Nanopore sequencing technology is the third generation sequencing technology, which uses nanopore as a biosensor to identify and measure the sequence of single DNA and RNA molecules. The working principle is that the DNA or RNA molecule passes through the nanopore under the control of the motor protein. Since the current changes caused by different bases passing through the nanopore are different, different bases are determined by monitoring the current signal to achieve sequencing. Compared with previous sequencing technologies, nanopore sequencing can provide long read sequencing, and can monitor the sequencing results in real time according to the current change when a single molecule passes through the pore.

[0003] Before nanopore sequencing, nucleic acid extraction, sequencing library construction and other pretreatment processes are needed. Sequencing library construction refers to a series of processes performed on the original DNA or RNA sample before next-generation sequencing to convert it into a format suitable for sequencing instrument reading. The process usually includes fragmentation, end repair, adapter addition, PCR (Polymerase Chain Reaction) amplification and other steps. In traditional technology, the above-mentioned nucleic acid extraction and sequencing library construction and other experimental steps are complex and time-consuming, and require high standardization of experimental personnel operation. And the current nanopore sequencing pretreatment, sequencing and sequencing result analysis three workflows are relatively independent and separated. In order to realize instant sequencing at the border port or other non-laboratory scenes, rapid detection of pathogens and other pathogens is needed, and a full-process integrated and portable sequencing device is needed.

[0004] There are instruments in the prior art only for sequencing pretreatment, which can be better applied to nanopore sequencing technology to replace manual operation and simplify the operation process. On the other hand, for example, the Chinese invention patent with publication number CN115948235A discloses a "DNA sequencing analysis integrated device based on solid-state nanopore", which integrates nanopore sequencing acquisition technology, membrane clamp control technology, software control technology, 5G communication processing unit and operation display terminal into the same device, which can complete the complete process of DNA nanopore sequencing operation, including pore event recognition, single and double strand detection, acquisition of electrical signals, data file packaging, transmission and reception of data analysis, and DNA sequencing sequence. The device realizes the acquisition, transmission and analysis of data obtained by sampling and sequencing in the field by a portable "terminal" device. However, the device only solves the problem of relative independence of current sequencing and sequencing result analysis, and the nanopore sequencing pretreatment process still relies on the existing independent processing method, which needs to be further improved. UTILITY MODEL CONTENT

[0005] The utility model discare the defects in the prior art, and an integrated sequencing and analysis device is provided, which integrates a pretreatment mechanism, a sequencing mechanism and an analysis mechanism, can solve the whole process of sequencing operation in one station, and therefore can overcome the long sequencing time caused by independent separation of individual processes.

[0006] In order to achieve the above object, the utility model adopts the following technical scheme:

[0007] The utility model provides a sequencing and analysis integrated device, including sequencing mechanism and with the sequencing mechanism electricity connection's analysis mechanism, still include pretreatment mechanism, pretreatment mechanism with sequencing mechanism, analysis mechanism electricity connection, pretreatment mechanism handles sample solution, and sequencing mechanism receives the sample after processing, pretreatment mechanism includes micro -fluidic chip, and the electrode piece matrix is arranged in micro -fluidic chip, still include protection mechanism, and sequencing mechanism, analysis mechanism and pretreatment mechanism set up in protection mechanism.

[0008] The pretreatment mechanism is a sequencing pretreatment device for nucleic acid extraction and sequencing library construction. The micro-fluidic chip is a biological molecule detection chip with micro-fluid channels, which can perform nucleic acid extraction and other steps. Compared with manual operation, it has the advantages of short time and high precision, can solve the problem of high standardization requirement of operators in the sequencing pretreatment step, and simplifies the operation process. The electrodes in the micro-fluidic chip are arranged in columns or rows to form an electrode matrix. Using micro-droplet control technology and dielectric wetting effect, the pathogen samples and related reagents to be detected move, rotate or gather in all directions in the micro-fluidic chip, realizing rapid and accurate control of droplets, and completing DNA end repair and other operations. The sequencing mechanism is a sequencer that can use nanopore sequencing technology. The principle is to determine the sequence of bases passing through the nanopore by the slight change of current when DNA or RNA molecules pass through the nanopore, so as to determine the sequence of molecules. The analysis mechanism collects and analyzes the data of molecular pretreatment and sequencing, and finally obtains the analysis result through the connected display. The integrated design of the device presents the three main links in gene sequencing in one instrument, and can perform the whole process of sequencing in sequence, thereby reducing the time required for material transportation and data transmission between each link.

[0009] Further, the sequencing mechanism is provided with a sequencing flow channel, the sequencing flow channel is provided with a nanopore, and the sequencing flow channel has a liquid inlet and a liquid outlet, wherein the liquid inlet is provided with a sealing ring.

[0010] The sample solution is reacted in the sequencing flow channel, and the nucleotide completes the determination of the base thereon through the nanopore in the sequencing flow channel. When the sample solution is poured through the pipette, the sealing ring can tightly abut the liquid outlet of the pipette, effectively preventing sample leakage and preventing biochemical transmission of viruses and the like, thereby ensuring personnel safety.

[0011] Further, the analysis mechanism comprises a processor and a chip data board electrically connected with the processor, and the processor is provided with a support seat at the bottom; the support seat has a base plate and a base, the processor is installed on the base plate, and the chip data board is installed on the base.

[0012] The support seat is a double-layer upper and lower structure, the base plate is at the upper layer and is supported by the base. The processor is installed on the base plate at the upper layer, and the chip data board is fixed in the middle layer between the base plate and the base and is installed in the protection mechanism together with the support seat. The processor is the collection and analysis instrument of the device, is provided with an analysis software, needs to be connected with external equipment such as a display and an input device for use, can collect main data of sequencing, such as operation data of the sequencing mechanism and perform data analysis, and the chip data board is the data collection center of the microfluidic chip and is used for data analysis of the microfluidic chip.

[0013] Further, the sequencing mechanism is provided with a signal amplification unit, which amplifies the current signal when the nanopore forms a micro-current.

[0014] The signal amplification unit is a signal amplification circuit arranged in the total circuit of the sequencing mechanism, can accurately capture the slight current change of the nanopore, amplifies the current signal, and is beneficial to parameter analysis of the analysis mechanism and improves sequencing accuracy.

[0015] Further, a single-chip microcomputer is fixed on the chip data board and is electrically connected with the processor.

[0016] The single-chip microcomputer serves as the controller of the chip data board, can further collect and analyze the operation result of the microfluidic chip, and can be connected with an indicator lamp and a radiator and the like, thereby improving the use effect of the device.

[0017] Further, a socket is arranged on the protection mechanism and is clamped with the pretreatment mechanism.

[0018] The socket is the mounting position of the pretreatment mechanism, the pretreatment mechanism can be disassembled and mounted at any time through clamping with the socket, so that replacement and assembly are facilitated, and in addition, the microfluidic chip can be transported separately from the device main body during transportation, so that the microfluidic chip is prevented from being accidentally damaged in the middle of the way, thereby reducing loss. The device main body is the protection mechanism and various components therein, and is the remaining part except the sequencing mechanism and the pretreatment mechanism.

[0019] Further, the protection mechanism is provided with a mounting groove, and the sequencing mechanism is embedded in the mounting groove.

[0020] The mounting groove is located at the top of the protection mechanism and matches the sequencing mechanism, and is used for limiting the sequencing mechanism.

[0021] The specific implementation process is as follows: the operator carries the device to the relevant place, connects the power supply to the device body before use, then installs the sequencing mechanism and the micro-fluidic chip on the device body, and connects each component through the wire and connects with external equipment such as a touch screen. After the power is turned on, the operator can use the pipette to move the prepared pathogen sample solution into the micro-fluidic chip, and collect the running data of the micro-fluidic chip through the touch screen using the analysis software. After the touch screen displays that the pretreatment is completed, the pipette is used again to move the pathogen sample into the sequencing mechanism. The sequencing result analysis after this can be conveniently operated through the touch screen, and finally the detection result of the above-mentioned pathogen sample is obtained, and the instant sequencing in different scenes is realized.

[0022] The beneficial effects of the utility model at least include:

[0023] The utility model provides a sequencing analysis integrated device, integrated pretreatment mechanism, sequencing mechanism and analysis mechanism, through analysis mechanism to pretreatment mechanism and sequencing mechanism data information collection, realize the control of sequencing process, through analysis mechanism to sequencing data processing can realize sequencing result analysis, so as to realize instant sequencing in non-laboratory scene on a instrument. The pretreatment mechanism adopts micro-fluidic chip, and the movement, rotation or aggregation of the pathogen sample to be detected and related reagents are realized by using electrode sheet matrix, accurate control droplet, improve efficiency. Integrated design makes the device complete functions, can one-stop solution sequencing operation whole process processing, and then solves the problem that sequencing whole process is relatively independent in application, therefore, the defect that sequencing time is long due to individual process independent separation can be overcome, and the device is convenient to move. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a three-dimensional schematic view of the sequencing analysis integrated device provided by the application Figure 1 ;

[0025] Figure 2 It is a three-dimensional schematic view of the sequencing analysis integrated device provided by the application Figure 2 ;

[0026] Figure 3 It is an internal structure schematic view of the protection mechanism provided by the application

[0027] Figure 4 is a structural schematic diagram of an analysis mechanism provided by the present application;

[0028] Figure 5 is a frame structure schematic diagram of a sequencing analysis integrated device according to Embodiment 2 of the present application.

[0029] Reference signs:

[0030] 1. a sequencing mechanism;

[0031] 2. an analysis mechanism; 21. a processor; 22. a chip data board;

[0032] 3. a pretreatment mechanism; 31. a microfluidic chip;

[0033] 4. a protection mechanism; 41. a socket; 42. a mounting groove;

[0034] 5. a support seat; 51. a base plate; 52. a base. DETAILED DESCRIPTION

[0035] The preferred embodiments of the present application will be described in more detail by referring to the attached drawings. Although the preferred embodiments of the present application are shown in the drawings, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to make the present application more thorough and complete, and to fully convey the scope of the present application to those skilled in the art.

[0036] In the description of the present application, the terms "upper", "lower", "left" and "right" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and do not require the present application to be constructed and operated in a particular orientation, therefore it cannot be understood as a limitation of the present application.

[0037] The terms used in the present application are only for the purpose of describing specific embodiments and are not intended to limit the present application. In the present application and the appended claims, the singular forms "a", "an" and "the" are also intended to include the plural forms, unless the context clearly indicates otherwise. It should also be understood that the term "and / or" used herein means and includes any or all possible combinations of one or more associated listed items.

[0038] Embodiment 1

[0039] Referring to Figures 1 to 4As shown, the utility model provides a sequencing analysis integrated device, including sequencing mechanism 1 and with sequencing mechanism 1 electric connection's analysis mechanism 2, still including preprocessing mechanism 3, preprocessing mechanism 3 with sequencing mechanism 1, analysis mechanism 2 electric connection, preprocessing mechanism 3 handles sample solution after, sequencing mechanism 1 receives the sample after processing, preprocessing mechanism 3 includes micro -fluidic chip 31, and electrode piece matrix is arranged in micro -fluidic chip 31. Still including protection mechanism 4, sequencing mechanism 1, analysis mechanism 2 and preprocessing mechanism 3 are arranged in protection mechanism 4.

[0040] Specifically, preprocessing mechanism 3 is sequencing preprocessing device, is used for nucleic acid extraction and sequencing library construction and so on preprocessing process. And micro -fluidic chip 31 can carry out nucleic acid extraction and so on step, compared with manual operation, with the advantages of short time, high precision, can solve the problem that sequencing preprocessing step requires high standardization of operator, simplifies the operation process. Micro -fluidic chip 31 is connected with analysis mechanism 2 by wire to transmit data signal. The electrode piece in micro -fluidic chip 31 is arranged into column or row, constitutes electrode piece matrix, through this electrode piece matrix using micro droplet control technology, so that the pathogen sample to be detected and related reagent move, rotate or gather in each direction in micro -fluidic chip 31, realize the rapid and accurate control of droplet, and then complete DNA end repair and so on operation. Among them, micro droplet control technology applies dielectric wetting effect, and dielectric wetting effect refers to when small droplet contacts solid surface, under the action of electric field formed on medium surface, droplet surface charge distribution is uneven, shows a kind of wetting phenomenon. Therefore, electrode piece matrix can make droplet surface unbalanced force by adjusting electric field intensity and frequency and so on parameter mode, and then move, rotate or gather in each direction. Sequencing mechanism 1 is existing sequencing machine, specifically can adopt commercial or scientific research nanometer hole sequencing machine, by using nanometer hole sequencing technology, the order of base passing is determined by the small change of electric current in nanometer hole when passing through DNA or RNA molecule, so as to determine the order of molecular arrangement, and the electric signal of sequencing can be transmitted. Analysis mechanism 2 collects the electric signal transmitted by nanometer hole sequencing machine simultaneously to carry out data analysis, and finally obtains analysis result through connecting display.

[0041] The protection mechanism 4 can be various housings for ensuring safe operation and transportation safety of each mechanism, and can centrally install and package the sequencing mechanism 1, the analysis mechanism 2, and the pretreatment mechanism 3, and can also install the display and other peripherals on the housing to improve portability. The housing can be designed as a hand-held, push-pull, or detachable fixed type according to the carrying mode to meet different mobile needs. This design reduces space occupation and provides a more convenient choice for the movement and transportation of the device, so that it can be flexibly transferred in various scenes. The integrated design of the device enables the three main links in gene sequencing to be presented in one instrument, and the full-process processing of sequencing can be performed in sequence, reducing the time required for material transportation and data transmission between each link.

[0042] In the embodiment, the sequencing mechanism 1 is provided with a sequencing flow channel, the sequencing flow channel is provided with a nanopore, and the sequencing flow channel has a liquid inlet and a liquid outlet, and a sealing ring is arranged at the liquid inlet.

[0043] Specifically, the sample solution is reacted in the sequencing flow channel, and the nucleotide completes the determination of the base thereon by passing through the nanopore in the sequencing flow channel. When the sample solution is poured by the pipette, the sealing ring can tightly abut the liquid outlet of the pipette, effectively preventing sample leakage and preventing biochemical transmission of viruses and the like, thereby ensuring personnel safety.

[0044] The sequencing mechanism 1 in the embodiment is also provided with a signal amplification unit, which amplifies the current signal when the nanopore forms a micro-current.

[0045] Specifically, the signal amplification unit is a signal amplification circuit provided in the total circuit of the sequencing mechanism 1, which can accurately capture the slight current change of the nanopore and amplify the current signal, which is conducive to parameter analysis by the analysis mechanism 2 and improves the sequencing accuracy.

[0046] Further, the microfluidic chip 31 is provided with a heating resistor and a thermistor, the solution in the microfluidic chip 31 is heated by the heating resistor, and the temperature is controlled by the thermistor, so that the reaction temperature in the solution is stable, which is conducive to achieving the temperature required for reagent reaction in the microfluidic chip 31, thereby accelerating the sequencing pretreatment.

[0047] Referring to Figure 4 In the embodiment, the analysis mechanism 2 includes an embedded processor 21 and a circuit board, the circuit board is attached to the bottom of the protection mechanism 4, and the embedded processor 21 is installed on the circuit board and electrically connected to the circuit board.

[0048] It should be noted that the embedded processor 21 can adopt an embedded module with a model of jetson orin nano, wherein a preset analysis software programmed in a python language is built-in, which can collect relevant data information of the operation and start-stop of the microfluidic chip 31 and the sequencing mechanism 1, obtain sequencing data in the sequencing mechanism 1 after being connected to a power supply, and perform analysis when the data collection is completed. When the data analysis is performed, the embedded processor 21 compares the downloaded database in the analysis software with the sequencing data, thereby confirming molecular information to obtain an analysis result. The circuit board can provide a power supply for the embedded processor 21, and a plurality of interfaces such as a USB interface are arranged on the circuit board, so that the embedded processor 21 can be connected to other components such as the sequencing machine, the microfluidic chip 31 and the touch device through the circuit board.

[0049] In the embodiment, a power management system is further included, and the power management system includes an AC 220V to DC 19V transformer and a 19V to 50-500V adjustable transformer.

[0050] Specifically, the AC 220V to DC 19V transformer is connected to a commercial power supply for supplying power to the embedded processor 21, and the 19V to 50-500V adjustable transformer is electrically connected to the AC 220V to DC 19V transformer through a wire for supplying power to the electrode sheet matrix on the microfluidic chip 31, so that the electrode sheet matrix drives droplets by adjusting relevant parameters in an electric field or controlling the on-off sequence of high-voltage current through a dielectric wetting principle, to realize reagent movement.

[0051] Embodiment 2

[0052] Referring to Figures 1 to 4 As shown in the figure, the embodiment provides a sequencing and analysis integrated device, which includes a sequencing mechanism 1 and an analysis mechanism 2 electrically connected to the sequencing mechanism 1; further includes a pretreatment mechanism 3 electrically connected to the sequencing mechanism 1 and the analysis mechanism 2; further includes a protection mechanism 4, and the sequencing mechanism 1, the analysis mechanism 2 and the pretreatment mechanism 3 are arranged in the protection mechanism 4.

[0053] Specifically, the preprocessing mechanism 3 is a sequencing pre-processing device, which can specifically adopt an existing sequencing pre-processing instrument, and is used for nucleic acid extraction and sequencing library construction and other preprocessing processes. The sequencing mechanism 1 is an existing sequencing machine, which can specifically adopt a commercial or scientific nanopore sequencing machine. The nanopore sequencing technology is used to determine the sequence of bases passing through the nanopore by the slight change of the current when the DNA or RNA molecule passes through the nanopore, so as to determine the sequence of the molecule, and the electrical signal of the sequencing can be transmitted. The analysis mechanism 2 simultaneously collects the electrical signal transmitted by the nanopore sequencing machine for data analysis, and finally obtains the analysis result by connecting the display. The integrated design of the device makes the three main links in the genetic sequencing appear in one instrument, and the whole process of sequencing can be processed by sequential operation, which shortens the time required for the connection between each link for material transportation and data transmission.

[0054] In the embodiment, the preprocessing mechanism 3 includes a microfluidic chip 31, and the microfluidic chip 31 is provided with an electrode sheet matrix.

[0055] Specifically, the microfluidic chip 31 can perform nucleic acid extraction and other steps, which has the advantages of short time and high precision compared with manual operation, can solve the problem of high standardization requirement of the operator in the pre-processing step of sequencing, and simplifies the operation process. The electrode sheets in the microfluidic chip 31 are arranged in columns or rows to form an electrode sheet matrix. Through the electrode sheet matrix, the microdroplet manipulation technology is used to make the pathogen samples to be detected and related reagents move, rotate or gather in various directions in the microfluidic chip 31, realize the rapid and accurate manipulation of droplets, and then complete the DNA end repair operation. The microdroplet manipulation technology applies the dielectric wetting effect. The dielectric wetting effect refers to that when a small droplet contacts a solid surface, under the action of the electric field formed on the medium surface, the surface charge of the droplet is unevenly distributed, which shows a wetting phenomenon. Therefore, the electrode sheet matrix can make the surface of the droplet unbalanced by adjusting the parameters such as the strength and frequency of the electric field, so as to move, rotate or gather in various directions.

[0056] In the embodiment, the analysis mechanism 2 includes a processor 21 and a chip data board 22 electrically connected with the processor 21, and the processor 21 is provided with a support seat 5 at the bottom. The support seat 5 has a base plate 51 and a base 52, the processor 21 is installed on the base plate 51, and the chip data board 22 is installed on the base 52.

[0057] The support seat 5 is a double-layer upper and lower structure, the base plate 51 is located above the base 52, and is fixedly connected with the support columns on the four corners of the base 52. The support seat 5 as a whole serves as a support damping mechanism of the data analysis part of the device, for protecting components. The processor 21 is installed on the base plate 51 of the upper layer, and the chip data board 22 is fixed in the middle layer between the base plate 51 and the base 52, and is installed in the protection mechanism 4 together with the support seat 5. The processor 21 is built-in with analysis software, which needs to be connected with external devices such as a display and an input device for use, and can collect main data of sequencing, such as running data of the sequencing mechanism 1 and perform data analysis. The processor 21 is connected with the nanopore sequencer through a USB line, and is connected with the display through a DP-to-HDMI adapter. The chip data board 22 is a data collection center of the microfluidic chip 31, and is used for data analysis of the microfluidic chip 31.

[0058] In the embodiment, a single-chip microcomputer is fixed on the chip data board 22, and the single-chip microcomputer is electrically connected with the processor 21.

[0059] Specifically, the processor 21 adopts a jetson orin nano model to manage running data of each process; the single-chip microcomputer can adopt an arduino nano model, which is a controller of the chip data board 22 and can further collect running information of the microfluidic chip 31. The chip data board 22 is provided with a serial interface, and the processor 21 realizes serial communication connection between the chip data board 22 and the single-chip microcomputer. The single-chip microcomputer is connected with an indicator light and a cooling fan through wires, and air inlets and air outlets and the indicator light are correspondingly formed on the shell, so as to reduce the temperature of components and increase visual prompts to improve the use effect of the device.

[0060] As shown in the figure, Figure 5 In the embodiment, a power management system is further included, and the power management system includes an AC 220V-to-DC 19V transformer, a 19V-to-12V transformer and a 12V-to-50-500V adjustable transformer.

[0061] Specifically, the AC 220V-to-DC 19V transformer is connected with a power supply, for supplying power to the processor 21; the 19V-to-12V transformer is electrically connected with the AC 220V-to-DC 19V transformer through wires, for supplying power to the single-chip microcomputer. The 12V-to-50-500V adjustable transformer is connected with the 19V-to-12V transformer through wires, for supplying power to the electrode sheet matrix, so that the electrode sheet matrix drives droplets by adjusting parameters related to the electric field or controlling the on-off sequence of high-voltage current according to the dielectric wetting principle, to realize reagent movement.

[0062] In the embodiment, the protection mechanism 4 is provided with a socket 41, and the socket 41 is clamped with the pretreatment mechanism 3.

[0063] It needs to be explained that the socket 41 is the installation position of the pretreatment mechanism 3, which is provided as an arc-shaped cover structure with one side opening at the top of the shell, and the pretreatment mechanism 3 can be disassembled and installed at any time through the clamping of the socket 41, which is convenient for replacement and assembly. Further, a limiting seat is configured in the socket 41, and three limiting blocks for limiting movement in three directions are distributed on the limiting seat, and the microfluidic chip 31 can be placed on the limiting seat to cooperate with the socket 41. Secondly, the microfluidic chip 31 can be transported separately from the device body when transporting the device, which can avoid accidental damage to the microfluidic chip 31 during transportation, thereby reducing losses. The device body is the protection mechanism 4 and the various components therein, and the remaining part of the device except the sequencing mechanism 1 and the pretreatment mechanism 3.

[0064] In the embodiment, the protection mechanism 4 is provided with an installation slot 42, and the sequencing mechanism 1 is embedded in the installation slot 42.

[0065] It can be understood that the installation slot 42 is located at the top of the protection mechanism 4 and matches the sequencing mechanism 1, and is used for limiting the sequencing mechanism 1. The sequencing mechanism 1 is placed in the installation slot 42 in an embedded manner, so as to be detachably connected with the device body. Similarly, the installation slot 42 is designed in the same way as the socket 41, so that the sequencing mechanism 1 can be transported independently of the device body, thereby reducing the possibility of collision damage.

[0066] The above only describes the preferred embodiments of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. An integrated sequencing and analysis device comprising a sequencing mechanism (1) and an analysis mechanism (2) electrically connected to the sequencing mechanism (1), characterized in that: Also include a pre-processing mechanism (3), the pre-processing mechanism (3) is electrically connected with the sequencing mechanism (1), the analysis mechanism (2); the sequencing mechanism (1) receives the sample after the pre-processing mechanism (3) processes the sample solution; The pre-processing mechanism (3) includes a microfluidic chip (31), and the microfluidic chip (31) is provided with an electrode sheet matrix; Also include a protection mechanism (4), the sequencing mechanism (1), the analysis mechanism (2) and the pre-processing mechanism (3) are arranged in the protection mechanism (4).

2. The sequencing-analysis integrated device of claim 1, wherein: The sequencing mechanism (1) is provided with a sequencing flow channel, the sequencing flow channel is provided with a nanopore, and the sequencing flow channel has a liquid inlet and a liquid outlet, wherein the liquid inlet is provided with a sealing ring.

3. The sequencing-analysis integrated device of claim 1, wherein: The analysis mechanism (2) includes a processor (21) and a chip data board (22) electrically connected with the processor (21), and the processor (21) is provided with a support seat (5) at the bottom; the support seat (5) has a base plate (51) and a base (52), the processor (21) is installed on the base plate (51), and the chip data board (22) is installed on the base (52).

4. The sequencing-analysis integrated device of claim 2, wherein: The sequencing mechanism (1) is also provided with a signal amplification unit, which amplifies the current signal when the nanopore forms a micro-current.

5. The sequencing-analysis integrated device of claim 3, wherein: The chip data board (22) is fixed with a single-chip microcomputer, and the single-chip microcomputer is electrically connected with the processor (21).

6. The sequencing-analysis integrated device of claim 1, wherein: The protection mechanism (4) is provided with a socket (41), and the socket (41) is clamped with the pre-processing mechanism (3).

7. The sequencing-analysis integrated device of claim 1, wherein: The protection mechanism (4) is provided with a mounting groove (42), and the sequencing mechanism (1) is embedded in the mounting groove (42).

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