Rice stalk culture immobilization device for laboratory

Abstract

The utility model discloses a rice stalk culture immobilization device for a laboratory. The rice stalk culture immobilization device comprises a flower mud fixing frame, a replaceable flower mud point hole sheet and a lifting clamping fixing mechanism, the flower mud fixing frame comprises a bottom plate with holes, and the two side ends of the bottom plate with the holes are bent upwards to form U-shaped groove structures used for containing flower mud. The outer contour of the replaceable flower mud point hole sheet is consistent with the contour of the opening in the flower mud fixing frame, multiple rows of staggered point hole through holes are formed in the surface of the replaceable flower mud point hole sheet, and the proper replaceable flower mud point hole sheet is selected according to the size of a plant stem; the lifting clamping and fixing mechanism is arranged above the flower mud fixing frame, the clamping height is adjusted according to the height of a plant stalk, and the clamping distance is adjusted according to the size of the plant stalk. According to the device, through the dual design of hole dotting and flower mud fixing, stable supporting and standardized arrangement of rice in-vitro stalks are achieved, and the phenomenon that disease tissues between different stalks make contact with each other and interfere with each other in the experiment process is effectively reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of plant pathology experiment technology especially to a rice stem culture and solid carrier device for laboratory. BACKGROUND

[0002] Rice stem is the main support structure of rice plant, responsible for transporting water and nutrients, and supporting organs such as leaves and ears.

[0003] Rice sheath blight is one of the main diseases affecting rice yield and green development, and its pathogenic bacteria mainly infect rice sheath, followed by leaves, leading to the inability of photosynthetic products to effectively transfer from leaves, ultimately affecting grain filling and seed setting; Therefore, it is of great significance to accurately identify the resistance level of rice to sheath blight and screen resistant varieties for production and further breeding.

[0004] Existing rice sheath blight resistance identification is mostly carried out in the field, which is greatly affected by environment and human factors, leading to difficulty in accurately obtaining phenotype data, low identification efficiency and poor repeatability. Although there have been a few reports on the study of sheath blight resistance using in vitro stems in the laboratory, the lack of standardized solid carrier device for in vitro culture of rice stems often causes the stems to tilt during growth and interfere with the identification results, ultimately affecting the stability of the identification results, especially limiting the high-throughput, standardized and accurate inoculation identification of a large number of rice samples. SUMMARY

[0005] The utility model discloses a rice stem culture and solid carrier device for laboratory.

[0006] Technical scheme: the rice stem culture and solid carrier device for laboratory, comprising: flower mud fixing frame, replaceable flower mud dot hole sheet, lifting clamping fixing mechanism,

[0007] The flower mud fixing frame comprises a hole bottom plate, and the hole bottom plate is bent upwards at both ends to form a U-shaped groove structure for placing flower mud;

[0008] The replaceable flower mud dot hole sheet has an outer contour consistent with the opening contour of the flower mud fixing frame, and a plurality of rows of staggered dot hole through holes are formed on the surface, and the appropriate replaceable flower mud dot hole sheet is selected according to the size of the plant stem;

[0009] The lifting clamping fixing mechanism is arranged above the flower mud fixing frame, the clamping height is adjusted according to the height of the plant stem, and the clamping distance is adjusted according to the size of the plant stem.

[0010] Further, the flower mud fixing frame is provided with flexible bending clamping plates at both ends, and the flower mud is fixed by bending the flexible bending clamping plates after being placed on the flower mud fixing frame.

[0011] Further, the lifting clamping fixing mechanism comprises support plates arranged at the two end sides of the flower mud fixing frame, a telescopic column is vertically arranged on the support plate, the upper end of the telescopic column is arranged on the fixing plate, and a clamping fixing device is arranged between the two fixing plates.

[0012] Further, the telescopic column comprises a telescopic outer column fixed with the support plate and a telescopic inner column fixed with the fixing plate, a clamping rod is arranged on the side of the telescopic inner column and is connected through a spring and is radially outwardly extruded, and a clamping hole for clamping the clamping rod is arranged on the telescopic outer column in vertical direction and is spaced apart from the clamping rod, and the clamping rod is unlocked or not by pressing or not.

[0013] Further, the clamping fixing device comprises two clamping plates arranged at intervals, the two ends of the clamping plates are connected through U-shaped limiting plates, and the connecting positions are sequentially penetrated by threaded rotating columns, the holes, through which the clamping plates are connected with the threaded rotating columns, are threaded holes matched with the threaded rotating columns, the threaded directions of the two clamping plates arranged at intervals are opposite, and the two clamping plates are close to or away from each other by rotating the threaded rotating columns.

[0014] Further, the opposite surfaces of the two clamping plates arranged at intervals are respectively provided with clamping air bags.

[0015] Advantages: compared with the prior art, the device has the following advantages:

[0016] 1. The double design of the point hole and the fixed flower mud realizes stable support and standardized arrangement of the detached stems of the rice, effectively reduces the mutual contact interference phenomenon of the disease tissues between different stems in the experimental process, and improves the acquisition efficiency and accuracy of the disease resistance phenotype identification result.

[0017] 2. The clamping fixing device can further support and fix the rice stems, prevent the rice stems from tilting or lodging, and ensure the upright growth, and the clamping fixing device can be applied to rice stems of different sizes through the adjustable design, thereby improving the practicability of the device. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a combined schematic view of the flower mud fixing frame and the replaceable flower mud point hole piece of the utility model;

[0019] Figure 2 It is a combined schematic view of the flower mud fixing frame and the lifting clamping fixing mechanism of the utility model;

[0020] Figure 3 It is a telescopic column structure schematic view of the utility model;

[0021] Figure 4 It is a clamping fixing device structure schematic view of the utility model. DETAILED DESCRIPTION

[0022] The technical scheme of the utility model will be further explained in connection with the drawings and embodiments.

[0023] As Figures 1-4 shown, the rice stem culture immobilization device for laboratory includes: flower mud fixing frame 1, replaceable flower mud dot hole sheet 2, lifting clamping fixing mechanism 3.

[0024] The flower mud fixing frame 1 includes a hole bottom plate, the both sides of the hole bottom plate are bent upwards to form a U-shaped groove structure for placing flower mud; the both sides of the flower mud fixing frame 1 are provided with flexible bent clamping plates 101, after the flower mud is placed in the flower mud fixing frame 1, the flower mud can be fixed by bending the flexible bent clamping plates 101. Holes are arranged on the bottom plate and the side plate, after being placed in the culture dish, the nutrient solution enters the flower mud through the holes.

[0025] The replaceable flower mud dot hole sheet 2 has the same outer contour as the opening contour of the flower mud fixing frame 1, a plurality of rows of staggered dot hole through holes 201 are arranged on the surface, the appropriate replaceable flower mud dot hole sheet 2 is selected according to the size of the plant stem, and the flower mud fixing frame 1 and the replaceable flower mud dot hole sheet 2 are combined as shown. Figure 2

[0026] The lifting clamping fixing mechanism 3 is arranged above the flower mud fixing frame 1, the clamping height is adjusted according to the height of the plant stem, and the clamping spacing is adjusted according to the size of the plant stem.

[0027] As Figure 1 shown, the lifting clamping fixing mechanism 3 includes support plates 301 arranged at both ends of the flower mud fixing frame 1, a telescopic column 302 is vertically arranged on the support plate 301, the upper end of the telescopic column 302 is arranged on a fixed plate 303, and a clamping fixing device 304 is arranged between the two fixed plates 303.

[0028] The telescopic column 302 includes a telescopic outer column 302-1 fixed with the support plate 301 and a telescopic inner column 302-2 fixed with the fixed plate 303, a clamping rod 302-3 connected by a spring and radially extruded outward is arranged on the side of the telescopic inner column 302-2, a clamping hole 302-4 for clamping the clamping rod 302-3 is arranged on the vertical diameter of the telescopic outer column 302-1, the locking and unlocking of the clamping rod 302-3 are realized by pressing or not, after unlocking, the telescopic inner column 302-2 is pulled to make the clamping rod 302-3 clamped in other clamping holes 302-4, so that the height of the telescopic column 302 is adjusted. In this way, the plant stems of different heights can be adapted.

[0029] ​The clamping fixing device 304 comprises two clamping plates 304-1 arranged at intervals, two ends of the clamping plates 304-1 are connected through U-shaped limiting plates 304-2, and the connecting positions are sequentially penetrated by threaded rotating columns 304-3. The holes, in which the clamping plates 304-1 are connected with the threaded rotating columns 304-3, are threaded holes matched with the threaded rotating columns 304-3, the threaded directions of the two clamping plates 304-1 corresponding to each other are opposite, and the two clamping plates 304-1 are close to or far away from each other by rotating the threaded rotating columns 304-3. The opposite surfaces of the two clamping plates 304-1 are respectively provided with clamping air bags 304-4, and the contact area of the thicker rice stems with the air bags 304-4 is greater than that of the thin rice stems, so as to adapt to stems with different thicknesses.

Claims

1. A rice stem culture immobilization device for a laboratory, characterized by, The utility model relates to a flower soil fixing frame (1), replaceable flower soil dot hole sheet (2), lifting clamping fixing mechanism (3) are included. The flower soil fixing frame (1) includes a hole bottom plate, the both sides of the hole bottom plate are bent upwards to form a U-shaped groove structure for placing flower soil. The replaceable flower soil dot hole sheet (2) is consistent with the opening contour of the flower soil fixing frame (1) in the outer contour, a plurality of rows of staggered dot hole through holes (201) are arranged on the surface, and the appropriate replaceable flower soil dot hole sheet (2) is selected according to the size of the plant stem. The lifting clamping fixing mechanism (3) is arranged above the flower soil fixing frame (1), the clamping height is adjusted according to the height of the plant stem, and the clamping spacing is adjusted according to the size of the plant stem. The both sides of the flower soil fixing frame (1) are provided with flexible bending clamping plates (101), and after the flower soil is placed in the flower soil fixing frame (1), the flexible bending clamping plates (101) can be bent to fix the flower soil.

2. The rice stem culture immobilization device for a laboratory according to claim 1, characterized by: The lifting clamping fixing mechanism (3) includes support plates (301) arranged at the both ends of the flower soil fixing frame (1), the support plates (301) are vertically provided with telescopic columns (302), the telescopic columns (302) are arranged on the fixed plates (303) at the upper ends, and clamping fixing devices (304) are arranged between the two fixed plates (303).

3. The rice stem culture immobilization device for a laboratory according to claim 1, characterized by: The telescopic column (302) includes a telescopic outer column (302-1) fixed with the support plate (301) and a telescopic inner column (302-2) fixed with the fixed plate (303), the telescopic inner column (302-2) is provided with clamping rods (302-3) connected by springs and radially extruded outward, the telescopic outer column (302-1) is provided with clamping holes (302-4) for clamping the clamping rods (302-3) and spaced apart vertically corresponding to the clamping rods (302-3), and the clamping rods (302-3) are unlocked or not by pressing.

4. The rice stem culture immobilization device for a laboratory according to claim 3, characterized by: The clamping fixing device (304) includes two clamping plates (304-1) arranged at intervals, the both ends of the clamping plates (304-1) are connected by U-shaped limiting plates (304-2), the connecting parts are sequentially penetrated by threaded rotating columns (304-3), the holes of the clamping plates (304-1) and the threaded rotating columns (304-3) are threaded holes matched with the threaded rotating columns (304-3), the threaded directions of the two clamping plates (304-1) arranged at intervals are opposite, and the two clamping plates (304-1) are close to or away from each other by rotating the threaded rotating columns (304-3).

5. The rice stem culture immobilization device for a laboratory according to claim 3, characterized by: The opposite surfaces of the two clamping plates (304-1) arranged at intervals are respectively provided with clamping air bags (304-4).

6. The rice stem culture immobilization device for a laboratory according to claim 5, characterized by: ​

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