Conical flask convenient for disinfecting tissue culture experiment seeds

Abstract

The utility model relates to the technical field of plant tissue culture, and discloses a conical flask convenient for disinfecting tissue culture experiment seeds, which comprises a flask body and a lower flask cover, the flask body and the lower flask cover are detachably arranged, a plurality of separation columns are vertically arranged on one side of the lower flask cover facing the flask body, and all the separation columns are arranged at intervals. According to the utility model, the plurality of separation columns are arranged at the bottom of the tissue culture bottle, so that in the process of shaking the tissue culture bottle, seeds which are bonded into clusters collide with the separation columns under the action of inertia and penetrate through gaps among the separation columns so as to be separated from one another, and the uniformity of seed disinfection is ensured.

Description

Technical Field

[0001] This utility model relates to the field of plant tissue culture technology, and in particular to a conical flask for convenient disinfection of seeds in tissue culture experiments. Background Technology

[0002] Plant tissue culture technology is widely used in the field of agricultural biotechnology. In the process of plant tissue culture, researchers need to cultivate and propagate plant seeds or seedlings in sterile culture containers to achieve plant seedling regeneration. In tissue culture, tissue culture bottles, as glass packaging containers, are non-toxic, safe, hygienic, and have excellent transparency and light transmittance, playing a crucial role in the cultivation of plant tissues.

[0003] In plant tissue culture experiments, seed sterilization is a crucial step that directly affects the subsequent germination rate. Currently, ordinary Erlenmeyer flasks are commonly used for seed sterilization in tissue culture experiments. However, these traditional Erlenmeyer flasks have several drawbacks in practice. First, during the manual shaking process to ensure full contact between the seeds and the sterilizing solution, the moistened seeds tend to clump together, making it difficult to ensure uniform sterilization. Second, when pouring out the sterilizing solution after sterilization, the process is slow and seeds are easily spilled out along with the solution. The same issue occurs when emptying the washing solution after rinsing the tissue culture flask and seeds. Third, the narrow opening of the Erlenmeyer flask makes it difficult to remove seeds as they tend to stick to the flask wall, and adding sterilizing solution can easily cause splashing and leakage. Therefore, improvements to the existing Erlenmeyer tissue culture flasks are needed. Utility Model Content

[0004] To address the problems existing in the prior art, where moist seeds in tissue culture flasks tend to clump together during sterilization, leading to uneven sterilization, this invention aims to provide a conical flask for convenient seed sterilization in tissue culture experiments. By setting several separation columns at the bottom of the flask, the clumps of seeds collide with the separation columns due to inertia during shaking, passing through the gaps between the columns and separating them, thus ensuring uniform seed sterilization.

[0005] To achieve the above objectives, the technical solution of this utility model is as follows:

[0006] A conical flask for convenient seed sterilization in tissue culture experiments includes a flask body and a lower flask cap. The flask body and the lower flask cap are detachably connected. Several separation columns are provided on the side of the lower flask cap facing the flask body. The separation columns are vertically arranged and all separation columns are spaced apart.

[0007] The present invention is further configured such that the separation column is cylindrical.

[0008] The present invention is further configured such that the separating column is located at the center of the lower bottle cap.

[0009] The present invention is further configured to include a seed filter mechanism, the seed filter mechanism including a filter screen, the filter screen being horizontally disposed inside the bottle body and located above the separation column, the filter screen being circular and the edge of the filter screen being in close contact with the inner wall of the bottle body.

[0010] The present invention is further configured such that: the seed filter mechanism further includes a retainer, the height of the retainer is greater than the height of the separation column, and the filter screen is fixed to the top of the retainer.

[0011] The present invention is further configured such that: the retainer includes a support ring and a connector, the support ring is located below the filter screen, and the two ends of the connector are fixedly connected to the support ring and the filter screen respectively.

[0012] The present invention is further configured such that the seed filter mechanism is in the shape of a frustum and the shape and size of the seed filter mechanism are adapted to the bottle body.

[0013] The present invention is further configured such that: the retainer also includes a handle for human hand gripping, the handle being disposed on the support ring.

[0014] The present invention is further configured such that the number of the connectors is not less than two.

[0015] The present invention is further configured to include an upper bottle cap, wherein the bottle body and the upper bottle cap are detachably connected.

[0016] In summary, the beneficial effects achieved by this utility model are as follows:

[0017] (1) During the disinfection process of manually shaking the conical flask to ensure that the seeds are in full contact with the disinfectant, the seeds that are piled up and stuck together collide with multiple spaced separation columns under the action of inertia. The clumps of seeds are broken up and pass through the gaps between the separation columns, thus separating them from each other. The scattered seeds can then come into full contact with the disinfectant, thereby ensuring the uniformity of seed disinfection.

[0018] (2) The separation column is located in the center of the lower bottle cap, which can increase the probability of the seed colliding with the separation column. The cylindrical separation column is beneficial to protect the seed from being scratched by sharp edges during the collision.

[0019] (3) The seed filter mechanism can prevent the seeds inside the tissue culture bottle from being poured out simultaneously while the disinfectant or other cleaning solution is being poured out of the bottle;

[0020] (4) The retainer consisting of a support ring and a connector is used to fix the filter screen, which solves the problem of fixing the filter screen inside the bottle and allows the entire seed filter mechanism to be removed after the filtration operation is completed, thus avoiding the seed filter mechanism from affecting the tissue culture experiment.

[0021] (5) The bottom cap of the bottle is detachable, and the seeds, disinfectant and the frustum-shaped seed filter can all enter the tissue culture bottle from the bottom cap. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the specification will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0023] Figure 1 A schematic diagram of the conical flask used in this utility model for convenient seed sterilization in tissue culture experiments;

[0024] Figure 2 This is a top view of the lower cap of the conical bottle in this utility model;

[0025] Figure 3 This is a front view of the seed filter mechanism of the conical bottle in this utility model;

[0026] Figure 4 This is a top view of the seed filter mechanism of the conical bottle in this utility model.

[0027] In the diagram: 1. Upper bottle cap; 2. Bottle body; 3. Lower bottle cap; 31. Separation column; 4. Seed filter mechanism; 41. Filter screen; 42. Connector; 43. Support ring; 44. Handle. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, not all embodiments. For ease of explanation, the terms "vertical", "horizontal", "left", "right", "upper", "lower", "inner", "outer", "bottom", etc., used in this specification indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0029] Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0030] As attached Figure 1-4 As shown, a conical flask for convenient seed sterilization in tissue culture experiments includes an upper cap 1, a flask body 2, a lower cap 3, and a seed filter mechanism 4.

[0031] The conical bottle body 2 is cone-shaped with a cylindrical section vertically set at the top. The upper bottle cap 1 is placed on the cylindrical section. The bottle body 2 and the upper bottle cap 1 are detachable, so that the conical bottle can be opened or closed from the top.

[0032] The edge of the lower cap 3 is slightly higher than the center, allowing it to hold more disinfectant or seeds. The bottle body 2 and the lower cap 3 are also detachable, allowing the conical flask to be opened or closed from the bottom.

[0033] Several separation columns 31 are provided on the side of the lower bottle cap 3 facing the bottle body 2.

[0034] The separating columns 31 are cylindrical and vertically distributed at the center of the lower bottle cap 3. In this embodiment, a total of 12 separating columns 31 are provided, and all separating columns 31 are spaced apart and evenly distributed. In other embodiments, the number and position of the separating columns 31, as well as the distance between them, can be adaptively adjusted according to the size of the seeds, so that the distance between two adjacent separating columns 31 is greater than the maximum size of the seeds.

[0035] The seed filter mechanism 4 is located inside the bottle body 2 and is used to prevent seeds from being poured out simultaneously during the pouring of disinfectant or cleaning solution from the conical flask. The seed filter mechanism 4 includes a filter screen 41 and a retainer, and the retainer includes a connector 42, a support ring 43, and a handle 44.

[0036] The filter screen 41 is circular, and its mesh size is smaller than the minimum size of the seed. The filter screen 41 is horizontally fixed to the top of the holder, and the edge of the filter screen 41 is in close contact with the inner wall of the bottle body 2 to prevent the seed from passing through the gap between the filter screen 41 and the inner wall of the bottle body 2.

[0037] The support ring 43 is circular in shape, horizontally positioned on the lower bottle cap 3 and directly below the filter screen 41; the two ends of the connector 42 are fixedly connected to the support ring 43 and the filter screen 41 respectively, thereby providing stable support for the filter screen 41. The connector 42 is elongated, and there are at least two of them; in other embodiments, three or four may be evenly distributed.

[0038] The diameter of the support ring 43 is larger than the diameter of the filter screen 41, and their axes coincide. The two ends of the connector 42 are fixedly connected to the edges of the support ring 43 and the filter screen 41, respectively, so that the seed filter mechanism 4 is in the shape of a frustum. Furthermore, the shape and size of the seed filter mechanism 4 are adapted to the bottle body 2, so that the seed filter mechanism 4 can enter the interior of the bottle body 2 from the bottom of the bottle body 2 and the filter screen 41 can be kept horizontal while its edge can be in close contact with the inner wall of the bottle body 2.

[0039] The height of the retainer is greater than the height of the separation column 31, thereby avoiding interference between the separation column 31 and the filter screen 41.

[0040] Handle 44 is used for gripping to remove the filter mechanism 4 from the conical flask. Handle 44 is semi-circular in shape, with both ends hinged to the support ring 43, so that handle 44 can be stored in the internal space of the support ring 43 and can be pulled out when in use.

[0041] The implementation principle of the above embodiments is as follows:

[0042] When using the conical flask of this invention, open the lower cap 3, and the seeds, disinfectant, and seed filter mechanism 4 can enter the bottle body 2 from the bottom. Then close the upper cap 1 and the lower cap 3, and shake the bottle body 2 to disinfect the seeds. During disinfection, the clumps of seeds, due to inertia, continuously collide with the multiple spaced separating columns 31, breaking up the clumps and allowing them to pass through the gaps between the columns 31, thus separating them. The scattered seeds come into full contact with the disinfectant, resulting in uniform seed disinfection. After disinfection, open the upper cap 1, and the disinfectant can be poured out from the top opening of the bottle body 2. During pouring, the filter screen 41 prevents the seeds from being poured out with the disinfectant. After the seeds have been disinfected and cleaned, open the lower cap 3, and remove the seed filter mechanism 4 using the handle 44 to retrieve the clean seeds.

[0043] Although preferred embodiments of the present invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including both the preferred embodiments and all changes and modifications falling within the scope of the present invention. Clearly, those skilled in the art can make various alterations and modifications to the present invention without departing from its spirit and scope. Thus, if such modifications and modifications fall within the scope of the claims of the present invention and their equivalents, the present invention also intends to include such modifications and modifications.

Claims

1. A conical flask for facilitating seed sterilization for tissue culture experiments, characterized in that, The application relates to a bottle, which comprises a bottle body (2) and a lower bottle cap (3), the bottle body (2) is detachably arranged with the lower bottle cap (3), a plurality of separation columns (31) are arranged on the side of the lower bottle cap (3) facing the bottle body (2), and the separation columns (31) are vertically arranged and are arranged at intervals.

2. The conical flask for convenient aseptic culture experiment of seed according to claim 1, characterized in that, The separation columns (31) are in the shape of a cylinder.

3. The conical flask for convenient tissue culture experiment seed disinfection according to claim 1, characterized in that, The separation columns (31) are arranged at the central position of the lower bottle cap (3).

4. The conical flask for convenient set-up of seed sterilization experiments according to claim 1, characterized in that, The application further relates to a filter mechanism (4), which comprises a filter screen (41), the filter screen (41) is horizontally arranged in the bottle body (2) and is arranged above the separation columns (31), the filter screen (41) is in the shape of a circle and the edge of the filter screen (41) is in close contact with the inner wall of the bottle body (2).

5. The conical flask for convenient set-up of seed sterilization experiments according to claim 4, characterized in that, The filter mechanism (4) further comprises a holder, the height of the holder is greater than the height of the separation columns (31), and the filter screen (41) is fixed to the top end of the holder.

6. The conical flask for convenient set-up of seed sterilization experiments according to claim 5, characterized in that, The holder comprises a supporting ring (43) and a connecting piece (42), the supporting ring (43) is arranged below the filter screen (41), and the two ends of the connecting piece (42) are fixedly connected with the supporting ring (43) and the filter screen (41) respectively.

7. The conical flask for convenient set-up of seed sterilization experiments according to claim 6, characterized in that, The filter mechanism (4) is in the shape of a circular truncated cone and the shape and size of the filter mechanism (4) are matched with the bottle body (2).

8. The conical flask for convenient set-up of seed sterilization experiments according to claim 6, characterized in that, The holder further comprises a handle (44) for being gripped by a hand, and the handle (44) is arranged on the supporting ring (43).

9. The conical flask for convenient set-up of seed sterilization experiments according to claim 6, characterized in that, The number of the connecting pieces (42) is not less than two.

10. The conical flask for convenient tissue culture experiment seed disinfection according to claim 1, characterized in that, The application further relates to an upper bottle cap (1), and the bottle body (2) is detachably arranged with the upper bottle cap (1).

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