Slice storage box

Abstract

The utility model relates to a slice storage box which comprises a slice box, the slice box is provided with at least two containing cavities and an anti-freezing solution cavity, and the anti-freezing solution cavity communicates with the multiple containing cavities through exchange holes. The plurality of accommodating cavities in the slice box are used for storing slices of different layers of mouse brain tissues, so that the number of the slices in a single accommodating cavity is reduced, and the slices are convenient to pick; and meanwhile, the plurality of accommodating cavities are integrated in the same slice box, and the relative positions of the accommodating cavities are fixed, so that the frozen slices at different layers are prevented from being lost and mixed. The anti-freezing solution exchange between the multiple containing cavities and the anti-freezing solution cavity is achieved through the exchange holes, compared with a method of directly pouring the anti-freezing solution, the anti-freezing solution cavity plays a buffering role, impact force generated by pouring the anti-freezing solution is absorbed and then slowly enters the containing cavities through the exchange holes, damage to the slices is effectively weakened, and the anti-freezing solution is prevented from being damaged. Meanwhile, the damage caused by the fact that the slice is mistakenly sucked into the pipette tip is also avoided.

Description

Technical Field

[0001] This application relates to the field of medical devices, specifically to a slide storage box. Background Technology

[0002] Whole-brain slices in mice are a key experimental tool in neuroscience research. Whole-brain slices allow for simultaneous observation of features across multiple brain regions, providing comprehensive brain information while preserving the structural relationships between different brain areas and avoiding spatial localization errors that may occur with localized slices. Numerous sequential slices can be used to reconstruct the three-dimensional structure of the mouse brain, enabling the study of cross-regional neural pathways, long-distance connections, and brain region interactions. This allows researchers to meticulously observe the relative positions and structural relationships of different brain regions. Through this method, researchers can gain a deeper understanding of the complex structure, neural networks, and pathological changes of the mouse brain, providing extremely rich and reliable experimental data support for the field of neuroscience.

[0003] To protect the enzyme and antigen activities of mouse brain tissue sections and inhibit fungal growth, freezing is often necessary. However, frozen sections present several inconveniences in use. First, due to the large number of sections, picking them out after freezing is difficult. Second, storing sections of different layers in separate centrifuge tubes can easily lead to loss or confusion. Third, adding antifreeze requires injecting it into separate centrifuge tubes, making the process complex. Finally, during antifreeze aspiration, sections may be accidentally drawn into the pipette tip due to negative pressure, damaging the sections, and excessive impact during antifreeze injection can also damage them. Utility Model Content

[0004] To solve the above-mentioned technical problems, this application provides the following technical solution:

[0005] A slice storage box includes: a slice box having at least two accommodating cavities and an antifreeze cavity, the antifreeze cavity being connected to multiple accommodating cavities through exchange holes, and the slice box also having a filling module, the filling module including a liquid inlet formed on the surface of the slice box, the liquid inlet being connected to the antifreeze cavity.

[0006] In one embodiment, it further includes: a sealing cap, the sealing cap including a base plate and a plurality of caps disposed on the base plate, the caps being disposed corresponding to the receiving cavity, the base plate having a base layer and a marking layer, the caps and the marking layer being disposed on both sides of the base layer.

[0007] In one embodiment, the accommodating cavity array is distributed in the slice box, and the cover is arranged in a straight line on the base plate.

[0008] In one embodiment, the base plate includes the same number of sub-plates as the cover, the sub-plates are arranged in a straight line, and the cover is arranged in a one-to-one correspondence with the sub-plates; the sub-plates are connected by flexible folded edges.

[0009] In one embodiment, the base plate and the slice box are connected by a flexible connecting strip.

[0010] In one embodiment, multiple sealing caps and slice boxes are connected in series via flexible connecting strips.

[0011] In one embodiment, the marking layer is a frosted layer.

[0012] In one embodiment, the receiving cavity is located inside the antifreeze cavity, and the receiving cavity and the antifreeze cavity are separated by a partition, with the exchange hole opened on the partition.

[0013] In one embodiment, the filling module further includes a sealing cap that covers the inlet.

[0014] This application has at least the following beneficial effects:

[0015] In this application, multiple cavities on the slide box are used to store slides of different layers of mouse brain tissue, reducing the number of slides in a single cavity to facilitate slide selection. At the same time, since multiple cavities are integrated on the same slide box, the relative positions between the cavities are fixed, avoiding the loss and confusion of slides of different layers after freezing.

[0016] In this application, the exchange port enables the exchange of antifreeze between multiple communal chambers and the antifreeze chamber. Compared with the method of directly drawing and pouring antifreeze, the antifreeze chamber acts as a buffer, absorbing the impact force of pouring antifreeze before slowly entering the communal chamber through the exchange port. This can effectively reduce damage to the slides and prevent the slides from being accidentally drawn into the pipette tip due to negative pressure, thus avoiding damage to the slides. Attached Figure Description

[0017] Figure 1 This is a three-dimensional schematic diagram of the slice storage box provided in Embodiment 1 of this application.

[0018] Figure 2 This is a three-dimensional schematic diagram of the slicing box provided in Embodiment 1 of this application.

[0019] Figure 3 This is a cross-sectional view of the slicing box provided in Embodiment 1 of this application.

[0020] Figure 4 This is a three-dimensional schematic diagram of the sealing cap provided in Embodiment 1 of this application.

[0021] Figure 5This is a front view of the sealing cap provided in Embodiment 1 of this application.

[0022] Figure 6 This is a three-dimensional schematic diagram of the slice storage box provided in Embodiment 2 of this application.

[0023] Figure 7 This is a three-dimensional schematic diagram of the sealing cap provided in Embodiment 2 of this application.

[0024] Figure 8 This is a front view of the sealing cap provided in Embodiment 2 of this application.

[0025] Figure 9 This is a perspective view of the slice storage box provided in Embodiment 3 of this application.

[0026] Figure label:

[0027] 1. Slicing box;

[0028] 11. Receptacle; 12. Antifreeze chamber; 13. Filling module;

[0029] 111. Exchange port;

[0030] 2. Sealed cover;

[0031] 21. Base plate; 22. Cover; 23. Connecting strip;

[0032] 2101, base layer; 2102, frosted layer; 211, panel; 212, folded edge. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the described embodiments of this application are within the scope of protection of this application.

[0034] Unless otherwise defined, the technical or scientific terms used in this application shall have the ordinary meaning understood by one of ordinary skill in the art to which this application pertains. The terms "first," "second," and similar terms used in this application do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Similarly, the terms "a" or "one," and similar terms do not indicate a quantity limitation, but rather indicate the presence of at least one. The terms "connected" or "linked," and similar terms, are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right," etc., are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship also changes accordingly.

[0035] The embodiments of this application are described in detail below with reference to the accompanying drawings.

[0036] refer to Figure 1-3 As shown, an embodiment of this application provides a slide storage box, including: a slide box 1, which has at least two receiving cavities 11 for storing slides of different layers of mouse brain tissue. The slide box 1 also has an antifreeze chamber 12, which stores antifreeze. The antifreeze chamber 12 is connected to the multiple receiving cavities 11 through exchange holes 111, allowing the antifreeze to enter the receiving cavities 11 via the exchange holes 111, thereby freezing the slides. The specific steps for adding antifreeze include: first, filling the antifreeze chamber 12 through a filling module 13; then, the antifreeze chamber 12 permeates into the receiving cavities 11 through the exchange holes 111, thus completing the injection of antifreeze into the receiving cavities 11. Compared to directly injecting antifreeze into the receiving cavity 11, the operator only needs to fill the antifreeze cavity 12 with antifreeze. Antifreeze can be obtained in multiple receiving cavities 11, thus reducing the number of times the operator needs to fill the cavity repeatedly. In addition, when filling the antifreeze, the antifreeze acts with an impact force due to the gravitational potential energy in the antifreeze cavity 12, while the antifreeze flowing through the exchange hole 111 into the receiving cavity 11 is gentler, reducing the damage to the slide caused by the impact force of the antifreeze.

[0037] Furthermore, the receiving cavity 11 is located within the antifreeze chamber 12, and the receiving cavity 11 and the antifreeze chamber 12 are separated by a partition, with the exchange hole 111 located on the partition. Specifically, the receiving cavity 11 is located within the antifreeze chamber 12, with the antifreeze chamber 12 surrounding the circumference of the receiving cavity 11, and the top of the receiving cavity 11 exposed to the external environment; that is, the top opening of the receiving cavity 11 is not surrounded by the antifreeze chamber 12. For example, the partition has several recessed grooves, which serve as the receiving cavities 11, extending into the antifreeze chamber 12 such that the bottom of the recessed grooves is close to the bottom of the antifreeze chamber 12, and the exchange hole 111 is located at the bottom of the grooves. When the liquid level in the antifreeze chamber 12 is higher than the bottom of the recessed grooves, antifreeze slowly enters the receiving cavity 11 through the exchange hole 111. Simultaneously, because the bottom of the recessed grooves is close to the bottom of the antifreeze chamber 12, the amount of antifreeze required to be injected into the antifreeze chamber 12 is reduced. For example, the recessed groove has a diameter of 1.75 cm and a depth of 1 cm. While ensuring the flat unfolding and cryopreservation of coronal and sagittal sections of mouse brain, it minimizes the volume of the well to reduce antifreeze waste. At least two exchange holes 111 are located at the bottom of the groove to prevent antifreeze from failing to enter the receiving cavity 11 if the exchange holes 111 become blocked. For example, seven exchange holes 111 are provided, with one exchange hole 111 located at the center of the groove bottom and the remaining six exchange holes 111 distributed circumferentially near the edge of the groove bottom. The diameter of the exchange holes 111 is 2 mm.

[0038] The receiving cavity 11 is located at the top of the antifreeze cavity 12. Antifreeze is poured into the antifreeze cavity 12 to a certain height, so that the liquid level is higher than the bottom of the tank, and the antifreeze enters the receiving cavity 11.

[0039] The slide container 1 is also equipped with a filling module 13, which is disposed on the surface of the slide container 1 near the recessed groove opening. The filling module 13 also includes a sealing cap that covers the liquid inlet. The filling module 13 includes a liquid inlet formed on the surface of the slide container 1, which communicates with the antifreeze chamber 12. More specifically, the liquid inlet is located at the highest point in the vertical direction of the slide storage container to facilitate the filling of antifreeze. For example, the sealing cap is fitted into the liquid inlet, and the sealing cap can be inserted into the liquid inlet to seal the liquid inlet. Antifreeze is quickly injected or drawn into the antifreeze chamber 12 through the liquid inlet using a pipette, so as to replace or replenish the antifreeze without direct contact with the brain slices, thereby minimizing physical damage to the brain slices.

[0040] In some embodiments of this application, such as Figure 1 and combined Figure 4 , 5As shown, in Embodiment 1, the slice storage box further includes a sealing cap 2. The sealing cap 2 includes a base plate 21 and multiple caps 22 disposed on the base plate 21. The caps 22 are disposed corresponding to the receiving cavities 11, that is, the caps 22 can be inserted into the recessed grooves. The base plate 21 has a base layer 2101 and an identification layer, and the caps 22 and the identification layer are disposed on both sides of the base layer 2101. For example, the sealing cap 2 is a four-cap, which consists of four caps 22 arranged on a base plate 21. In this embodiment, the sealing cap 2 can cover one row of receiving cavities 11.

[0041] However, it is understood that the sealing cap 2 includes, but is not limited to, having four caps 22, and the number of caps 22 on the sealing cap 2 may also be two or three.

[0042] During the use of the slide storage box, it was found that in order to accurately determine the slide information in each accommodating cavity 11, markings were made on the surface of the base plate 21. These markings could be done by affixing labels or writing with a marker. However, because the slide storage box is cryogenically stored, condensation droplets will form on the surface of the storage box during use. Marking on the base plate 21, where condensation is present, will severely affect the marking effect. Specifically, labels are prone to falling off, and marker marks are easily scratched.

[0043] In this design, a marking layer is provided, and markings are made on this layer to ensure the markings are secure. Specifically, the marking layer is a surface layer that increases friction. Preferably, the marking layer is a frosted layer 2102. Increasing friction ensures that the label remains in contact with the frosted layer 2102, preventing it from easily detaching. Furthermore, because the surface of the frosted layer 2102 has a microscopic pitted texture, the ink from the marker pen is contained within these pits and is not easily erased, thus making the written marks more firmly attached to the frosted layer 2102.

[0044] Furthermore, the cavities 11 are arrayed in the slide cassette 1; for example, the cavities 11 are arranged in a 4*3 array. The covers 22 are arranged in a straight line on the base plate 21, allowing the sealing cap 2 to simultaneously cover a row or column of cavities 11, reducing the number of individual covers required to cover each cavity 11. Because the sealing cap 2 has multiple covers 22, the risk of misuse of covers for the cavities 11 is reduced, decreasing the probability of confusion between the marking information and the samples within the cavities 11.

[0045] Furthermore, refer to Figure 6-8As shown, in Embodiment 2, the base plate 21 includes the same number of sub-plates 211 as the cover 22. The sub-plates 211 are arranged in a straight line, and the cover 22 is arranged in a one-to-one correspondence with the sub-plates 211. The sub-plates 211 are connected by flexible folded edges 212. The flexible folded edges 212 can deform to change the angle of two adjacent sub-plates 211. Specifically, the sealing cap 2 can achieve a partial insertion of the cover 22 into the recessed groove, while the other part of the cover 22 is separated from the receiving cavity 11, thereby opening the receiving cavity 11 covered by the sealing cap 2. At the same time, since part of the cover 22 of the sealing cap 2 is inserted into the recessed groove, the sealing cap 2 does not detach from the slice box 1, thus preventing the loss of the sealing cap 2.

[0046] Furthermore, to prevent the sealing cap 2 from being lost, the base plate 21 is connected to the slide box 1. After all the caps 22 on the sealing cap 2 are separated from the receiving cavity 11, the sealing cap 2 remains connected to the slide box 1 to prevent the sealing cap 2 from being lost. In Embodiment 3, refer to Figure 9 As shown, the base plate 21 is connected to the slide box 1 by a flexible connecting strip 23. Each base plate 21 is provided with a connecting strip 23 to connect to the slide box 1, so that the sealing cap 2 is independently connected to the slide box 1.

[0047] The above embodiments are used to further illustrate this application, but do not limit this application to these specific implementations. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be understood as falling within the protection scope of this application.

Claims

1. A slice storage cassette, characterized by, The application relates to a slice box (1) with at least two accommodation cavities (11) and an anti-freezing liquid cavity (12) communicating with the accommodation cavities (11), and a filling module (13) provided on the slice box (1), wherein the filling module (13) comprises a liquid inlet opening on the surface of the slice box (1) and communicating with the anti-freezing liquid cavity (12). The application further relates to a sealing cover (2) comprising a bottom plate (21) and a plurality of cover bodies (22) provided on the bottom plate (21), wherein the cover bodies (22) correspond to the accommodation cavities (11), the bottom plate (21) has a base layer (2101) and an identification layer, and the cover bodies (22) and the identification layer are arranged on two sides of the base layer (2101).

2. The slice storage box of claim 1, wherein, The accommodation cavities (11) are arranged in the slice box (1), and the cover bodies (22) are arranged on the bottom plate (21) along a straight line direction. The bottom plate (21) comprises a plurality of sub-plates (211) corresponding to the cover bodies (22), the sub-plates (211) are arranged along the straight line direction, the cover bodies (22) and the sub-plates (211) are arranged one by one, and the sub-plates (211) are connected through flexible folding edges (212).

3. The slice storage box of claim 2, wherein, The bottom plate (21) and the slice box (1) are connected through a flexible connecting belt (23).

4. The slice storage box of claim 3, wherein, A plurality of the sealing covers (2) and the slice box (1) are connected through the flexible connecting belts (23) in sequence.

5. The slice storage box of claim 4, wherein, The identification layer is a frosted layer (2102).

6. The slice storage box of claim 4, wherein, The accommodation cavities (11) are located in the anti-freezing liquid cavity (12), and the accommodation cavities (11) and the anti-freezing liquid cavity (12) are isolated through a partition plate provided with exchange holes (111).

7. The slice storage magazine of claim 2, wherein, The filling module (13) further comprises a sealing cover covering the liquid inlet opening.

8. The slice storage box of any one of claims 1-4, wherein, ​ 9. The slice storage magazine of claim 1, wherein, ​

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