Culture device capable of measuring bacterial concentration in real time

Abstract

The utility model discloses a culture device capable of measuring bacterial concentration in real time, which comprises a triangular flask, a shaking table, an ultraviolet bacterial concentration measuring component and an oscillating component, a cuvette is arranged at the bottom of the triangular flask, an accommodating space is arranged in the shaking table, the triangular flask is arranged in the shaking table for bacterial culture, a placing plate and a placing seat are arranged in the shaking table, and the shaking table is arranged on the placing plate. A through hole is formed in the placing plate in the thickness direction of the placing plate, the placing seat is located below the placing plate and used in cooperation with the through hole, an iron stand is installed above the placing plate, the triangular flask is installed on the placing plate through the iron stand, the cuvette penetrates through the through hole to be placed in the placing seat, and the ultraviolet bacterium concentration measuring assembly is arranged in the shaking table. And the oscillating assembly is arranged in the shaking table and is connected with the placing plate, so that the shaking culture of the triangular flasks on the placing plate is realized. According to the utility model, the bacterial concentration of a sample can be measured without repeated sampling, so that the experimental pollution caused by sampling for measuring the bacterial concentration is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to experimental apparatus technical field, concretely relates to a culture device of real -time measurement bacterial concentration. BACKGROUND

[0002] In the field of modern biology, medicine and related scientific research, bacterial culture is a basic and crucial experimental operation. As a commonly used cell suspension culture container, the flask provides suitable space for the growth of bacteria with its unique shape and structure, and is widely used in various bacterial culture experiments. At the same time, the shaking table as an important laboratory instrument and equipment, by providing a stable oscillation environment, promotes the full contact of bacteria and culture medium, accelerates the absorption of nutrients and the discharge of metabolic products, and plays an irreplaceable role in bacterial culture, fermentation, hybridization, biochemical reaction and enzyme and tissue research and many other experiments.

[0003] However, at present, in the process of bacterial culture by using the shaking table, there are many problems to be solved in the bacterial concentration measurement link. In the bacterial concentration measurement operation, the traditional method needs to take samples frequently from the flask being cultured. This process is not only tedious, seriously affects the experimental efficiency, but also in the process of multiple sampling, since the operation environment is difficult to be absolutely sterile, foreign bacteria are easily introduced, which pollutes the originally pure culture sample. Once pollution occurs, the whole culture experiment may be in vain, and a lot of time, manpower and material resources are wasted.

[0004] At the same time, the existing bacterial concentration measurement method operation process is extremely complex. From sample taking to completing concentration measurement, it needs to go through multiple steps, involves the use of multiple instruments and equipment and complex calculation process, which often needs to spend a long time. In the process of bacterial culture, the growth rate of bacteria is fast, and the concentration changes rapidly. Since the real-time bacterial concentration data cannot be obtained in time and accurately, the experimenter is difficult to perform the induction and other key operations at the best time. When the measurement time is too long, it is easy to cause the bacterial concentration to exceed the original induction value range, so that the subsequent experimental results deviate or even completely fail, which seriously affects the accuracy and reliability of the experiment.

[0005] Therefore, how to solve the above problems existing in the prior art has become the research subject of the present application. UTILITY MODEL CONTENT

[0006] Therefore, the utility model aims at providing a culture device capable of measuring bacterial concentration in real time.

[0007] To achieve the above purpose, the utility model adopts the technical scheme of:

[0008] A culture device capable of real-time measurement of bacterial concentration includes:

[0009] An Erlenmeyer flask with a cuvette attached to its bottom;

[0010] A shaker has an internal space for accommodating the Erlenmeyer flasks, which are placed inside the shaker for bacterial culture. The shaker is equipped with a placement plate and a placement seat. The placement plate has a through hole along its thickness direction. The placement seat is located below the placement plate and is used in conjunction with the through hole. An iron frame is installed above the placement plate. The Erlenmeyer flasks are mounted on the placement plate through the iron frame. The cuvettes are placed in the placement seat through the through hole.

[0011] An ultraviolet bacterial concentration measuring component is disposed inside the shaker and measures the bacterial concentration of the Erlenmeyer flask.

[0012] An oscillation assembly is disposed inside the shaker and is connected to the placement plate to realize the oscillation culture of the Erlenmeyer flasks on the placement plate.

[0013] Furthermore, the ultraviolet bacterial concentration measurement component includes a light source, a grating monochromator, an absorption cell, and a detector. A lamp source frame is installed on the inner wall of the bottom of the shaker. Both the light source and the grating monochromator are mounted on the lamp source frame. The grating monochromator works in conjunction with the light source to guide the light emitted by the light source into the cuvette. The absorption cell is installed inside the shaker and absorbs light that is not guided into the cuvette. The detector is installed on the inner side wall of the shaker and receives the light that passes through the cuvette.

[0014] Furthermore, the grating monochromator is a rectangular iron box structure, and openings are provided on both sides of the grating monochromator along the direction of light propagation. The two openings serve as the entrance slit for light to enter and the exit slit for light to exit, respectively.

[0015] Light propagates through the entrance slit into the grating monochromator. Inside the grating monochromator, a collimating lens is installed along the light propagation path. The collimating lens reflects the light to the grating inside the grating monochromator. The grating disperses the light. The dispersed light propagates to a focusing mirror inside the grating monochromator. After being focused by the focusing mirror, the light is exited from the exit slit.

[0016] Furthermore, a motor-controlled telescopic rod is installed below the entrance slit, and the motor-controlled telescopic rod is connected to the grating.

[0017] Furthermore, the oscillation assembly includes a movable base, a trapezoidal iron plate, and a compression spring. The movable base is movably connected to the bottom inner wall of the shaker. One end of the trapezoidal iron plate is connected to the placement plate, and the other end is connected to the movable base. One end of the compression spring is connected to the inner wall of the shaker, and the other end is connected to the trapezoidal iron plate.

[0018] Furthermore, the movable base is connected to a rotary motor via a threaded shaft, and the rotary motor drives the movable motor to reciprocate within the shaking table; an elliptical iron block is mounted on the movable base, and the elliptical iron block is connected to the rotary motor via a transmission connection, and the rotary motor controller controls the elliptical iron block to swing on the movable base.

[0019] Furthermore, the shaker is equipped with a partition assembly, which includes a first partition, a second partition, and a stud. The first partition and the second partition are cross-connected, and the stud is installed at the connection point. Both ends of the first partition and the second partition have openings along their length.

[0020] Furthermore, the placement plate includes a first placement plate, a second placement plate, and a third placement plate. The first placement plate and the second placement plate are symmetrically arranged on the inner walls of both sides inside the shaker, and the third placement plate is movably connected to the first placement plate and the second placement plate.

[0021] Furthermore, a heating plate and a fan condenser are installed inside the shaker above the placement plate.

[0022] Furthermore, the shaker top is equipped with a movable shaker cover; the outer surface of the shaker is equipped with an integrated display screen.

[0023] Compared with the prior art, the advantages of this utility model are:

[0024] 1. Reduced risk of contamination: It eliminates the need for repeated sampling to measure bacterial concentration, fundamentally reducing experimental contamination caused by sampling for bacterial concentration measurement and improving the reliability of experimental results;

[0025] 2. Simplified Operation Procedures and Real-time Monitoring: The procedures for measuring bacterial concentration are significantly simplified, reducing experimental time. Simultaneously, the bacterial concentration value can be monitored in real-time via a display screen on the outside of the shaker, allowing for more precise control of experimental conditions and reducing the likelihood of subsequent experimental failures due to excessively high bacterial concentrations, thus improving the accuracy and stability of the experiment. Attached Figure Description

[0026] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0027] Appendix Fig. 1 This is a schematic diagram of the structure of an embodiment of this application;

[0028] Appendix Fig. 2 This is a schematic diagram of the main cross-sectional structure of an embodiment of this application;

[0029] Appendix Fig. 3 This is a schematic diagram of the left sectional view of an embodiment of this application;

[0030] Appendix Fig. 4 This is a schematic diagram of the internal structure of the grating monochromator according to an embodiment of this application;

[0031] Appendix Fig. 5 This is a schematic diagram of the oscillation component structure according to an embodiment of this application;

[0032] Appendix Fig. 6 This is a schematic cross-sectional view of the triangular bottle according to an embodiment of this application;

[0033] Appendix Fig. 7 This is a schematic diagram of the partition assembly structure according to an embodiment of this application.

[0034] Explanation of reference numerals and components in the accompanying drawings:

[0035] 1. Erlenmeyer flask; 11. Cuvette; 2. Shaker; 21. Placement plate; 22. Placement seat; 23. Iron frame; 24. Shaker movable cover; 3. Ultraviolet bacterial concentration measurement component; 31. Light source; 32. Grating monochromator; 321. Entrance slit; 322. Exit slit; 323. Collimating lens; 324. Grating; 325. Motor-controlled telescopic rod; 326. Focusing mirror; 33. Detector; 4. Oscillation component; 41. Movable base; 42. Trapezoidal iron plate; 43. Compression spring; 44. Oval iron block; 45. Threaded shaft; 46. Rotating motor; 5. Lamp source iron frame; 6. Display screen; 7. Partition assembly; 71. First partition; 72. Second partition; 73. Peg; 8. Heating plate; 9. Fan condenser. Detailed Implementation

[0036] The technical solution of this utility model will now be clearly and completely described through specific embodiments. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0037] See appendix Figs. 1-7 As shown, this application discloses a culture device for real-time measurement of bacterial concentration, comprising an Erlenmeyer flask 1, a shaker 2, an ultraviolet bacterial concentration measurement component 3, and an oscillation component 4. A cuvette 11 is installed at the bottom of the Erlenmeyer flask 1. The shaker 2 has an internal space for accommodating the Erlenmeyer flask 1, which is placed inside the shaker 2 for bacterial culture. A placement plate 21 and a placement seat 22 are installed inside the shaker 2. The placement plate 21 has a through hole along its thickness direction. The placement seat 22 is located below the placement plate 21 and cooperates with the through hole. An iron frame 23 is installed above the placement plate 21. The Erlenmeyer flask 1 is mounted on the placement plate 21 through the iron frame 23. The cuvette 11 is placed inside the placement seat 22 through the through hole. The ultraviolet bacterial concentration measurement component 3 is located inside the shaker 2 and measures the bacterial concentration of the Erlenmeyer flask 1. The oscillation component 4 is located inside the shaker 2 and is connected to the placement plate 21 to realize the oscillation culture of the Erlenmeyer flask 1 on the placement plate 21.

[0038] The above structure is further described below:

[0039] See appendix Figs. 1-7 As shown, the bottom of the Erlenmeyer flask 1 is equipped with a cuvette 11 for storing the liquid culture medium used in bacterial culture. The cuvette 11 is integrally molded or detachably connected to the bottom of the Erlenmeyer flask 1 to ensure sealing and stability. During bacterial culture, the cuvette 11 can be directly used for bacterial concentration measurement without additional sampling, effectively reducing the risk of contamination. The shaker 2 contains a placement plate 21 and a placement seat 22. An iron frame 23 is mounted on top of the placement plate 21, and the top of the iron frame 23 has a groove with an embedded spring for securely holding the Erlenmeyer flask 1. The iron frame 23 has an opening at the center of its bottom, and the placement plate 21 has a through hole along its thickness direction. The bottom of the placement plate 21 is connected to a placement seat 22, which also has a hole. The diameter of the opening at the bottom of the iron frame 23, the through hole on the placement plate 21, and the hole on the placement seat 22 are all adapted to the size of the cuvette 11. The cuvette 11 is placed into the placement seat 22 through the opening at the bottom of the iron frame 23, the through hole on the placement plate 21, and the hole on the placement seat 22 in sequence. This ensures that the triangular flask 1 remains stable during measurement and oscillation, and avoids measurement errors or liquid spillage due to shaking.

[0040] The shaker 2 is equipped with an ultraviolet (UV) bacterial concentration measurement component 3, which can measure the bacterial concentration of the Erlenmeyer flask 1 placed on the placement plate 21. This component includes a light source 31, a grating monochromator 32, an absorption cell, and a detector 33 located under the placement plate, and is also connected to a signal amplifier external to the shaker 2. A lamp source frame 5 is installed on the inner wall of the bottom of the shaker 2, and both the light source 31 and the grating monochromator 32 are mounted on the lamp source frame 5. The light source 31 is a halogen tungsten lamp, with its bottom vertically connected to the lamp source frame 5. The light propagation path of the halogen tungsten lamp is parallel to the placement plate 21. A power supply is connected to the side of the shaker 2, controlling the halogen tungsten lamp to emit light into the grating monochromator 32. The halogen tungsten lamp has advantages such as stable luminescence and a wide spectral range, providing stable and reliable light for bacterial concentration measurement. A grating monochromator 32 is positioned along the light propagation path of the halogen tungsten lamp. The grating monochromator 32 has a rectangular iron box structure. Openings are formed on both side walls along the light propagation direction. These openings serve as an entrance slit 321 for light to enter and an exit slit 322 for light to exit, respectively. Light propagates through the entrance slit 321 into the grating monochromator 32. Inside the grating monochromator 32, a collimating lens 323, made of copper, is installed along the light propagation path. The grating monochromator 32 has grooves on its inner sidewalls for equipping a collimating lens 323 to reflect the light entering it in parallel. A groove on the bottom wall of the grating monochromator 32 is for equipping a grating 324, which disperses the parallel light reflected by the collimating lens 323. Below the entrance slit 321, a motor-controlled telescopic rod 325 is mounted on the bottom wall of the grating monochromator 32 to control the angle between the grating 324 and the bottom of the grating monochromator 32, thus dispersing light of different wavelengths for detection. The dispersed light propagates to a focusing mirror 326 located inside the grating monochromator 32. The grating monochromator 32 has grooves on its unopened inner sidewalls for equipping the focusing mirror 326, which is perpendicular to the bottom of the grating monochromator 32 to focus the light dispersed by the grating 324 to the desired wavelength. Finally, the light enters the cuvette 11 through the exit slit 322. The above structure can accurately process light to meet the needs of detection of different wavelengths of light, so as to adapt to the requirements of measuring different bacterial concentrations.

[0041] A detector 33, i.e., a diode signal receiver, is installed on the inner wall of the shaker 2 to receive light passing through the cuvette 11. An absorption cell is also installed inside the shaker 2 to absorb light not introduced into the cuvette 11. The diode signal receiver is connected to a display screen 6 located on the surface of the shaker 2. The received signal is amplified and then displayed on the display screen 6 to show the desired bacterial concentration value. This application significantly simplifies the operational procedures for measuring bacterial concentration, reducing experimental time. Simultaneously, the bacterial concentration value can be monitored in real time via the display screen outside the shaker, allowing for more precise control of experimental conditions, reducing the likelihood of subsequent experimental failures due to excessively high bacterial concentrations, and improving the accuracy and stability of the experiment.

[0042] The oscillation assembly 4 includes a movable base 41, a trapezoidal iron plate 42, and a compression spring 43. The movable base 41 is set on the bottom inner wall of the shaker 2. An elliptical iron block 44 is installed on the movable base 41. A threaded shaft 45 is connected in the middle of the movable base 41. The threaded shaft 45 is also connected to the rotary motor 46. The elliptical iron block 44 is also connected to the rotary motor 46. The rotary motor 46 controls the elliptical iron block 44 to swing and controls the movable base 41 to move left and right. The rotary motor 46 can flexibly control the movement of the movable base 41, providing a power basis for subsequent oscillation operations. The placement plate 21 includes a first placement plate, a second placement plate, and a third placement plate. The first and second placement plates are symmetrically arranged on the inner walls of both sides inside the shaker 2. The third placement plate is movably connected to the first and second placement plates and is fixedly connected to the placement seat 22 below which the cuvette 11 is placed. The third placement plate is connected to a trapezoidal iron plate 42, the other end of which is connected to a movable base 41. The trapezoidal iron plate 42 interacts with an elliptical iron block 44 on the movable base 41, thereby controlling the rotation speed of the shaker 2. Simultaneously, the trapezoidal iron block 42 is connected to a compression spring 43, the bottom of which is connected to the inner wall of the shaker 2. The compression spring 43, through its interaction with the elliptical iron block 42, undergoes elastic deformation, causing the placement plate 21 to move left and right. Through the cooperation between the trapezoidal iron plate 42, the compression spring 43, and the elliptical iron block 44, stable oscillation of the placement plate 21 is achieved, meeting the oscillation requirements during bacterial culture. The oscillation assembly makes the Erlenmeyer flask more stable during oscillation, promoting full contact between bacteria and the culture medium, which is beneficial to bacterial growth and metabolism.

[0043] Meanwhile, a partition assembly 7 is installed inside the shaker 2. The partition assembly 7 includes a first partition 71, a second partition 72, and a bolt 73. The first partition 71 and the second partition 72 are cross-connected, and the bolt 73 is installed at the connection point. Both ends of the first partition 71 and the second partition 72 have openings along their length direction. The openings of the first partition 71 and the second partition 72 are connected to the placement plate 21 to reduce friction between them during oscillation and prevent damage caused by friction. The first partition 71 and the second partition 72 can rotate on the bolt 73.

[0044] A heating plate 8 and a fan condenser 9 are installed inside the shaker 2, located above the placement plate 21. Both the heating plate 8 and the fan condenser 9 are electrically connected to the display screen 6. The experimenter can adjust the working status of the heating plate 8 through the display screen 6, raising or lowering the internal temperature of the shaker 2 according to the temperature requirements for bacterial culture. Simultaneously, the fan condenser 9 is controlled via the display screen 6 to regulate the ventilation inside the shaker 2, maintaining air circulation and providing a suitable environment for bacterial culture. This application, through the cooperation of the heating plate and the fan condenser, can precisely control the internal temperature and ventilation conditions of the shaker, providing a suitable environment for bacterial culture, meeting the growth needs of different bacteria, and improving the culture effect.

[0045] The aforementioned display screen 6 is connected to the controller, and is also electrically connected to the halogen tungsten lamp, the motor-controlled telescopic rod 325, and the rotating motor 46. That is, the display screen 6 controls the on / off state and the light intensity of the halogen tungsten lamp to meet the light requirements for measuring different bacterial concentrations. The display screen 6 can also control the motor-controlled telescopic rod 325 to precisely adjust the angle between the bottom of the grating 324 and the grating monochromator 32, thereby dispersing light of accurate wavelengths for detecting bacterial concentrations. In addition, the display screen 6 can control the rotating motor 46 to adjust the swing amplitude and frequency of the elliptical iron block 44, thereby changing the oscillation frequency and amplitude of the placement plate 21 to meet the oscillation requirements in different bacterial culture processes.

[0046] For a better option, see the appendix. Fig. 1 As shown, the shaker 2 in this embodiment is provided with a movable shaker cover 24.

[0047] In practical use, the shaker cover 24 is first opened, and the triangular flask 1 containing liquid culture medium is placed on the placement plate 21 and fixed with the iron frame 23. The cuvette 11 is then placed in the placement seat 22, and the shaker cover 24 is closed. The internal temperature and ventilation conditions of the shaker 2 are then set via the display screen 6 by adjusting the internal heating plate 8 and fan condenser 9. Subsequently, the halogen tungsten lamp emits light via the display screen 6, which enters the grating monochromator 32. The motor controls the telescopic rod 325 via the display screen 6 to control the angle between the grating 324 and the bottom of the grating monochromator 32, thus dispersing light of different wavelengths to detect bacterial concentration. The measured bacterial concentration is displayed in real time on the display screen 6. During the culture process, the display screen 6 can control the rotating motor 46 to control the elliptical iron block 44 to swing. The mutual compression of the elliptical iron block 44 and the trapezoidal iron plate 42 pushes the compression spring 43 to oscillate. The oscillation frequency can be adjusted by controlling the movement of the movable base 41 to change the mutual compression position of the elliptical iron block 44 and the trapezoidal iron plate 42. This application integrates the light source 31, grating monochromator 32, detector 33, etc. into one unit, enabling control of bacterial concentration measurement via the display screen 6 outside the shaker 2. This eliminates the need for complex sample processing steps, significantly simplifying the operation process. This not only reduces the workload for experimenters but also saves considerable experimental time, allowing for highly efficient experiments.

[0048] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A culture device capable of measuring concentration of bacteria in real time, characterized by comprising: The utility model relates to a bacterial culture device, including: a triangular bottle with a colorimetric cup installed at the bottom; a shaking table with an accommodating space inside, the triangular bottle is set in the shaking table for bacterial culture, a placing plate and a placing seat are installed inside the shaking table, the placing plate is provided with a through hole along the thickness direction, the placing seat is below the placing plate and cooperates with the through hole, an iron stand is installed above the placing plate, the triangular bottle is installed on the placing plate through the iron stand, the colorimetric cup is placed in the placing seat through the through hole; a UV bacterial concentration measuring assembly is arranged inside the shaking table and measures the bacterial concentration of the triangular bottle; a shaking assembly is arranged inside the shaking table and connected with the placing plate to realize the shaking culture of the triangular bottle on the placing plate.

2. The culture device capable of measuring concentration of bacteria in real time according to claim 1, wherein, The UV bacterial concentration measuring assembly includes a light source, a grating monochromator, an absorption cell and a detector, a light source iron stand is installed on the inner wall of the bottom of the shaking table, the light source and the grating monochromator are installed on the light source iron stand, the grating monochromator cooperates with the light source, the grating monochromator guides the light emitted by the light source into the colorimetric cup, the absorption cell is installed inside the shaking table, the absorption cell absorbs the light not guided into the colorimetric cup, and the detector is installed on the inner side wall of the shaking table, and the detector receives the light passing through the colorimetric cup.

3. The culture device capable of measuring concentration of bacteria in real time according to claim 2, wherein, The grating monochromator is a rectangular iron box structure, openings are formed in the two side walls along the light propagation direction, and the two openings are respectively used as an entrance mirror slit for light incidence and an exit slit for emitted light; the light propagates into the grating monochromator through the entrance mirror slit, a collimating lens is installed in the grating monochromator along the light propagation path, the collimating lens reflects the light to a grating arranged inside the grating monochromator, the grating disperses the light, the dispersed light propagates to a focusing mirror arranged inside the grating monochromator, and the light is guided out of the exit slit after focusing through the focusing mirror.

4. The culture device capable of measuring concentration of bacteria in real time according to claim 3, wherein, A motor-controlled telescopic rod is installed below the entrance mirror slit, and the motor-controlled telescopic rod is connected with the grating.

5. The culture device capable of measuring concentration of bacteria in real time according to claim 1, wherein, The shaking assembly includes a movable base, a trapezoidal iron plate and a compression spring, the movable base is movably connected with the bottom inner wall of the shaking table, one end of the trapezoidal iron plate is connected with the placing plate, and the other end is connected with the movable base, one end of the compression spring is connected with the inner wall of the shaking table, and the other end is connected with the trapezoidal iron plate.

6. The culture device capable of measuring concentration of bacteria in real time according to claim 5, wherein, The movable base is connected with a rotating motor through a threaded shaft, the rotating motor drives the movable motor to make reciprocating motion in the shaking table, an elliptical iron block is installed on the movable base, the elliptical iron block is in transmission connection with the rotating motor, and a rotating motor controller controls the elliptical iron block to swing on the movable base.

7. The culture device capable of measuring concentration of bacteria in real time according to claim 1, wherein, A partition assembly is installed inside the shaking table, the partition assembly includes a first partition, a second partition and a peg, the first partition and the second partition are cross-connected, the peg is installed at the connection position, and openings are formed at both ends of the first partition and the second partition along the length direction.

8. The culture device capable of measuring concentration of bacteria in real time according to claim 1, wherein, The placing plate comprises a first placing plate, a second placing plate and a third placing plate, the first placing plate and the second placing plate are symmetrically arranged on the inner walls of the two sides of the inside of the rocking bed, and the third placing plate is movably connected with the first placing plate and the second placing plate.

9. The culture device capable of measuring concentration of bacteria in real time according to claim 1, wherein, A heating plate and a fan condenser are installed above the placing plate in the inside of the rocking bed.

10. The culture device capable of measuring concentration of bacteria in real time according to claim 1, wherein, The upper cover of the rocking bed is provided with a rocking bed movable cover, and the outer surface of the rocking bed is provided with an integrated display screen.

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