Multi-chamber cell culture device

Abstract

The utility model discloses a multi-chamber cell culture device, which belongs to the technical field of cell culture and comprises a base with an open top surface, a plurality of partition plates are detachably connected in the base, and the inner space of the base is divided into a plurality of co-culture chambers by the partition plates. One side of the co-culture chamber is fixedly connected with a placement frame with an open top surface and an open bottom surface, a plurality of culture tubes are placed on the placement frame, the bottom ends of the culture tubes are higher than the bottom surface of the co-culture chamber, and the bottom surfaces of the culture tubes are fixedly connected with semi-permeable membranes. According to the multi-cavity cell culture device, cell co-culture of the multiple culture tubes in the single co-culture chamber is achieved through the placement frame, the culture tubes and the semi-permeable membranes, mutual independence or communication of the multiple co-culture chambers is achieved through the detachable partition plates, and therefore joint co-culture of the multiple co-culture chambers is achieved; the problem that only two types of cells can be co-cultured is avoided, and the use flexibility and practicability are improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to cell culture technical field, especially relate to a multi -chamber cell culture device. BACKGROUND

[0002] Cell culture is an important technology in the field of biology and medicine, and the culture of single cells is the most widely used culture form. This culture method has the following advantages: direct observation of the morphological structure and life activities of living cells, study of gene and protein expression and changes and mechanisms under the action of different treatment factors, easy provision of a large number of experimental objects with similar biological characteristics, and low cost. The disadvantages are the lack of interaction between tumor cells and tumor cells, tumor cells and normal cells, and cells and the surrounding matrix, which cannot realize the exchange between multiple cells, that is, cannot accurately simulate the real environment of cells in vivo, which is extremely important for cell growth, proliferation, migration and other biological characteristics.

[0003] The existing multi-chamber cell culture device is not convenient to use in co-culture, such as the cell co-culture device shown in the authorized publication CN207391440U, which comprises at least three culture holes, a culture plate and an upper cover. The above-mentioned patent can only co-culture cells according to the adjacent culture holes of the buffer area when in use. The existing non-direct culture system on the market includes the Millicell hanging chamber of the Millipore company and the Transwell co-culture system of the Corning company. These commercial co-culture systems are simple to operate and are widely used in research at present. The disadvantage of these systems is that they can only co-culture two kinds of cells and cannot realize the simultaneous co-culture of multiple cells. If more types of cell co-culture are required, the use is not flexible and the practicality is low.

[0004] Therefore, we propose a multi-chamber cell culture device to solve the above problems. Utility model content

[0005] The utility model aims at solving the problems of insufficient flexibility and low practicality in the prior art, and proposes a multi-chamber cell culture device.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme:

[0007] The utility model provides a kind of multi-chamber cell culture device, including the base of open top, the inside detachable connection of the base is equipped with multiple partitions, and the inside space of the base is separated into multiple co-culture chambers by partition, co-culture chamber side fixedly connected with the open top and bottom of open rack, the rack is placed with multiple culture tubes, and the culture tube bottom is higher than the co-culture chamber bottom, and the culture tube bottom is fixedly connected with semi-permeable membrane;

[0008] It also includes a top cover that fits the base, and the top cover has a water injection hole above the side of the co-culture chamber that is away from the rack.

[0009] Preferably, the co-culture chamber side away from the rack is fixedly connected with a water injection plate, the water injection plate has a through hole, and one end of the through hole communicates with the water injection hole, and the other end of the through hole communicates with the open bottom of the rack.

[0010] Preferably, the culture tube has a flange extending outward from the top end, the rack has multiple placement holes for placing the culture tubes, and the placement hole top end has two sides with auxiliary holes for easy retrieval.

[0011] Preferably, the base inner wall has multiple U-shaped sliding grooves for the partition to slide up and down, and the partition has a sealing gasket fixedly connected to the two sides and bottom end to fit the sliding groove.

[0012] Preferably, the top cover bottom surface is attached to the partition top surface.

[0013] Preferably, the water injection hole has a detachable sealing plug.

[0014] In summary, the technical effects and advantages of the utility model are as follows: the multi-chamber cell culture device realizes cell co-culture of multiple culture tubes in a single co-culture chamber through the rack, culture tube, and semi-permeable membrane, and realizes joint co-culture of multiple co-culture chambers through detachable partitions that are independent or connected, which avoids the problem of only being able to co-culture two types of cells compared to existing devices, and improves flexibility and practicality. BRIEF DESCRIPTION OF DRAWINGS

[0015] Fig. 1 It is a structural schematic diagram of the utility model;

[0016] Fig. 2 It is a cross-sectional front view of the utility model;

[0017] Fig. 3 It is a cross-sectional side view of the utility model after removing the top cover.

[0018] In the figure: 1, base; 2, partition; 3, co-culture chamber; 4, placing rack; 5, culture tube; 6, semi-permeable membrane; 7, top cover; 8, water injection hole; 9, water injection plate; 10, through hole; 11, flange; 12, placing hole; 13, auxiliary hole; 14, sliding slot; 15, sealing gasket; 16, sealing plug. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments.

[0020] Referring to Figs. 1-3 A multi-chamber cell culture device, comprising a base 1 with an open top, a plurality of partitions 2 are detachably connected inside the base 1, and the inside space of the base 1 is divided into a plurality of co-culture chambers 3 by the partitions 2, and a placing rack 4 with open top and bottom is fixedly connected on one side of the co-culture chamber 3, a plurality of culture tubes 5 are placed on the placing rack 4, and the bottom end of the culture tube 5 is higher than the bottom surface of the co-culture chamber 3, and a semi-permeable membrane 6 is fixedly connected to the bottom surface of the culture tube 5. The semi-permeable membrane 6 can be selected from 0.4um-1.0um polyester film, so as to realize the exchange of small molecules and ions while limiting the cells in the culture tube 5. If a tumor cell invasion experiment is to be performed, a 6um-12um polyester film can be selected.

[0021] It also comprises a top cover 7 matched with the base 1, and the top cover 7 is provided with a water injection hole 8 above the side of the co-culture chamber 3 away from the placing rack 4.

[0022] Referring to Figs. 1-3 The multi-chamber cell culture device, in use, first adds liquid culture medium into the co-culture chamber 3 until the height of the culture medium is higher than the bottom end of the placing rack 4, then inserts the culture tube 5 with cultured cells into the placing rack 4, at this time, the top cover 7 is buckled to the base 1, and the cell culture can be started, and the culture medium can be added into the co-culture chamber 3 through the water injection hole 8 during the culture process. At this time, each co-culture chamber 3 is independent of each other, and the multiple culture tubes 5 in a single co-culture chamber 3 realize co-culture.

[0023] When joint co-culture of multiple co-culture chambers 3 is needed, only the partitions 2 in the base 1 are removed, and the culture medium in the adjacent co-culture chambers 3 is mixed with each other, and the joint co-culture can be realized.

[0024] Referring to Figs. 1-3The water injection plate 9 is fixedly connected to the side of the co-culture chamber 3 away from the placement rack 4, and the water injection plate 9 is provided with a through hole 10, one end of the through hole 10 is communicated with the water injection hole 8, and the other end of the through hole 10 is communicated with the open bottom of the placement rack 4. Since the height of the nutrient solution needs to be higher than the bottom end of the culture tube 5, the water injection plate 9 is used to limit the culture medium, so as to avoid waste of the nutrient solution. As shown in the drawings, two water injection plates 9 can be directly selected and fixedly connected to the two sides of the co-culture chamber 3, and the gap between the two water injection plates 9 can be regarded as the through hole 10. The advantage of this scheme is that the gap is designed to facilitate the taking and replacing of the partition plate 2.

[0025] The culture tube 5 is provided with a flange 11 extending outwardly at the top end, and the placement rack 4 is provided with a plurality of placement holes 12 for placing the culture tube 5. The flange 11 is designed to fix the culture tube 5 in the placement hole 12. In order to avoid the problem that the flange 11 at the bottom of the culture tube 5 completely adheres to the top surface of the placement rack 4, causing difficulty in taking the culture tube 5, the auxiliary hole 13 is provided at the top end of the placement hole 12.

[0026] Referring to Figs. 1-3 The inner wall of the base 1 is provided with a plurality of U-shaped sliding grooves 14 for the up-and-down sliding of the partition plate 2, and the partition plate 2 is fixedly connected with a sealing gasket 15 on both sides and the bottom end, which cooperates with the sliding groove 14. The sealing gasket 15 should be made of a material with elasticity and stable properties, such as silicone for sealing.

[0027] The top cover 7 is attached to the top surface of the partition plate 2, so that when the top cover 7 is closed, the top cover 7 can limit the partition plate 2, avoid the partition plate 2 from accidentally loosening and falling off, and cause the culture media of the two co-culture chambers 3 to be mixed accidentally, thereby improving the stability of the device.

[0028] The water injection hole 8 is detachably provided with a sealing plug 16, which avoids the interference of the external environment with the cells in the culture tube 5.

[0029] The above is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art can make equivalent replacement or change according to the technical scheme and the inventive concept of the present application within the technical range disclosed by the present application, which should be covered within the protection scope of the present application.

Claims

1. A multi-chamber cell culture device comprising a base (1) with an open top, characterized in that, The base (1) is detachably connected with a plurality of partitions (2) inside, and the inside space of the base (1) is divided into a plurality of co-culture chambers (3) by the partitions (2), one side of the co-culture chamber (3) is fixedly connected with a placing rack (4) with open top surface and bottom surface, a plurality of culture tubes (5) are placed on the placing rack (4), and the bottom end of the culture tube (5) is higher than the bottom surface of the co-culture chamber (3), and the bottom surface of the culture tube (5) is fixedly connected with a semi-permeable membrane (6); A top cover (7) matched with the base (1) is further included, and the top surface of the top cover (7) is provided with a water injection hole (8) above the side of the co-culture chamber (3) away from the placing rack (4).

2. The multi-chamber cell culture device of claim 1, wherein, The co-culture chamber (3) is fixedly connected with a water injection plate (9) on the side away from the placing rack (4), the water injection plate (9) is provided with a through hole (10), one end of the through hole (10) is communicated with the water injection hole (8), and the other end of the through hole (10) is communicated with the open bottom of the placing rack (4).

3. The multi-chamber cell culture device of claim 1, wherein, The culture tube (5) is outwardly extended with a flange (11) at the top end, the placing rack (4) is provided with a plurality of placing holes (12) for placing the culture tubes (5), and the placing holes (12) are provided with auxiliary holes (13) on both sides of the top end for convenient taking.

4. The multi-chamber cell culture device of claim 1, wherein, The inner wall of the base (1) is provided with a plurality of U-shaped sliding grooves (14) for the up-and-down sliding of the partitions (2), and the two sides and the bottom end of the partition (2) are fixedly connected with sealing pads (15) matched with the sliding grooves (14).

5. A multi-chamber cell culture device according to claim 4, wherein, The bottom surface of the top cover (7) is attached to the top surface of the partition (2).

6. The multi-chamber cell culture device of claim 1, wherein, A sealing plug (16) is detachably matched on the water injection hole (8).

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