Dry-type fluorescence immunoassay analyzer detection circuit

Abstract

The utility model provides a dry-type fluorescence immunoassay analyzer detection circuit, which comprises: a first circuit board, which comprises an ultraviolet lamp driving circuit, and after the ultraviolet lamp driving circuit is electrified, a light source is lightened, so that rays emitted by the light source can irradiate a reagent strip; a second circuit board; the first circuit board and the second circuit board are arranged separately; the second circuit board comprises a photodiode, a signal processing circuit and an AD conversion circuit, wherein the photodiode is used for converting a fluorescence signal reflected by the reagent strip into a voltage signal; the signal processing circuit is used for amplifying the voltage signal; and the AD conversion circuit is used for converting the amplified voltage signal into a digital signal. In the technical scheme provided by the invention, the first circuit board and the second circuit board are separately arranged, so that the instantaneous fluctuation of the light source switch does not interfere with the subsequent fluorescence signal processing, and the signal stability of the detection circuit is improved.

Description

Technical Field

[0001] This utility model relates to the field of circuit technology, and more specifically, to a detection circuit for a dry fluorescence immunoassay analyzer. Background Technology

[0002] The high-precision fluorescence detection circuit is mainly used in dry fluorescence immunoassay analyzers. It is used to detect the intensity of the fluorescence signal on the reagent strip. The circuit generates fluorescence by controlling the light source to irradiate the reagent on the reagent strip to generate fluorescence. The fluorescence signal is then sensed by a photodiode. The signal is processed by the circuit and converted from analog to digital. The intensity of the fluorescence signal is then calculated by the software algorithm. The concentration of the substance contained in the analyte can be calculated from the intensity of the signal.

[0003] However, the fluctuations generated at the moment the light source is switched on interfere with the subsequent processing of the fluorescence signal, resulting in poor signal stability of the existing fluorescence detection circuit. Summary of the Invention

[0004] In view of this, the purpose of this application is to provide a detection circuit for a dry fluorescence immunoassay analyzer that can improve the problem of poor signal stability in existing fluorescence detection circuits.

[0005] To achieve the above technical objectives, the technical solution adopted in this application is as follows:

[0006] This application provides a detection circuit for a dry fluorescence immunoassay analyzer, including:

[0007] The first circuit board includes an ultraviolet lamp driving circuit. When the ultraviolet lamp driving circuit is powered on, it lights up the light source so that the rays emitted by the light source can irradiate the reagent strip.

[0008] Second circuit board;

[0009] The first circuit board and the second circuit board are arranged separately;

[0010] The second circuit board includes a photodiode, a signal processing circuit, and an AD conversion circuit, wherein:

[0011] The photodiode is used to convert the fluorescence signal reflected by the reagent strip into a voltage signal;

[0012] The signal processing circuit is used to amplify the voltage signal;

[0013] The AD conversion circuit is used to convert the amplified voltage signal into a digital signal.

[0014] Furthermore, the signal processing circuit includes a first ground wire, and the AD conversion circuit includes a second ground wire. The first ground wire is connected through a specified resistor, and the resistance value of the specified resistor is 0 ohms.

[0015] Furthermore, the signal processing circuit includes a variable resistor and a signal processing sub-circuit, with the variable resistor connected to the signal processing sub-circuit.

[0016] Furthermore, the AD conversion circuit includes a converter chip and a buck chip. The buck chip includes a buck input terminal and a buck output terminal. The converter chip includes a conversion input terminal. The buck input terminal is connected to a power supply. The buck output terminal is connected to the conversion input terminal.

[0017] Furthermore, the detection circuit of the dry fluorescence immunoassay analyzer also includes a signal transmission interface circuit. The signal transmission interface circuit includes a connector. The input end of the connector is connected to a power supply. The connector includes a first output end, a second output end, and a third output end. The first output end is connected to the ultraviolet lamp driving circuit, the second output end is connected to the signal processing circuit, and the third output end is connected to the AD conversion circuit.

[0018] Furthermore, the ultraviolet lamp driving circuit includes a voltage regulator chip, a first inductor, a first resistor, a first capacitor, and a second capacitor;

[0019] The first output terminal of the voltage regulator chip is connected to the light source through the first inductor, and one end of the first resistor is connected to the light source, while the other end is grounded.

[0020] The first capacitor and the second capacitor are arranged in parallel, and both ends of the first capacitor and the second capacitor are respectively connected to the power supply line and the ground line. The power supply line is connected to the power input terminal of the voltage regulator chip, and one end of the ground line is grounded, while the other end is connected to the second output terminal of the voltage regulator chip.

[0021] Furthermore, the signal processing sub-circuit includes a first diode, a second diode, a second resistor, a third resistor, a fourth resistor, a fifth resistor, a sixth resistor, a fourth capacitor, a fifth capacitor, and a sixth capacitor;

[0022] The photodiode is connected to the input terminal of the signal processing sub-circuit;

[0023] The two ends of the first diode are connected to a photodiode and a second diode, respectively;

[0024] The two ends of the fourth resistor are respectively connected to the first diode and the second diode;

[0025] The two ends of the second resistor are connected to the input terminals of the first diode and the fourth resistor, respectively;

[0026] One end of the fifth resistor is grounded, and the other end is connected to the second diode;

[0027] One end of the third resistor is connected between the photodiode and the first diode, and the other end is connected between the fourth resistor and the second diode through the fourth capacitor. The third resistor is arranged in parallel with the first diode.

[0028] One end of the fifth capacitor is connected between the photodiode and the first diode, and the other end is connected between the third resistor and the fourth capacitor;

[0029] The sixth capacitor is connected in parallel with the second diode. One end of the sixth capacitor is connected between the sixth capacitor and the second diode, and the other end is connected between the second diode and the output terminal of the signal processing sub-circuit.

[0030] The sixth resistor, the sixth capacitor, and the second diode are all connected in parallel. One end of the sixth resistor is connected to a variable resistor, and the other end is connected between the second diode and the output terminal of the signal processing sub-circuit.

[0031] Furthermore, the AD conversion circuit also includes a seventh resistor and a second inductor;

[0032] The seventh resistor is connected to the signal input terminal of the converter chip through the second inductor, so that the voltage signal flows to the signal input terminal of the converter chip after passing through the seventh resistor and the second inductor.

[0033] The utility model adopting the above technical solution has the following advantages:

[0034] In the technical solution provided in this application, the first circuit board and the second circuit board are arranged separately so that the instantaneous fluctuation of the light source switch will not interfere with the subsequent processing of the fluorescence signal, thereby improving the stability of the detection circuit signal.

[0035] In the technical solution of this application, the ground wire of the signal processing circuit and the ground wire of the AD conversion circuit are connected through a specified resistor of 0 ohms, so as to isolate the ground wire of the analog signal and the ground wire of the digital signal and avoid signal interference on the ground wire. Attached Figure Description

[0036] This application can be further illustrated by the non-limiting embodiments given in the accompanying drawings. It should be understood that the following drawings only illustrate some embodiments of this application and should not be considered as limiting the scope. For those skilled in the art, other related drawings can be obtained from these drawings without any inventive effort.

[0037] Figure 1This is a schematic diagram of the structure provided for an embodiment of this application.

[0038] Figure 2 A schematic diagram of the fluorescence detection principle provided in the embodiments of this application.

[0039] Figure 3 This is a structural diagram of the ultraviolet lamp driving circuit provided in an embodiment of this application.

[0040] Figure 4 This is a structural diagram of a signal processing circuit provided in an embodiment of this application.

[0041] Figure 5 This is a schematic diagram of the AD conversion circuit provided in an embodiment of this application.

[0042] Figure 6 This is a circuit diagram of the signal transmission interface.

[0043] Figure 7 This is a schematic diagram showing the connection method between the first grounding wire, the second grounding wire, and the specified resistor.

[0044] Icons: 1-First circuit board; 101-UV lamp driver circuit; 1011-Light source; 1012-Power input terminal; 1013-Voltage regulator chip; 1014-First inductor; 1015-First resistor; 1016-First capacitor; 1017-Second capacitor; 1018-Third capacitor;

[0045] 2-Second circuit board; 201-Signal processing circuit; 2011-Variable resistor; 20111-Output terminal;

[0046] 2012 - Signal processing sub-circuit; 20121 - Input terminal; 20122 - First diode; 20123 - Second diode; 20124 - Second resistor; 20125 - Third resistor; 20126 - Fourth resistor; 20127 - Fifth resistor; 20128 - Sixth resistor; 201209 - Fourth capacitor; 201210 - Fifth capacitor; 201211 - Sixth capacitor;

[0047] 2013 - First grounding wire;

[0048] 202 - AD conversion circuit; 2021 - step-down chip; 20211 - step-down output terminal; 20212 - input terminal; 2022 - converter chip; 20221 - conversion input terminal; 2023 - second ground wire; 2024 - seventh resistor; 2025 - second inductor.

[0049] 3-Photodiode;

[0050] 4-Signal transmission interface circuit; 401-Connector; 4011-First output terminal; 4012-Second output terminal; 4013-Third output terminal.

[0051] 5-Signal Acquisition, Calculation and Control Module

[0052] 6 - Specify the resistor. Detailed Implementation

[0053] The present application will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that similar or identical parts are referred to by the same reference numerals in the drawings or description. Implementations not shown or described in the drawings are forms known to those skilled in the art. In the description of this application, terms such as "first" and "second" are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0054] This embodiment proposes a detection circuit for a dry fluorescence immunoassay analyzer, such as... Figure 1 As shown, it includes a first circuit board 1, a second circuit board 2, a photodiode 3, and a signal transmission interface circuit 4.

[0055] The first circuit board 1 includes an ultraviolet lamp driving circuit 101, and the second circuit board 2 includes a signal processing circuit 201 and an AD conversion circuit 202, which are electrically connected.

[0056] The ultraviolet lamp driving circuit 101 is connected to the power supply through the signal transmission interface circuit 4. When the ultraviolet lamp driving circuit 101 is powered on, it can illuminate the light source 1011, which is a component of the ultraviolet lamp driving circuit 101. The ultraviolet lamp driving circuit 101 is mainly used to control the switching on and off of the light source 1011 and its brightness. For example, the brightness of the light source 1011 can be remotely controlled to adjust the intensity of the excitation light.

[0057] The signal processing circuit 201 is used to convert the photocurrent signal corresponding to the light source 1011 into a voltage signal;

[0058] The AD conversion circuit 202 is used to convert voltage signals into digital signals.

[0059] In this embodiment, the first circuit board 1 and the second circuit board 2 are arranged separately. For example, the first circuit board 1 and the second circuit board 2 are connected by welding. Both the first circuit board 1 and the second circuit board 2 are fixed on the optical path box, which is a box.

[0060] The first circuit board 1 is installed on the left side of the optical path box. Ultraviolet light is irradiated onto the reagent card through the lens, reflector and other devices installed in the optical path box.

[0061] The second circuit board 2 is installed above the optical path box. The fluorescence generated by the reagent card after being excited by ultraviolet light is irradiated onto the receiving photodiode through lenses and other devices inside the optical path box. Figure 2 As shown, the signal is processed by the second circuit board 2 and then transmitted to the signal acquisition, calculation and control module 5, whereby the fluorescence signal intensity is finally calculated.

[0062] Therefore, by arranging the first circuit board 1 and the second circuit board 2 separately, the interference of the instantaneous switching of the ultraviolet lamp driving circuit 101 on the fluorescence signal processing of the second circuit board 2 is reduced.

[0063] like Figure 3 and Figure 6 As shown, the signal transmission interface circuit 4 includes a connector 401. Pins 1 and 3 of the connector 401 are used as pins connected to the power supply, and pin 10 is the first output terminal 4011. The first output terminal 4011 is connected to the power input terminal 1012, and then the voltage of the power supply is input to pin 2 of the voltage regulator chip 1013 of the ultraviolet lamp driver circuit 101 and output from pin 5. In this embodiment, the output voltage of pin 5 is modulated by PWM so that the light source 1011 can output light intensity to meet different requirements. In this embodiment, the light output by the light source 1011 shines on the reagent strip.

[0064] In this embodiment, the light source 1011 is an LED lamp capable of emitting purple light.

[0065] like Figure 4 and Figure 6 As shown, the signal processing circuit 201 includes a variable resistor 2011 and a signal processing sub-circuit 2012. The signal processing sub-circuit 2012 is connected to the photodiode 3. The photodiode 3 is located at the beginning of the signal processing sub-circuit 2012. The photodiode 3 is used to receive the fluorescence reflected by the reagent strip. The light signal corresponding to the fluorescence is input to the photodiode 3, and the photodiode 3 converts the light signal into a voltage signal and outputs it.

[0066] The variable resistor 2011 can adjust its resistance under the control of external software. For example, the variable resistor 2011 can be an adjustable digital potentiometer chip AD5272.

[0067] like Figure 4 As shown, the variable resistor 2011 includes an output terminal 20111, which is connected to the input terminal 20121 on the signal processing sub-circuit 2012. This allows the variable resistor 2011 to amplify or reduce the voltage signal and output it after adjusting its resistance value.

[0068] In this embodiment, the voltage signal is generated by photodiode 3, processed by signal processing sub-circuit 2012 and variable resistor 2011, and then output by the attached... Figure 4 The output terminal VOUT is used for output.

[0069] In this embodiment, Figure 4 The I2C SCL wiring and I2C SDA wiring are in Figure 6 The second output terminal 4012 of the connector 401 is connected to the second output terminal 4012, which is connected to pins 9 and 11 of the chip of the signal transmission interface circuit 4.

[0070] like Figure 5 and Figure 6 As shown, the AD conversion circuit 202 includes a step-down chip 2021 and a converter chip 2022. The converter chip 2022 is used to receive voltage signals and convert the voltage signals into digital signals, which are then output to the signal acquisition, calculation and control module 5. The signal acquisition, calculation and control module 5 is used to calculate the intensity of the fluorescence signal based on the collected digital signals.

[0071] In this embodiment, the voltage signal is transmitted through... Figure 5 The VOUT input terminal is input to the converter chip 2022. The converter chip 2022 processes the voltage signal into a digital signal and outputs it from the SCLK and SIDO output terminals.

[0072] In this embodiment, as Figure 5 and Figure 6 As shown, connector 4013 includes a third output terminal 4013, which can be an interface. The third output terminal 4013 is connected to the input terminal 20212 of buck chip 2021.

[0073] like Figure 4 , Figure 5 and Figure 7 As shown, the signal processing circuit 201 includes a first ground wire 2013, and the AD conversion circuit 202 includes a second ground wire 2023. The first ground wire 2013 is connected to the second ground wire 2023 through a specified resistor 6, the resistance of which is 0 ohms. The signal processing circuit 201 converts the fluorescent signal into an analog voltage signal, and the AD conversion circuit 202 converts the voltage signal into a digital signal. Therefore, this design separates the ground wires for the analog signal and the digital signal, connecting the first ground wire 2013 and the second ground wire 2023 through the specified 0-ohm resistor. This means that all the first ground wires 2013 and the second ground wire 2023 are ultimately connected to the same point. Single-point grounding ensures minimal potential difference between all ground wires, avoiding signal interference on the ground wires.

[0074] like Figures 2-6As shown, the signal flow in this embodiment is as follows: After the ultraviolet lamp driving circuit 101 is powered on, the light source 1011 emits light that shines onto the reagent strip. The fluorescence signal reflected by the reagent strip is received by the photodiode 3. The photodiode 3 processes the fluorescence signal into a voltage signal and transmits it to the signal processing circuit 201. After the voltage signal is modulated by the variable resistor 2011, the amplified voltage signal is passed through... Figure 4 The VOUT output terminal is passed to Figure 5 The VOUT input terminal, after passing through the converter chip 2022, the converter chip 2022 processes the voltage signal into a digital signal, and then transmits it out through the SCLK and SIDO wiring.

[0075] like Figure 3 As shown, the UV lamp driving circuit 1 also includes a voltage regulator chip 1013, a first inductor 1014, a first resistor 1015, a first capacitor 1016, a second capacitor 1017, and a third capacitor 1018.

[0076] The first output terminal of the voltage regulator chip 1013 is connected to the light source 1011 through the first inductor 1014. One end of the first resistor 1015 is connected to the light source 1011, and the other end is grounded. In this embodiment, the first output terminal can be... Figure 3 Pin 5 of the voltage regulator chip 1013 in this embodiment. The voltage regulator chip 1013 in this embodiment can be an LDO voltage regulator chip.

[0077] The first capacitor 1016 and the second capacitor 1017 are connected in parallel, and both ends of the first capacitor 1016 and the second capacitor 1017 are respectively connected to the power supply terminal and the ground terminal. The power supply terminal is connected to the power input terminal of the voltage regulator chip 1013, and one end of the ground terminal is grounded, while the other end is connected to the second output terminal of the voltage regulator chip 1013. In this embodiment, the power input terminal is pin 4 of the voltage regulator chip 1013, and the second output terminal is pin 3 of the voltage regulator chip 1013.

[0078] In this embodiment, one end of the third capacitor 1018 is connected to pin 6 of the voltage regulator chip 1013, and the other end is connected to the first inductor 1014.

[0079] like Figure 4 As shown, the signal processing sub-circuit 2012 includes a first diode 20122, a second diode 20123, a second resistor 20124, a third resistor 20125, a fourth resistor 20126, a fifth resistor 20127, a sixth resistor 20128, a fourth capacitor 201209, a fifth capacitor 201210, and a sixth capacitor 201213. Wherein:

[0080] Photodiode 3 is connected to the input terminal of signal processing sub-circuit 2012.

[0081] The two ends of the first diode 20122 are connected to the photodiode 3 and the fourth resistor 20126, respectively.

[0082] The end of the fourth resistor 20126 furthest from the first diode 20122 is connected to the second diode 20123.

[0083] The two ends of the second resistor 20124 are connected to the input terminals of the first diode 20122 and the fourth resistor 20126, respectively.

[0084] One end of the fifth resistor 20127 is grounded, and the other end is connected to the second diode 20123.

[0085] One end of the third resistor 20125 is connected between the photodiode 3 and the first diode 20122, and the other end is connected between the fourth resistor 20126 and the second diode 20123 through the fourth capacitor 201209. The third resistor 20125 and the first diode 20122 are arranged in parallel.

[0086] One end of the fifth capacitor 201210 is connected between the photodiode 3 and the first diode 20122, and the other end is connected between the third resistor 20125 and the fourth capacitor 20128.

[0087] The sixth capacitor 201213 is connected in parallel with the second diode 20123. One end of the sixth capacitor 201213 is connected between the fifth resistor 20127 and the second diode 20123, and the other end is connected between the second diode 20123 and the signal output terminal of the signal processing sub-circuit.

[0088] The sixth resistor 20128, the sixth capacitor 201213, and the second diode 20123 are all connected in parallel. One end of the sixth resistor 20128 is connected to the variable resistor 2011, and the other end is connected between the second diode 20123 and the signal output terminal of the signal processing sub-circuit 2012. The signal output terminal indicates the position where it is connected to the AD conversion circuit signal.

[0089] like Figure 5 As shown, the AD conversion circuit 202 also includes a seventh resistor 2024 and a second inductor 2025.

[0090] The seventh resistor 2024 is connected to the signal input terminal of the converter chip 2022 through the second inductor 2025, so that the voltage signal flows to the signal input terminal of the converter chip after passing through the seventh resistor 2024 and the second inductor 2025.

[0091] The above description is merely an embodiment of this application and is not intended to limit the scope of protection of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of protection of this application.

Claims

1. A detection circuit for a dry fluorescence immunoassay analyzer, characterized in that, The application relates to a dry-type fluorescence immunoassay analyzer detection circuit. The first circuit board comprises an ultraviolet lamp driving circuit, which is powered on to light a light source, so that the light source can emit rays to irradiate a reagent strip. The second circuit board comprises a photodiode, a signal processing circuit and an AD conversion circuit. The photodiode is used for converting a fluorescent signal reflected by the reagent strip into a voltage signal. The signal processing circuit is used for amplifying the voltage signal. The AD conversion circuit is used for converting the amplified voltage signal into a digital signal. The signal processing circuit comprises a first grounding line, and the AD conversion circuit comprises a second grounding line. The signal processing circuit comprises a variable resistor and a signal processing sub-circuit.

2. The dry fluorescence immunoassay analyzer detection circuit according to claim 1, characterized in that, The AD conversion circuit comprises a converter chip and a step-down chip.

3. The dry fluorescence immunoassay analyzer detection circuit according to claim 2, characterized in that, The signal transmission interface circuit comprises a connector.

4. The dry fluorescence immunoassay analyzer detection circuit according to claim 1, characterized by, The ultraviolet lamp driving circuit comprises a voltage stabilizing chip, a first inductor, a first resistor, a first capacitor and a second capacitor.

5. The dry fluorescence immunoassay analyzer detection circuit according to claim 2, characterized in that, The first output terminal of the voltage stabilizing chip is connected with the light source through the first inductor.

6. The dry fluorescence immunoassay analyzer detection circuit according to claim 3, wherein, The first capacitor and the second capacitor are connected in parallel. The signal processing sub-circuit comprises a first diode, a second diode, a second resistor, a third resistor, a fourth resistor, a fifth resistor, a sixth resistor, a fourth capacitor, a fifth capacitor and a sixth capacitor. The photodiode is connected with the input end of the signal processing sub-circuit.

7. The dry fluorescence immunoassay analyzer detection circuit according to claim 3, characterized in that, The two ends of the first diode are respectively connected with the photodiode and the second diode. The two ends of the fourth resistor are respectively connected with the first diode and the second diode. The two ends of the second resistor are respectively connected with the first diode and the input end of the fourth resistor. One end of the fifth resistor is grounded, and the other end is connected with the second diode. One end of the third resistor is connected between the photodiode and the first diode, and the other end is connected between the fourth resistor and the second diode through the fourth capacitor. The third resistor is connected in parallel with the first diode. ​ One end of the fifth capacitor is connected between the photodiode and the first diode, and the other end is connected between the third resistor and the fourth capacitor; The sixth capacitor is connected in parallel with the second diode, one end of the sixth capacitor is connected between the sixth capacitor and the second diode, and the other end is connected between the second diode and the output terminal of the signal processing sub-circuit; The sixth resistor is connected in parallel with the sixth capacitor and the second diode, one end of the sixth resistor is connected with the variable resistor, and the other end is connected between the second diode and the output terminal of the signal processing sub-circuit.

8. The dry fluorescence immunoassay analyzer detection circuit according to claim 4, characterized in that, The AD conversion circuit further comprises a seventh resistor and a second inductor; The seventh resistor is connected with the signal input terminal of the converter chip through the second inductor, so that the voltage signal flows to the signal input terminal of the converter chip through the seventh resistor and the second inductor.

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