QPCR (Quantitative Polymerase Chain Reaction) sealing plate film pasting device

Abstract

The utility model belongs to the technical field of biological pharmacy, and particularly relates to a QPCR (Quantitative Polymerase Chain Reaction) sealing plate film pasting device. Comprising a base, an L-shaped plate, a cover plate and a steel wire, the cover plate is used for placing a film, and the cover plate is rotationally connected with the base; the base is in sliding connection with the L-shaped plate; one end of the steel wire is fixedly connected with the L-shaped plate, and the other end of the steel wire is fixedly connected with the film of the film. According to the scheme, the problem that the QPCR plate is polluted due to the fact that dust in air easily pollutes the adhesive layer during film pasting is solved.

Description

Technical Field

[0001] This invention belongs to the field of biopharmaceutical technology, specifically relating to a qPCR sealing film application device. Background Technology

[0002] In the fields of biotechnology and biopharmaceuticals, qPCR (quantitative real-time PCR) amplification experiments often require sealing the qPCR plate after sample loading. During sealing, the sealing plate must be scraped along all outer edges of the wells at least twice to ensure a tight seal and prevent evaporation, leakage, and cross-contamination of the qPCR reaction solution. However, due to the material of the sealing film and the narrow gaps between wells, misalignment of the sealing film and incomplete scraping are common problems. Furthermore, repeated scraping is time-consuming, and these issues can negatively impact subsequent amplification results. Therefore, a method or device to solve these problems is needed. To this end, we propose a qPCR plate sealing device.

[0003] Currently, an existing QPCR plate laminating device (publication number CN220537789U) includes a base with a cover hinged to it. A detachable stainless steel 96-well plate is embedded in the base. The base and cover are connected by a pivot. A power socket is located at the rear of the base. A stainless steel heating plate is embedded in the cover. A handle is located at the front of the cover. A temperature adjustment knob is located on the cover. A temperature control device and a temperature display device are installed inside the cover. A power switch is located on the side of the cover. This QPCR plate laminating device has a simple structure, ingenious design, and reasonable layout, enabling it to quickly and efficiently press the sealing film onto a 96-well QPCR plate, making it quite practical.

[0004] However, there is also a problem: the solution requires peeling off the film first, and then rotating and pressing it down, which can easily cause dust to adhere to the adhesive layer, resulting in contamination of the QPCR plate. Utility Model Content

[0005] This solution provides a QPCR sealing film application device to address the problem that dust in the air can easily contaminate the adhesive layer during film application, leading to QPCR plate contamination.

[0006] This solution provides a qPCR sealing film application device, including:

[0007] Base; used to hold the QPCR plate;

[0008] Cover plate: used to place the film, the cover plate is rotatably connected to the base;

[0009] Its characteristic is that it further includes:

[0010] L-shaped plate: The base is slidably connected to the L-shaped plate;

[0011] Steel wire: One end of the steel wire is fixedly connected to the L-shaped plate, and the other end is fixedly connected to the film to which the film is applied.

[0012] The principle of this method is as follows: The operator places the qPCR plate on the base, then fixes the film onto the cover plate, aligning the two. A steel wire is then attached to the film. The operator closes the cover plate, ensuring the film adheres tightly to the qPCR plate. The base is then slid; as the base moves, the steel wire pulls the film off, exposing the adhesive layer, which then directly contacts and adheres to the qPCR plate. This process continues until the steel wire pulls the entire film out, completing the film application.

[0013] The advantages of this solution are: 1. The automated application process reduces manual operation time and improves the speed and efficiency of experimental preparation. 2. The adhesive layer makes rapid contact with the QPCR plate, making it difficult for dust in the air to adhere to the adhesive layer, thus ensuring its cleanliness.

[0014] Furthermore, it also includes a guide rail box with an inner groove that mates with a cover plate and a base. The cover plate and base are rotatably connected by a torque spring. The size of the inner groove is just right so that the cover plate and base can be inserted after they are closed. In the initial state, the cover plate is open under the action of the torque spring. After the operator places the film and QPCR board, they only need to push the base completely into the inner groove, and the cover plate will automatically close. When pushing further in, the steel wire begins to pull the film to peel it off.

[0015] After the film is removed, when you need to open the cover, simply pull out the base, and the cover will open automatically under the action of the torque spring.

[0016] This mechanism opens and closes the cover by sliding the base into and out of the inner groove. The cover is guided by the inner groove when closing, ensuring consistent closing position and force each time, thus guaranteeing consistent film application quality and reducing operational variations caused by human error. The operator simply pushes the cover inward to close it and then peel off the film, simplifying the process and improving efficiency.

[0017] Furthermore, it also includes a spring, a connecting rod, and a sliding block. One end of the connecting rod is fixedly connected to the base, and the other end is fixedly connected to the sliding block. The sliding block is slidably connected to the guide rail. One end of the spring is fixedly connected to the sliding block, and the other end is fixedly connected to the inner groove. The sliding block and connecting rod ensure that the base will not deviate in direction when pushed in. The spring automatically ejects the base after the cover is closed and the film is torn off, and automatically opens the cover when the base ejects. This design not only simplifies the operation process and reduces the number of steps the operator has to manually pull out the base, but also improves work efficiency.

[0018] Furthermore, it also includes a pressing mechanism, which comprises a pressing cylinder and a rolling frame. The rolling frame is fixedly connected to the L-shaped plate, and the pressing cylinder is rotatably connected to the rolling frame, with the pressing cylinder cooperating with the base. When the base is in its initial state, the roller is located on the left side of the base. After the operator places the QPCR plate into the base, they push the base into the inner groove. At this time, the pressing cylinder cleans the dust on the surface of the QPCR plate. When the pressing cylinder is completely removed from above the QPCR plate, the cover plate is also completely closed due to the action of the inner groove. Pushing the wire further in will then pull the film, thus tearing it off.

[0019] After the film is removed, the operator releases their hands, and the base slides out quickly under the action of the spring. The cover will also open, and at the same time, the pressure cylinder will press the film over the QPCR plate to compress and compact it.

[0020] Our system can automatically compact the film after application, reducing air bubbles and improving efficiency.

[0021] Furthermore, the base is provided with multiple positioning blocks, which are rectangularly distributed on the base. The positioning blocks use four-corner positioning to ensure that the QPCR plate will not shift during the film application process.

[0022] Furthermore, the cover plate is equipped with positioning pins for positioning the film application area. These positioning pins allow the operator to accurately place the film, ensuring alignment between the film and the QPCR plate.

[0023] Furthermore, it also includes a pull rope. The guide rail box has a through hole, one end of which is fixedly connected to the sliding block, and the other end passes through the through hole. The operator can pull the base and the sliding block by stretching the rope, avoiding the inconvenience of pushing by hand. Attached Figure Description

[0024] Figure 1 This is a structural diagram of a qPCR sealing film application device.

[0025] Figure 2 This is a diagram showing the open state of a qPCR sealing film application device.

[0026] The reference numerals in the accompanying drawings include: 1. Base; 2. Cover plate; 3. Rolling frame; 4. L-shaped plate; 5. Guide rail box; 6. Pull rope; 7. Connecting rod; 8. Steel wire; 9. Spring; 10. Sliding block; 11. Positioning bolt; 12. Pressure cylinder; 13. Positioning block. Detailed Implementation

[0027] As attached Figure 1 As shown:

[0028] This solution provides a QPCR sealing film application device, which includes a base 1, a cover plate 2, an L-shaped plate 4, a guide rail box 5, a steel wire 8, a spring 9, a sliding block 10, a rolling frame 3, a pressure cylinder 12, and a pull rope 6.

[0029] The L-shaped plate 4 is fixedly connected to the guide rail box 5. The base 1 slides on the inner groove of the L-shaped plate 4 and the guide rail box 5. The inner groove is flush with the horizontal plate of the L-shaped plate 4, so that the base 1 can slide from the L-shaped plate 4 into the inner groove.

[0030] The cover plate 2 and the base 1 are rotatably connected by a torque spring 9. The rolling frame 3 is fixed on the vertical plate of the L-shaped plate 4 and is a ring frame. The left end is rotatably connected to a pressure cylinder 12.

[0031] As attached Figure 1 . Figure 2 As shown:

[0032] The base 1 has four rectangularly distributed positioning blocks 13, sized to fit a 96-well plate. The QPCR plate will not shift position when placed within them. The pressure cylinder 12 is initially located at the left end of the base 1, allowing the operator to easily insert the QPCR plate from the center of the rolling frame 3. The pressure cylinder 12 is made of rubber and has a certain deformation capacity; as it passes back and forth over the QPCR plate, it compresses the plate. The cover plate 2 also has two equidistant positioning pins 11 to determine the placement position of the film.

[0033] One end of the connecting rod 7 is fixedly connected to the base 1, and the other end is fixedly connected to the sliding block 10. The sliding block 10 is slidably connected to the inner groove inside the guide rail box 5.

[0034] The guide rail box 5 has a through hole on its left end for one end of the pull rope 6 to pass through, and the other end of the pull rope 6 is fixedly connected to the sliding block 10. One end of the spring 9 is fixedly connected to the sliding block 10, and the other end is fixedly connected to the left end of the inner groove. The operator can pull the sliding block 10 by pulling the pull rope 6.

[0035] The steel wire 8 is in a relaxed state in the initial state. The steel wire 8 will only pull the membrane when the base 1 is fully inserted into the inner groove.

[0036] As attached Figure 1 , Figure 2 As shown:

[0037] The principle of this solution is as follows: In the initial state, the cover plate 2 is kept open by the torque spring 9, and the base 1 is located outside the inner groove of the guide rail box 5. The operator places the QPCR board on the base 1, and the four corners of the base 1 are equipped with positioning blocks 13 to ensure that the QPCR board is fixed in position. Then, the film is covered on the positioning bolts 11 of the cover plate 2, and the positioning bolts 11 are used to precisely align the film with the holes of the QPCR board.

[0038] The operator pulls the extended rope 6 of the guide rail box 5, and the sliding block 10 slides along the guide rail of the guide rail box 5, causing the base 1 to slide into the inner groove. When the base 1 is fully inserted into the inner groove of the guide rail box 5, the cover plate 2 is guided to close by the inner groove. At this time, the torque spring 9 is compressed, and the cover plate 2 and the base 1 are tightly fitted together.

[0039] As the base 1 continues to slide inward, the steel wire 8 fixed to the L-shaped plate 4 begins to pull the release layer of the film. One end of the steel wire 8 is fixedly connected to the edge of the film, while the other end generates tension as the L-shaped plate 4 slides, causing the release layer to peel off gradually, exposing the adhesive layer. During this process, the adhesive layer quickly comes into contact with the QPCR plate, preventing dust contamination from the air.

[0040] Once the film is completely peeled off, the operator releases the pull cord 6, the spring 9 releases its elasticity, and pushes the sliding block 10 to slide in the opposite direction along the guide rail, causing the base 1 to quickly return to its initial position. During the reset process, the cover plate 2 automatically opens under the action of the torque spring 9. At the same time, the pressure cylinder 12 on the rolling frame 3 rolls over the surface of the QPCR plate, pressing the film firmly, eliminating air bubbles, and ensuring a seal.

[0041] During the sliding of base 1, pressure cylinder 12 is located on the left side of base 1, initially cleaning the surface of the QPCR board by rolling to remove dust. After base 1 is fully pushed in, pressure cylinder 12 exits the QPCR board area to avoid interfering with the film application process.

[0042] The beneficial effects of this solution are as follows: 1. By automating the film application and compaction process, manual operation time is reduced, improving the speed and efficiency of experimental preparation. 2. The adhesive layer makes rapid contact with the QPCR plate, making it difficult for dust in the air to adhere to the adhesive layer, ensuring its cleanliness. 3. This mechanism opens and closes the cover plate 2 by sliding the base 1 into and out of the inner groove. The cover plate 2 is guided by the inner groove when closing, ensuring consistent closing position and force each time, thus ensuring consistent film application quality and reducing operational differences caused by human factors. 4. This mechanism can automatically perform the compaction work after film application, reducing the generation of air bubbles and improving efficiency.

[0043] The above descriptions are merely embodiments of this utility model, and common knowledge regarding specific structures and characteristics is not elaborated upon here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of this utility model, and these should also be considered within the scope of protection of this utility model. These modifications will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application shall be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A qPCR sealing film application device, comprising: Base (1); Used to fix qPCR plates; Cover plate (2): used to place the film, the cover plate (2) is rotatably connected to the base (1); Its characteristic is that it further includes: L-shaped plate (4): The base (1) is slidably connected to the L-shaped plate (4); Steel wire (8): One end of the steel wire (8) is fixedly connected to the L-shaped plate (4), and the other end is fixedly connected to the film.

2. The qPCR sealing film application device according to claim 1, characterized in that, It also includes a guide rail box (5), which has an inner groove that cooperates with the cover plate (2) and the base (1). The cover plate (2) and the base (1) are rotatably connected by a torque spring (9).

3. The qPCR sealing film application device according to claim 2, characterized in that, It also includes a spring (9), a connecting rod (7) and a sliding block (10). One end of the connecting rod (7) is fixedly connected to the base (1) and the other end is fixedly connected to the sliding block (10). The sliding block (10) is slidably connected to the guide rail. One end of the spring (9) is fixedly connected to the sliding block (10) and the other end is fixedly connected to the inner groove.

4. The qPCR sealing film application device according to claim 3, characterized in that, It also includes a pressing mechanism, which includes a pressing cylinder (12) and a rolling frame (3). The rolling frame (3) is fixedly connected to the L-shaped plate (4), and the pressing cylinder (12) is tumbled to the rolling frame (3). The pressing cylinder (12) also cooperates with the base (1).

5. The qPCR sealing film application device according to claim 1, characterized in that, The base (1) is provided with a plurality of positioning blocks (13), which are distributed in a rectangular shape on the base (1).

6. The qPCR sealing film application device according to claim 1, characterized in that, The cover plate (2) is provided with a positioning bolt (11), which is used to position the film application.

7. The qPCR sealing film application device according to claim 3, characterized in that, It also includes a pull rope (6), the guide rail box (5) is provided with a through hole, one end of the pull rope (6) is fixedly connected to the sliding block (10), and the other end passes through the through hole.

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