DNA watermark encryption storage rack for strain protection

Abstract

The utility model discloses a DNA watermark encryption storage rack for strain protection, and relates to the field of strain storage equipment, the DNA watermark encryption storage rack comprises a storage rack body, a box body is installed in the storage rack body, a DNA watermark encryption module is installed in the box body, an alarm assembly is installed at the top of the storage rack body, and the storage rack body is connected with the box body. The alarm assembly comprises a monitoring alarm system, a controller and an alarm, the monitoring alarm system is installed on the top of the storage rack body and connected with the DNA watermark encryption module, the controller is installed on the front face of the monitoring alarm system, and the alarm is installed on the top of the monitoring alarm system. According to the utility model, the DNA watermark encryption module gives specific DNA watermark information to the security lock and is bound with a storage position, when access is carried out, the operation is controlled by verifying the DNA watermark information and the authorization of the security lock system, and the alarm system is started in case of abnormity.

Description

Technical Field

[0001] This utility model relates to the field of microbial strain storage equipment, specifically a DNA watermark encryption storage rack for microbial strain protection. Background Technology

[0002] Against the backdrop of the booming development of modern biotechnology, the fermentation industry, as an important branch, relies on various microbial strains as core production factors. As living cell catalysts, microbial strains play an indispensable role in many industrial fields such as food, medicine, and chemicals. They encompass four main categories: bacteria, actinomycetes, yeasts, and molds, each possessing unique physiological and biochemical characteristics, providing diverse options for the fermentation production of different products. These strains initially originate from widely distributed microbial resources in nature. Researchers must expend considerable effort collecting samples from complex natural environments and employing advanced microbial isolation techniques to accurately identify and isolate target strains from numerous microorganisms. Subsequently, through a rigorous screening process, strains with excellent fermentation performance, high activity, and stability are selected. To further improve production efficiency, genetic engineering, mutagenesis breeding, and other improvement methods are used to optimize the strains. After this series of complex and time-consuming processes, the obtained high-quality strains are properly stored for subsequent large-scale production.

[0003] In the entire process of microbial strain management, storage is a crucial step in ensuring the quality and activity of the strains. Traditional methods of strain storage are diverse, including sealing in simple containers such as glass tubes and plastic centrifuge tubes, and then storing them in ordinary refrigerators or freezers. However, with the continuous advancement of biotechnology and the ongoing expansion of the industry, the requirements for strain storage are becoming increasingly stringent. On the one hand, traditional storage containers and equipment are gradually revealing limitations in terms of sealing, temperature stability, and humidity control, failing to provide a long-term, stable, and precise storage environment for the strains, easily leading to a decline in strain activity or even death. On the other hand, as the commercial value and scientific significance of microbial strains become increasingly prominent, the security threats they face are also growing daily. The risks of illegal acquisition, malicious tampering, or accidental operation leading to the loss or contamination of strains cannot be ignored, and traditional storage methods are inadequate in preventing these risks, lacking effective identification and security protection mechanisms.

[0004] Currently, although some patents involve improvements to microbial culture storage equipment, such as the microbial culture storage rack disclosed in patent document CN218791280U, which solves the problems of humidity control and cabinet sealing to some extent through innovative drawer structures and desiccant monitoring devices, existing storage rack technology still has significant shortcomings in terms of overall security. In particular, there are no effective solutions yet for preventing illegal acquisition of microbial cultures and for identifying and protecting microbial cultures at the genetic level.

[0005] This situation urgently requires the emergence of a new, highly safe, and intelligent microbial culture storage technology to meet the increasingly stringent demands of the modern fermentation industry for microbial culture storage and to ensure the safety, integrity, and traceability of microbial resources during the storage process. Utility Model Content

[0006] The purpose of this invention is to provide a DNA watermark-encrypted storage rack for strain protection, in order to solve the technical problems mentioned in the background art that strain storage racks cannot prevent illegal acquisition of strains and cannot identify and protect strains at the genetic level.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a DNA watermark encryption storage rack for strain protection, comprising: a storage rack body, a housing installed inside the storage rack body, multiple mounting slots symmetrically opened on the front of the housing, a DNA watermark encryption module installed on the top inner wall of the mounting slots, an alarm component installed on the top of the storage rack body, the alarm component including a monitoring alarm system, a controller, and an alarm, the monitoring alarm system being installed on the top of the storage rack body and connected to the DNA watermark encryption module, the controller being installed on the front of the monitoring alarm system, and the alarm being installed on the top of the monitoring alarm system.

[0008] Preferably, a through groove is provided on the inner wall of the back side of the mounting groove, and a movable groove is provided on the back side of the through groove.

[0009] Preferably, a storage drawer is slidably installed inside the mounting slot, and slide rails that are symmetrically installed on both sides of the storage drawer and slidably connected to the inner wall of the mounting slot are installed on the side of the storage drawer. A handle is installed on the front of the storage drawer.

[0010] Preferably, a support rod is installed on the back of the storage drawer, and the support rod extends into the interior of the movable groove through a through groove, with a limit plate installed at one end of the support rod.

[0011] Preferably, a marking plate is installed on the front of the housing, and the marking plate is located below the mounting groove.

[0012] Preferably, a base is installed at the bottom of the storage rack body, and a switch door is installed on the front of the storage rack body.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] 1. The DNA watermark encryption module located above the storage drawer in this invention can assign specific DNA watermark information to the microbial storage container and bind it to the storage location, achieving microbial identification and protection at the genetic level. During storage and retrieval, the operation can be controlled by verifying the DNA watermark information, effectively preventing unauthorized acquisition of microbial strains. If an anomaly is detected, the alarm system is immediately activated, promptly notifying staff to handle the situation and ensuring the security of microbial storage.

[0015] 2. In this utility model, when the storage drawer is in use, the back is supported by a support rod. The support rod moves inside the moving groove. Under the action of the support rod, the storage drawer can be fully pulled out without falling. After the storage drawer is fully pulled out, there is enough space to take out and store the bacteria, which makes it convenient to take out or store the bacteria.

[0016] 3. The structure of the cabinet and the labeling plate in this utility model facilitate the classification and management of microbial strains, improving the efficiency of storage management. Simultaneously, the overall structural design of the storage rack helps maintain a relatively stable internal environment, and combined with possible temperature and humidity control measures, provides more suitable long-term storage conditions for the microbial strains. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the box structure of this utility model;

[0019] Figure 3 This is a schematic diagram of the storage drawer structure of this utility model;

[0020] Figure 4 This is a schematic diagram of the monitoring and alarm system structure of this utility model.

[0021] In the diagram: 1. Storage rack body; 2. Base; 3. Opening / closing door; 4. Cabinet; 5. Mounting slot; 6. Marking plate; 7. Storage drawer; 8. Handle; 9. Slide rail; 10. DNA watermark encryption module; 11. Moving slot; 12. Through slot; 14. Support rod; 15. Limiting plate; 16. Monitoring and alarm system; 17. Controller; 18. Alarm. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0024] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0025] Please see Figure 1 , Figure 3 and Figure 4 DNA watermarked encryption storage rack for strain protection;

[0026] The system includes: a storage rack body 1 and an alarm component. A housing 4 is installed inside the storage rack body 1, and the housing 4 is tightly fitted to the inner wall of the storage rack body 1, with the opening of the housing 4 aligned with the front of the storage rack body 1. Multiple mounting slots 5 are symmetrically arranged on the front of the housing 4. A DNA watermark encryption module 10 is installed on the top inner wall of each mounting slot 5, with the bottom surface of the DNA watermark encryption module 10 flush with the top inner wall of the mounting slot 5, and its sensing area facing the inside of the mounting slot 5. An alarm component is installed on the top of the storage rack body 1, comprising a monitoring alarm system 16, a controller 17, and an alarm 18. The monitoring alarm system 16 is installed on the top of the storage rack body 1 and connected to the DNA watermark encryption module 10. The controller 17 is installed on the front of the monitoring alarm system 16, and the alarm 18 is installed on the top of the monitoring alarm system 16.

[0027] The storage rack body 1 provides an installation location for the internal cabinet 4, as well as for the alarm component. The interior of the cabinet 4 is used to store microbial cultures via storage drawers 7. The DNA watermark encryption module 10 located above the storage drawers 7 can mark or verify the placed microbial culture storage container with DNA watermarks, associating specific DNA watermark information with the storage location. It is also connected to the monitoring and alarm system 16. When abnormal DNA watermark information or illegal access is detected, the monitoring and alarm system 16 in the alarm component will issue an alarm signal, and finally, the alarm will be triggered by the alarm device 18. The controller 17 is used to control the use of the storage rack, and the monitoring and alarm system 16 will also issue a remote alarm signal to the staff so that the staff can arrive in time to handle the situation.

[0028] Please see Figure 1 , Figure 2 and Figure 3 DNA watermarked encryption storage rack for strain protection;

[0029] A through groove 12 is provided on the inner wall of the back of the mounting groove 5. A movable groove 11 is provided on the back of the through groove 12. A storage drawer 7 is slidably installed inside the mounting groove 5. Slide rails 9 that are slidably connected to the inner wall of the mounting groove 5 are symmetrically installed on both sides of the storage drawer 7. A handle 8 is installed on the front of the storage drawer 7. A support rod 14 is installed on the back of the storage drawer 7 and extends through the through groove 12 into the interior of the movable groove 11. A limit plate 15 is installed at one end of the support rod 14. A marking plate 6 is installed on the front of the box body 4 and is located below the mounting groove 5. A base 2 is installed at the bottom of the storage rack body 1. A door 3 is installed on the front of the storage rack body 1. The width of the through groove 12 is adapted to the diameter of the support rod 14 installed on the back of the storage drawer 7. The length direction of the movable groove 11 on the back of the through groove 12 is consistent with the pull-out direction of the storage drawer 7. The depth of the movable groove 11 is greater than the depth of the through groove 12 to provide moving space for the support rod 14 and limit its vertical displacement.

[0030] The interior of the storage drawer 7 is used to store microbial cultures. The storage drawer 7 is slidably installed via the slide rail 9. By pulling out the storage drawer 7, microbial cultures can be placed in or taken out of the storage drawer 7. When in use, the back of the storage drawer 7 is supported by the support rod 14. The support rod 14 moves inside the moving groove 11. Under the action of the support rod 14, the storage drawer 7 can be fully pulled out without falling. After the storage drawer 7 is fully pulled out, there is enough space for taking out and storing microbial cultures, which makes it easy to take out or store them. The limiting plate 15 is used to prevent the support rod 14 from being pulled out and plays a limiting role. The opening and closing door 3 is used to close the front of the storage rack body 1. The base 2 is used to support the bottom of the storage rack body 1.

[0031] In use, the storage rack body 1 is placed in the desired position via the base 2, the switch door 3 is opened, and the storage drawer 7 is pulled out, allowing staff to access the microbial cultures in the storage drawer 7. The storage rack is equipped with a DNA watermark encryption module 10 and an alarm system. When the microbial culture storage container is placed into the storage drawer 7 of the storage unit of the storage rack, the DNA watermark encryption module 10 assigns it specific DNA watermark information and binds it to the storage location. During access, the monitoring and alarm system is linked by verifying the DNA watermark information in the DNA watermark encryption module.

[0032] The role of the DNA watermark encryption module in the storage rack in this case:

[0033] Precise identification of bacterial strains: During the storage process, the DNA watermark encryption module assigns a unique DNA watermark to each bacterial storage container, essentially giving each strain a unique "genetic ID card." This allows for precise differentiation among numerous strains from different sources, types, and collection times, avoiding problems such as confusion, detachment, or tampering that may occur with traditional identification methods (such as labels or stickers), thus ensuring the accuracy and reliability of strain identification.

[0034] For example, a large microbial culture bank may contain microbial strains from multiple laboratories for various purposes. Through a DNA watermark encryption module, staff can quickly and accurately identify detailed information about each strain, providing a solid foundation for subsequent research, production, and management.

[0035] 2) Security and Anti-theft Tracking: This module, as the core component of the storage rack security system, effectively prevents the illegal acquisition and misuse of bacterial strains. Due to the high specificity and complexity of DNA watermark information, it is very difficult for criminals to forge or tamper with it, greatly improving the security of bacterial strain storage.

[0036] In the event of theft or suspected theft of bacterial strains, the source and destination of the strains can be traced by detecting and analyzing the DNA watermark information left at the scene. This provides strong evidence for law enforcement agencies, helps to quickly recover the stolen strains, and holds relevant parties accountable, thereby protecting the interests of research institutions, enterprises, and the entire biotechnology industry.

[0037] 3) Data Management and Quality Control: The detailed bacterial strain information recorded by the DNA watermark encryption module is linked to the location information of the storage rack, facilitating the storage management and data statistics of the strains. Researchers can quickly determine the specific location of a bacterial strain in the storage rack by querying the DNA watermark information, improving the efficiency of strain retrieval.

[0038] Meanwhile, regarding the quality control of bacterial strains, tracing the collection time and source information contained in the DNA watermark information allows for a better assessment of the strain's quality stability and reliability. If a problem is found with a particular strain during use, other strains from the same batch or source can be quickly located based on the DNA watermark information for comprehensive inspection and analysis, enabling timely measures to prevent the problem from escalating.

[0039] How the DNA watermark encryption module works:

[0040] 1) Watermark Information Assignment Process

[0041] Information Encoding and Synthesis: First, various information about the microbial strain is encoded according to pre-defined encoding rules. For example, the laboratory from which the strain originated may be assigned a binary code of a specific length, with each laboratory having a unique code combination. The strain type also has corresponding encoding methods, such as assigning different code segments according to classifications like bacteria, actinomycetes, yeasts, and molds. The collection time is accurate to the second and converted into a specific format of digital code. After combining these codes, specialized DNA synthesis equipment is used to synthesize DNA fragments with specific base sequences according to the encoded sequences. These DNA fragments are the core carriers of the watermark information.

[0042] Labeling and Adhesion: A suitable carrier material is selected, such as gold nanoparticles or magnetic microspheres. These carriers possess good dispersibility and biocompatibility, and can be surface-modified with active groups. The synthesized DNA fragment is chemically coupled to the carrier material under specific reaction conditions (e.g., in an environment containing appropriate buffer, with the temperature controlled between 20-30°C, pH between 7-8, and an appropriate amount of cross-linking agent added), allowing the DNA fragment to firmly bind to the carrier surface. Then, the carrier labeled with DNA watermark information is mixed with a special adhesive that can tightly adhere to the surface of the bacterial storage container. By coating the mixture onto the container surface and subjecting it to appropriate curing treatment (e.g., drying at a certain temperature), the DNA watermark information is stably attached to the container, completing the watermarking process.

[0043] 2) Verification process

[0044] Sample Collection and Pretreatment: When it is necessary to verify the DNA watermark information on the bacterial culture storage container, use a specialized sampling tool (such as a micro swab or fiber brush) to gently wipe the area with the DNA watermark without damaging the container or the bacterial culture, and collect a small amount of sample containing the DNA watermark information. Place the collected sample into a test tube containing a specific buffer solution, and gently shake or stir to dissociate the DNA molecules from the carrier and disperse them evenly in the buffer solution, preparing for subsequent detection.

[0045] Detection and Analysis: Detection is performed using a combination of nucleic acid amplification and gene sequencing technologies. First, PCR technology is used to amplify the DNA watermark sequence in the sample to improve detection sensitivity. Specific primers are designed to bind complementary to both ends of the DNA watermark sequence. Under the action of DNA polymerase, multiple cycles of denaturation, annealing, and extension processes result in a large-scale amplification of the target DNA sequence. Then, the amplified product is purified to remove impurities and excess primers. Next, a gene sequencer is used to sequence the purified DNA fragments to obtain the actual DNA base sequence. Finally, the sequenced sequence is compared with a standard DNA watermark sequence pre-stored in a database. The similarity between the two sequences is calculated using professional sequence analysis software. If the similarity reaches a preset threshold (e.g., above 95%), the verification is considered successful, indicating that the DNA watermark information in the bacterial storage container is correct and the bacterial strain is legitimate. If the similarity is below the threshold, the DNA watermark information is considered abnormal, potentially indicating illegal acquisition, tampering, or errors during storage. In this case, the monitoring and alarm system will activate the corresponding alarm procedure.

[0046] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A DNA watermark encryption storage rack for bacterial strain protection, characterized in that, Includes: a storage rack body (1), inside which a box (4) is installed, and multiple mounting slots (5) are symmetrically opened on the front of the box (4). A DNA watermark encryption module (10) is installed on the top inner wall of the mounting slot (5). An alarm component is installed on the top of the storage rack body (1). The alarm component includes a monitoring alarm system (16), a controller (17), and an alarm (18). The monitoring alarm system (16) is installed on the top of the storage rack body (1) and is connected to the DNA watermark encryption module (10). The controller (17) is installed on the front of the monitoring alarm system (16), and the alarm (18) is installed on the top of the monitoring alarm system (16).

2. The DNA watermark encryption storage rack for strain protection according to claim 1, characterized in that: A through groove (12) is provided on the inner wall of the back side of the mounting groove (5), and a moving groove (11) is provided on the back side of the through groove (12).

3. The DNA watermark encryption storage rack for strain protection according to claim 2, characterized in that: A storage drawer (7) is slidably installed inside the mounting groove (5). Slide rails (9) that are slidably connected to the inner wall of the mounting groove (5) are symmetrically installed on both sides of the storage drawer (7). A handle (8) is installed on the front of the storage drawer (7).

4. The DNA watermark encryption storage rack for strain protection according to claim 3, characterized in that: A support rod (14) is installed on the back of the storage drawer (7), and the support rod (14) extends into the interior of the moving slot (11) through the through slot (12). A limit plate (15) is installed at one end of the support rod (14).

5. The DNA watermark encryption storage rack for strain protection according to claim 1, characterized in that: A marking plate (6) is installed on the front of the housing (4), and the marking plate (6) is located below the mounting groove (5).

6. The DNA watermark encryption storage rack for strain protection according to claim 1, characterized in that: The storage rack body (1) has a base (2) installed at the bottom and a switch door (3) installed on the front.

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