CAR-T cell low-temperature storage device

Abstract

The utility model belongs to the technical field of cell storage, and particularly relates to a CAR-T cell low-temperature storage device which comprises a storage box, a box plate is fixedly connected to the front end face of the storage box, a plurality of through holes are formed in the front end face of the box plate, and containing structures are arranged in the through holes. The storage box comprises a plurality of storage structures, the storage structures are located in the storage box and fixedly connected with the inner rear wall of the storage box, diffusion structures are jointly arranged in the storage box and at the bottom of the storage box, the diffusion structures are located among the storage structures and do not make contact with the storage structures, and placing structures are slidably embedded in the storage structures. A plurality of storage structures are arranged in the storage box, and placement structures are arranged in the storage structures, so that when cells need to be stored or taken in the use process of the device, the single placement structure can be pulled out for taking and placing operation, cold air in the box can be prevented from rapidly escaping outwards, and the storage effect of the device is improved. The temperature in the box can be prevented from being greatly changed, and the storage of cells is facilitated.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the cell storage technical field, specifically CAR T cell low temperature storage device. BACKGROUND

[0002] Chimeric antigen receptor T cell (CAR-T cell) therapy is a cutting-edge cellular immunotherapy method, which can be improved by gene engineering technology T cell into a "super warrior" that can accurately identify and kill tumor cells. This therapy has shown great potential in the treatment of various malignant tumors, and has become a research hotspot in the field of tumor treatment. However, CAR-T cells face many challenges during in vitro culture and storage.

[0003] After searching, in the immunocyte low temperature storage device of announcement number CN214987445U, the utility model provides an immunocyte low temperature storage device, relates to the cell storage technical field, including: refrigeration device, storage device, refrigeration device and storage device are communicated through pipeline, storage device includes: storage box, vertical shaft, annular tray, the top surface in storage box is provided with first recess, the bottom surface in storage box is provided with second recess, one end of vertical shaft is rotatably connected in first recess, the other end of vertical shaft is rotatably connected in second recess, vertical shaft is fixedly connected with annular tray.

[0004] The above-mentioned cell storage device can be placed without being affected by the flask, and the flask containing immune cell solution can be taken and placed more conveniently, but there are still some problems in specific application:

[0005] 1、The above-mentioned device is opened by the box door when in use, and the cell is taken and placed, and the box door is directly opened, which can easily make the cold air in the box quickly dissipate, easily cause the temperature change in the box, and affect the low temperature storage of the cell;

[0006] 2、The above-mentioned device directly transmits the cold gas in the liquid nitrogen storage tank to the storage box through the connecting pipe, and the connecting pipe is located on the upper part of the storage box, so that the cold gas enters the box body, which can cause the temperature distribution in the box body to be inconsistent, and also affect the normal storage of the cell.

[0007] Therefore, a CAR-T cell low temperature storage device is proposed for the above-mentioned problems. Utility model content

[0008] (I) technical problem solved

[0009] The CAR-T cell low-temperature storage device solves the problems that the existing technology is easy to cause the quick dispersion of the cold air in the storage box and the uneven distribution of the cold air in the storage box when the liquid nitrogen storage tank sends the cold air to the inside of the storage box through the connecting pipe.

[0010] (II) Technical solutions

[0011] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a CAR-T cell low-temperature storage device, including the storage box, the front end surface of the storage box is fixedly connected with the box plate, a plurality of through holes are formed in the front end surface of the box plate, a plurality of the through holes are internally provided with receiving structures, and the plurality of receiving structures are located inside the storage box and are fixedly connected with the inner back wall of the storage box, the inside and the bottom of the storage box are jointly provided with diffusion structures, and the diffusion structures are located between the plurality of receiving structures and are not in contact, a plurality of the receiving structures are internally slidably embedded with placing structures, the bottom of the storage box is fixedly connected with supporting legs at four corners, the top of the storage box is fixedly connected with the connecting pipe, and the connecting pipe extends to the inside of the storage box and is fixedly connected with the top of the diffusion structure, and the end, away from the storage box, of the connecting pipe is fixedly connected with the liquid nitrogen storage tank.

[0012] Preferably, the receiving structure includes a receiving cylinder, and the receiving cylinder is located inside the through hole and is fixedly connected with the inner back wall of the storage box, guide sliding grooves are formed in the left side of the inner surface of the receiving cylinder and the right side of the inner surface, and a plurality of equidistantly distributed through holes are formed in the outer surface of the receiving cylinder.

[0013] Preferably, the placing structure includes a boat-shaped receiving main body, and the boat-shaped receiving main body is slidably connected with the receiving cylinder, a placing groove is formed in the top of the boat-shaped receiving main body, and slide rails are fixedly connected with the upper part of the left side wall and the upper part of the right side wall of the boat-shaped receiving main body, and the two slide rails are slidably connected with the two guide sliding grooves respectively.

[0014] Preferably, the front part of the boat-shaped receiving main body is flush with the front part of the box plate, a plurality of storage boxes are arranged in the inside of the placing groove, and a pull button is fixedly connected with the front end surface of the boat-shaped receiving main body.

[0015] Preferably, the diffusion structure includes a main hollow plate and a motor, a connecting hole is formed in the top of the main hollow plate, and the connecting hole is connected with the connecting pipe, a plurality of extension hollow plates are fixedly connected with the left side and the right side of the main hollow plate, and the plurality of extension hollow plates are located between the plurality of receiving structures respectively, and the plurality of extension hollow plates are in communication with the inside of the main hollow plate.

[0016] Preferably, the plurality of extended hollow plate top portions are provided with a plurality of first air outlet holes distributed at equal intervals, the main hollow plate bottom portion is fixedly connected with a bottom cover, the bottom cover bottom portion is fixedly connected with the inner lower wall of the storage box, the main hollow plate top portion is fixedly connected with the inner upper wall of the storage box, a plurality of second air outlet holes are formed in the top portion of the bottom cover, the motor output end is fixedly connected with a fixed rod, the main hollow plate is fixedly connected with the bottom portion of the storage box, the fixed rod penetrates through the bottom portion of the storage box and extends into the storage box, and three arc-shaped push plates are fixedly connected to the outer surface of the fixed rod.

[0017] (Three) beneficial effects

[0018] Compared with the prior art, the CAR-T cell low-temperature storage device has the following beneficial effects:

[0019] 1、In the utility model, a plurality of storage structures are arranged in the storage box, and each storage structure is provided with a placing structure, so that when the cells need to be stored or taken out, the single placing structure can be pulled out for taking and placing operation, and the taking and placing operation of the single placing structure can avoid opening the front part of the storage box directly, can avoid the rapid escape of the cold air in the box, can avoid the large change of the temperature in the box, and is more beneficial to the storage of the cells.

[0020] 2、In the utility model, the diffusion structure is arranged in the storage box, and when the device is used, the cold air in the liquid nitrogen storage tank enters the diffusion structure through the connecting pipe, is uniformly diffused into the box by the plurality of extended hollow plates, and since the cold air is relatively heavy, the cold air sinking to the bottom of the box is stirred by the arc-shaped push plate driven by the motor, so that the cold air can move and move in the box, the temperature in the box can be kept uniform, and the storage of the cells is more beneficial. DETAILED DESCRIPTION

[0021] The drawings described herein are used to provide further understanding of the utility model and constitute a part of the application. The schematic embodiments of the utility model and the description thereof are used to explain the utility model and do not constitute an improper limitation on the utility model. In the drawings:

[0022] Figure 1 is a three-dimensional structure schematic view of the utility model;

[0023] Figure 2 is a three-dimensional schematic view of the internal structure of the storage box of the utility model;

[0024] Figure 3 is a three-dimensional schematic view of the internal structure of the storage box of the utility model; Figure 2

[0025] Figure 4 ​It is the three-dimensional structure schematic view of the placing structure of the utility model;

[0026] Figure 5 It is the three-dimensional structure section view of the diffusion structure of the utility model.

[0027] In the figure: 1, storage box; 2, box board; 3, through hole; 4, storage structure; 5, diffusion structure; 6, placing structure; 7, support leg; 8, connecting pipe; 9, liquid nitrogen storage tank; 41, storage cylinder; 42, guide chute; 43, through hole; 61, boat-shaped storage main body; 62, placing groove; 63, slide rail; 64, storage box; 65, pull button; 51, main hollow plate; 52, connecting hole; 53, extended hollow plate; 54, first air outlet; 55, bottom cover; 56, second air outlet; 57, motor; 58, fixed rod; 59, arc-shaped push plate. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0029] The specific embodiments are given below.

[0030] Please refer to Figures 1-5 The utility model provides a kind of technical scheme: a CAR-T cell low-temperature storage device, including storage box 1, storage box 1 front end face is fixedly connected with box board 2, box board 2 front end face is equipped with multiple through holes 3, multiple through holes 3 are equipped with storage structure 4 inside, and multiple storage structures 4 are located inside storage box 1 and are fixedly connected with the rear wall in storage box 1, diffusion structure 5 is arranged in storage box 1 inside and bottom, and diffusion structure 5 is located between multiple storage structures 4 and does not contact, multiple storage structures 4 are slidably embedded with placing structure 6 inside, storage box 1 bottom four corners are fixedly connected with support leg 7, storage box 1 top is fixedly connected with connecting pipe 8, and connecting pipe 8 extends to storage box 1 inside and is fixedly connected with diffusion structure 5 top, liquid nitrogen storage tank 9 is fixedly connected to the end of connecting pipe 8 away from storage box 1.

[0031] Further, as Figures 1-4As shown, the storage structure 4 includes a storage cylinder 41, and the storage cylinder 41 is located inside the through hole 3 and is fixedly connected with the inner rear wall of the storage box 1. The left inner surface and the right inner surface of the storage cylinder 41 are both provided with a guide sliding groove 42. A plurality of through holes 43 are arranged on the outer surface of the storage cylinder 41 at equal distances. The placing structure 6 includes a boat-shaped storage main body 61, and the boat-shaped storage main body 61 is slidably connected with the storage cylinder 41. The boat-shaped storage main body 61 is provided with a placing groove 62 at the top. The upper left side wall and the upper right side wall of the boat-shaped storage main body 61 are both fixedly connected with a sliding rail 63, and the two sliding rails 63 are respectively slidably connected with the two guide sliding grooves 42. The front part of the boat-shaped storage main body 61 is flush with the front part of the box plate 2. A plurality of storage boxes 64 are arranged inside the placing groove 62. A pull button 65 is fixedly connected with the front end face of the boat-shaped storage main body 61.

[0032] Through the above scheme: by arranging a plurality of storage structures 4 in the storage box 1, and arranging a placing structure 6 in each of the plurality of storage structures 4, when the cells need to be stored or taken out during use of the device, a single placing structure 6 can be pulled out for taking and placing operation, which can avoid the rapid escape of cold air in the box, can avoid the large change of the temperature in the box, and is more beneficial to the storage of cells.

[0033] Further, as shown in Figure 1 , Figure 2 and Figure 5 , the diffusion structure 5 includes a main hollow plate 51 and a motor 57. The main hollow plate 51 is provided with a connecting hole 52 at the top, and the connecting hole 52 is connected with the connecting pipe 8. A plurality of extension hollow plates 53 are fixedly connected with the left side and the right side of the main hollow plate 51, and the plurality of extension hollow plates 53 are respectively located between the plurality of storage structures 4. The plurality of extension hollow plates 53 are all communicated with the inside of the main hollow plate 51. A plurality of first air outlets 54 are arranged on the top of the plurality of extension hollow plates 53 at equal distances. A bottom cover 55 is fixedly connected with the bottom of the main hollow plate 51. The bottom of the bottom cover 55 is fixedly connected with the lower inner wall of the storage box 1. The top of the main hollow plate 51 is fixedly connected with the upper inner wall of the storage box 1. A plurality of second air outlets 56 are arranged on the top of the bottom cover 55. A fixed rod 58 is fixedly connected with the output end of the motor 57. The main hollow plate 51 is fixedly connected with the bottom of the storage box 1. The fixed rod 58 penetrates through the bottom of the storage box 1 and extends into the inside of the storage box 1. Three arc-shaped push plates 59 are fixedly connected with the outer surface of the fixed rod 58 at the upper part. The three arc-shaped push plates 59 are all located inside the bottom cover 55.

[0034] Through the above scheme: by arranging the diffusion structure 5 inside the storage box 1, when the device is used, the cold air in the liquid nitrogen storage tank 9 enters the diffusion structure 5 through the connecting pipe 8, and is first diffused uniformly into the box by the plurality of extension hollow plates 53. Since the cold air is relatively heavy, the cold air sinking to the bottom of the box can be stirred by the arc-shaped push plates 59 driven by the motor 57, so that the cold air can move and move in the box, and the temperature in the box can be kept uniform, which is more beneficial to the storage of cells.

[0035] Working principle: the liquid nitrogen storage tank 9 is connected with the diffusion structure 5 in the storage box 1 through the connecting pipe 8 to provide the low-temperature environment required in the storage box 1, when the device is used, the labels can be pasted in classification in front of multiple placing structures 6, convenient for subsequent operation, when operating, the placing structure 6 is directly pulled out of the storage box 1, the cell can be taken and placed, the opening space of the storage box 1 can be reduced by pulling out the single placing structure 6, the temperature in the box can be kept stable by reducing the speed of cold air out of the box, the cold air in the liquid nitrogen storage tank 9 enters the diffusion structure 5 through the connecting pipe 8, first diffuses through the multiple extended hollow plates 53, so that the cold air can be distributed everywhere in the box, because the cold air is relatively heavy, the cold air will finally deposit at the bottom of the box, the motor 57 in the diffusion structure 5 can drive the multiple arc-shaped push plates 59 to rotate, so that the cold air deposited at the bottom of the box can float and walk, so that the temperature distribution in the box is uniform, which is beneficial to the storage of cells.

[0036] The basic principle, main features and advantages of the present application are shown and described above. It should be understood by those skilled in the art that the present application is not limited by the above embodiments, and the above embodiments and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the present application.

Claims

1. A CAR-T cell cryogenic storage device comprising a storage box (1), characterized in that: The front end face of the storage box (1) is fixedly connected with a box plate (2), a plurality of through holes (3) are formed in the front end face of the box plate (2), a plurality of storage structures (4) are arranged in the through holes (3), the storage structures (4) are arranged in the storage box (1) and are fixedly connected with the inner back wall of the storage box (1), a diffusion structure (5) is arranged in the storage box (1) and at the bottom, the diffusion structure (5) is arranged between the storage structures (4) and does not contact, a placing structure (6) is slidably arranged in the storage structure (4), support legs (7) are fixedly connected to the bottom of the storage box (1), a connecting pipe (8) is fixedly connected to the top of the storage box (1) and extends into the storage box (1) and is fixedly connected with the top of the diffusion structure (5), and a liquid nitrogen storage tank (9) is fixedly connected to the end of the connecting pipe (8) away from the storage box (1).

2. The CAR-T cell cryogenic storage device of claim 1, wherein: The storage structure (4) comprises a storage cylinder (41), and the storage cylinder (41) is arranged in the through hole (3) and is fixedly connected with the inner back wall of the storage box (1), guide sliding grooves (42) are formed in the left inner surface and the right inner surface of the storage cylinder (41), and a plurality of equidistantly distributed through holes (43) are formed in the outer surface of the storage cylinder (41).

3. The CAR-T cell cryogenic storage device of claim 1, wherein: The placing structure (6) comprises a boat-shaped storage main body (61), and the boat-shaped storage main body (61) is slidably connected with the storage cylinder (41), a placing groove (62) is formed in the top of the boat-shaped storage main body (61), and slide rails (63) are fixedly connected to the left side wall upper portion and the right side wall upper portion of the boat-shaped storage main body (61) and are slidably connected with the two guide sliding grooves (42), respectively.

4. The CAR-T cell cryogenic storage device of claim 3, wherein: The front part of the boat-shaped storage main body (61) is flush with the front part of the box plate (2), a plurality of storage boxes (64) are arranged in the placing groove (62), and a pull button (65) is fixedly connected to the front end face of the boat-shaped storage main body (61).

5. The CAR-T cell cryogenic storage device of claim 1, wherein: The diffusion structure (5) comprises a main hollow plate (51) and a motor (57), a connecting hole (52) is formed in the top of the main hollow plate (51) and is connected with the connecting pipe (8), a plurality of extension hollow plates (53) are fixedly connected to the left side and the right side of the main hollow plate (51) and are arranged between the storage structures (4), respectively, and the extension hollow plates (53) are in communication with the inside of the main hollow plate (51).

6. The CAR-T cell cryogenic storage device of claim 5, wherein: The top of each of the plurality of extended hollow plates (53) is provided with a plurality of first air outlets (54) distributed at equal distances, the bottom of the main hollow plate (51) is fixedly connected with a bottom cover (55), the bottom of the bottom cover (55) is fixedly connected with the lower wall in the storage box (1), the top of the main hollow plate (51) is fixedly connected with the upper wall in the storage box (1), the top of the bottom cover (55) is provided with a plurality of second air outlets (56), the output end of the motor (57) is fixedly connected with a fixed rod (58), the main hollow plate (51) is fixedly connected with the bottom of the storage box (1), the fixed rod (58) penetrates through the bottom of the storage box (1) and extends into the inside of the storage box (1), and the outer surface of the fixed rod (58) is fixedly connected with three arc-shaped push plates (59) at the upper part, and the three arc-shaped push plates (59) are all located in the inside of the bottom cover (55).

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