Micro-fluidic chip

Abstract

The utility model relates to a micro-fluidic chip which comprises a base body, a sample feeding channel, a sample discharging channel and a branch channel, the sample feeding channel, the sample discharging channel and the branch channel are all installed on the base body, the sample feeding channel is connected with the sample discharging channel through the branch channel, the chip further comprises a detection cavity, a spoiler, a micro-column and a flow control mechanism, the detection cavity, the spoiler and the micro-column are all mounted in the branch channel, and the flow control mechanism is mounted in the sample introduction channel and the branch channel. The flow control mechanism is used for controlling the flow of a sample, the sample sequentially passes through the micro-column and the spoiler, and the turbulence degree of fluid flowing is increased, so that the mixing process of the sample is accelerated, and the uniform mixing of the sample is realized.

Description

TECHNICAL FIELD

[0001] The utility model relates to micro -fluidic chip technical field especially is related to a micro -fluidic chip. BACKGROUND

[0002] In the field of biochemistry, drug screening, environmental monitoring and clinical diagnosis, microfluidic chip technology gradually becomes an important tool for research and application due to its advantages of high efficiency, micro amount, rapidness and integration. Microfluidic chip device realizes sample introduction, reaction, separation, detection and other functions in a small space through a microchannel system, greatly improving the analysis efficiency and accuracy. However, the traditional microfluidic chip still has many challenges in sample mixing, reaction control and detection sensitivity.

[0003] Microfluidic chip controls microfluids in micrometer scale. It integrates various modules required in biochemical reaction process on a small chip, so it is also called chip laboratory or micro total analysis system. The reaction occurs in a microchannel, with fast reaction speed, small reagent consumption, high throughput, low cost and high automation.

[0004] The utility model discloses a premixing micro -fluidic chip discloses a premixing micro -fluidic chip, its including upper chip body, be equipped with negative pressure interface and a plurality of sample injection holes on the upper chip body, the one end of upper chip body downward is equipped with mixing channel and reaction channel, the one end of mixing channel can communicate with sample injection hole, the other end of mixing channel and the one end of reaction channel meet, the other end of upper chip body can communicate with negative pressure interface, lower chip body, be equipped with with the reaction layer that reaction channel corresponds between lower chip body and upper chip body, this patent exists sample mixing uneven, and the mixing efficiency is slow.

[0005] Therefore, it is an urgent problem to provide a microfluidic chip that can uniformly mix samples. UTILITY MODEL CONTENT

[0006] The utility model discloses a micro -fluidic chip that overcomes the defects in the prior art.

[0007] The utility model discloses a micro -fluidic chip that overcomes the defects in the prior art.

[0008] According to one aspect of the utility model, a micro -fluidic chip is provided, including base body, sample injection channel, sample outlet channel, branch channel, the sample injection channel, sample outlet channel and branch channel all install on the base body, the sample injection channel passes through branch channel and sample outlet channel and is connected, the chip still includes detection chamber, spoiler, micro -column and flow control mechanism, detection chamber, spoiler and micro -column all install in branch channel, flow control mechanism installs in sample injection channel and branch channel.

[0009] As a preferred technical scheme, the flow control mechanism comprises an elastic valve film and a control electrode, the control electrode is connected with the elastic valve film, and the control electrode is installed below the elastic valve film.

[0010] As a preferred technical scheme, the flow control mechanism is installed at the outlet end and the inlet end of the branch channel.

[0011] As a preferred technical scheme, the flow control mechanism is installed at the connection between the sample inlet channel and the branch channel and away from the inlet of the sample inlet channel.

[0012] As a preferred technical scheme, the micro columns are spirally staggered.

[0013] As a preferred technical scheme, the spoiler is obliquely arranged on the branch channel.

[0014] As a preferred technical scheme, the detection cavity comprises a detection window and a detection element, the detection window is installed at the top of the detection cavity, and the detection element is installed in the detection window.

[0015] As a preferred technical scheme, the base body comprises a heating element and a temperature sensor, and both the heating element and the temperature sensor are installed at the bottom of the base body.

[0016] As a preferred technical scheme, the chip further comprises an electrical interface, the electrical interface is installed on the base body, and the electrical interface is connected with the flow control mechanism, the heating element and the temperature sensor respectively.

[0017] As a preferred technical scheme, the chip further comprises a reference channel, the reference channel is installed at the top of the base body (1) and communicates with the sample inlet channel.

[0018] Compared with the prior art, the chip has the following beneficial effects:

[0019] 1. The flow control mechanism controls the flow size of the sample, and the sample sequentially passes through the micro column and the spoiler, so that the turbulence degree of fluid flow is increased, the mixing process of the sample is accelerated, and uniform mixing of the sample is realized.

[0020] 2. The flow control mechanism comprises an elastic valve film and a control electrode, the electric field intensity is changed by changing the voltage or current on the control electrode, the deformation degree of the elastic valve film is controlled, the opening and closing state of the channel is controlled, the appropriate flow is provided, and the uniform mixing of the sample is realized.

[0021] 3. The micro column is spirally staggered, the spoiler is obliquely arranged on the branch channel, the turbulence degree of fluid flow is increased, the sample flow rate is increased, the mixing process is more intense, and the sample mixing is accelerated.

[0022] 4. The top surface of the base body is provided with a reference channel communicated with the sample inlet channel, for introducing reference solution or standard solution, which can be used for calibrating the detection element, eliminating background interference or improving detection accuracy. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0024] Figure 2 It is a schematic diagram of A-A section of the utility model;

[0025] Figure 3 It is a schematic diagram of B-B section of the utility model;

[0026] Figure 4 It is a bottom view of the utility model.

[0027] 1, base body; 2, sample inlet channel; 3, sample outlet channel; 4, branch channel; 5, detection cavity; 6, spoiler; 7, micro column; 8, elastic valve film; 9, electrical interface; 10, temperature sensor; 11, detection window; 12, reference channel; 13, heating element; A-A, first section; B-B, second section. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor should belong to the protection scope of the utility model.

[0029] In the sample mixing process of the traditional microfluidic chip, passive diffusion or laminar flow effect is often relied on, resulting in low mixing efficiency, especially in low flow rate or high viscosity samples. In addition, for experiments that require precise control of reaction conditions, the traditional chip lacks flexible and effective control mechanism, and it is difficult to realize precise regulation of reaction conditions. The utility model can realize rapid and efficient mixing of samples, precise control of reaction conditions and high sensitivity detection.

[0030] The utility model provides a kind of microfluidic chip;The utility model controls the flow size of sample by flow control mechanism, and sample is in turn through micro column, spoiler, and the turbulent degree of fluid flow is increased, to accelerate the mixing process of sample, and the uniform mixing of sample is realized.The flow control mechanism of the utility model includes elastic valve membrane and control electrode, and the electric field intensity is changed by changing voltage or current on control electrode, to control the deformation degree of elastic valve membrane, in turn control the opening and closing state of channel, provide suitable flow, realize the uniform mixing of sample.The micro column of the utility model is spirally staggered arrangement, and spoiler is obliquely arranged on branch channel, the turbulent degree of fluid flow is increased, sample flow rate is changed fast, mixing process is more intense, and sample mixing is accelerated.The top surface of the utility model substrate is provided with reference channel being communicated with sample inlet channel, for introducing reference solution or standard solution, these solutions can be used to calibrate detection element, eliminate background interference or improve detection accuracy.

[0031] Example 1

[0032] As Figures 1-4 shown, a kind of microfluidic chip, including substrate 1, sample inlet channel 2, sample outlet channel 3, branch channel 4, the sample inlet channel 2, sample outlet channel 3 and branch channel 4 are all installed on substrate 1, the sample inlet channel 2 is connected by branch channel 4 and sample outlet channel 3, and the chip further includes detection cavity 5, spoiler 6, micro column 7 and flow control mechanism, the detection cavity 5, spoiler 6 and micro column 7 are all installed in branch channel 4, and the flow control mechanism is installed in sample inlet channel 2 and branch channel 4.

[0033] The detection cavity 5 includes detection window 11 and detection element, the detection window 11 is installed in the top of detection cavity 5, and the detection element is installed in detection window 11.

[0034] In the embodiment, the substrate 1 is made of a material with good corrosion resistance, high temperature resistance and biocompatibility. In the embodiment, the substrate 1 is made of glass. The wake flow channel network is processed by using a precision machining technique to ensure that the channel size is accurate and the surface is smooth, thereby reducing fluid resistance and promoting uniform fluid flow. The substrate 1 is provided with two parallel sample inlet channels 2 and sample outlet channels 3. One end of each of the sample inlet channels 2 and the sample outlet channels 3 extends to one of the opposite side walls of the substrate 1, and is used to introduce a sample to be tested and discharge the treated sample, respectively. A plurality of detection cavities 5 are provided in the substrate 1 and between the sample inlet channels 2 and the sample outlet channels 3, and are used to accommodate the sample to be tested and allow the detection elements to analyze the sample. A plurality of branch channels 4 are provided in the substrate 1 and communicate with the detection cavities 5. The two ends of each of the branch channels 4 communicate with the sample inlet channels 2 and the sample outlet channels 3. A detection window 11 is provided at the top of each of the detection cavities 5, and a detection element is installed in the detection window 11. The detection window 11 is transparent and is usually made of glass or transparent polymer, allowing an external light source and a detection instrument to analyze the sample in the detection cavity 5 through the window. The detection element can be an optical sensor, an electrochemical sensor or a biosensor.

[0035] The flow control mechanism includes an elastic valve membrane 8 and a control electrode connected to the elastic valve membrane 8. The control electrode is installed below the elastic valve membrane 8. The flow control mechanism is installed at the outlet end and the inlet end of the branch channel 4. The flow control mechanism is installed at the connection between the sample inlet channel 2 and the branch channel 4 and away from the inlet of the sample inlet channel 2.

[0036] In the embodiment, the flow control mechanism includes elastic valve membranes 8 provided in the sample inlet channel 2 and the branch channel 4 and control electrodes for deforming the elastic valve membranes 8. The elastic valve membranes 8 are distributed at the inlet and outlet of the branch channel 4 and at the connection between the branch channel 4 and the sample inlet channel 2 and away from the inlet of the sample inlet channel 2 (i.e., perpendicular between adjacent elastic valve membranes 8). The control electrodes are installed below the elastic valve membranes 8. The elastic valve membranes 8 are made of soft and durable materials such as silicone and can deform in response to an electric field generated by the control electrodes. The control electrodes are usually thin films or coils made of conductive materials. The intensity of the electric field is changed by changing the voltage or current on the electrodes, thereby controlling the degree of deformation of the elastic valve membranes 8 and the opening and closing state of the channels.

[0037] The micro-pillars 7 are arranged in a helical staggered manner. The spoiler 6 is inclinedly arranged on the branch channel 4.

[0038] In the embodiment, the micro-column 7 is spirally staggered along the axial direction of the branch channel 4, the surface of the micro-column 7 is provided with a hydrophilic coating, and the spoiler 6 is arranged in the branch channel 4, the spoiler 6 is arranged on the side wall of the branch channel 4 on the side of the detection cavity 5, and the spoiler 6 is arranged at an angle, and the angle of the spoiler 6 is 30-60 degrees. The spiral staggered arrangement of the micro-column 7 and the oblique arrangement of the spoiler 6 can increase the turbulence degree of fluid flow, thereby accelerating the mixing process of the sample.

[0039] The base 1 comprises a heating element 13 and a temperature sensor 10, both of which are mounted on the bottom of the base 1.

[0040] The bottom surface of the base is provided with a heating element 13 and a temperature sensor 10, and the heating element 13 and the temperature sensor 10 are connected with an external temperature control circuit, which is used to control the temperature inside the base 1 to ensure that the experiment is carried out under constant temperature conditions. The temperature sensor 10 monitors the temperature inside the base 1 in real time and transmits data to the external temperature control circuit to accurately adjust the heating element 13.

[0041] The chip further comprises an electrical interface 9 mounted on the base 1, and the electrical interface 9 is connected with the flow control mechanism, the heating element 13 and the temperature sensor 10 respectively.

[0042] The base 1 is provided with an electrical interface 9 connected with external equipment, and the electrical interface 9 is used to connect the elastic valve membrane 8, the control electrode, the heating element 13 and the temperature sensor 10 with the external control and detection equipment. The heating element 13 is a thin film resistance heater, which covers the area corresponding to the microfluidic channel network on the lower surface of the chip base. The temperature sensor 10 is a thermistor, which is arranged on the lower surface of the chip base close to the detection cavity 5. The electrical interface 9 adopts a standardized connector and pin configuration to facilitate connection and communication with external equipment.

[0043] The chip further comprises a reference channel 12 mounted on the top of the base 1 and communicating with the sample inlet channel 2.

[0044] The top surface of the base 1 is provided with a reference channel 12 communicating with the sample inlet channel 2, which is used to introduce reference solution or standard solution. These solutions can be used to calibrate detection elements, eliminate background interference or improve detection accuracy.

[0045] In use, the sample to be tested is introduced into the sample inlet channel 2 by a syringe pump, the flow rate and speed of the syringe pump are adjusted to control the introduction speed and amount of the sample, the deformation degree of the elastic valve membrane 8 is controlled by changing the voltage or current on the electrode, thereby controlling the opening and closing of the channel, guiding the sample flow, when the sample flows in the branch channel 4, it encounters the micro columns 7 and the baffles 6 arranged in a spiral and staggered manner, increasing the turbulence degree of fluid flow, promoting the mixing of the sample, the heating element 13 is heated according to the temperature value set by the external temperature control circuit, the temperature sensor 10 monitors the temperature inside the substrate 1 in real time and transmits the data to the external temperature control circuit, the temperature control circuit accurately adjusts the heating element 13 according to the real-time monitored temperature value, to ensure that the experiment is carried out under constant temperature conditions, the mixed sample enters the detection cavity 5 and is analyzed by the detection element, the detection element converts the detected signal into an electric signal and transmits it to the external detection device through the electrical interface 9, the external detection device processes and analyzes the received signal to obtain the experimental results, and the processed sample is discharged from the substrate 1 through the sample outlet channel 3.

[0046] The above merely illustrates the specific implementation of the present application, but the protection scope of the present application is not limited to this, any skilled person in the art can easily think of various equivalent modifications or replacements within the technical range disclosed by the present application, and these modifications or replacements should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A microfluidic chip comprising a substrate (1), a sample inlet channel (2), a sample outlet channel (3), and a branch channel (4), the sample inlet channel (2), the sample outlet channel (3), and the branch channel (4) being mounted on the substrate (1), the sample inlet channel (2) being connected to the sample outlet channel (3) via the branch channel (4), characterized in that, The chip further comprises a detection cavity (5), a spoiler (6), a micro-column (7) and a flow control mechanism, the detection cavity (5), the spoiler (6) and the micro-column (7) are all installed in the branch channel (4), and the flow control mechanism is installed in the sample inlet channel (2) and the branch channel (4).

2. The microfluidic chip according to claim 1, wherein, The flow control mechanism comprises an elastic valve membrane (8) and a control electrode, the control electrode is connected with the elastic valve membrane (8), and the control electrode is installed below the elastic valve membrane (8).

3. The microfluidic chip of claim 1, wherein, The flow control mechanism is installed at the outlet end and the inlet end of the branch channel (4).

4. The microfluidic chip of claim 1, wherein, The flow control mechanism is installed at the joint of the sample inlet channel (2) and the branch channel (4) and away from the inlet of the sample inlet channel (2).

5. The microfluidic chip of claim 1, wherein, The micro-column (7) is spirally staggered.

6. The microfluidic chip of claim 1, wherein, The spoiler (6) is obliquely arranged on the branch channel (4).

7. The microfluidic chip of claim 1, wherein, The detection cavity (5) comprises a detection window (11) and a detection element, the detection window (11) is installed at the top of the detection cavity (5), and the detection element is installed in the detection window (11).

8. The microfluidic chip of claim 1, wherein, The base body (1) comprises a heating element (13) and a temperature sensor (10), and the heating element (13) and the temperature sensor (10) are both installed at the bottom of the base body (1).

9. The microfluidic chip of claim 8, wherein, The chip further comprises an electrical interface (9), the electrical interface (9) is installed on the base body (1), and the electrical interface (9) is connected with the flow control mechanism, the heating element (13) and the temperature sensor (10) respectively.

10. The microfluidic chip of claim 1, wherein, The chip further comprises a reference channel (12), the reference channel (12) is installed at the top of the base body (1) and communicates with the sample inlet channel (2).

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