Sampling system for litsea cubeba rhizosphere microorganism monitoring
By designing a sampling vehicle with wheels and a dry ice-cooled storage box, the problems of inconvenient portability and high power consumption of soil microbial sampling tools were solved, enabling convenient low-temperature preservation and low-energy operation of samples.
Patent Information
- Application Number
- CN202520302939.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-25
- Publication Date
- 2026-02-27
- Estimated Expiration
- 2035-02-25
AI Technical Summary
In existing technologies, soil microbial sampling tools and sample storage are inconvenient to carry, and the need for electric cooling results in large equipment size and high energy consumption. The state of the sample is also easily changed between sampling and sequencing.
A sampling system comprising a sampling vehicle, a storage box, and a preservation box was designed. The sampling vehicle is equipped with wheels, the storage box contains a tool chamber, and the preservation box contains a cooling chamber and a sample chamber. Dry ice refrigerant is used for low-temperature preservation, avoiding the use of electricity.
It enables convenient sample transport and cryogenic preservation, reduces energy consumption, simplifies the structure, and reduces the risk of sample state changes.
Smart Images
Figure CN223949615U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to soil microorganism monitoring technical field, especially relate to a sampling system for monitoring rhizosphere microorganism of litsea cubeba. BACKGROUND
[0002] Soil microorganism is decomposer in ecological system, they make organic matter decompose, release nutrient, supply plant utilization, have very important influence to plant. The quantity, distribution and activity situation of microorganism in soil reflect the height of soil fertility, and monitoring soil microorganism is beneficial to people to better understand the interaction of soil-microorganism-plant-environment. Monitoring the microorganism in the soil of plant root needs to sample rhizosphere soil, and needs to use hoe, brush and other auxiliary tools when sampling, and the obtained sample is stored by using storage container, and the sample needs to be sequenced and detected after sampling, and the basic soil nutrient indexes such as moisture content, carbon, nitrogen and phosphorus, pH, etc. are detected. Since the sampling site is often difficult for vehicles to enter, the sampling personnel need to enter on foot, which leads to the inconvenience of carrying and transferring tools and sample storage containers. In addition, since sequencing needs to be performed in a professional sequencing institution, on-site sequencing cannot be performed, which causes a certain time difference between sampling and sequencing operations, and the state of microorganisms in the sample can easily change. Therefore, the storage container often needs to have a low-temperature storage function, and electric refrigeration not only leads to a large equipment size, but also is inconvenient to carry and consumes a lot of energy. SUMMARY
[0003] The utility model aims at solving one of the technical problems in the prior art. To this end, the utility model provides a sampling system for monitoring rhizosphere microorganism of litsea cubeba, which is convenient to carry and transfer, and can store samples at low temperature without using electricity.
[0004] The sampling system for monitoring rhizosphere microorganism of litsea cubeba according to the utility model embodiment comprises:
[0005] A sampling vehicle is provided with movable wheels at the bottom.
[0006] A storage box is arranged on the sampling vehicle, and the storage box is provided with a storage drawer that can be horizontally pulled out, and the storage drawer is defined with a tool cavity for storing sampling tools.
[0007] A storage box is arranged on the sampling vehicle, and the storage box comprises a box body and a box cover arranged on the top of the box body, the box body is provided with a cooling cavity and a first sample cavity at intervals, a refrigerant is arranged in the cooling cavity, and a plurality of first sample bottles are arranged in the first sample cavity.
[0008] The sampling system for monitoring rhizosphere microorganism of litsea cubeba according to the utility model embodiment has at least the following beneficial effects:
[0009] In use, the sampling vehicle can be moved to a sampling site, then the storage drawer is opened to take out the sampling tool, a certain number of first sample bottles are taken out from the first sample cavity, then the sampling tool is used to sample the soil at the root of the Litsea cubeba, the first sample bottles are used to contain the samples, after sampling, the first sample bottles can be put back into the first sample cavity and the box cover is closed, the refrigerant is used for refrigeration to store the samples at low temperature, so as to ensure the state of the microorganisms in the samples. The sampling system for monitoring rhizosphere microorganisms of Litsea cubeba has the advantages of convenient carrying and transferring, low energy consumption, simple overall structure, small occupied space and the like, and the samples are stored at low temperature by using the refrigerant to refrigerate in the storage box, without using electric energy.
[0010] According to some embodiments of the present application, a partition is horizontally arranged in the box body, the first sample cavity is defined on the upper side of the partition, and the cooling cavity is defined on the lower side of the partition.
[0011] According to some embodiments of the present application, the refrigerant is dry ice.
[0012] According to some embodiments of the present application, the cooling cavity is open on one side in the horizontal direction, and a detachable dry ice drawer is arranged in the cooling cavity, the dry ice drawer closes the open end of the cooling cavity, and the dry ice drawer is used for placing the dry ice.
[0013] According to some embodiments of the present application, a first sample holder is detachably arranged in the first sample cavity, a plurality of vertically extending first placing grooves are arranged on the first sample holder, and the first sample bottles are inserted into the first placing grooves.
[0014] According to some embodiments of the present application, there is a gap between the side wall of the first sample cavity and the first sample holder in the horizontal direction.
[0015] According to some embodiments of the present application, the first sample holder is provided with a vertically extending positioning hole, a vertically extending positioning column is arranged on the upper surface of the partition, the positioning column is inserted into the positioning hole, and the first sample holder is horizontally positioned.
[0016] According to some embodiments of the present application, a limiting part is rotatably arranged in the first sample cavity, the limiting part has a locking state and an unlocking state during rotation, when the limiting part is in the locking state, the limiting part is located on the upper side of the first sample holder, so as to limit the upward displacement of the first sample holder and keep the state that the positioning column is inserted into the positioning hole, and when the limiting part is in the unlocking state, the limiting part avoids the vertical movement path of the first sample holder.
[0017] According to some embodiments of the utility model, the partition board upper surface is provided with vertical extension mounting column, mounting column is located the first sample frame's circumference side, mounting column is provided with outer thread, the limiting portion is provided with threaded hole, and is installed on the mounting column in screw thread.
[0018] According to some embodiments of the utility model, the storage drawer further defines a second sample cavity, and a plurality of second sample bottles are placed in the second sample cavity.
[0019] Additional aspects and advantages of the utility model will be partially given in the following description, some will become obvious from the following description, or be understood by the practice of the utility model. BRIEF DESCRIPTION OF DRAWINGS
[0020] The utility model will be further explained in combination with the drawings and examples, wherein:
[0021] Figure 1 It is the structure schematic drawing of sampling system for monitoring the rhizosphere microorganism of litsea cubeba of the utility model embodiment;
[0022] Figure 2 It is the explosion drawing of the storage box of the utility model embodiment;
[0023] Figure 3 It is the explosion drawing of the storage box of the utility model embodiment;
[0024] Figure 4 It is the structure schematic drawing of the first sample frame of the utility model embodiment.
[0025] Reference numerals:
[0026] Sampling vehicle 100, mobile wheel 110;
[0027] Storage box 200, storage drawer 210, tool cavity 211, second sample cavity 212, second sample frame 220, second placement groove 221;
[0028] Storage box 300, box 310, cooling cavity 311, first sample cavity 312, partition board 313, air hole 314, dry ice drawer 315, positioning column 316, limiting portion 317, mounting column 318, box cover 320, first sample frame 330, first placement groove 331, positioning hole 332;
[0029] First sample bottle 400;
[0030] Second sample bottle 500. DETAILED DESCRIPTION
[0031] The embodiments of the present application are described below in detail, examples of which are shown in the drawings, wherein the same or similar notations represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by reference to the drawings are exemplary only, and are used only for explaining the present application, and cannot be understood as a limitation of the present application.
[0032] In the description of the present application, it should be understood that, in relation to the orientation description, for example, the orientation or position relationship indicated by up, down and the like is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element indicated must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.
[0033] In the description of the present application, more than two means more than two. If there is a description of first, second, it is only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the sequence of indicated technical features.
[0034] In the description of the present application, unless otherwise explicitly limited, the words such as setting, installing, connecting and the like should be broadly understood, and the person skilled in the art can reasonably determine the specific meaning of the above words in the present application in combination with the specific content of the technical scheme.
[0035] Referring to Figures 1 to 4 The sampling system for monitoring rhizosphere microorganisms of Chinese Litsea Root of an embodiment of the present application comprises: a sampling vehicle 100, a storage box 200, and a storage box 300.
[0036] The bottom of the sampling vehicle 100 is provided with moving wheels 110 to facilitate movement.
[0037] The storage box 200 is arranged on the sampling vehicle 100, and the storage box 200 is provided with a horizontally drawable storage drawer 210, and the storage drawer 210 is limited to have a tool cavity 211 for storing sampling tools (not shown in the figure). It can be conceived that the sampling tools can include a hoe and a brush, the hoe is used for digging soil to make the root of the Chinese Litsea to leak out, and the brush is used for sweeping the soil on the root surface. It can be conceived that while sampling the rhizosphere soil, sampling of the soil outside the root is often also needed, so the sampling tools can also include a shovel, which can be used for shoveling the soil outside the root.
[0038] The storage box 300 is arranged on the sampling vehicle 100, the storage box 300 comprises a box body 310 and a box cover 320 arranged on the top of the box body 310, the box body 310 is internally and spacedly provided with a cooling cavity 311 and a first sample cavity 312, the cooling cavity 311 is internally provided with a refrigerant, and a plurality of first sample bottles 400 are arranged in the first sample cavity 312, the first sample bottles 400 are used for storing samples, and a plurality of first sample bottles 400 can be arranged.
[0039] In use, the sampling vehicle 100 can be moved to a sampling site, then the storage drawer 210 is opened to take out sampling tools, a certain number of first sample bottles 400 are taken out from the first sample cavity 312, then the sampling tools are used to sample the soil at the root of the Litsea cubeba, the first sample bottles 400 are used to contain the samples, after sampling, the first sample bottles 400 can be placed back into the first sample cavity 312 and the box cover 320 is closed, the refrigerant is used to cool to store the samples at low temperature, and the state of microorganisms in the samples is ensured.
[0040] It is conceived that, in order to prevent the storage drawer 210 and the box cover 320 from being moved at will, a locking structure can be arranged between the storage drawer 210 and the storage box 200 and between the box cover 320 and the box body 310.
[0041] Referring to FIGS. 1-3, Figure 1 and Figure 2 In some embodiments of the present application, a partition plate 313 is horizontally arranged in the box body 310, the first sample cavity 312 is defined on the upper side of the partition plate 313, and the cooling cavity 311 is defined on the lower side of the partition plate 313, that is, the partition plate 313 divides the internal space of the box body 310 into the first sample cavity 312 and the cooling cavity 311 which are arranged in an upper-lower opposite manner, and a plurality of air holes 314 are arranged on the partition plate 313.
[0042] It is conceived that the refrigerant can be in a liquid or solid state, and liquid nitrogen or dry ice can be used, in the present embodiment, the refrigerant is in the form of dry ice (not shown in the figure), and the refrigeration effect is good and the storage is convenient.
[0043] Further, the cooling cavity 311 is open on one side in the horizontal direction, and a detachable dry ice drawer 315 is arranged in the cooling cavity 311, the dry ice drawer 315 can be connected with the box body 310 through fasteners, the dry ice drawer 315 closes the open end of the cooling cavity 311 and can be taken out from the open end of the cooling cavity 311, and the dry ice drawer 315 is used for placing dry ice; by arranging the dry ice drawer 315 for placing dry ice, the dry ice can be conveniently added and replaced. It should be noted that, in order to prevent explosion, some small holes communicating with the outside can be arranged on the side wall of the box body 310 or the dry ice drawer 315, so as to avoid that the cooling cavity 311 is completely closed.
[0044] With reference to Figure 1 , Figure 2 and Figure 4 , in some embodiments of the present application, a first sample holder 330 is detachably arranged in the first sample cavity 312, a plurality of first placing grooves 331 extending in the vertical direction are arranged on the first sample holder 330, and the first sample bottle 400 is inserted into the first placing groove 331 to be horizontally positioned by the first placing groove 331; in this embodiment, the first sample bottle 400 is placed by arranging the detachable first sample holder 330 and arranging the first placing groove 331 on the first sample holder 330, on the one hand, the first sample bottle 400 can be prevented from shaking when the sampling vehicle 100 is moved, and on the other hand, the first sample bottle 400 can be concentrated by the first sample holder 330, when the samples include multiple portions, the first sample holder 330 can be taken out to take out all the first sample bottles 400 containing samples, and then the first sample bottles 400 are transferred to the detection instrument for detection, so that the operation is convenient.
[0045] It is conceivable that, in order to improve the cooling effect of the first sample bottle 400, a hollow structure can be arranged on the side wall of the first placing groove 331 on the first sample holder 330, so that the cold air can contact the outer wall of the first sample bottle 400.
[0046] It is conceivable that, in order to facilitate taking the first sample holder 330, a handle can be arranged on the first sample holder 330.
[0047] With reference to Figure 1 , in some embodiments of the present application, a gap is arranged between the side wall of the first sample cavity 312 and the first sample holder 330 in the horizontal direction, by arranging the gap, the cold air can enter the upper side of the first sample holder 330, and the cold air can fully contact the first sample bottle 400 on the first sample holder 330.
[0048] With reference to Figure 1 , Figure 2 and Figure 4As shown, in some embodiments of the utility model, first sample holder 330 is provided with vertically extending positioning hole 332, and the upper surface of partition plate 313 is provided with vertically extending positioning column 316, positioning column 316 is inserted into positioning hole 332 to horizontally position first sample holder 330. It can be understood that, since there is a gap between the sidewall of first sample cavity 312 and first sample holder 330 in the horizontal direction, in order to prevent first sample holder 330 from horizontally shaking, the above structure is provided to horizontally position first sample holder 330.
[0049] Further, a limiting part 317 is rotatably arranged in first sample cavity 312, limiting part 317 has a locked state and an unlocked state during rotation, when limiting part 317 is in the locked state (for example, as shown in the figure), limiting part 317 is located on the upper side of first sample holder 330 to limit the upward displacement of first sample holder 330 and keep the state that positioning column 316 is inserted into positioning hole 332, when limiting part 317 is in the unlocked state, limiting part 317 avoids the vertical movement path of first sample holder 330, when it is needed to take out first sample holder 330, limiting part 317 can be rotated to the unlocked state to make limiting part 317 avoid the vertical movement path of first sample holder 330, then first sample holder 330 can be taken out upward, which is simple in structure and convenient to operate. Figure 1
[0050] Based on the above setting, in some specific embodiments, the upper surface of partition plate 313 is provided with vertically extending mounting column 318, mounting column 318 is located on the side of first sample holder 330 and passes through the gap between the sidewall of first sample cavity 312 and first sample holder 330, mounting column 318 is provided with external threads, limiting part 317 is provided with threaded hole, limiting part 317 is screw-mounted on mounting column 318 through threaded hole, by screwing limiting part 317 on mounting column 318, on the one hand, it can be convenient to rotate limiting part 317, on the other hand, it can be convenient to fix the position of limiting part 317 after rotation, which is simple in structure and strong in practicality.
[0051] Referring to Figure 1 and Figure 3 As shown, in some embodiments of the present application, a second sample cavity 212 is further defined in the storage drawer 210, and a plurality of second sample bottles 500 are placed in the second sample cavity 212. Specifically, a second sample rack 220 is detachably arranged in the second sample cavity 212, and a plurality of second placing grooves 221 are arranged on the second sample rack 220 for placing the second sample bottles 500. It can be understood that when sampling the soil at the root part of the Litsea cubeba, in addition to the need for detecting the microorganisms in the rhizosphere soil, the water content, carbon, nitrogen, phosphorus, pH and other basic soil nutrient indicators also need to be detected, so for the same Litsea cubeba tree, at least two portions of rhizosphere soil are needed, one is used for sequencing the microorganisms, and the other is used for detecting the water content, carbon, nitrogen, phosphorus, pH and other basic soil nutrient indicators, the former needs to be stored at low temperature, and the latter can be stored at normal temperature, in order to avoid that the volume of the storage box 300 is too large and the refrigeration capacity is increased, therefore, in the present embodiment, the second sample cavity 212 is arranged in the storage drawer 210 to store part of the samples at normal temperature.
[0052] When sampling, the soil is dug along the outer edge of the tree crown to the direction of the tree trunk until the root system is exposed, the fine roots are dug, the excess soil attached to the periphery of the roots is shaken off, and part of the soil on the surface of the roots is swept into the first sample bottle 400 by using a brush, and part of the soil is swept into the second sample bottle 500, the sample in the first sample bottle 400 is used for sequencing, and the sample in the second sample bottle 500 is used for detecting the water content, carbon, nitrogen, phosphorus, pH and other basic soil nutrient indicators.
[0053] It can be conceived that the second sample rack 220 can adopt the same structure and the same mounting mode as the first sample rack 330, and therefore the structure of the second sample rack 220 will not be specifically described in the present embodiment.
[0054] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms is not necessarily for the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in the present application.
[0055] The embodiments of the present application are described in detail above in combination with the drawings, but the present application is not limited to the above embodiments, and various changes can be made within the knowledge range of those skilled in the art without departing from the purpose of the present application.
Claims
1. A sampling system for monitoring rhizosphere microbes of Litsea cubeba, characterized in that, The utility model relates to a sampling trolley, which comprises a sampling trolley provided with moving wheels at the bottom, a storage box provided on the sampling trolley, the storage box being provided with a horizontally-drawable storage drawer, the storage drawer being internally defined with a tool cavity for storing sampling tools, a preservation box provided on the sampling trolley, the preservation box comprising a box body and a box cover provided on the top of the box body, the box body being internally spaced apart with a cooling cavity and a first sample cavity, the cooling cavity being internally provided with a refrigerant, and the first sample cavity being internally placed with a plurality of first sample bottles, the box body being internally horizontally provided with a partition plate, the upper side of the partition plate being defined with the first sample cavity, the lower side of the partition plate being defined with the cooling cavity, and the partition plate being provided on the upper side with a plurality of air holes, the refrigerant being dry ice, the cooling cavity being open along one side in the horizontal direction, the cooling cavity being internally provided with a detachable dry ice drawer, the dry ice drawer closing the open end of the cooling cavity, and the dry ice drawer being used for placing the dry ice, the first sample cavity being internally provided with a detachable first sample holder, the first sample holder being provided on the upper side with a plurality of vertically-extending first placing grooves, and the first sample bottles being inserted into the first placing grooves, the side wall of the first sample cavity and the first sample holder having a gap in the horizontal direction, the first sample holder being provided with a vertically-extending positioning hole, the upper surface of the partition plate being provided with a vertically-extending positioning column, the positioning column being inserted into the positioning hole to horizontally position the first sample holder, the first sample cavity being rotationally provided with a limiting part, the limiting part having a locked state and an unlocked state during rotation, the limiting part being located on the upper side of the first sample holder when in the locked state to limit the upward displacement of the first sample holder and keep the positioning column inserted into the positioning hole, and the limiting part avoiding the vertical movement path of the first sample holder when in the unlocked state, the upper surface of the partition plate being provided with a vertically-extending mounting column, the mounting column being located on the lateral side of the first sample holder, the mounting column being provided on the upper side with external threads, the limiting part being provided on the upper side with a threaded hole and being threadedly mounted on the mounting column, and the storage drawer being internally further defined with a second sample cavity, the second sample cavity being internally placed with a plurality of second sample bottles. 2. The sampling system for monitoring the rhizosphere microbes of Litsea cubeba according to claim 1, characterized in that: 3. The sampling system for monitoring the rhizosphere microbes of Litsea cubeba according to claim 2, characterized in that: 4. The sampling system for monitoring the rhizosphere microbes of Litsea cubeba according to claim 1, characterized in that: