Inoculating needle for quantitative collection of bacterial colonies

Abstract

The utility model relates to the technical field of microbial culture, in particular to an inoculating needle for quantitatively collecting bacterial colonies, which comprises a sampling needle and a scraping tube, a groove is arranged inside one end of the sampling needle, an opening is arranged at one end of the sampling needle close to the groove and communicated with the groove, a connecting port is arranged on the side wall of one end of the sampling needle close to the groove, and the scraping tube is communicated with the opening. The connecting port is communicated with the groove, the inner wall of the connecting port, the inner wall of the groove and the end part of one end, close to the groove, of the sampling needle are all provided with anti-skid holes, the outer side wall of the sampling needle is sleeved with the scraping pipe, and the inner wall of the scraping pipe is in sliding connection with the outer side wall of the sampling needle, so that automation is easier to realize in the operation process; and the working efficiency and accuracy of a laboratory can be improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of microorganism culture, specifically, relates to a inoculation needle for colony quantitative collection. BACKGROUND

[0002] In the microorganism experiment, the inoculation needle is the essential tool of picking the mycelium block, and is usually made by attaching a section of nickel-chromium alloy inoculation hook to the front end of a lead rod. But the inoculation ring is difficult to completely remove the microorganism after being used for many times, even if after the flame burning, some spores and other strong tolerance microorganism structures may still remain, leading to the occurrence of cross contamination. Subsequently, the disposable plastic inoculation ring emerges as the times require, and these materials have good chemical stability, biocompatibility and processing performance, which can meet the requirements of disposable use, and are also convenient for large-scale production and cost reduction. However, these disposable inoculation rings cannot quantitatively sample the bacteria in the process of preparing the bacterial liquid, which increases a great workload when adjusting the subsequent bacterial liquid concentration, and it is difficult to meet the requirements when the bacteria are fastidious, the bacterial colonies are viscous, and the bacterial colonies are granular and dry, and it is difficult to realize automation. SUMMARY

[0003] In view of the above, the utility model provides an inoculation needle for colony quantitative collection, which aims to solve at least one of the problems in the above background technology.

[0004] The utility model provides a kind of inoculation needle for colony quantitative collection, comprising: sampling needle, recess is arranged in one end of the sampling needle, and the one end of the sampling needle close to the recess is provided with opening, the opening is communicated with the recess, the side wall of the one end of the sampling needle close to the recess is provided with connecting port, the connecting port is communicated with the recess, the inner wall of the connecting port and the inner wall of the recess and the end of the one end of the sampling needle close to the recess are all provided with anti-skid hole, and fiber layer is provided on the surface of the anti-skid hole.

[0005] Scraper tube, the scraper tube is sleeved on the outer side wall of the sampling needle, and the inner wall of the scraper tube is slidably connected with the outer side wall of the sampling needle.

[0006] In some embodiments, the transverse section of the sampling needle is a circular structure.

[0007] In some embodiments, the outer diameter of the middle part of the recess is greater than the outer diameter of the two ends of the recess.

[0008] In some embodiments, the recess coincides with the axis of the sampling needle.

[0009] In some embodiments, the opening coincides with the axis of the recess, and the transverse section of the opening is a circular structure.

[0010] In some embodiments, the transverse section of the connecting port is a sector structure.

[0011] In some embodiments, the connecting ports are arranged in a ring array with the opening as the axis.

[0012] In some embodiments, each of the connecting ports is in communication with the opening.

[0013] In some embodiments, the inner diameter of the opening is smaller than the outer diameter of the middle part of the groove.

[0014] In some embodiments, the inner diameter of the scraping tube matches the outer diameter of the sampling needle.

[0015] Compared with the prior art, the beneficial effects of the present application are that the wrapped fiber layer further enhances the adsorption capacity of the bacterial colony, especially for those bacterial colonies that are easy to slide off, such as wet bacterial colonies or bacterial colonies growing on some less viscous culture media. The fiber can firmly fix the bacterial colony on the inoculation needle to prevent the bacterial colony from falling off the inoculation needle. The matching scraping tube is a key component for realizing quantitative collection. After the sampling needle collects the bacterial colony, the scraping tube can scrape off the excess bacterial colony outside the sampling needle. In this way, the amount of bacterial colony left on the sampling needle can be accurately controlled, only the bacterial colony absorbed by the 3-prong structure is retained, and the excess part is removed, thereby realizing accurate control of the amount of collected bacterial colony. Since quantitative collection can be realized, the experimenter can accurately adjust the bacterial liquid concentration according to the experimental requirements. Through accurate control of the amount of collected bacterial colony, bacterial liquids with different concentration gradients can be easily prepared, which is of great significance for studying the growth characteristics of microorganisms and the influence of drugs on microorganisms. The design of the new inoculation needle makes it easier to realize automation during operation, which helps to improve the efficiency and accuracy of laboratory work.

[0016] The foregoing general description and the following detailed description are merely exemplary and explanatory, rather than limiting the present disclosure.

[0017] Other features and aspects of the present disclosure will become more apparent from the following detailed description of exemplary embodiments with reference to the attached drawings. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the drawings required in the specific embodiments or the prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are some embodiments of the present application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0019] Fig. 1The axial drawing of the inoculation needle for quantitative collection of bacterial colonies is provided for the embodiments of the utility model.

[0020] Fig. 2 The front view of the inoculation needle for quantitative collection of bacterial colonies is provided for the embodiments of the utility model.

[0021] Fig. 3 The axial drawing of the inoculation needle for quantitative collection of bacterial colonies is provided for the embodiments of the utility model.

[0022] 1, sampling needle; 2, groove; 3, opening; 4, connecting port; 5, anti-skid hole; 6, scraping tube. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the application will be clearly and completely described in connection with the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the application.

[0024] In the description of the application, it should be understood that the terms "center", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only used to facilitate the description of the application and simplify the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the application.

[0025] The terms "first", "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more features. In the description of the application, unless otherwise specified, the meaning of "multiple" is two or more.

[0026] In the description of the application, it should be noted that, unless otherwise specified and limited, the terms "mounting", "connection", "connection" should be understood broadly, for example, it can be fixed connection, or detachable connection, or integral connection; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, or the communication between two elements. For those skilled in the art, the specific meaning of the above terms in the application can be understood according to the specific circumstances.

[0027] As described in the background section, in microbial experiments, inoculation needles are essential tools for picking up mycelial blocks, typically made by attaching a nickel-chromium alloy inoculation hook to the tip of a lead rod. However, after repeated use, these inoculation loops come into contact with microorganisms that are difficult to completely remove. Even after flame burning, some spores and other resistant microbial structures may still remain, leading to cross-contamination. Subsequently, disposable plastic inoculation loops emerged. These materials possess good chemical stability, biocompatibility, and processing performance, meeting the requirements for single use while also facilitating large-scale production and cost reduction. However, these disposable inoculation loops cannot quantitatively sample bacteria during bacterial suspension preparation, significantly increasing the workload for subsequent bacterial suspension concentration adjustments. Furthermore, they are difficult to use for fastidious bacteria, viscous colonies, and granular dry colonies, hindering automation.

[0028] To address the aforementioned issues, this application proposes an inoculation needle for quantitative colony collection. The fiber layer further enhances the adsorption capacity for colonies, especially for easily slippery colonies, such as moist colonies or those growing on less viscous culture media. The fibers firmly fix the colonies to the inoculation needle, preventing them from detaching. The accompanying scraper is a key component for quantitative collection. After collecting colonies with the sampling needle, the scraper removes excess colonies from the outside of the needle. This method allows for precise control of the amount of colonies remaining on the sampling needle, retaining only those adsorbed by the three-pronged porous structure while discarding the excess, thus achieving accurate control over the collected colony quantity. Because quantitative collection is possible, researchers can accurately adjust the bacterial concentration according to experimental requirements. Precise control of the collected colony quantity allows for the convenient preparation of bacterial solutions with different concentration gradients, which is significant for studying the growth characteristics of microorganisms and the effects of drugs on microorganisms. The novel inoculation needle design facilitates automation during operation, contributing to improved laboratory efficiency and accuracy.

[0029] See Figs. 1-3 As shown, an inoculation needle for quantitative colony collection according to an embodiment of this application includes:

[0030] A sampling needle 1 has a groove 2 inside one end and an opening 3 at the end of the sampling needle 1 near the groove 2, the opening 3 communicating with the groove 2. A connection port 4 is provided on the side wall of the end of the sampling needle 1 near the groove 2, the connection port 4 communicating with the groove 2. Anti-slip holes 5 are provided on the inner wall of the connection port 4, the inner wall of the groove 2, and the end of the sampling needle 1 near the groove 2. A fiber layer is provided on the surface of the anti-slip holes 5.

[0031] A scraping tube 6 is provided on the outer wall of the sampling needle 1, and the inner wall of the scraping tube 6 is in sliding connection with the outer wall of the sampling needle 1.

[0032] In some embodiments, the transverse cross-section of the sampling needle 1 is a circular structure.

[0033] It should be understood that the circular cross-section uniformly distributes stress when subjected to external forces, effectively disperses pressure, and reduces stress concentration. This structure makes the inoculation needle less likely to deform or break during use, thereby improving its mechanical strength and durability. The smooth surface of the circular cross-section has no corners or depressions, reducing the likelihood of microbial attachment. During the experiment, colonies are more easily removed by the scraping tube, avoiding cross-contamination caused by colony residue. At the same time, the smooth surface also facilitates cleaning and disinfection, improving the accuracy and repeatability of the experiment.

[0034] In some embodiments, the outer diameter of the middle part of the groove 2 is greater than the outer diameter of the two ends of the groove 2.

[0035] In some embodiments, the groove 2 coincides with the axis of the sampling needle 1.

[0036] In some embodiments, the opening 3 coincides with the axis of the groove 2, and the transverse cross-section of the opening 3 is a circular structure.

[0037] In some embodiments, the transverse cross-section of the connection port 4 is a sector structure.

[0038] In some embodiments, the connection port 4 is arranged in a ring array around the opening 3.

[0039] In some embodiments, the connection port 4 communicates with the opening 3.

[0040] In some embodiments, the inner diameter of the opening 3 is smaller than the outer diameter of the middle part of the groove 2.

[0041] It should be understood that the larger outer diameter of the middle part of the groove 2 provides more space for adsorption and aggregation of colonies. During inoculation, colonies are more easily guided to the middle part of the groove 2 and effectively adsorbed and retained. Compared to the narrower parts at both ends of the groove, the wider design of the middle part reduces the risk of colony loss due to flow or vibration during inoculation, thereby improving the efficiency of colony collection. The opening 3, as the main channel for colonies to enter the groove 2, coincides with the axis of the groove to ensure smooth flow of colonies into the groove, reducing flow resistance and possible blockage. The circular cross-section of the opening helps to evenly distribute colonies in the middle part of the groove 2, avoiding uneven deposition or aggregation of colonies due to irregular cross-sectional shape, improving the uniformity and accuracy of colony collection.

[0042] In some embodiments, the inner diameter of the scraper tube 6 matches the outer diameter of the sampling needle 1.

[0043] It should be understood that when the inner diameter of the scraper tube 6 closely matches the outer diameter of the sampling needle 1, the scraper tube can precisely fit on the outer sidewall of the sampling needle, ensuring that the gap between the scraper tube and the sampling needle is minimized during rotation or sliding, thereby accurately scraping off the excess colonies on the sampling needle. This precise matching relationship helps to reduce the situation that the colonies are not completely removed due to a too large gap, or the colonies are damaged due to a too small gap, thereby improving the accuracy and reliability of the experimental results. The design of matching the inner diameter with the outer diameter makes the scraper tube more stable when it is sleeved on the sampling needle, and is not easy to shake or shift, which is convenient for the operator to operate and control. Due to the close matching relationship between the scraper tube and the sampling needle, the scraping effect of the scraper tube on the colonies is more consistent in each experiment, which helps to improve the repeatability and comparability of the experiment.

[0044] Obviously, those skilled in the art can make various modifications and variations to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalent technologies, the present application also intends to include these modifications and variations.

Claims

1. A swabbing needle for quantitative collection of colonies, characterized in that, The utility model relates to a sampling needle and scraping tube, which comprises a sampling needle and a scraping tube. The sampling needle has a groove in the inner part of one end, an opening in the end close to the groove, a connecting port in the side wall of the end close to the groove, and an anti-skid hole in the inner wall of the connecting port, the inner wall of the groove and the end close to the groove. The scraping tube is sleeved on the outer side wall of the sampling needle and is in sliding connection with the outer side wall of the sampling needle.

2. The inoculation needle for quantitative collection of bacterial colonies according to claim 1, characterized in that The transverse section of the sampling needle is in a circular structure.

3. The inoculation needle for quantitative collection of bacterial colonies according to claim 1, characterized in that, The outer diameter of the middle part of the groove is larger than that of the two ends of the groove.

4. The inoculation needle for quantitative collection of bacterial colonies according to claim 3, characterized in that The groove coincides with the axis of the sampling needle.

5. The inoculation needle for quantitative collection of bacterial colonies according to claim 4, characterized in that The opening coincides with the axis of the groove, and the transverse section of the opening is in a circular structure.

6. The inoculation needle for quantitative collection of bacterial colonies according to claim 1, characterized in that, The transverse section of the connecting port is in a sector structure.

7. The inoculation needle for quantitative collection of bacterial colonies according to claim 6, characterized in that The connecting port is arranged in a plurality of annular arrays with the opening as the axis.

8. The inoculation needle for quantitative collection of bacterial colonies according to claim 7, characterized in that The plurality of connecting ports are in communication with the opening.

9. The inoculating needle for quantitative collection of bacterial colonies according to claim 3, characterized in that, The inner diameter of the opening is smaller than the outer diameter of the middle part of the groove.

10. The inoculation needle for quantitative collection of bacterial colonies according to claim 1, characterized in that The inner diameter of the scraping tube matches the outer diameter of the sampling needle.

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