Immunoaffinity detection device for mycotoxin

Abstract

The utility model relates to the technical field of food detection, in particular to an immunoaffinity detection device for fungaltoxin, which can push, mix and stir test liquid, is simpler to operate and is favorable for improving the working efficiency. Comprising a column tube, a liquid outlet and a tube cover, the funnel is installed in the middle of the cylindrical cavity of the column pipe, a through hole is formed in the middle of the funnel, the inner rod is vertically installed on the upper middle portion of the cylindrical cavity of the column pipe, the upper end of the inner rod extends out of the outside above the pipe cover, the piston is installed at the lower end of the inner rod, and the piston seals the through hole of the funnel from the lower portion. The inner rod is sleeved with the pushing pipe, the upper end of the pushing pipe extends out of the upper portion of the pipe cover, the impeller is rotationally installed on the inner wall of the cylindrical cavity of the column pipe and provided with a plurality of inclined blades, a sleeve is arranged in the middle of the impeller, and the lower end of the pushing pipe is in transmission connection with the impeller sleeve.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of food detection, in particular to a kind of for mycotoxin's immune affinity detection device. BACKGROUND

[0002] Mycotoxin is harmful secondary metabolite produced by aspergillus, fusarium and other fungi in production process, and some mycotoxin has serious influence on human and animal health. The prior art with publication number CN212255354U discloses a kind of multi-in-one mycotoxin immune affinity column with adjustable blade, the mycotoxin immune affinity column tube is built-in push-pull piece, after adding test liquid to affinity column, gently pull push handle, outer blade and inner blade are separated, liquid flows through funnel hole of outer blade and funnel hole of inner blade in turn, enters gel layer, horizontally rotates 45-90 degrees to push handle of push-pull piece, outer blade funnel hole and inner blade funnel hole are horizontally staggered, push handle downward, outer blade and inner blade form closed blade, can discharge liquid in column along liquid outlet, pull up push handle, outer blade and inner blade are separated, and test solution can be added again. The inner edge of hollow connecting rod has protrusion, the protrusion cooperates with blocking piece, forms effective connection between inner blade and outer blade. Outer blade and inner blade both have funnel hole, filler layer liquid cannot move upward, gas cannot enter filler layer along liquid outlet to form bubble, effectively avoid the interference of bubble to affinity chromatography effect.

[0003] But the immune affinity detection device for mycotoxin of above-mentioned prior art needs to rotate push-pull piece to make funnel hole of inner and outer blade align or stagger when pushing test liquid with inner and outer blade, operation is more complex, not conducive to improving work efficiency. UTILITY MODEL CONTENT

[0004] To solve the above technical problems, the utility model provides an immune affinity detection device for mycotoxin, which can push and mix test liquid, is more simple to operate, and is conducive to improving work efficiency.

[0005] The utility model discloses an immune affinity detection device for mycotoxin, including the column pipe, liquid outlet and the pipe cover, the inside of column pipe is provided column cavity, and the bottom of column cavity is filled with filler, and the lower extreme of column pipe is provided with the opening of intercommunication with column cavity, and the upper port of column pipe is closed by the pipe cover, still include the hopper, inner rod, piston, push pipe and impeller, the middle part of hopper is installed in the column cavity of column pipe, and the middle part of hopper is provided with through -hole, and inner rod is vertically installed in the upper part of column cavity of column pipe, and the upper end of inner rod is stretched out the upper outside of pipe cover, and the lower end of inner rod installs piston, and the through -hole of hopper is closed from below by piston, and push pipe is sleeved in the outside of inner rod, and the upper end of push pipe is stretched out the upper outside of pipe cover, and impeller is rotatably installed on the inner wall of column cavity of column pipe, and impeller is provided with multiple inclined blades, and the middle part of impeller is provided with sleeve, and the lower end of push pipe is transmission connection with impeller sleeve, impeller sets up multiple, and multiple impellers are installed in the upper part of column cavity of column pipe in series, when working, the test liquid is inputed in the space above the hopper of column cavity through the upper port of liquid outlet, and the upper port of column pipe is closed through the pipe cover, and the inner rod is pushed down, and the lower end of inner rod pushes down piston, and the through -hole of hopper is opened, and the push pipe drives impeller to rotate, and multiple inclined blades of impeller push down test liquid and guide, thereby make test liquid accelerate and flow down through the through -hole of hopper, and make test liquid contact with the filler of the lower part of column cavity, when the test liquid on the filler is transported to enough amount, lift inner rod, make piston close the through -hole of hopper, make test liquid react with the filler, compared with prior art, can push test liquid and also can mix and stir test liquid, and need repeatedly adjust the opening and closing of through -hole, therefore, operation is simpler, and it is favorable for improving work efficiency.

[0006] Preferably, it further comprises a plurality of push rods and a plurality of sliding grooves, a plurality of push rods are installed on the outer wall of the inner rod and the push tube, and a plurality of sliding grooves are arranged on the inner wall of the sleeve of the impeller, and the plurality of push rods are slidably connected with the plurality of sliding grooves; the plurality of push rods are slidably connected with the plurality of sliding grooves, so that the push tube is transmission connected with the impeller, and the structure is simple and reliable.

[0007] Preferably, the plurality of sliding grooves are spiral; repeatedly lifting and pressing the push tube, so that the push tube drives the plurality of push rods to extrude the plurality of spiral sliding grooves, and then the push tube drives the impeller to rotate reciprocatingly through repeated extension and contraction.

[0008] Preferably, it further comprises a spring, the upper end of the spring is connected with the lower end of the push tube, and the lower end of the spring is connected with the hopper; the spring elastically supports the push tube, so that when the push tube is loosened, the elastic force of the spring pushes the push tube upward to reset, which facilitates the push tube to drive the impeller to rotate repeatedly through the plurality of push rods, and simplifies the operation.

[0009] Preferably, the plurality of spring pieces are connected to the lower end of the piston, and the lower end of the plurality of spring pieces is connected to the inner wall of the cylindrical chamber of the column pipe, and the plurality of spring pieces are arc-shaped; when the test liquid enters the funnel and contacts the filler, the inner rod is reciprocated to press, the piston is reciprocated up and down, the plurality of spring pieces are continuously changed in bending curvature to stir the test liquid, and the contact efficiency of the test liquid and the filler is improved.

[0010] Preferably, the large-hole filter plate and the small-hole filter plate are arranged, the large-hole filter plate is arranged at the bottom of the cylindrical chamber of the column pipe, the small-hole filter plate is arranged at the lower part of the cylindrical chamber of the column pipe, and the large-hole filter plate and the small-hole filter plate are used for filling the filler; the large-hole filter plate and the small-hole filter plate are used for loading the filler, the filler is contacted with the test liquid above the small-hole filter plate through the filter holes of the small-hole filter plate to react, and after the reaction is completed, the inner rod is pushed downward, the piston is lowered, the plurality of spring pieces are bent downward to push the small-hole filter plate downward, and the filler is discharged through the filter holes of the large-hole filter plate and the liquid outlet.

[0011] Compared with the prior art, the beneficial effects of the utility model are that the test liquid can be pushed and mixed and stirred at the same time, the opening and closing of the through hole need to be repeatedly adjusted, the operation is simpler, and the working efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0012] Figure 1 is a structural schematic diagram of the utility model;

[0013] Figure 2 is a front cut structure schematic diagram of the utility model;

[0014] Figure 3 is an isometric structural schematic diagram of the utility model;

[0015] Figure 4 is a structural schematic diagram of the funnel, the pushing pipe, the impeller, the stirring rod and the spring in the exploded state;

[0016] Figure 5 is a structural schematic diagram of the funnel, the inner rod, the piston and the spring piece.

[0017] Markings in the drawings: 1, column pipe; 2, liquid outlet; 3, pipe cover; 4, funnel; 5, inner rod; 6, piston; 7, pushing pipe; 8, impeller; 9, stirring rod; 10, sliding groove; 11, spring; 12, spring piece; 13, large-hole filter plate; 14, small-hole filter plate. DETAILED DESCRIPTION

[0018] For the purpose of facilitating the understanding of the present application, the present application will be described more fully below with reference to the accompanying drawings. The present application can be realized in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive. Embodiment 1

[0019] As Figures 1 to 4 shown, an immunoaffinity detection device for mycotoxin comprises a column tube 1, a liquid outlet 2 and a tube cover 3, the inside of the column tube 1 is provided with a cylindrical chamber, the bottom of the cylindrical chamber is filled with a filler, the lower end of the column tube 1 is provided with an opening communicating with the cylindrical chamber, and the upper end of the column tube 1 is closed by the tube cover 3; further comprising a funnel 4, an inner rod 5, a piston 6, a push tube 7 and an impeller 8, the funnel 4 is installed in the middle of the cylindrical chamber of the column tube 1, the middle of the funnel 4 is provided with a through hole, the inner rod 5 is vertically installed in the upper middle of the cylindrical chamber of the column tube 1, the upper end of the inner rod 5 extends out of the upper external environment of the tube cover 3, the lower end of the inner rod 5 is installed with the piston 6, the piston 6 closes the through hole of the funnel 4 from below, the push tube 7 is sleeved on the outer side of the inner rod 5, the upper end of the push tube 7 extends out of the upper external environment of the tube cover 3, and the impeller 8 is rotationally installed on the inner wall of the cylindrical chamber of the column tube 1; the impeller 8 is provided with a plurality of inclined blades, a sleeve is arranged in the middle of the impeller 8, and the lower end of the push tube 7 is in transmission connection with the sleeve of the impeller 8.

[0020] The impeller 8 is provided with a plurality of impellers 8, and the plurality of impellers 8 are installed in series in the upper middle of the cylindrical chamber of the column tube 1; in working, the test liquid is input into the space above the funnel 4 in the cylindrical chamber of the column tube 1 through the upper end of the liquid outlet 2, the upper end of the column tube 1 is closed by the tube cover 3, the inner rod 5 is pushed downward, the lower end of the inner rod 5 pushes the piston 6 downward, the through hole of the funnel 4 is opened, the push tube 7 drives the impeller 8 to rotate, the plurality of inclined blades of the impeller 8 push and guide the test liquid downward, so that the test liquid flows downward through the through hole of the funnel 4 at a faster speed, and the test liquid contacts with the filler in the lower part of the cylindrical chamber of the column tube 1; when the test liquid on the filler is transported to a sufficient amount, the inner rod 5 is lifted, the piston 6 closes the through hole of the funnel 4, the test liquid reacts with the filler, compared with the prior art, the test liquid can be pushed and mixed and stirred at the same time, and the opening and closing of the through hole need to be repeatedly adjusted, so that the operation is simpler, and the work efficiency is improved. Embodiment 2

[0021] As Figure 1 , Figure 2 and Figure 4As shown, based on Embodiment 1, it also includes multiple levers 9 and multiple sliding grooves 10. Multiple levers 9 are installed on the outer wall of the inner rod 5 and the push tube 7. Multiple sliding grooves 10 are provided on the inner wall of the sleeve of the impeller 8. The multiple levers 9 are slidably connected to the multiple sliding grooves 10 respectively. The multiple sliding grooves 10 are spiral. It also includes a spring 11. The upper end of the spring 11 is connected to the lower end of the push tube 7, and the lower end of the spring 11 is connected to the funnel 4.

[0022] Multiple levers 9 are slidably connected to multiple grooves 10, thereby driving the push tube 7 to the impeller 8. The structure is simple and reliable. Repeatedly lifting and pressing the push tube 7 causes the push tube 7 to drive the multiple levers 9 to squeeze the multiple spiral grooves 10 respectively. In turn, the push tube 7 drives the impeller 8 to rotate back and forth by repeatedly extending and retracting the push tube 7. The spring 11 elastically supports the push tube 7, so that when the push tube 7 is released, the elastic force of the spring 11 pushes the push tube 7 upward to reset. This makes it convenient for the push tube 7 to drive the impeller 8 to rotate repeatedly through the multiple levers 9, simplifying the operation. Example 3

[0023] like Figure 1 , Figure 2 and Figure 5 As shown, based on Embodiment 1, it also includes multiple spring plates 12, the upper ends of which are connected to the lower end of the piston 6, and the lower ends of which are connected to the inner wall of the cylindrical cavity of the column tube 1. The multiple spring plates 12 are arc-shaped. It also includes a large-pore filter plate 13 and a small-pore filter plate 14. The large-pore filter plate 13 is installed at the bottom of the cylindrical cavity of the column tube 1, and the small-pore filter plate 14 is installed at the lower part of the cylindrical cavity of the column tube 1. The space between the large-pore filter plate 13 and the small-pore filter plate 14 is used to fill the gap with packing material.

[0024] When the test liquid enters the funnel 4 and contacts the packing, the inner rod 5 is pressed repeatedly, causing the piston 6 to move up and down. This causes the piston 6 to push multiple spring plates 12 to continuously change their bending arc, thereby agitating the test liquid and improving the contact efficiency between the test liquid and the packing. The large-pore filter plate 13 and the small-pore filter plate 14 load the packing. The packing reacts with the test liquid above the small-pore filter plate 14 through the filter holes of the small-pore filter plate 14. After the reaction is completed, the inner rod 5 is pushed down, causing the piston 6 to descend and the multiple spring plates 12 to bend downward, pushing the small-pore filter plate 14 downward and discharging the packing through the filter holes of the large-pore filter plate 13 and the outlet 2.

[0025] like Figures 1 to 5As shown, the utility model discloses an immune affinity detection device for mycotoxin, when working, first, the test liquid is input into the space above funnel 4 in the cylindrical chamber of column pipe 1 through the upper port of liquid outlet 2, the upper port of column pipe 1 is closed through pipe cover 3, then push down inner rod 5, the lower end of inner rod 5 pushes down piston 6, the through hole of funnel 4 is opened, drive impeller 8 to rotate through push pipe 7, a plurality of inclined blades of impeller 8 push down test liquid and guide, thereby make test liquid flow down through the through hole of funnel 4, make test liquid contact with the filler in the lower part of the cylindrical chamber of column pipe 1 through the filter hole of small hole filter plate 14, then when the test liquid on the filler is transported to enough amount, reciprocating press inner rod 5, make piston 6 reciprocate, make piston 6 push a plurality of spring leaves 12 to change the bending amplitude ceaselessly, thereby make a plurality of spring leaves 12 stir test liquid, make test liquid react with the filler, finally, when the reaction is completed, push down inner rod 5, make piston 6 descend and make a plurality of spring leaves 12 bend down, push down small hole filter plate 14, make the filler discharge through the filter hole of big hole filter plate 13 and liquid outlet 2.

[0026] The utility model realizes the main function:

[0027] 1, can push and mix the test liquid and stir, operation is simpler, is favorable for improving work efficiency,

[0028] 2, through a plurality of spring leaves 12 stir test liquid above the filler, improve the contact efficiency of test liquid and filler,

[0029] 3, through the upper and lower filter plate constraint filler, and can push down the filler and discharge, convenient operation.

[0030] The utility model discloses an immune affinity detection device for mycotoxin, its installation mode, connecting mode or setting mode are all common mechanical mode, as long as can reach its beneficial effect can be implemented, the column pipe 1 of the utility model discloses an immune affinity detection device for mycotoxin, liquid outlet 2, pipe cover 3, funnel 4, piston 6, impeller 8, spring 11, spring leaf 12, big hole filter plate 13, small hole filter plate 14 are purchased on the market, and the technical personnel in this industry only need to install and operate according to its attached instruction book, and need not the technical personnel of this field to pay the creative labor.

[0031] All the technical and scientific terms used in the present application have the same meaning as understood by the person skilled in the art to which the present application belongs. The terms used in the specification of the present application are only for the purpose of describing the specific embodiments and are not intended to limit the present application. The term "and / or" used in the present application includes any and all combinations of one or more related listed items.

[0032] The above merely describes preferred embodiments of the present application, and it should be noted that, for those skilled in the art, without departing from the technical principles of the present application, a number of improvements and modifications can be made, and these improvements and modifications should also be considered as the protection scope of the present application.

Claims

1. An immunoaffinity detection device for mycotoxins, comprising a column tube (1), an outlet (2), and a cap (3), wherein a cylindrical chamber is provided inside the column tube (1), the bottom of the cylindrical chamber is filled with packing material, an opening communicating with the cylindrical chamber is provided at the lower end of the column tube (1), and the cap (3) closes the upper end of the column tube (1); characterized in that, It also includes a funnel (4), an inner rod (5), a piston (6), a push tube (7), and an impeller (8). The funnel (4) is installed in the middle of the cylindrical chamber of the column tube (1). A through hole is provided in the middle of the funnel (4). The inner rod (5) is installed vertically in the upper part of the cylindrical chamber of the column tube (1). The upper end of the inner rod (5) extends out of the outside of the tube cover (3). The piston (6) is installed at the lower end of the inner rod (5). The piston (6) closes the through hole of the funnel (4) from below. The push tube (7) is sleeved on the outside of the inner rod (5). The upper end of the push tube (7) extends out of the outside of the tube cover (3). The impeller (8) is rotatably installed on the inner wall of the cylindrical chamber of the column tube (1). The impeller (8) is provided with multiple inclined blades. A sleeve is provided in the middle of the impeller (8). The lower end of the push tube (7) is connected to the sleeve of the impeller (8) for transmission.

2. The immunoaffinity detection device for mycotoxins as described in claim 1, characterized in that, It also includes multiple levers (9) and multiple slides (10), an inner rod (5), multiple levers (9) are installed on the outer wall of the push tube (7), multiple slides (10) are provided on the inner wall of the sleeve of the impeller (8), and multiple levers (9) are slidably connected to multiple slides (10) respectively.

3. The immunoaffinity detection device for mycotoxins as described in claim 2, characterized in that, Multiple grooves (10) are spiral-shaped.

4. The immunoaffinity detection device for mycotoxins as described in claim 3, characterized in that, It also includes a spring (11), the upper end of which is connected to the lower end of the push tube (7), and the lower end of which is connected to the funnel (4).

5. The immunoaffinity detection device for mycotoxins as described in claim 1, characterized in that, It also includes multiple spring plates (12), the upper ends of the multiple spring plates (12) are connected to the lower end of the piston (6), the lower ends of the multiple spring plates (12) are connected to the inner wall of the cylindrical cavity of the column tube (1), and the multiple spring plates (12) are arc-shaped.

6. The immunoaffinity detection device for mycotoxins as described in claim 5, characterized in that, It also includes a macroporous filter plate (13) and a microporous filter plate (14). The macroporous filter plate (13) is installed at the bottom of the cylindrical chamber of the column tube (1), and the microporous filter plate (14) is installed at the lower part of the cylindrical chamber of the column tube (1). The space between the macroporous filter plate (13) and the microporous filter plate (14) is used to fill the packing material.

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