Multicolor excitation fluorescence microscope

Abstract

The utility model discloses a multicolor excitation fluorescence microscope, and belongs to the field of microscopes. Comprising a first numerical control light source; the carrying seat is arranged below the first numerical control light source; the opening is formed in the carrying plate; the carrying plate is arranged on the carrying seat; the numerical control objective lens converter is arranged below the loading plate, and the numerical control objective lens converter corresponds to the opening; the chroscope box is connected to the interface of the numerical control objective lens converter; the dichroscope is movably arranged in the dichroscope box; the X-ray plate box is arranged at the bottom of the color mirror box; the movable sheet is arranged on the X-ray plate box in a sliding manner; the excitation optical filter is movably arranged on the moving sheet; the second numerical control light source is arranged at the bottom of the light sheet box and corresponds to the excitation light filter; and the high-definition automatic focusing camera is connected with the interface of the chroscope box. The beneficial effect of the utility model is that the multicolor excitation fluorescence microscope is provided.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microscope, in particular, to a multi-color excitation fluorescence microscope. BACKGROUND

[0002] Microscope is a kind of precision optical instrument, which has a history of more than 300 years. Since the microscope was invented, people have seen many microorganisms and basic units that constitute the organism, which makes us have a further understanding of the law of life activities of organisms. In the experiments specified in the biological teaching syllabus, most of them are completed through the microscope. Therefore, the performance of the microscope is one of the key factors for doing a good job in the experiment. However, the existing device still has some defects.

[0003] When switching between ordinary optical microscope and fluorescence microscope, dichroic mirror box is needed, and dichroic mirror is installed in the dichroic mirror box to realize switching. When different fluorescence dye samples need to be switched, different excitation filters need to be switched. However, in the prior art, the excitation filter needs to be disassembled and reassembled, which makes it troublesome to switch the excitation filter.

[0004] Now a multi-color excitation fluorescence microscope is provided. CONTENT OF THE INVENTION

[0005] The summary part of the present application is used to introduce the concept in a simple form, which will be described in detail in the specific embodiment part. The summary part of the present application is not intended to identify the key features or essential features of the claimed technical solution, nor is it intended to limit the scope of the claimed technical solution.

[0006] In order to solve the technical problems mentioned in the background part, some embodiments of the present application provide a multi-color excitation fluorescence microscope, comprising: a first numerical control light source; a stage, arranged below the first numerical control light source; an opening, opened on the stage plate; a stage plate, arranged on the stage; a numerical control objective lens converter, arranged below the stage plate, and the numerical control objective lens converter corresponds to the opening; a color mirror box, connected at the interface of the numerical control objective lens converter; a dichroic mirror, movably arranged in the color mirror box; a filter box, arranged at the bottom of the color mirror box; a moving piece, slidably arranged on the filter box; an excitation filter, movably arranged on the moving piece; a second numerical control light source, arranged at the bottom of the filter box and corresponding to the excitation filter; and a high-definition automatic focusing camera, connected at the interface of the color mirror box.

[0007] When detection is needed, the first numerical control light source is turned on, the slide is placed on the objective slot, and the corresponding objective head is switched to scan the image through the high-definition automatic focusing camera and transmit it to the upper computer for analysis. The statistical data and analysis results are formed into a report for the staff to check. The detection method is the prior art. When different objective heads need to be switched, the conversion motor is started to rotate the output shaft of the conversion motor to switch different objective heads. When the fluorescence microscope needs to be switched, the dichroic mirror is installed in the color mirror box and connected to the color mirror box through the mounting bolt to realize the installation of the dichroic mirror. Under the action of the excitation filter and the second numerical control light source, the fluorescence dyed sample can be observed. When different fluorescence dyed samples need to be observed, the excitation filter is placed on the mounting slot and limited by the lap joint groove. The moving piece is pulled through the pull hole to move the excitation filter, so that different excitation filters enter the filter box to realize the switching of different fluorescence dyed samples. The operation is simple and does not need to be disassembled.

[0008] In some embodiments, a plurality of mounting slots are uniformly arranged on the moving piece, and a lap joint groove is arranged on the inner wall of the mounting slot. The excitation filter corresponds to the mounting slot and is movably arranged on the lap joint groove.

[0009] In some embodiments, a pull hole is arranged on the moving piece.

[0010] In some embodiments, the dichroic mirror is fixedly connected with a connecting plate, the connecting plate is in contact with the color mirror box, and threaded holes are arranged on the connecting plate and the filter box.

[0011] In some embodiments, the numerical control objective converter comprises a shell, a conversion motor, an objective seat, and a plurality of objective heads. The conversion motor is arranged on the shell, the objective heads are arranged on the objective seat, and the output shaft of the conversion motor is connected with the objective seat.

[0012] In some embodiments, the bottom of the high-definition automatic focusing camera is provided with a mounting seat, and a fixing bolt is threadedly connected to the mounting seat.

[0013] In some embodiments, the fixing bolt is provided as four, and is threadedly connected at four corners of the mounting seat.

[0014] The application has the beneficial effect of providing a multi-color excitation fluorescence microscope. BRIEF DESCRIPTION OF DRAWINGS

[0015] The accompanying drawings, which form a part of this application, are intended to provide further understanding of the application and are incorporated herein in their entirety. The drawings included are schematic representations of exemplary embodiments of the application, and are not limiting of the scope of the application.

[0016] In addition, throughout the drawings, like reference numerals are used to designate like elements. It should be understood that the drawings are schematic and elements and features are not necessarily to scale.

[0017] In the drawings:

[0018] Figure 1 is a schematic view of the entire embodiment according to the present application;

[0019] Figure 2 is a schematic view of the entire embodiment according to the present application from another perspective;

[0020] Figure 3 is a schematic view of the entire embodiment according to the present application from another perspective;

[0021] Figure 4 is a sectional view of the entire embodiment according to the present application;

[0022] Figure 5 is a sectional view of the color mirror box and the light sheet box;

[0023] Figure 6 is a schematic view of the light sheet box according to the present application;

[0024] Figure 7 is a schematic view of the moving sheet according to the present application.

[0025] Reference numerals:

[0026] 1, first numerical control light source; 2, object carrier; 3, object plate; 4, shell; 5, color mirror box; 6, light sheet box; 7, second numerical control light source; 8, connecting plate; 9, moving sheet; 10, mounting seat; 11, opening; 12, high-definition automatic focusing camera; 13, threaded hole; 14, mounting groove; 15, pull hole; 16, dichroic mirror; 17, lap joint groove; 18, excitation filter; 19, objective lens; 20, objective lens seat; 21, conversion motor; 22, numerical control objective lens converter; 23, fixing bolt. DETAILED DESCRIPTION

[0027] Embodiments of the present disclosure will be described in greater detail below with reference to the accompanying drawings. While certain embodiments of the present disclosure are shown in the drawings, it is understood that the present disclosure can be embodied in various forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that the present disclosure will be thoroughly understood. It should be understood that the drawings and embodiments of the present disclosure are for illustrative purposes only and are not intended to limit the scope of the present disclosure.

[0028] In addition, it needs to be noted that only parts related to the present application are shown in the drawings for the convenience of description. The embodiments in the present disclosure and the features in the embodiments can be combined with each other without conflict.

[0029] It should be noted that the terms "first", "second" and the like mentioned in the present disclosure are only used to distinguish different devices, modules or units, and are not intended to limit the order or interdependence of the functions performed by these devices, modules or units.

[0030] It should be noted that the modification of "one" or "multiple" mentioned in the present disclosure is illustrative but not restrictive, and those skilled in the art should understand that unless otherwise explicitly indicated in the context, it should be understood as "one or more".

[0031] The present disclosure will be described in detail below with reference to the drawings and in conjunction with embodiments.

[0032] Referring to Figures 1-7 A multi-color excitation fluorescence microscope comprises a first numerical control light source 1, a stage 2, an opening 11, a carrier plate 3, a numerical control objective converter 22, a color mirror box 5, a dichroic mirror 16, a light sheet box 6, a moving sheet 9, an excitation filter 18, a second numerical control light source 7, and a high-definition autofocus camera 12. The stage 2 is arranged below the first numerical control light source 1. The opening 11 is formed in the carrier plate 3. The carrier plate 3 is arranged on the stage 2. The carrier plate 3 has a carrier groove at the top, and the carrier groove is used to place a glass slide. The numerical control objective converter 22 is arranged below the carrier plate 3 and corresponds to the opening 11. The color mirror box 5 is connected to the interface of the numerical control objective converter 22. The dichroic mirror 16 is movably arranged in the color mirror box 5. The light sheet box 6 is arranged at the bottom of the color mirror box 5. According to whether the dichroic mirror 16 is arranged in the color mirror box 5, the ordinary optical microscope and the fluorescence microscope can be switched. The moving sheet 9 is slidably arranged on the light sheet box 6. The excitation filter 18 is movably arranged on the moving sheet 9. The second numerical control light source 7 is arranged at the bottom of the light sheet box 6 and corresponds to the excitation filter 18. The excitation filter 18 cooperates to realize the viewing of a fluorescently dyed sample, and different excitation filters 18 are switched to realize the viewing of different fluorescently dyed samples. The high-definition autofocus camera 12 is connected to the interface of the color mirror box 5.

[0033] A plurality of mounting grooves 14 are uniformly formed in the moving sheet 9, and a lap joint groove 17 is formed in the inner wall of the mounting groove 14. The excitation filter 18 corresponds to the mounting groove 14 and is movably arranged in the lap joint groove 17. A pull hole 15 is formed in the moving sheet 9.

[0034] A connecting plate 8 is fixedly connected to the dichroic mirror 16, and the connecting plate 8 is in contact with the color mirror box 5. Threaded holes 13 are formed in the connecting plate 8 and the light sheet box 6.

[0035] The numerical control objective converter 22 comprises a shell 4, a conversion motor 21, an objective seat 20, and a plurality of objective lenses 19. The conversion motor 21 is arranged on the shell 4, the objective lenses 19 are arranged on the objective seat 20, and the output shaft of the conversion motor 21 is connected with the objective seat 20. When it is necessary to switch the objective lenses 19 of different magnifications, the conversion motor 21 can be started, so that the output shaft of the conversion motor 21 drives the objective seat 20 to rotate, thereby switching the objective lenses 19 of different magnifications.

[0036] The bottom of the high-definition automatic focusing camera 12 is provided with a mounting seat 10, and the mounting seat 10 is threadedly connected with fixing bolts 23. The fixing bolts 23 are arranged at four corners of the mounting seat 10.

[0037] Working process: when detection is needed, the first numerical control light source 1 is turned on, the slide glass is placed on the object slot, and the objective lens 19 of the corresponding magnification is switched. The image scanned by the high-definition automatic focusing camera 12 is transmitted to the upper computer for analysis in a wireless mode. The statistical data and analysis results are formed into a report for the staff to check through intelligent identification. The detection method is the prior art. When it is necessary to switch the objective lenses 19 of different magnifications, the conversion motor 21 can be started, so that the output shaft of the conversion motor 21 drives the objective seat 20 to rotate, thereby switching the objective lenses 19 of different magnifications. When it is necessary to switch the fluorescence microscope, the dichroic mirror 16 is mounted into the color mirror box 5, and the connecting plate 8 is connected to the color mirror box 5 through the mounting bolt, thereby achieving the mounting of the dichroic mirror 16. Under the action of the excitation filter 18 and the second numerical control light source 7, the viewing of the fluorescence dyed sample is achieved. When it is necessary to switch the viewing of different fluorescence dyed samples, the excitation filter 18 is placed on the mounting groove 14, and the excitation filter 18 is limited through the action of the lap joint groove 17. The moving piece 9 is pulled through the pull hole 15, thereby driving the excitation filter 18 to move, so that the different excitation filters 18 enter into the filter box 6, thereby achieving the switching of different fluorescence dyed samples. The operation is simple, and the excitation filter 18 does not need to be disassembled.

[0038] The above description is only some of the preferred embodiments of the present disclosure and the explanation of the applied technical principles. Those skilled in the art should understand that the application range involved in the embodiments of the present disclosure is not limited to the technical solutions formed by the specific combinations of the above technical features, and should also cover other technical solutions formed by any combination of the above technical features or equivalent features without departing from the above inventive concept. For example, the above features are replaced with the technical features disclosed in the embodiments of the present disclosure (but not limited to) having similar functions to form technical solutions.

Claims

1. A multi-color excitation fluorescence microscope, comprising: a first numerical control light source; characterized in that the multi-color excitation fluorescence microscope further comprises: a stage, arranged below the first numerical control light source; an opening, provided on the stage plate; a stage plate, arranged on the stage; a numerical control objective lens converter, arranged below the stage plate, and the numerical control objective lens converter corresponds to the opening; a dichroic mirror box, connected to the interface of the numerical control objective lens converter; a dichroic mirror, movably arranged in the dichroic mirror box; a light sheet box, arranged at the bottom of the dichroic mirror box; a moving sheet, slidably arranged on the light sheet box; an excitation filter, movably arranged on the moving sheet; a second numerical control light source, arranged at the bottom of the light sheet box and corresponding to the excitation filter; a high-definition automatic focusing camera, connected to the interface of the dichroic mirror box.

2. The multi-color excitation fluorescence microscope according to claim 1, characterized in that: a plurality of mounting grooves are uniformly provided on the moving sheet, and a lap joint groove is provided on the inner wall of the mounting groove, and the excitation filter corresponds to the mounting groove and is movably arranged on the lap joint groove.

3. The multi-color excitation fluorescence microscope according to claim 2, characterized in that: a pull hole is provided on the moving sheet.

4. The multi-color excitation fluorescence microscope according to claim 1, characterized in that: a connecting plate is fixedly connected to the dichroic mirror, and the connecting plate is in contact with the dichroic mirror box, and threaded holes are provided on the connecting plate and the light sheet box.

5. The multi-color excitation fluorescence microscope according to claim 1, characterized in that: the numerical control objective lens converter comprises a housing, a conversion motor, an objective lens seat, and a plurality of objective lens heads, the conversion motor is arranged on the housing, the objective lens heads are arranged on the objective lens seat, and the output shaft of the conversion motor is connected with the objective lens seat.

6. The multi-color excitation fluorescence microscope according to claim 1, characterized in that: a mounting seat is arranged at the bottom of the high-definition automatic focusing camera, and a fixing bolt is threadedly connected to the mounting seat.

7. The multi-color excitation fluorescence microscope according to claim 6, characterized in that: the fixing bolt is provided as four, and is threadedly connected at four corners of the mounting seat.

Images