Simple manual sample adding auxiliary device for microwell plate

Abstract

The utility model discloses a simple auxiliary device for manual sample adding of a microplate, which relates to the technical field of manual sample adding of microplates and comprises a device body used for being mounted on the microplate, a plurality of cell culture holes are formed in the microplate, a mounting plate cover is movably modified on the microplate, the device body comprises an auxiliary sample adding plate cover, and the auxiliary sample adding plate cover is movably mounted on the mounting plate cover. The sample adding auxiliary plate cover and the microwell plate are detachably mounted, a plurality of rows of sample adding auxiliary round holes distributed at equal intervals are formed in the sample adding auxiliary plate cover, and each sample adding auxiliary round hole is matched with a cell culture hole; according to the utility model, whether the sample adding is carried out on the sample adding auxiliary round hole or not can be quickly judged according to the fact that whether the sample adding auxiliary film corresponding to the sample adding auxiliary film round hole subjected to sample adding is torn or not, so that the manual sample adding efficiency and accuracy of the microwell plate can be improved, and the conditions of adding omission or wrong adding and the like can be prevented.

Description

Technical Field

[0001] This utility model relates to the field of manual sample addition technology for microplates, and in particular to a simple auxiliary device for manual sample addition of microplates. Background Technology

[0002] Microplates are an important tool in biomedical research. These microplates are typically made of biocompatible materials such as polymers, glass, or silicon, and are designed and manufactured to provide a platform supporting cell growth and research. For example, using microplates for culturing cells, researchers can conduct a variety of cell-related experiments and studies, including cell growth, proliferation, migration, differentiation, and apoptosis. Furthermore, these microplates can also be used to study cell-biomaterial interactions, drug screening, tissue engineering, and other applications.

[0003] Currently, microplates are a commonly used consumable in cell biology and molecular biology experiments, including 96-well cell microplates, 96-well PCR plates, and 384-well PCR plates. When manually adding samples to microplates, several factors can easily lead to missed or incorrect additions, severely impacting the accuracy of experimental results. First, the wells in microplates are numerous and closely spaced, with no clear markings between them; second, when the added sample is colorless, transparent, or in trace amounts, it is difficult for the human eye to discern whether it has been added; furthermore, visual fatigue or distraction during manual sample addition can also easily lead to errors. Therefore, developing a simple manual sample addition aid for microplates is of significant value in improving experimental efficiency and ensuring experimental quality.

[0004] Therefore, we designed a simple manual sample addition device for microplates to solve the above problems. Summary of the Invention

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a simple auxiliary device for manual sample addition to microplates.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A simple microplate manual sample loading aid includes a device body for mounting on a microplate, wherein the microplate has a plurality of cell culture wells, and a mounting plate cover is movably mounted on the microplate.

[0008] The device body includes a sample dispensing auxiliary plate cover, which is detachably installed with the microplate. The sample dispensing auxiliary plate cover is provided with several rows of equally spaced sample dispensing auxiliary holes, each of which is adapted to the cell culture well.

[0009] Each row of sample addition auxiliary holes is provided with a sample addition auxiliary film, and a sample addition auxiliary film hole is opened at the center of each row of sample addition auxiliary films;

[0010] The sample dispensing auxiliary film on the outer side of the circular holes in each row is coated with a hole position coding layer in sequence, and easy-tear lines are arranged in a circular array on the outer side of the circular holes in each row.

[0011] Preferably, the sample dispensing auxiliary plate cover is provided with rows of equally spaced sample dispensing auxiliary circular holes, and the first letter of the coding layer of the hole position coding layer coated on the rows of equally spaced sample dispensing auxiliary film is set to AH.

[0012] Preferably, each row of sample addition auxiliary circular holes is provided with 12 holes, and the last letter of the coding layer of the hole position coding layer coated on the 12 sample addition auxiliary films is set to 1-12.

[0013] Preferably, each of the sample addition auxiliary film circular holes has three easy-tear lines on its outer side.

[0014] Preferably, the length of the tear line is half the distance between the sample application auxiliary film hole and the sample application auxiliary hole.

[0015] Preferably, the device body can be made of materials such as polyvinyl chloride (PVC), polypropylene (PP) or polyethylene terephthalate (PET), which is inexpensive and disposable.

[0016] Preferably, the connection between the sample addition auxiliary film and the sample addition auxiliary circular hole can be either adhesive bonding or machine pressing.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows: In this utility model, the sample addition auxiliary plate cover and the microplate are detachably installed. The sample addition auxiliary plate cover is provided with sample addition auxiliary round holes, sample addition auxiliary film and sample addition auxiliary film round holes. The outer side of each row of sample addition auxiliary film round holes is arranged in a circular array with easy-tear lines. When adding samples to the inside of the sample addition auxiliary round holes, it is possible to quickly determine whether the sample addition auxiliary round hole has been added by observing whether the sample addition auxiliary film corresponding to the sample addition auxiliary film round hole has been torn. This helps to improve the efficiency and accuracy of manual sample addition of the microplate and prevents omissions or errors in sample addition. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of a simple manual sample addition device for microplates proposed in this utility model.

[0019] Figure 2 This is a top view of a simple microplate manual sample addition auxiliary device proposed in this utility model;

[0020] Figure 3 This is a schematic diagram of the auxiliary circular hole for adding samples in a simple microplate manual sample adding device proposed in this utility model.

[0021] In the diagram: 1. Sample addition auxiliary plate cover, 2. Sample addition auxiliary round hole, 3. Sample addition auxiliary film, 4. Sample addition auxiliary film round hole, 5. Hole location coding layer, 6. Easy-tear line. Detailed Implementation

[0022] In order to make the technical means, creative features, and achieved objectives and effects of this utility model easier to understand, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments.

[0023] Example 1

[0024] Reference Figure 1-3 A simple microplate manual sample addition auxiliary device includes a device body for mounting on the microplate. The device body is made of materials such as polyvinyl chloride (PVC), polypropylene (PP), or polyethylene terephthalate (PET). Made of such materials, it is inexpensive, disposable, and can effectively save on manufacturing costs.

[0025] In this embodiment, a number of cell culture wells are provided on the microplate, and an installation cover is movably mounted on the microplate. The device body includes a sample loading auxiliary cover 1, which is detachably mounted to the microplate. The sample loading auxiliary cover 1 is provided with a number of rows of equally spaced sample loading auxiliary round holes 2, and each sample loading auxiliary round hole 2 is adapted to the cell culture well.

[0026] More specifically, the size of the sample loading auxiliary plate cover 1 is the same as that of the mounting plate cover of the microplate, and it can be perfectly matched with the microplate; the sample loading auxiliary plate cover 1 is provided with multiple sample loading auxiliary round holes 2 that correspond one-to-one with the cell culture wells of the microplate;

[0027] In this embodiment, each row of sample addition auxiliary circular holes 2 is provided with a sample addition auxiliary film 3, and the connection between the sample addition auxiliary film 3 and the sample addition auxiliary circular holes 2 can be one of adhesive bonding or machine pressing integration. Each row of sample addition auxiliary film 3 is provided with a sample addition auxiliary film circular hole 4 at its center.

[0028] The sample-addition auxiliary film 3 on the outside of each row of sample-addition auxiliary film holes 4 is coated with hole position coding layer 5 in sequence. The hole position coding layer 5 is a coating material in the prior art. The sample-addition auxiliary plate cover 1 is provided with 8 rows of sample-addition auxiliary round holes 2 evenly distributed, and the first letter of the coding layer 5 on the 8 rows of sample-addition auxiliary film 3 is set as AH. There are 12 sample-addition auxiliary round holes 2 in each row, and the last letter of the coding layer 5 on the 12 sample-addition auxiliary film 3 is set as 1-12.

[0029] More specifically, each row of sample application auxiliary film holes 4 has three easy-tear lines 6 arranged in a circular array on the outside of each sample application auxiliary film hole 4. The length of the easy-tear line 6 is half the distance between the sample application auxiliary film hole 4 and the sample application auxiliary hole 2.

[0030] When adding samples to a microplate, first, remove the mounting plate cover and replace it with the sample addition auxiliary plate cover 1. You can identify the holes to be added based on the marked hole positions on the sample addition auxiliary film 3. Next, insert the pipette tip through the sample addition auxiliary film hole 4 on the sample addition auxiliary film 3, applying slight force to increase the insertion depth of the pipette tip. This will cause the easy-tear line 6 around the sample addition auxiliary film hole 4 to tear. Finally, after adding the sample and removing the pipette tip, you can clearly observe that the sample addition auxiliary film 3 corresponding to the sample addition auxiliary film hole 4 that has been filled with sample has been torn, and it can be quickly distinguished from the sample addition auxiliary film 3 corresponding to the sample addition auxiliary film hole 4 that has not been filled with sample.

[0031] With the above-mentioned microplate manual sample addition auxiliary device, when adding samples to the inside of the sample addition auxiliary circular hole 2, it is possible to quickly determine whether the sample addition auxiliary circular hole 2 has been added by observing whether the sample addition auxiliary film 3 corresponding to the sample addition auxiliary film circular hole 4 has been torn. This helps to improve the efficiency and accuracy of manual sample addition in microplates and prevent omissions or errors in sample addition.

[0032] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0033] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0034] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments, and various changes and modifications can be made without departing from the spirit and scope of this utility model. All such changes and modifications fall within the scope of protection claimed by this utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A simple microwell plate manual sample adding auxiliary device, comprising a device body for mounting on a microwell plate, the microwell plate being provided with a plurality of cell culture holes, and the microwell plate being movably retrofitted with a mounting plate cover, characterized in that, the device body comprises a sample adding auxiliary plate cover (1) which is detachably mounted between the sample adding auxiliary plate cover (1) and the microwell plate, the sample adding auxiliary plate cover (1) being provided with a plurality of rows of equidistantly distributed sample adding auxiliary circular holes (2), each of the sample adding auxiliary circular holes (2) being provided in correspondence with a cell culture hole; each row of the sample adding auxiliary circular holes (2) is provided with a sample adding auxiliary film (3), and the center of each row of the sample adding auxiliary film (3) is provided with a sample adding auxiliary film circular hole (4); the sample adding auxiliary film (3) outside each row of the sample adding auxiliary film circular hole (4) is sequentially coated with a hole position coding layer (5), and the outside of each row of the sample adding auxiliary film circular hole (4) is provided with a tear line (6) in a circular array distribution.

2. The simple microplate manual pipetting aid of claim 1, wherein, The sample adding auxiliary plate cover (1) is provided with 8 rows of equidistantly distributed sample adding auxiliary circular holes (2), and the coding of the hole position coding layer (5) coated on the 8 rows of equidistantly distributed sample adding auxiliary films (3) is set as A-H in the first letter.

3. The simple microplate manual pipetting aid of claim 2, wherein, Each row of the sample adding auxiliary circular holes (2) is provided with 12 sample adding auxiliary films (3), and the coding of the hole position coding layer (5) coated on the 12 sample adding auxiliary films (3) is set as 1-12 in the last letter.

4. The simple microplate manual pipetting aid of claim 1, wherein, Each of the sample adding auxiliary film circular holes (4) is provided with three tear lines (6) on the outside.

5. The simple microplate manual pipetting aid of claim 1, wherein, The length of the tear line (6) is half of the distance between the sample adding auxiliary film circular hole (4) and the sample adding auxiliary circular hole (2).

6. The simple microplate manual pipetting aid of claim 1, wherein, The device body can be made of one of polyvinyl chloride (PVC), polypropylene (PP) or polyethylene terephthalate (PET).

7. The simple microplate manual pipetting aid of claim 1, wherein, The connection between the sample adding auxiliary film (3) and the sample adding auxiliary circular hole (2) can be one of adhesive paste connection or machine-pressing integration.

Images