Rotatable adjusting type cell culture device
By introducing convex flow-guiding patterns and a spiral cell isolation mesh design into the cell culture device, combined with a cell attachment layer, the problems of cell damage and clustering caused by shear force in the rotary adjustable cell culture device were solved, and normal cell metabolism and growth were achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-06
- Publication Date
- 2026-03-13
AI Technical Summary
The shear force generated by traditional rotary adjustable cell culture devices can disrupt the adhesion between cells and the culture surface, leading to cell detachment and damage, and causing suspended cells to aggregate into cell clusters, affecting normal cell metabolism and growth.
A rotatable and adjustable cell culture device was designed, comprising an incubator, a protective cover, a rotating rack, a culture chamber, and a cell isolation mesh. Through the design of convex flow guide patterns and a spiral cell isolation mesh, combined with a cell attachment layer, the device reduces shear force damage to cells, prevents cell cluster formation, and ensures uniform distribution of the culture medium.
It effectively maintains normal cell metabolism and growth, reduces the risk of cell detachment damage, ensures good flow of culture medium between cells, and improves cell adhesion and adherent growth.
Smart Images
Figure CN223991108U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture technology, specifically to a rotatable and adjustable cell culture device. Background Technology
[0002] Cell culture is a key technology in modern biological research, enabling in-depth understanding of the physiological characteristics of cells and the mechanisms of disease. Therefore, in the research process, researchers need to accurately simulate the microenvironment of cells in vivo in order to explore cell functions.
[0003] Traditional cell culture devices, such as simple culture flasks and dishes, can only provide a static culture environment for cells, which is difficult to meet the complex dynamic environment requirements that cells face in vivo. Components such as serum, calcium and magnesium ions in the culture medium are prone to uneven distribution, resulting in differences in nutrient acquisition by cells and affecting the consistency of cell growth.
[0004] Therefore, rotary adjustable cell culture devices are used to simulate complex physiological environments, providing dynamic culture conditions for cell growth and achieving uniform mixing of the culture medium. However, the shear force generated by the operation of the rotary adjustable cell culture device can disrupt the adhesion between cells and the culture surface, leading to cell detachment and damage, which in turn affects cell adherence and growth. Excessive shear force can also cause suspended cells to aggregate and form cell clusters, affecting normal cell metabolism and growth.
[0005] Therefore, this invention proposes a rotatable adjustable cell culture device to solve the above-mentioned problems. Utility Model Content
[0006] The purpose of this invention is to provide a rotatable and adjustable cell culture device to solve the problem in the prior art that the shear force generated by rotational adjustment will destroy the adhesion between cells and the culture surface, and will also cause suspended cells to aggregate and form cell clusters, affecting the normal metabolism and growth of cells.
[0007] The technical solution adopted by this utility model to solve its technical problem is:
[0008] A rotatable and adjustable cell culture device includes an incubator with a protective cover slidably connected to its top. A support base and a drive motor are disposed inside the incubator. A rotating frame is connected to the support base via bearings, and a synchronous pulley is connected to the rotating frame. The drive motor and the synchronous pulley are connected via a synchronous belt. A culture chamber is clamped and connected to the rotating frame. The culture chamber includes a culture cavity and sealed end caps connected to both ends of the culture cavity. An inlet and an outlet are respectively connected to the sealed end caps. The culture cavity is transparent. The inlet and outlet are respectively connected to a rotary joint via pipes. The inner wall of the culture cavity has convex flow-guiding patterns. A cell isolation mesh is connected inside the culture cavity, and the cell isolation mesh is spirally arranged inside the culture cavity. Each sealed end cap has a cell adhesion layer, which is detachably connected to the sealed end cap.
[0009] Furthermore, the rotary joint includes a fixed part and a rotating part, the fixed part being connected to the side wall of the incubator, and the rotating part being connected to the pipe.
[0010] Furthermore, the liquid inlet is provided with a buffer chamber, an elastic diaphragm, and multiple flow equalization plates.
[0011] Furthermore, the rotating frame includes two parallel frame plates, each frame plate having a slot with an elastic buffer pad inside. A locking block is connected to the culture chamber corresponding to the slot, and a fixing plate is connected to the frame plate corresponding to the culture chamber. The fixing plate is snapped into the frame plate.
[0012] Furthermore, a sealing strip is provided on the top of the incubator corresponding to the protective cover, and an observation window is provided on the protective cover. The observation window is made of transparent material, and a light shield is slidably connected to the observation window.
[0013] Furthermore, a heating wire is provided at the bottom of the incubator, a cooling fan is connected to the side wall of the incubator, and a temperature sensor is connected inside the incubator.
[0014] In summary, compared with the prior art, the beneficial effects of this utility model are as follows:
[0015] The convex flow-guiding patterns on the inner wall of the culture chamber of this invention guide the culture medium to form a specific flow path when the culture chamber rotates, allowing cells to fully contact nutrients. The cell isolation mesh is spirally arranged, which not only prevents cells from aggregating and forming cell clusters in the culture chamber, but also ensures good flow of the culture medium between cells, thereby maintaining normal cell metabolism and growth. The cell attachment layer provides initial attachment sites for cells, enhances cell adhesion, reduces the risk of cell detachment and damage caused by the shear force of the device operation, and ensures adherent cell growth. Attached Figure Description
[0016] Figure 1 This is a schematic diagram of the usage state 1 of this utility model;
[0017] Figure 2 This is a schematic diagram of the second usage state of this utility model;
[0018] Figure 3 for Figure 2 Top view;
[0019] Figure 4 for Figure 2 A partial sectional view of the front view;
[0020] In the diagram: 1. Incubator; 2. Protective cover; 3. Sealing strip; 4. Observation window; 5. Light shield; 6. Support base; 7. Drive motor; 8. Bearing; 9. Rotating frame; 10. Synchronous pulley; 11. Synchronous belt; 12. Culture chamber; 13. Sealed end cap; 14. Liquid inlet; 15. Buffer chamber; 16. Elastic diaphragm; 17. Flow equalization plate; 18. Liquid outlet; 19. Pipe; 20. Convex guide groove; 21. Cell isolation mesh; 22. Cell attachment layer; 23. Fixing part; 24. Rotating part; 25. Frame plate; 26. Slot; 27. Elastic buffer pad; 28. Locking block; 29. Fixing plate; 30. Heating wire; 31. Cooling fan; 32. Temperature sensor. Detailed Implementation
[0021] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.
[0022] In this application, the terms "upper," "inner," "outer," "middle," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing this application and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.
[0023] like Figure 1-4The described rotatable and adjustable cell culture device includes an incubator 1. A protective cover 2 is slidably connected to the top of the incubator 1. A sealing strip 3 is provided on the top of the incubator 1 corresponding to the protective cover 2. An observation window 4 is provided on the protective cover 2. The observation window 4 is made of transparent material, and a light-shielding plate 5 is slidably connected to the observation window 4. A heating wire 30 is provided at the bottom of the incubator 1. A cooling fan 31 is connected to the side wall of the incubator 1. A temperature sensor 32 is connected inside the incubator 1.
[0024] The incubator 1 is equipped with a support base 6 and a drive motor 7. A rotating frame 9 is connected to the support base 6 via a bearing 8. A synchronous pulley 10 is connected to the rotating frame 9. The drive motor 7 and the synchronous pulley 10 are connected via a synchronous belt 11. A culture chamber is clamped and connected to the rotating frame 9. The rotating frame 9 includes two parallel frame plates 25. A slot 26 is opened on the frame plate 25. An elastic buffer pad 27 is provided in the slot 26. A locking block 28 is connected to the culture chamber 12 corresponding to the slot 26. A fixing plate 29 is connected to the frame plate 25 corresponding to the culture chamber. The fixing plate 29 is snapped and connected to the frame plate 25. The culture chamber includes a culture chamber 12 and a sealing end cap 13 connected to both ends of the culture chamber 12. The sealing end cap 13 is connected to an inlet port 14 and an outlet port 18 respectively. The culture chamber 12 is transparent. The inlet port 14 and the outlet port 18 are connected to a rotary joint through pipes 19 respectively. The inlet port 14 is provided with a buffer chamber 15, an elastic diaphragm 16 and multiple flow equalization plates 17.
[0025] The inner wall of the culture chamber 12 is provided with convex flow-guiding patterns 20. A cell isolation net 21 is connected inside the culture chamber 12. The cell isolation net 21 is arranged in a spiral shape inside the culture chamber 12. Each sealing end cap 13 is provided with a cell adhesion layer 22, which is detachably connected to the sealing end cap 13. The rotary joint includes a fixed part 23 and a rotating part 24. The fixed part 23 is connected to the side wall of the incubator 1, and the rotating part 24 is connected to the pipe 19.
[0026] The working process of this utility model is as follows:
[0027] First, the cell suspension is injected into the culture chamber 12 of the culture chamber, and then the culture chamber 12 is sealed by the sealing end cap 13. Then, the cell attachment layer 22 provides the initial attachment site for the cells, which drives the motor 7 to start running. The power of the motor is transmitted to the synchronous pulley 10 through the synchronous belt 11, which drives the rotating frame 9 to rotate around the bearing 8, and the culture chamber 12 rotates synchronously.
[0028] During the rotation of the culture chamber, the external culture medium flows into the culture chamber 12 through the inlet port 14 via the pipe 19. The buffer chamber 15 inside the inlet port 14 can slow down the liquid flow rate to avoid excessive impact on the cells. The elastic diaphragm 16 can regulate the internal pressure to maintain a stable liquid environment. Multiple flow equalization plates 17 can ensure that the culture medium enters the culture chamber 12 evenly. The outlet port 18 can discharge the culture medium containing metabolic waste from the culture chamber 12, so that the culture medium circulates and thus maintains the stability of the cell culture environment.
[0029] The convex flow-guiding grooves 20 on the inner wall of the culture chamber 12 guide the culture medium to form a specific flow path when the culture chamber rotates, allowing cells to fully contact nutrients. The cell isolation mesh 21 is spirally arranged, preventing cells from aggregating and forming cell clusters within the culture chamber 12 while ensuring good flow of the culture medium between cells. As the culture progresses, cells continuously grow and proliferate within the culture chamber 12. Researchers can monitor the cell growth status in real time through the observation window 4. If light affects cell growth, the light-blocking plate 5 can be slid to block the light.
[0030] The heating wire 30 at the bottom of the incubator 1 is used to maintain a suitable temperature inside the incubator and provide a suitable thermal environment for cell growth. The temperature sensor 32 monitors the temperature inside the incubator in real time and feeds the data back to the control device, thereby precisely controlling the working state of the heating wire 30. When the temperature inside the incubator 1 is too high, the cooling fan 31 on the side wall is activated to accelerate air circulation, reduce the temperature, and ensure that the cells are always within the optimal culture temperature range.
Claims
1. A rotatable adjustable cell culture device comprising a culture chamber (1), characterized in that, The top of the incubator (1) is slidably connected with a protective cover (2), the incubator (1) is provided with a support seat (6) and a drive motor (7), the support seat (6) is connected with a rotating frame (9) through a bearing (8), the rotating frame (9) is connected with a synchronous wheel (10), the drive motor (7) is connected with the synchronous wheel (10) through a synchronous belt (11); the rotating frame (9) is clamped with a culture chamber, the culture chamber includes a culture cavity (12) and a sealing end cover (13) connected at both ends of the culture cavity (12), the sealing end cover (13) is connected with a liquid inlet interface (14) and a liquid outlet interface (18) respectively, the culture cavity (12) is transparent, the liquid inlet interface (14) and the liquid outlet interface (18) are connected with a rotating joint through a pipeline (19) respectively; The inner wall of the culture cavity (12) is provided with a convex flow guide pattern (20), the culture cavity (12) is connected with a cell isolation net (21), the cell isolation net (21) is spirally arranged in the culture cavity (12), the sealing end cover (13) is provided with a cell adhesion layer (22), and the cell adhesion layer (22) is detachably connected with the sealing end cover (13).
2. The rotatable adjustable cell culture device of claim 1, wherein, The rotating joint includes a fixed part (23) and a rotating part (24), the fixed part (23) is connected to the side wall of the incubator (1), and the rotating part (24) is connected with the pipeline (19).
3. The rotatable adjustable cell culture device of claim 1, wherein, The liquid inlet interface (14) is provided with a buffer chamber (15), an elastic diaphragm (16) and a plurality of flow equalizing hole plates (17).
4. The rotatable adjustable cell culture device of claim 1, wherein, The rotating frame (9) includes two parallel frame plates (25), the frame plate (25) is provided with a clamping groove (26), the culture cavity (12) is connected with a clamping block (28) corresponding to the clamping groove (26), and the frame plate (25) is connected with a fixed plate (29) corresponding to the culture chamber.
5. The rotatable adjustable cell culture device of claim 4, wherein, The clamping groove (26) is provided with an elastic buffer pad (27).
6. The rotatable adjustable cell culture device of claim 1, wherein, The top of the incubator (1) is provided with a sealing strip (3) corresponding to the protective cover (2), the protective cover (2) is provided with an observation window (4), the observation window (4) is made of transparent material, and the observation window (4) is slidably connected with a light shield (5).
7. The rotatable adjustable cell culture device of claim 1, wherein, The bottom of the incubator (1) is provided with a heating wire (30), the side wall of the incubator (1) is connected with a cooling fan (31), and the inside of the incubator (1) is connected with a temperature sensor (32).