Ultrafiltration membrane purification and separation device for protein

Abstract

The ultrafiltration membrane purification and separation device comprises a support, a discharging pipe and a connecting rod, mounting grooves are symmetrically and fixedly connected to the two sides of the support, ultrafiltration membrane assemblies are mounted in the mounting grooves, the multiple ultrafiltration membrane assemblies on one side of the support are connected in series, and the discharging pipe is connected with the connecting rod. According to the utility model, a plurality of ultrafiltration membrane components on one side of the bracket are connected in series, and the aperture of each stage of ultrafiltration membrane wire is sequentially reduced from the feeding pipe. Due to the design, a mixed solution containing target protein can be gradually and finely separated according to the molecular size when passing through each stage of ultrafiltration membrane assembly. Firstly, large impurities are preliminarily intercepted by the ultrafiltration membrane filaments with large apertures, and small molecular impurities are continuously removed as a solution sequentially passes through the ultrafiltration membrane filaments with gradually reduced apertures, so that deep purification of proteins is realized, the purity of target proteins is effectively improved, and the requirements of the fields of biochemistry, biomedicine and the like on high-purity proteins are met.

Description

Technical Field

[0001] This utility model relates to the field of protein filtration technology, specifically to an ultrafiltration membrane purification and separation device for proteins. Background Technology

[0002] In numerous fields such as biochemistry, biomedicine, and biotechnology, protein purification and separation are crucial steps. As macromolecules that perform various vital physiological functions within organisms, the purity and activity of proteins play a decisive role in related research and product development. For example, in drug development, high-purity protein drugs can improve therapeutic efficacy and reduce the risk of side effects; in basic research, pure protein samples help accurately elucidate the structure-function relationship of proteins.

[0003] There are many traditional methods for protein purification and separation, such as precipitation and chromatography. While precipitation is relatively simple to operate, it often fails to accurately remove impurities, easily leading to the loss of the target protein, and subsequent processing is quite cumbersome. Although chromatography provides better separation results, the equipment is complex and expensive, requires highly skilled operators, and has limited throughput, making it difficult to meet the needs of large-scale production.

[0004] With technological advancements, ultrafiltration membrane technology has been increasingly applied to protein separation. Ultrafiltration membranes can separate mixtures based on molecular size, offering advantages such as no phase change, ease of operation, and minimal damage to bioactive substances. However, existing ultrafiltration membrane devices still present several challenges in practical applications. Firstly, most ultrafiltration membrane devices employ a single-structure ultrafiltration membrane module, making it difficult to flexibly adjust filtration precision according to the characteristics of different proteins. This hinders multi-stage fine separation of protein solutions, limiting the improvement of protein purity. Secondly, replacing ultrafiltration membrane modules is extremely inconvenient. Traditional devices often require complex disassembly procedures and lack effective auxiliary tools. This not only consumes significant manpower and time but also easily damages other components during operation, affecting the overall lifespan and operational stability of the device.

[0005] Therefore, we propose an ultrafiltration membrane purification and separation device for proteins. Utility Model Content

[0006] The purpose of this invention is to provide an ultrafiltration membrane purification and separation device for proteins to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, this utility model provides the following technical solution: A protein ultrafiltration membrane purification and separation device includes: a support, a discharge pipe, and a connecting rod. The support has symmetrically fixed mounting grooves on both sides, each groove containing an ultrafiltration membrane assembly. Several ultrafiltration membrane assemblies on one side of the support are connected in series, and the bottom end of the ultrafiltration membrane assembly at the end of the support is connected to a feed pipe. Discharge pipes are symmetrically installed on both sides of the top of the support, with two discharge pipes connected to the top ends of two adjacent ultrafiltration membrane assemblies. A discharge pipe is installed at the bottom of the support, connected to the bottom ends of two adjacent ultrafiltration membrane assemblies. The two discharge pipes are connected to the discharge pipe. A connecting rod is provided inside the support, with a top rod symmetrically and rotatably mounted at the top of the connecting rod. A lifting rope is installed inside the top rod. A driving structure is installed between the support and the connecting rod.

[0008] Preferably, the ultrafiltration membrane assembly includes a housing, ultrafiltration membrane fibers installed inside the housing, and epoxy end caps installed at both ends of the housing.

[0009] Preferably, the pore size of each ultrafiltration membrane fiber in the ultrafiltration membrane assembly decreases sequentially from the feed tube.

[0010] Preferably, the driving structure includes: a threaded rod, a threaded slider, a support rod, and a crossbeam. The threaded rod is rotatably connected inside the bracket, and the two ends of the threaded rod are symmetrically threaded to the threaded sliders. The two threaded sliders are slidably connected to the inside of the bracket, and the top ends of the two threaded sliders are rotatably connected to the support rods. The ends of the two support rods away from the threaded sliders are rotatably connected to the crossbeam, and the connecting rod is slidably connected to the top of the crossbeam. The threaded rod is a bidirectional threaded rod.

[0011] Preferably, a connecting piece is fixedly connected to the top end of the connecting rod, and a spring is fixedly connected inside the connecting piece, with both ends of the spring in contact with the sides of the two top rods that are close to each other.

[0012] Preferably, an elastic rope is fixedly connected inside the top rod, and a hook is fixedly connected to one end of the elastic rope, which hooks the lifting rope.

[0013] Preferably, one side of the bracket is fitted with a clamp by bolts at the mounting groove.

[0014] Preferably, a tee is provided at the connection points of the discharge pipe and the feed pipe with the ultrafiltration membrane module.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] Highly efficient and precise protein purification and separation effect

[0017] Multi-stage tandem ultrafiltration design: Several ultrafiltration membrane modules on one side of the support are connected in series, with the pore size of each ultrafiltration membrane fiber decreasing sequentially from the feed tube. This design allows for progressive and precise separation of the target protein mixture as it passes through each stage of the ultrafiltration membrane module, based on molecular size. Initially, the larger pore size ultrafiltration membrane fibers trap larger impurities. As the solution passes through the gradually decreasing pore size ultrafiltration membrane fibers, smaller molecule impurities are continuously removed, thereby achieving deep purification of the protein. This effectively improves the purity of the target protein and meets the demand for high-purity proteins in fields such as biochemistry and biomedicine.

[0018] Multi-channel discharge design: Symmetrically installed discharge pipes on both sides of the top of the support are used to discharge the final permeate, while the discharge pipe at the bottom is used to discharge the concentrated protein solution. T-junctions at the connection points of the discharge and discharge pipes to the ultrafiltration membrane module ensure that the liquid can smoothly and accurately enter the corresponding discharge pipe from the ultrafiltration membrane module, achieving effective separation of permeate and retentate and improving separation efficiency.

[0019] Convenient ultrafiltration membrane module replacement operation

[0020] Adjustable lifting structure: The device is equipped with a drive structure. By rotating the bidirectional threaded rod, the two threaded sliders move closer or further apart, thereby driving the support rod and crossbeam to rise and fall, thus achieving the raising and lowering of the connecting rod. When it is necessary to replace the ultrafiltration membrane module, the connecting rod can be removed from the support. This adjustable lifting structure provides operators with a convenient operating height, reduces the difficulty of manual handling and disassembly, and lowers labor intensity.

[0021] The automatically deployable top rod structure: Under the action of a spring, the top rod at the top of the connecting rod automatically deploys to form a T-shape with the connecting rod when the connecting rod is removed from the support. The elastic rope and hook inside the top rod can easily hook onto the lifting rope for securing the ultrafiltration membrane module that needs to be replaced or disassembled. This design makes the hoisting and disassembly of the ultrafiltration membrane module more convenient and faster, improving the efficiency of replacing the ultrafiltration membrane module while reducing the risk of damage to other components of the device during the replacement process. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0023] Figure 2 This is a partial cross-sectional view of the support structure of this utility model;

[0024] Figure 3 This is a schematic diagram of the suspension rope structure of this utility model.

[0025] In the diagram: 1. Support; 2. Mounting groove; 3. Ultrafiltration membrane module; 4. Feed pipe; 5. Discharge pipe; 6. Discharge pipe; 7. Connecting rod; 8. Top rod; 9. Lifting rope; 10. Drive structure; 11. Spring; 12. Clamp; 21. Threaded rod; 22. Threaded slider; 23. Support rod; 24. Crossbeam. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Please see Figure 1-3 As shown, an ultrafiltration membrane purification and separation device for proteins includes a support 1, a discharge pipe 5, and a connecting rod 7. The support 1 has symmetrically fixed mounting grooves 2 on both sides, each containing an ultrafiltration membrane assembly 3. Several ultrafiltration membrane assemblies 3 on one side of the support 1 are connected in series, and the bottom of the ultrafiltration membrane assembly 3 located at the end of the support 1 is connected to a feed pipe 4. Discharge pipes 6 are symmetrically installed on both sides of the top of the support 1, with two discharge pipes 6 connected to the tops of two adjacent ultrafiltration membrane assemblies 3. A discharge pipe 5 is installed at the bottom of the support 1, also connected to the bottom of two adjacent ultrafiltration membrane assemblies 3. The support 1 is connected to the discharge pipe 5. A connecting rod 7 is provided inside the support 1. A top rod 8 is symmetrically and rotatably installed at the top of the connecting rod 7. A lifting rope 9 is installed inside the top rod 8. A driving structure 10 is installed between the support 1 and the connecting rod 7. The ultrafiltration membrane module 3 includes a shell, ultrafiltration membrane fibers installed inside the shell, and epoxy end caps installed at both ends of the shell. The pore size of each stage of ultrafiltration membrane fibers in the ultrafiltration membrane module 3 decreases sequentially from the feed pipe 4. A clamp 12 is installed on one side of the support 1 at the mounting groove 2 by bolts. A tee is provided at the connection points of the discharge pipe 6 and the discharge pipe 5 with the ultrafiltration membrane module 3.

[0028] Please see Figure 2-3 As shown, the driving structure 10 includes: a threaded rod 21, a threaded slider 22, a support rod 23, and a crossbeam 24. The threaded rod 21 is rotatably connected inside the bracket 1. The two ends of the threaded rod 21 are symmetrically threaded to the threaded sliders 22. The two threaded sliders 22 are slidably connected to the inside of the bracket 1. The top ends of the two threaded sliders 22 are rotatably connected to the support rods 23. The ends of the two support rods 23 away from the threaded sliders 22 are rotatably connected to the crossbeam 24. The connecting rod 7 is slidably connected to the top end of the crossbeam 24. The threaded rod 21 is a bidirectional threaded rod.

[0029] Please see Figure 3As shown, a connecting piece is fixedly connected to the top of the connecting rod 7, and a spring 11 is fixedly connected inside the connecting piece. The two ends of the spring 11 are in contact with the two top rods 8 on the side that are close to each other. An elastic rope is fixedly connected inside the top rod 8, and a hook is fixedly connected to one end of the elastic rope. The hook hooks the lifting rope 9.

[0030] Working principle:

[0031] Ultrafiltration process

[0032] Feed: The mixed solution containing the target protein enters the ultrafiltration membrane assembly 3 located at the end of the support 1 through the feed pipe 4; Since the pore size of each ultrafiltration membrane fiber in the ultrafiltration membrane assembly 3 decreases sequentially from the feed pipe 4, the mixed solution first contacts the ultrafiltration membrane fiber with a relatively larger pore size.

[0033] Step-by-step filtration: Under pressure, small molecules such as water, salt, and small metabolites in the mixed solution can pass through the ultrafiltration membrane fibers, while large protein molecules are retained. The solution that has passed through the previous ultrafiltration membrane fiber will enter the next stage of the series-connected ultrafiltration membrane module 3 to continue to be filtered. As the solution passes through each stage of the ultrafiltration membrane module in sequence, small molecule impurities in the solution are continuously removed, and proteins are gradually purified and concentrated.

[0034] Discharge: The final permeate after filtration by each stage of ultrafiltration membrane module 3 flows out from the discharge pipes 6 on both sides of the top of the support 1. At the same time, the concentrated protein solution retained by each stage of ultrafiltration membrane module 3 is discharged from the discharge pipe 5 at the bottom of the support 1. The tee valves set at the connection points of the discharge pipe 6 and the discharge pipe 5 with the ultrafiltration membrane module 3 ensure that the liquid can smoothly and accurately enter the corresponding discharge pipe from the ultrafiltration membrane module.

[0035] Ultrafiltration membrane module replacement process

[0036] Drive structure start-up: When it is necessary to replace the ultrafiltration membrane module, the threaded rod 21 in the drive structure 10 is rotated by an external tool; since the threaded rod 21 is a bidirectional threaded rod, during the rotation, the threaded sliders 22 that are symmetrically threaded at both ends will move closer to each other; the threaded sliders 22 slide inside the support 1, and the support rod 23 that is rotatably connected at its top end changes its angle as the threaded sliders 22 move.

[0037] Actions of connecting rod and top rod: The top of the support rod 23 drives the crossbeam 24 to rise, which in turn pushes the connecting rod 7 to rise; when the connecting rod 7 moves out of the bracket 1, the spring 11 fixed inside the connecting piece at the top of the connecting rod 7 will push the two top rods 8 to unfold each other and form a T-shaped structure with the connecting rod 7;

[0038] Ultrafiltration membrane module disassembly: Hook the hook fixed to one end of the elastic rope inside the top rod 8 onto the lifting rope 9, and tie the lifting rope 9 to the ultrafiltration membrane module to be replaced or disassembled; at this time, by continuing to rotate the threaded rod 21 to raise and lower the connecting rod 7, the ultrafiltration membrane module can be raised and lowered, which is convenient for workers to disassemble or install; after the new ultrafiltration membrane module is installed, reverse the operation of the drive structure 10 to return the connecting rod 7 and the top rod 8 to their original positions, completing the replacement of the ultrafiltration membrane module; the clamp 12 on one side of the bracket 1, which is installed in the mounting groove 2 by bolts, can be used to further stabilize the position of the ultrafiltration membrane module in the mounting groove 2 after the replacement of the ultrafiltration membrane module.

[0039] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0040] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An apparatus for the purification and isolation of a protein by ultrafiltration membrane, characterized in that, Include: Support (1), both sides of support (1) symmetrical fixed connection has installation groove (2), installation groove (2) inside all install ultrafiltration membrane assembly (3), several ultrafiltration membrane assembly (3) of support (1) one side is connected in series, and the ultrafiltration membrane assembly (3) bottom end communication of the end of support (1) has feed pipe (4); Discharge pipe (5), support (1) top end both sides symmetrical installation has discharge pipe (6), two discharge pipe (6) and two adjacent ultrafiltration membrane assembly (3) top end communication, support (1) bottom end installation has discharge pipe (5), discharge pipe (5) and two adjacent ultrafiltration membrane assembly (3) bottom end communication, two discharge pipe (6) and discharge pipe (5) communication; Connecting rod (7), support (1) inside be provided with connecting rod (7), connecting rod (7) top end symmetrical rotation installation has top rod (8), top rod (8) inside installation has sling (9), support (1) and connecting rod (7) between installation has drive structure (10).

2. The apparatus for purifying and separating a protein by ultrafiltration according to claim 1, wherein: The ultrafiltration membrane assembly (3) comprises a shell, an ultrafiltration membrane wire installed in the shell and an epoxy end cap installed at both ends of the shell.

3. The apparatus for purifying and separating a protein by ultrafiltration according to claim 2, wherein: The ultrafiltration membrane assembly (3) from the feed pipe (4), the pore size of each ultrafiltration membrane wire decreases in turn.

4. The apparatus for purifying and separating a protein by using an ultrafiltration membrane according to claim 1, wherein: The drive structure (10) comprises: a threaded rod (21), a threaded slide block (22), a brace (23) and a cross beam (24), the inside of the support (1) is rotatably connected with the threaded rod (21), the both ends of the threaded rod (21) are symmetrically connected with the threaded slide block (22), the two threaded slide blocks (22) are respectively connected with the inside of the support (1) through sliding, the top ends of the two threaded slide blocks (22) are rotatably connected with the brace (23), the ends of the two braces (23) away from the threaded slide block (22) are rotatably connected with the cross beam (24), the connecting rod (7) is connected with the top end of the cross beam (24) through sliding, and the threaded rod (21) is a bidirectional threaded rod.

5. The apparatus for purifying and separating a protein by ultrafiltration according to claim 1, wherein: The top end of the connecting rod (7) is fixedly connected with a connecting piece, the inside of the connecting piece is fixedly connected with a spring (11), and the both ends of the spring (11) are in contact with one side of the two top rods (8) close to each other.

6. The apparatus for purifying and separating a protein by using an ultrafiltration membrane according to claim 1, wherein: The inside of the top rod (8) is fixedly connected with an elastic rope, one end of the elastic rope is fixedly connected with a hook, and the hook hooks the sling (9).

7. The apparatus for purifying and separating a protein by using an ultrafiltration membrane according to claim 1, wherein: One side of the support (1) is provided with a hoop (12) at the installation groove (2) through bolts.

8. The apparatus for purifying and separating a protein by using an ultrafiltration membrane according to claim 1, wherein: The discharge pipe (6) and the discharge pipe (5) are respectively provided with a tee joint at the connection with the ultrafiltration membrane assembly (3).

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