Fluorescent staining kit device for fungus D-glucan detection

Abstract

The utility model belongs to the technical field of kits, and particularly relates to a fungal D-glucan detection fluorescent staining kit device which comprises a kit body, the top of the box body is rotationally connected with a first cover plate; the middle part of the box body is fixedly connected with a bearing plate; a plurality of placement holes are formed in the middle of the bearing plate; a plurality of bearing cylinders are fixedly connected to the bottom of the bearing plate; a plurality of clamping plates are slidably connected to the middle of the bearing cylinder. An elastic film sleeve is fixedly connected to the middle parts of the plurality of clamping plates; a plurality of through holes are formed in the middle of the bearing cylinder; a placement groove is formed in the side wall of the box body; the top of the box body is rotationally connected with a second cover plate; a plurality of first through grooves are formed in the side wall of the placement groove; by means of the structure, after the fungal D-glucan detection fluorescent staining reagent bottle is put into the box, the reagent bottle can be extruded and fixed, the situation that the reagent bottle is loosened and shaken due to the small diameter is reduced, and the reagent bottle is more stable in the box.

Description

Technical Field

[0001] This invention belongs to the field of reagent kit technology, specifically a fluorescent staining reagent kit device for detecting fungal D-glucan. Background Technology

[0002] The fungal D-glucan detection fluorescent staining reagent is a laboratory reagent used to detect fungal infections. Based on fluorescent staining technology, it specifically binds to D-glucan components in the fungal cell wall, generating observable fluorescent signals under a fluorescence microscope, thereby enabling the detection of fungi.

[0003] Fluorescent staining reagents for fungal D-glucan detection are usually packaged in reagent bottles. Multiple reagent bottles are then loaded into the kit to facilitate transfer between them. To prevent interference between the reagent bottles after loading, existing kits often have independent placement slots for each bottle. However, the size of the placement slots is difficult to change, while the diameters of the reagent bottles vary. When a thinner bottle is placed in a larger placement slot, it will wobble when the kit is moved, resulting in poor stability.

[0004] Therefore, this invention provides a fluorescent staining kit for detecting fungal D-glucan. Utility Model Content

[0005] In order to overcome the shortcomings of the prior art, at least one technical problem raised in the background art is solved.

[0006] The technical solution adopted by this utility model to solve its technical problem is as follows: The device for a fluorescent staining reagent kit for detecting fungal D-glucan includes a box body; a first cover plate is rotatably connected to the top of the box body; a support plate is fixedly connected to the middle of the box body; multiple placement holes are opened in the middle of the support plate; multiple support cylinders are fixedly connected to the bottom of the support plate; multiple clamping plates are slidably connected to the middle of the support cylinders; and elastic membrane sleeves are fixedly connected to the middle of the multiple clamping plates. Through the above structure, after the fluorescent staining reagent bottle for detecting fungal D-glucan is put into the box, the reagent bottle can be squeezed and fixed, reducing the occurrence of loosening and shaking of the reagent bottle due to its small diameter, and making the reagent bottle more stable in the box.

[0007] Preferably, the carrier cylinder has multiple through holes in the middle; the side wall of the box body has a placement groove; the top of the box body is rotatably connected to a second cover plate; the side wall of the placement groove has multiple first through grooves; with the above structure, the temperature inside the box body can be controlled when transferring reagents, reducing the impact of excessively low or high temperatures on the activity of the reagents.

[0008] Preferably, the top of the support plate is rotatably connected to multiple baffles via torsion springs; the top of the box body is fixedly connected to multiple limiting blocks; and the bottom inner side of the support cylinder is fixedly connected to a spring. With the above structure, after the reagent bottle is placed inside the box body, the shaking of the reagent bottle can be further reduced, the stability of the reagent bottle inside the box body can be improved, and when the reagent bottle needs to be used, the reagent bottle can be quickly removed from the support cylinder, improving the ease of use of the device.

[0009] Preferably, a top plate is fixed to the top of the spring; multiple sleeves are fixed to the bottom of the box; the sleeves are located outside the support cylinder and surround the portions of the multiple clamping plates located outside the support cylinder; with the above structure, the influence of outside air on the air temperature below the support plate can be reduced when the reagent bottle is taken out, thereby reducing the impact on the overall preservation of the reagent bottle.

[0010] Preferably, a partition is slidably connected to the side wall of the placement slot; a plurality of second through slots are opened in the middle of the partition; an elastic rope is fixedly connected to the side wall of the partition; the top end of the elastic rope is fixedly connected to the middle of the side wall of the placement slot; with the above structure, when the temperature regulating medium inside the placement slot is replaced, the influence of the support plate on the air temperature below can be reduced, thereby reducing the influence on the storage temperature of the reagent bottle.

[0011] Preferably, multiple elastic bands are fixedly connected to the inside of the box; a counterweight is fixedly connected to the middle of each elastic band; through the above structure, the air temperature inside the box can be more uniform when the reagent bottle is transferred, thereby improving the control effect of the reagent bottle storage temperature.

[0012] Preferably, a sealing gasket is fixed to the top of the top plate; the sealing gasket is made of a rubber material; through the above structure, after a reagent bottle is removed from the carrier cylinder, the top of the top plate can make the contact between the top of the top plate and the middle of the carrier cylinder more compact, further reducing the flow between the outside air and the air below the carrier plate, and reducing heat loss.

[0013] The beneficial effects of this utility model are as follows:

[0014] 1. The device for a fluorescent staining reagent kit for detecting fungal D-glucan, as described in this utility model, through the arrangement of a box body, a first cover plate, a support plate, a placement hole, a support cylinder, a clamping plate, and an elastic membrane sleeve, can compress and fix the reagent bottle after it is placed in the box, reducing the occurrence of loosening and shaking of the reagent bottle due to its small diameter, and making the reagent bottle more stable in the box.

[0015] 2. The fluorescent staining reagent kit for detecting fungal D-glucan, as described in this utility model, can control the internal temperature of the kit during reagent transfer by setting up through holes, placement grooves, a second cover plate, and a first through groove, thereby reducing the impact of excessively low or high temperatures on reagent activity. Attached Figure Description

[0016] The present invention will be further described below with reference to the accompanying drawings.

[0017] Figure 1 This is a perspective view of the present invention;

[0018] Figure 2 This is a schematic diagram of the structure of the bearing cylinder in this utility model;

[0019] Figure 3 This is a schematic diagram of the structure of the clamping plate in this utility model;

[0020] Figure 4 This is a schematic diagram of the placement groove in this utility model.

[0021] In the diagram: 1. Box body; 12. First cover plate; 13. Support plate; 14. Placement hole; 15. Support cylinder; 16. Clamping plate; 17. Elastic membrane sleeve; 2. Through hole; 21. Placement groove; 22. Second cover plate; 23. First through groove; 3. Baffle; 31. Limiting block; 32. Spring; 4. Top plate; 41. Shell; 5. Partition plate; 51. Second through groove; 52. Elastic rope; 6. Elastic band; 61. Counterweight; 7. Sealing gasket. Detailed Implementation

[0022] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0023] like Figures 1 to 3As shown in the figure, an embodiment of the present invention provides a fluorescent staining reagent kit for detecting fungal D-glucan, comprising a box body 1; a first cover plate 12 is rotatably connected to the top of the box body 1; a support plate 13 is fixedly connected to the middle of the box body 1; a plurality of placement holes 14 are opened in the middle of the support plate 13; a plurality of support cylinders 15 are fixedly connected to the bottom of the support plate 13; a plurality of clamping plates 16 are slidably connected to the middle of the support cylinders 15; an elastic membrane sleeve 17 is fixedly connected to the middle of the plurality of clamping plates 16; during operation, the reagent bottle is inserted into the support cylinder 15 through the placement hole 14. After the reagent bottle enters the support cylinder 15, the plurality of clamping plates 16 will approach each other under the elastic force of the elastic membrane sleeve 17 and squeeze the reagent bottle, making it difficult for the thinner reagent bottle to shake inside the support cylinder 15. Through the above structure, after the fluorescent staining reagent bottle for detecting fungal D-glucan is put into the box, the reagent bottle can be squeezed and fixed, reducing the occurrence of loosening and shaking of the reagent bottle due to its small diameter, and making the reagent bottle more stable in the box.

[0024] like Figures 1 to 4 As shown, the carrier cylinder 15 has multiple through holes 2 in the middle; the side wall of the box body 1 has a placement groove 21; the top of the box body 1 is rotatably connected to a second cover plate 22; the side wall of the placement groove 21 has multiple first through grooves 23; during operation, after the reagent bottle is placed inside the box body 1, a temperature regulating medium can be added to the placement groove 21, and then the placement groove 21 is closed by the second cover plate 22, so that the medium exchanges heat with the air inside the placement groove 21 and the box body 1, so that the temperature of the air inside the box body 1 gradually changes to a temperature suitable for reagent preservation. The air after the temperature change will enter the carrier cylinder 15 through the through holes 2 and exchange heat with the reagent bottle, thereby reducing the impact of temperature on reagent activity when transferring reagents. Through the above structure, the temperature inside the box body 1 can be controlled when transferring reagents, reducing the impact of excessively low or high temperatures on reagent activity.

[0025] like Figures 1 to 3As shown, multiple baffles 3 are rotatably connected to the top of the support plate 13 via torsion springs; multiple limiting blocks 31 are fixedly connected to the top of the box body 1; a spring 32 is fixedly connected to the bottom inner side of the support cylinder 15; during operation, when inserting the reagent bottle into the support cylinder 15, first rotate the baffle 3 to move it away from above the placement hole 14, then insert the reagent bottle into the support cylinder 15. When pressing the reagent bottle down with a finger so that the top of the reagent bottle is lower than the top of the support plate 13, the baffle 3 will rotate and move above the reagent bottle under the action of the torsion spring. As the finger is removed, the baffle 3 will rotate to the position blocked by the limiting block 31. The baffle 3 is rotated to prevent the reagent bottle from detaching from the support cylinder 15. When the reagent bottle needs to be removed, the baffle 3 is rotated again to remove the baffle 3 from blocking the top of the reagent bottle. The reagent bottle will then rise a distance under the elastic force of the spring 32, and the top of the reagent bottle will protrude from the top of the placement hole 14. Through the above structure, the shaking of the reagent bottle can be further reduced after the reagent bottle is put into the box 1, and the stability of the reagent bottle inside the box 1 can be improved. At the same time, when the reagent bottle needs to be used, it can be quickly removed from the support cylinder 15, improving the ease of use of the device.

[0026] like Figures 1 to 3 As shown, a top plate 4 is fixed to the top of the spring 32; multiple sleeves 41 are fixed to the bottom of the box 1; the sleeves 41 are located outside the support cylinder 15 and surround the portions of the multiple clamping plates 16 located outside the support cylinder 15; during operation, when the reagent bottle is taken out, the top plate 4 will press against the middle of the support cylinder 15 under the elastic force of the spring 32. At this time, multiple through holes 2 are located below the top plate 4, while the placement hole 14 is located above the top plate 4. The air that enters the support cylinder 15 through the through holes 2 is unlikely to overflow to the top of the support plate 13 through the placement hole 14. Thus, when the reagent bottle is taken out, the influence of the outside air on the air temperature below the support plate 13 is reduced, thereby reducing the impact on the overall preservation of the reagent bottle.

[0027] like Figures 1 to 4As shown, a partition 5 is slidably connected to the side wall of the placement groove 21; multiple second through grooves 51 are opened in the middle of the partition 5; an elastic rope 52 is fixedly connected to the side wall of the partition 5; the top end of the elastic rope 52 is fixedly connected to the middle of the side wall of the placement groove 21; during operation, when the second cover plate 22 is closed, the second cover plate 22 will squeeze the top of the partition 5 to move the partition 5. At this time, multiple second through grooves 51 and the first through groove 23 overlap, and the air inside the through hole 2 and the air at the bottom of the support plate 13 can circulate normally. When the temperature regulating medium placed in the placement groove 21... When the medium becomes too cold to complete its task over time, the second cover plate 22 can be opened to replace the medium. At this time, the partition plate 5 will slide under the elastic force of the elastic rope 52, so that the multiple second through slots 51 and the first through slot 23 are intersected, making it difficult for the air in the open through hole 2 to circulate with the air below the support plate 13. With the above structure, when the temperature regulating medium inside the placement slot 21 is replaced, the influence of the support plate 13 on the temperature of the air below can be reduced, thereby reducing the impact on the storage temperature of the reagent bottle.

[0028] like Figure 2 As shown, multiple elastic bands 6 are fixed inside the box body 1; a counterweight 61 is fixed in the middle of the elastic band 6; during operation, when the box body 1 is moved, the elastic bands 6 and the counterweight 61 will shake, thereby disturbing the surrounding air, increasing the air flow speed at the bottom of the support plate 13 and inside the placement slot 21, making the air temperature more uniform everywhere, and improving the control effect on the temperature of reagent bottle storage.

[0029] like Figure 3 As shown, a sealing gasket 7 is fixed to the top of the top plate 4; the sealing gasket 7 is made of rubber material; through the above structure, after a reagent bottle is removed from the carrier cylinder 15, the top of the top plate 4 and the middle of the carrier cylinder 15 can be in closer contact, further reducing the flow between the outside air and the air below the carrier plate 13, and reducing heat loss.

[0030] During operation, the reagent bottle is inserted into the support cylinder 15 through the placement hole 14. After the reagent bottle enters the support cylinder 15, multiple clamps 16 will move closer together under the elastic force of the elastic membrane sleeve 17, squeezing the reagent bottle and making it difficult for thinner reagent bottles to shake inside the support cylinder 15. After placing the reagent bottle inside the box 1, a temperature regulating medium can be added to the placement slot 21. Then, the placement slot 21 is sealed with the second cover plate 22, allowing the medium to exchange heat with the air inside the placement slot 21 and the box 1. This gradually changes the temperature of the air inside the box 1 to a temperature suitable for reagent storage. The cooled air then enters the support cylinder 15 through the through hole 2. The part exchanges heat with the reagent bottle, thereby reducing the impact of temperature on reagent activity during reagent transfer. When inserting the reagent bottle into the carrier cylinder 15, first rotate the baffle 3 to move it away from above the placement hole 14. Then insert the reagent bottle into the carrier cylinder 15. When pressing the reagent bottle down with a finger so that the top of the reagent bottle is lower than the top of the carrier plate 13, the baffle 3 will rotate and move above the reagent bottle under the action of the torsion spring. As the finger is removed, the baffle 3 will rotate to the position blocked by the limiting block 31, blocking the reagent bottle and making it difficult for the reagent bottle to detach from the carrier cylinder 15. When it is necessary to remove the reagent bottle, rotate the baffle 3 again to block the baffle 3. Plate 3 no longer obstructs the top of the reagent bottle. The reagent bottle then rises a short distance under the force of spring 32, causing its top to protrude from the top of the placement hole 14. When the reagent bottle is removed, top plate 4 rests against the middle of the support cylinder 15 under the force of spring 32. At this time, multiple through holes 2 are located below top plate 4, while placement hole 14 is located above top plate 4. Air entering the support cylinder 15 through through holes 2 can no longer overflow to the top of support plate 13 through placement hole 14. When the second cover plate 22 is closed, it presses against the top of partition plate 5, causing partition plate 5 to move. At this time, multiple second through slots 51 and first through slots 23 overlap, and air inside through holes 2... The air at the bottom of the support plate 13 can circulate normally. When the temperature regulating medium placed in the placement slot 21 becomes insufficient to complete the task due to the passage of time, the second cover plate 22 can be opened to replace the medium. At this time, the partition 5 will slide under the elastic force of the elastic rope 52, so that multiple second through slots 51 and first through slots 23 are intersected, making it difficult for the air in the open through hole 2 to circulate with the air below the support plate 13. When the box 1 is moved, the elastic band 6 and the counterweight 61 will shake, thereby disturbing the surrounding air, increasing the air flow speed at the bottom of the support plate 13 and inside the placement slot 21, making the air temperature more uniform everywhere.

[0031] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A fluorescent staining kit device for detecting fungal D-glucan, comprising a box body (1); characterized in that: The top of the box (1) is rotatably connected with a first cover plate (12); the middle of the box (1) is fixedly connected with a bearing plate (13); the middle of the bearing plate (13) is provided with a plurality of mounting holes (14); the bottom of the bearing plate (13) is fixedly connected with a plurality of bearing cylinders (15); the middle of the bearing cylinder (15) is slidably connected with a plurality of clamping plates (16); the middle of the plurality of clamping plates (16) is fixedly connected with an elastic film sleeve (17).

2. The fluorescence staining kit for detecting fungal D-glucan according to claim 1, characterized in that: The middle of the bearing cylinder (15) is provided with a plurality of through holes (2); the side wall of the box (1) is provided with a placing groove (21); the top of the box (1) is rotatably connected with a second cover plate (22); the side wall of the placing groove (21) is provided with a plurality of first through grooves (23).

3. The fluorescence staining kit for detecting fungal D-glucan according to claim 1, characterized in that: The top of the bearing plate (13) is rotatably connected with a plurality of baffle plates (3) through torsional springs; the top of the box (1) is fixedly connected with a plurality of limiting blocks (31); the inner bottom of the bearing cylinder (15) is fixedly connected with a spring (32).

4. The fluorescence staining kit for detecting fungal D-glucan according to claim 3, characterized in that: The top end of the spring (32) is fixedly connected with a top plate (4); the bottom of the box (1) is fixedly connected with a plurality of sleeve shells (41); the sleeve shell (41) is located outside the bearing cylinder (15) and surrounds the part of the plurality of clamping plates (16) located outside the bearing cylinder (15).

5. The fluorescence staining kit for detecting fungal D-glucan according to claim 2, characterized in that: The side wall of the placing groove (21) is slidably connected with a partition plate (5); the middle of the partition plate (5) is provided with a plurality of second through grooves (51); the side wall of the partition plate (5) is fixedly connected with an elastic rope (52); the top end of the elastic rope (52) is fixedly connected to the middle of the side wall of the placing groove (21).

6. The fluorescence staining kit for detecting fungal D-glucan according to claim 1, characterized in that: The inside of the box (1) is fixedly connected with a plurality of elastic bands (6); the middle of the elastic band (6) is fixedly connected with a counterweight block (61).

7. The fluorescence staining kit for detecting fungal D-glucan according to claim 4, characterized in that: The top of the top plate (4) is fixedly connected with a sealing gasket (7); the sealing gasket (7) is of rubber material.

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