Rapid detection device for caries susceptibility risk

Abstract

The utility model relates to a rapid caries susceptibility risk detection device, and belongs to the technical field of detection devices.The rapid caries susceptibility risk detection device comprises a box body, the box body comprises a supporting frame and a drawing unit, the supporting frame comprises an upper-layer equipment room and a lower-layer observation room, and a camera shooting unit and an optical filter are arranged in the equipment room; the optical filter in the equipment chamber and the lens module of the camera shooting unit are coaxially arranged, so that correct filtering of light and definition of images can be guaranteed, and fluorescence emitted by a sample combined with a gram-positive bacterium targeting probe in the observation chamber is continuously shot through the camera shooting unit. The method comprises the following steps: capturing a fluorescence image of oral bacteria and recording a fluorescence intensity value, then comparing a shot sample image and the fluorescence intensity value with a standard sample image and a fluorescence intensity standard curve of healthy and already-suffered caries samples, and judging and predicting the caries occurrence possibility in the samples.

Description

Technical Field

[0001] This disclosure belongs to the field of detection device technology, specifically relating to a rapid detection device for caries susceptibility risk. Background Technology

[0002] The statements herein provide only background information in relation to this disclosure and do not necessarily constitute prior art.

[0003] Streptococcus mutans (S. mutans) is a facultative anaerobic Gram-positive bacterium considered a major pathogen of dental caries (commonly known as cavities). Dental caries is a common chronic oral disease. Currently, the most common clinical method for caries detection is probing (in dentistry, probing specifically refers to using a periodontal probe to examine periodontal tissues). However, this method lacks objectivity and cannot prevent caries in its early stages. For clinical risk assessment of caries, most methods utilize the acid-producing properties of S. mutans by culturing human dental plaque in vitro for 24-48 hours, which is time-consuming and inefficient.

[0004] The existing patent publication number CN209596421U discloses an integrated oral phototherapy device for caries monitoring and prevention. It mentions that saliva secreted by the sublingual and submandibular glands is transported to a saliva monitor to monitor saliva flow rate and saliva pH value, and to analyze whether the saliva is abnormal. However, the method of monitoring saliva by using a saliva monitor is not very accurate due to the many influencing factors of saliva pH. Moreover, it is too slow in clinical and group screening work, and cannot guide clinicians to take corresponding preventive intervention measures for patients in a timely manner, thus increasing the number of times patients need to visit the clinic. Utility Model Content

[0005] The purpose of this disclosure is to provide a rapid detection device for dental caries susceptibility risk, which can at least solve one of the above-mentioned technical problems.

[0006] To achieve the above objectives, one or more embodiments of this disclosure provide a rapid caries susceptibility risk detection device, including a housing. The housing includes a support frame and a pull-out unit. The support frame includes an upper equipment chamber and a lower observation chamber. The equipment chamber is equipped with a camera unit and a filter. A first partition is provided between the equipment chamber and the observation chamber. A guide rail for sliding the pull-out unit is provided on the bottom longitudinal frame of the support frame. The pull-out unit slides within the observation chamber.

[0007] Furthermore, the support frame is provided with side plates, tail plates, and top plates. The side plates include a first side plate and a second side plate connected to the two height edges of the tail plate. The top plates include a first top plate and a second top plate connected to the top edges of the side plates and the tail plate.

[0008] Furthermore, the first top plate and the second top plate are connected by a V-shaped bend, the first top plate is set parallel to the horizontal plane, and the second top plate is set at an acute angle to the horizontal plane in space.

[0009] Furthermore, the second top plate is provided with a control panel and an observation window from top to bottom.

[0010] Furthermore, a second partition is provided between the first partition and the first top plate, and the camera unit is located in the middle of the second partition.

[0011] Furthermore, the filter is positioned in the middle of the first partition.

[0012] Furthermore, the filter and the lens module of the camera unit are coaxially arranged.

[0013] Furthermore, the pull-out unit consists of a pull-out box and a vertical plate. The pull-out box is equipped with pulleys that cooperate with the guide rail, and the vertical plate is equipped with a pull-out handle.

[0014] Furthermore, the pull-out box is provided with a sample slot for placing samples.

[0015] Furthermore, the tail plate is provided with a heat dissipation hole for installing a cooling fan and a power hole for installing a power switch.

[0016] The beneficial effects of one or more of the above technical solutions are as follows:

[0017] This disclosure ensures proper light filtering and image clarity by coaxially arranging the filter and lens module of the camera unit within the equipment chamber. The camera unit continuously captures fluorescence emitted by samples after binding with probes targeting cariogenic bacteria within the observation chamber, recording fluorescence images and intensity values ​​of oral cariogenic bacteria. Subsequently, the captured sample images and fluorescence intensity values ​​are compared with standard sample images and fluorescence intensity standard curves of healthy and caries-infected samples to determine and predict the likelihood of caries occurrence in the samples. Attached Figure Description

[0018] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments of this application and their descriptions are used to explain this application and do not constitute a limitation thereof.

[0019] Figure 1 This is a perspective view of one or more embodiments of this disclosure from the front view direction;

[0020] Figure 2 This is a rear-view perspective view of one or more embodiments of this disclosure;

[0021] Figure 3 For one or more embodiments of this disclosure Figure 1 A cross-sectional view;

[0022] Figure 4 The following is a fluorescence imaging of different concentrations of Gram-positive bacteria using a S. mutans bacterial fluorescent probe as the detection device in one or more embodiments of this disclosure, wherein (a) is a fluorescence brightness map of different concentrations of S. mutans, and (b) is a fluorescence intensity data output map of different concentrations of S. mutans.

[0023] Figure 5 This is a comparative graph showing the assessment of the caries risk of oral saliva samples from subjects using the detection device in one or more embodiments of this disclosure, wherein (a) is a fluorescence brightness graph of oral saliva samples and (b) is a fluorescence intensity data output graph of oral saliva samples.

[0024] In the diagram, 1 is the observation window; 2 is the control panel; 3 is the first top plate; 4 is the pull-out handle; 5 is the first side plate; 6 is the tail plate; 7 is the heat dissipation hole; 8 is the power supply slot; 9 is the second top plate; 10 is the sample slot; 11 is the filter; 12 is the first partition; 13 is the second partition; 14 is the camera unit; 15 is the second side plate; 16 is the groove; 17 is the pull-out box; and 18 is the vertical plate. Detailed Implementation

[0025] like Figures 1-5 As shown, this embodiment provides a rapid detection device for dental caries susceptibility risk, including a housing, which includes a support frame and a pull-out unit.

[0026] The supporting frame includes an upper equipment room and a lower observation room. A first partition 12 is provided between the equipment room and the observation room. A filter 11 and a fluorescence excitation light source are arranged around the filter 11 on the first partition 12. The fluorescence excitation light source uses an excitation wavelength of 400 nm to 550 nm. For further optimization, the optimal excitation wavelength selected in this application is 488 nm. A light-diffusing film is also installed below the fluorescence excitation light source. The light-diffusing film corrects the diffusion angle of the light emitted by the fluorescence excitation light source, reduces the energy of the central bright spot, and expands the overall light-emitting area, making the light distribution more uniform. A guide rail for sliding the pull-out unit is provided on the longitudinal frame at the bottom of the supporting frame. The pull-out unit consists of a pull-out box 17 and a vertical plate 18. The pull-out box 17 is provided with pulleys that cooperate with the guide rail, and the vertical plate 18 is provided with a pull-out handle 4 for dragging the pull-out unit to slide in the observation room.

[0027] like Figure 3As shown, the pull-out box 17 is a sample chamber for placing samples. Inside the pull-out box 17, there is a sample slot 10 for placing samples. Various clinically suitable oral plaque collection supplies, such as 48-well cell culture plates, ELISA plates, oral swabs, cotton swabs, and dental braces, can be placed on the sample slot 10. Simultaneous testing of multiple saliva samples makes it convenient and quick to predict the disease risk of subjects, and improves the detection efficiency of clinical and population screening work.

[0028] like Figure 1 and Figure 2 As shown, the support frame is provided with side plates, tail plate 6 and top plate. The side plates include a first side plate 5 and a second side plate 15 connected to the two height edges of the tail plate. The first side plate 5 and the second side plate 15 are also provided with grooves 16 for moving the detection device, which can be easily moved through the grooves 16. The top plate includes a first top plate 3 and a second top plate 9 connected to the top edges of the side plates and the tail plate 6.

[0029] Specifically, the first top plate 3 and the second top plate 9 are connected by a V-shaped bend. The first top plate 3 is set parallel to the horizontal plane, and the second top plate 9 is set at an acute angle to the horizontal plane in space. The surface of the second top plate 9 is provided with a control plate 2 and an observation window 1 from top to bottom, which can facilitate medical staff to perform observation operations and real-time observation.

[0030] like Figure 3 As shown, a second partition 13 is provided between the first partition 12 and the first top plate 3, and an image capture unit 14 is provided in the middle of the second partition 13. In order to ensure the quality of imaging, the filter 11 provided in the middle of the first partition 12 is a filter that can filter out light up to 550 nm, especially the light from the 488 nm fluorescence excitation source. The filter 11 and the lens module of the image capture unit 14 are coaxially arranged. The sample surface is illuminated by the fluorescence excitation source, and then the image capture unit 14 captures the fluorescence emission of the saliva sample after 550 nm.

[0031] like Figure 2 As shown, the tail plate 6 is provided with a heat dissipation hole 7 for installing a cooling fan and a power supply slot 8 for installing a power switch. The heat dissipation hole 7 can effectively promote air circulation, help the equipment dissipate heat, and maintain a stable temperature during equipment operation; the power supply slot 8 ensures that the power switch is installed securely and is easy to maintain.

[0032] To verify the effectiveness of the device described in the above embodiments, the following uses a specific experiment as an example to illustrate the application process of the device in a caries susceptibility risk detection experiment:

[0033] When using this device, 300 μL of saliva was first randomly collected from the subject and placed into a 48-well cell culture plate. Then, 300 μL of 0.9% sodium chloride saline was added, followed by 0.6 μL of 7×10⁻⁶ saline solution. -3 The AI ​​E probe (AIE probe, or aggregation-induced emission probe, is a fluorescent probe with wide applications in the biomedical field) at a concentration of micromoles / liter can bind to cariogenic bacteria and emit fluorescence under light excitation from a 488 nm fluorescence excitation source (emission wavelength: 550 nm-750 nm). In this embodiment, the AI... The E probe can be the molecular probe [4-(2-{4-[bis-(4-methoxy-phenyl)-amino]-phenyl}-vinyl)-1-methyl-pyridinium]+PF6-(MeOTpy) mentioned in the existing patent CN116440077B, "An oral spray for prevention and rapid diagnosis and treatment of dental caries and its preparation method". Then, the test sample is placed in the sample chamber, and the pull-out box 17 is pushed back into the observation chamber to ensure that the sample is facing the camera unit. Next, the camera unit collects the fluorescence emission of the sample under the fluorescence excitation light source and obtains the fluorescence signal. After the image is captured, the captured sample image is observed and analyzed. The fluorescence intensity of the sample is analyzed by computer software, which can effectively determine and predict the probability of dental caries in the sample.

[0034] Specifically, to verify the feasibility of the above-mentioned experimental setup, *Streptococcus mutans* cultured in agar plates at 37 degrees Celsius for 12 hours was selected. The *Streptococcus mutans* was then mixed into physiological saline with a sodium chloride concentration of 0.9%. The *Streptococcus mutans* was quantified using a turbidimeter to obtain different concentrations (0, 10...). 7 5×10 7 10 8 2×10 8 5×10 8 10 9 1 mL of each of the *Streptococcus mutans* solutions was added. 1 μL of a 7 × 10⁻⁶ solution was added to each. -3 MeOTpy was applied in μmol / L, and the samples were then placed in quartz test vials and placed in the sample chamber to collect fluorescence emission data. Figure 4 (a) and Figure 4 As shown in (b), the fluorescence intensity increases in a gradient as the concentration of Streptococcus mutans increases, indicating that the fluorescence detection device provided in this application can detect Gram-positive bacteria at different fluorescence display concentrations.

[0035] When applied to dental caries detection, the intensity of fluorescence emission varies depending on the concentration of Streptococcus mutans in saliva, such as... Figure 5 (a) and Figure 5 As shown in (b), the detection device provided by this utility model can detect Gram-positive bacteria at different concentrations indicated by fluorescence. The saliva of subjects 3, 4, 5, 6, 8, 9, and 12 showed bright fluorescence, indicating that the concentration of Gram-positive bacteria in the oral cavity was high and there was a risk of dental caries.

[0036] While the specific embodiments of this disclosure have been described above in conjunction with the accompanying drawings, this is not intended to limit the scope of protection of this disclosure. Those skilled in the art should understand that various modifications or variations that can be made by those skilled in the art without creative effort based on the technical solutions of this disclosure are still within the scope of protection of this disclosure.

Claims

1. A device for rapid detection of caries susceptibility risk, characterized in that, The utility model provides a box body, the box body includes support frame and pull unit, the support frame includes the equipment room of upper layer and the observation room of lower layer, be provided with first partition between equipment room and observation room, be provided with guide rail for pull unit sliding on the bottom longitudinal frame of support frame, pull unit slides in observation room.

2. The device for rapid detection of caries susceptibility risk according to claim 1, characterized in that, The support frame is provided with side plates, tail plates and top plates, the side plates include first side plate and second side plate connected with two height edges of tail plate, the top plates include first top plate and second top plate connected with top edge of side plate and tail plate.

3. The device for rapid detection of caries susceptibility risk according to claim 2, characterized in that, The first top plate and the second top plate are V-shaped bending connection, the first top plate is parallelly arranged with horizontal plane, and the second top plate is arranged with an acute angle with horizontal plane in space.

4. The device for rapid detection of caries susceptibility risk according to claim 2, characterized in that, The second top plate is sequentially provided with a control panel and an observation window from top to bottom.

5. The device for rapid detection of caries susceptibility risk according to claim 1, characterized in that, The first partition and the first top plate are provided with a second partition, and a camera unit is arranged at the middle position of the second partition.

6. The device for rapid detection of caries susceptibility risk according to claim 1, characterized in that, The filter is arranged at the middle position of the first partition.

7. The device for rapid detection of caries susceptibility risk according to claim 6, characterized in that, The filter and the lens module of the camera unit are coaxially arranged.

8. The device for rapid detection of caries susceptibility risk according to claim 1, characterized in that, The pull unit is composed of a pull box and a vertical plate, the pull box is provided with a pulley matched with the guide rail, and the vertical plate is provided with a pull handle.

9. The device for rapid detection of caries susceptibility risk according to claim 8, characterized in that, The pull box is provided with a sample slot for placing samples.

10. The device for rapid detection of caries susceptibility risk according to claim 2, characterized in that, The tail plate is provided with a heat dissipation through hole for mounting a heat dissipation fan and a power supply hole for mounting a power switch.

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