Detection kit for arginine residue detection

Abstract

The utility model relates to the technical field of detection equipment, in particular to a detection kit for arginine residue detection, which comprises a pore plate for supporting and limiting a pore cup to be detected; the hole cup is used for accommodating a to-be-detected reagent or a standard reagent and is detachably mounted with the hole plate; the push-pull bottle is used for preparing and temporarily storing an unstable reagent; the conventional reagent bottle is used for storing a stable reagent; the treatment vessel is used for treating a to-be-detected sample, and the technical problems of poor detection precision and high detection cost of the arginine residue detection technology in the prior art are solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to detection equipment technical field, especially a detection kit for arginine residual detection. BACKGROUND

[0002] The description in this section only provides background information related to the present disclosure and does not constitute prior art.

[0003] In the production and manufacturing process of bone material products, arginine is often used as a sterilization protective agent, so a certain amount of arginine is left in the product composition. As an additive, the residual amount of arginine in the bone material finished product needs to be strictly controlled. In the prior art, due to the complexity of the product composition, it is difficult to detect by conventional methods due to the influence of product protein substances. Moreover, some detection components such as alpha-naphthol or arginine hydrochloride are unstable and need to be prepared for immediate use. However, the prior art requires multiple steps of taking and preparing substances, which cannot be used in time, resulting in poor detection accuracy. In addition, the number of well plates compatible with the enzyme label instrument is fixed, but the number of wells may be small during detection. Considering the pollution prevention, the well plate needs to be replaced every time it is used, resulting in high detection cost.

[0004] The prior art discloses a furanazole metabolite residual detection kit, and the novel patent with publication number CN217466936U discloses a quantitative dropper including a plurality of enzyme-labeled well plates and a quantitative dropper. The quantitative dropper is provided with a plurality of stepped quantitative hoppers, and the top of the quantitative hoppers is connected by a flow channel. A stop valve is installed at the bottom of each quantitative hopper, and a dropper is provided at the outlet of the stop valve. A plurality of test slots are formed in the enzyme-labeled well plate, and the dropper is connected to the top side of the test slot. The related technology including the above technical solution still has many problems, such as: the number of well plates used cannot be selected according to the demand, and the entire well plate must be replaced after each detection regardless of the number of well plates used, resulting in high detection cost; reagents cannot be quickly prepared, data reading time cannot be saved, and the accuracy of the product detection result is low. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a detection kit for arginine residual detection, which solves the technical problems of poor detection accuracy and high detection cost in the prior art.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0007] A detection kit for arginine residual detection, comprising:

[0008] A well plate for supporting and limiting the hole cup to be detected;

[0009] A hole cup for containing a test reagent or a standard reagent to be detected and detachably mounted on the hole plate;

[0010] A push-pull bottle for preparing and temporarily storing an unstable reagent;

[0011] A conventional reagent bottle for storing a stable reagent;

[0012] A treatment dish for treating a test sample to form a test product solution.

[0013] Further, the hole plate comprises a plate frame integrally formed and adaptively mounted on an enzyme marker, and a plurality of limiting holes are arranged on the plate frame for placing the hole cup and supporting and limiting the hole cup.

[0014] Further, the hole cup comprises a cup body with an opening on the top, and a hard clamping portion and a soft clamping portion are arranged on the peripheral wall of the cup body, the hard clamping portion is used to limit the downward movement of the hole cup when it is inserted and mounted in the limiting hole of the hole plate, and the soft clamping portion is used to limit the upward movement of the hole cup when it is inserted and mounted in the limiting hole of the hole plate.

[0015] Further, the hard clamping portion is arranged as a protrusion around the cup body or a plurality of block protrusions or a plurality of point protrusions on the same circumference, and the diameter of the protrusion is greater than the diameter of the limiting hole of the hole plate.

[0016] Further, the soft clamping portion is arranged as a sheet ring structure around the cup body or a plurality of sheet fan structures on the same circumference, and the diameter of the soft clamping portion is greater than the diameter of the limiting hole of the hole plate, and the soft clamping portion adopts a sheet deformation material.

[0017] Further, the push-pull bottle comprises a bottle body and a push plug detachably mounted on the bottle body, the peripheral wall of the bottle body is provided with a mark line, and the bottom is sealed by a diaphragm, the inner cavity wall of the bottle body is provided with an upper limiting portion and a lower limiting portion, and the push plug is used to slide and mount between the upper limiting portion and the lower limiting portion after penetrating the diaphragm.

[0018] Further, the push plug comprises a push handle and a push head, the push head is arranged as a round cake shape for sliding and sealingly mounting in the inner cavity of the bottle body, and the push handle and the push head are detachably mounted.

[0019] Further, the upper limiting portion and the lower limiting portion are both arranged as a protrusion around the bottle body or a plurality of block protrusions or a plurality of point protrusions on the same circumference, and the diameter of the protrusion is smaller than the inner diameter of the bottle body, and the upper limiting portion and the mark line are on the same horizontal plane.

[0020] Further, the hard card position part, the upper limit position part and the lower limit position part all adopt rubber material, the soft card position part adopts plastic or metal material, and the thickness of the soft card position part is 1-3mm.

[0021] Further, the push head bottom edge is provided with a plurality of anti-skid convex parts, and the anti-skid convex parts are coincided with the projection of the lower limit position part along the axial direction.

[0022] Compared with the prior art, the technical scheme of the utility model has the following beneficial effects:

[0023] (1) The utility model discloses a detachable installation of hole cup and hole plate, and the use amount of hole cup is selected according to the number of hole cups required for detection, which avoids the waste of hole plate resources caused by the pollution factor in the traditional design of hole cup and hole plate integrated type, and the position of the hole cup on the hole cup can be arranged according to the test requirement, the hole cup is removed after use, and the hole plate is cleaned and reused, so that the detection cost is greatly saved.

[0024] (2) The utility model discloses a structure of hole cup, and the hard card position part and the soft card position part are used to realize the quick installation and dismounting of the hole cup and the hole plate, so that the test time is saved, the hole cup cost is saved, and the possibility of test hole plate layout is increased.

[0025] (3) The utility model discloses a structure of push-pull bottle, and the identification line, the upper limit position part and the lower limit position part are designed and matched with the push plug, when the push-pull bottle is used for the quick preparation of unstable reagent, the push head penetrates the diaphragm and slides to the upper limit position part, so that the quantitative reagent is quickly taken, then the push head slides to the lower limit position part, the push handle is disassembled and mounted, the push-pull bottle is temporarily placed, and the next reagent preparation is carried out, so that the taking and adjusting time required by the traditional reagent preparation is greatly saved, the reagent is quickly prepared and the test is carried out, the data reading time is saved, and the accuracy of product result is improved. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 It is a three-dimensional structure schematic view of the push-pull bottle of the utility model;

[0027] Figure 2 It is a front view structure schematic view of the push-pull bottle of the utility model;

[0028] Figure 3 It is Figure 2 It is a sectional structure schematic view of A-A of the utility model;

[0029] Figure 4 It is a three-dimensional structure schematic view of the hole cup of the utility model;

[0030] Figure 5 It is a three-dimensional structure schematic view of the hole plate of the utility model;

[0031] Figure 6 As Figure 5 A cross-sectional structure schematic view at B-B;

[0032] Figure 7 As Figure 6 A local enlarged structure schematic view at C;

[0033] Figure 8 A schematic view of a three-dimensional structure of a conventional reagent bottle of the present application;

[0034] Figure 9 A schematic view of a three-dimensional structure of a treatment dish of the present application.

[0035] 100, a push-pull bottle; 101, a bottle body; 1011, an upper limiting part; 1012, a lower limiting part; 102, an identification line; 103, a push handle; 1031, a push head; 1032, an anti-skid convex part; 104, a diaphragm;

[0036] 200, a hole cup; 201, a cup body; 202, a hard card position part; 203, a soft card position part;

[0037] 300, a hole plate; 301, a plate frame; 302, a limiting hole;

[0038] 400, a conventional reagent bottle;

[0039] 500, a treatment dish. DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0041] The drawings are only used for illustrative description, and should not be understood as a limitation on the patent;

[0042] The terms used in the embodiments of the present application are only for the purpose of describing the specific embodiments, and are not intended to limit the embodiments of the present application. The singular forms "a", "an" and "the" used in the embodiments of the present application and the appended claims are also intended to include the plural forms, unless the context clearly indicates otherwise. It should also be understood that the term "and / or" used herein means and includes any or all possible combinations of one or more associated listed items.

[0043] The following description refers to the accompanying drawings. Unless otherwise noted, same or similar components in different drawings have same or similar reference numerals. The implementations described in the following exemplary embodiments are not meant to represent all implementations consistent with the present application. Rather, they are simply examples of apparatuses and methods consistent with some aspects of the present application as detailed in the appended claims.

[0044] In the description of the present application, it is understood that the terms "first", "second", "third" and the like are used only to distinguish similar objects, and do not necessarily indicate a specific order or sequence, nor can they be understood as indicating or implying relative importance. For those of ordinary skill in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0045] In addition, in the description of the present application, "a plurality of" means two or more, unless otherwise specified. The association between the associated objects is described as "and / or", which means that there can be three relationships, for example, A and / or B can mean that A exists alone, A and B exist together, and B exists alone. The character " / " generally represents an "or" relationship between the associated objects. The utility model will be further described below in conjunction with the drawings and examples.

[0046] In order to solve the limitations of the prior art, the present embodiment provides a technical solution, which will be further described below in conjunction with the drawings and examples.

[0047] The utility model mainly is to the technical improvement of the problem that the detection precision is poor and the detection cost is high in the prior art for detecting arginine residue in bone material product, because arginine is often used as an additive in bone material, but the content of arginine cannot exceed the upper limit value, so it is necessary to detect the arginine residue of finished product.

[0048] Referring to the drawings Figures 1-9The utility model provides a detection kit for arginine residual detection, include: well plate 300 for supporting and limiting the hole cup 200 of detection, well plate 300 is generally used to install the hole cup 200 of reagent preparation, then installs on the enzyme mark appearance and detects the hole cup 200, hole cup 200 is used to accommodate the reagent of detection or standard reagent and it can be detachably installed with well plate 300, push and pull bottle 100 is used to prepare and temporarily store unstable reagent for the temporary preparation before detection and then adds hole cup 200, the unstable reagent in the application is mainly alpha -naphthol and hydrochloric acid arginine, conventional reagent bottle 400 is used to store reagent, and the reagent in the application mainly includes 82% potassium hydroxide, sodium hypochlorite, alpha -naphthol and hydrochloric acid arginine, wherein 82% potassium hydroxide, sodium hypochlorite can be prepared in advance, alpha -naphthol and hydrochloric acid arginine are unstable, so need to use push and pull bottle 100 to prepare semi-finished reagent in advance, when detecting, promptly prepare finished product, then all reagent mixed liquid is added in hole cup 200, it can be understood that the above various reagents are all stored in different reagent bottles separately, processing dish (500) is used to handle the test product of detection and form the product solution of detection, the processing dish (500) is mainly used to handle the product of detection and carry out the pretreatment operation, such as grinding and then adding water to prepare solution, which is not described in detail here.

[0049] Refer to the attached drawings Figures 5-7 Well plate 300 includes a plate frame 301, which is integrally formed and adapted for installation with an enzyme marker. The plate frame 301 is provided with a plurality of limiting holes 302 for placing the hole cup 200 and supporting and limiting it. The number of limiting holes 302 in the application is commonly 96.

[0050] Refer to the attached drawings Figure 4 , Figure 6 and Figure 7, the hole cup 200 includes a cup body 201 with an opening on top, the outer wall of the cup body 201 is provided with a hard stop part 202 and a soft stop part 203, the hard stop part 202 is used to limit the downward movement of the hole cup 200 when it is inserted and installed in the limiting hole 302 of the hole plate 300, and the soft stop part 203 is used to limit the upward movement of the hole cup 200 when it is inserted and installed in the limiting hole 302 of the hole plate 300, it can be understood here that when the hole cup 200 needs to be installed on the hole plate 300, it only needs to be forced to press down, and its soft stop part 203 deforms and passes through the limiting hole 302 to restore its original state, while the hard stop part 202 is located above the limiting hole 302, so as to realize the rapid installation of the hole cup 200, when the hole cup 200 needs to be disassembled, an upward force can be applied to the hole cup 200 at the bottom of the hole plate 300 to eject it. The hard stop part 202 is provided as a protrusion around the cup body 201 or a plurality of block-shaped protrusions on the same circumference or a plurality of point-shaped protrusions on the same circumference, and the diameter of the protrusion is greater than the diameter of the limiting hole 302 of the hole plate 300, and the hard stop part 202 in the application is a protrusion around the outer wall of the cup body 201. The soft stop part 203 is provided as a sheet-shaped ring structure around the cup body 201 or a plurality of sheet-shaped sector structures on the same circumference, and the diameter of the soft stop part 203 is greater than the diameter of the limiting hole 302 of the hole plate 300, and the soft stop part 203 is made of a thin sheet of easily deformed material. The hard stop part 202, the upper limiting part 1011 and the lower limiting part 1012 are all made of rubber material, the soft stop part 203 is made of plastic or metal material, and the thickness of the soft stop part 203 is 1-3mm.

[0051] Referring to the accompanying drawings Figures 1-3The push-pull bottle 100 comprises a bottle body 101 and a push plug detachably mounted with the bottle body 101, the outer circumferential wall of the bottle body 101 is provided with a mark line 102, and the bottom is sealed by a diaphragm 104 which can be made of paper material or plastic film which is easy to penetrate, the inner cavity wall of the bottle body 101 is provided with an upper limiting portion 1011 and a lower limiting portion 1012, and the push plug is used for slidingly mounting between the upper limiting portion 1011 and the lower limiting portion 1012 after penetrating the diaphragm 104. The push plug comprises a push handle 103 and a push head 1031, the push head 1031 is provided in a circular disc shape and is slidingly and sealingly mounted in the inner cavity of the bottle body 101, the push handle 103 is detachably mounted with the push head 1031, the push handle 103 and the push head 1031 can be threadedly mounted, the push handle 103 is in a spindle shape with thin ends and a thick middle portion, facilitating gripping, and the outer circumferential surface of the push handle 103 can be provided with anti-slip convex or concave patterns, facilitating force application. The upper limiting portion 1011 and the lower limiting portion 1012 are provided with a protrusion which surrounds the bottle body 101, a plurality of block-shaped protrusions located on the same circumference or a plurality of point-shaped protrusions located on the same circumference, the diameter of the protrusion is smaller than the inner diameter of the bottle body 101, and the upper limiting portion 1011 and the mark line 102 are located on the same horizontal plane. The bottom edge of the push head 1031 is provided with a plurality of anti-slip convex portions 1032, the anti-slip convex portions 1032 are coincided with the projection of the lower limiting portion 1012 in the axial direction, the anti-slip convex portions 1032 can be rubber material sharp protrusions, the purpose is to increase the friction between the push head 1031 and the lower limiting portion 1012 when the push handle 103 needs to be detached, thereby facilitating quick detachment of the push handle 103.

[0052] When the push-pull bottle 100 is used for detecting product arginine residues, first, the product to be detected is pretreated to prepare a product to be detected solution, then 1ml of alpha-naphthol and 1ml of arginine hydrochloride are measured by using the push-pull bottle 100, the corresponding capacities of the mark line 102 and the upper limiting portion 1011 of the push-pull bottle 100 of the alpha-naphthol and the arginine hydrochloride are both 1ml, then the push handle 103 of the two push-pull bottles 100 is lowered to the lower limiting portion 1012, then the push handle 103 is removed, and the two push-pull bottles 100 are placed on the desktop, further, the hole cup 200 is placed on the hole plate 300, after all reagents are added into the hole cup 200, the alpha-naphthol and the arginine hydrochloride reagents in the push-pull bottle 100 are immediately added into the hole cup 200 after final preparation, the specific method is that the push-pull bottle containing alpha-naphthol is added with anhydrous ethanol to dissolve into 0.05% alpha-naphthol solution, the push-pull bottle containing arginine hydrochloride reagent is added with purified water to dilute to 100ug / ml, the corresponding capacity of the lower limiting portion 1012 of the push-pull bottle 100 containing alpha-naphthol is 5ml, the corresponding capacity of the lower limiting portion 1012 of the push-pull bottle 100 containing arginine hydrochloride is 10ml, and the hole cup 200 after rapid preparation can be used for residue detection, which is convenient and fast.

[0053] The above description of disclosed embodiments enables one of ordinary skill in the art to make or use the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein can be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

[0054] The above description is merely the preferred embodiments of the present application and is not intended in any way to limit the present application. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A detection kit for detecting arginine residues, characterized in that, include: The orifice plate (300) is used to support and limit the orifice cup (200) to be tested; A well cup (200) is used to hold the reagent to be tested or the standard reagent and is detachably installed from the well plate (300); Push-pull bottles (100) are used for the preparation and temporary storage of unstable reagents; Standard reagent bottles (400) are used to store stable reagents; Processing dish (500) is used to process the sample to be tested to form a solution of the product to be tested; The well plate (300) includes a plate holder (301), which is integrally formed and adapted to be installed with an ELISA reader. The plate holder (301) is provided with a plurality of limiting holes (302), which are used to place well cups (200) and support and limit them. The perforated cup (200) includes a cup body (201) with an opening at the top. The outer peripheral wall of the cup body (201) is provided with a hard locking part (202) and a soft locking part (203). The hard locking part (202) is used to restrict the downward movement of the perforated cup (200) when it is inserted into the limiting hole (302) installed in the perforated plate (300). The soft locking part (203) is used to restrict the upward movement of the perforated cup (200) when it is inserted into the limiting hole (302) installed in the perforated plate (300). The push-pull bottle (100) includes a bottle body (101) and a push plug that is detachably installed with the bottle body (101). The outer peripheral wall of the bottle body (101) is provided with a marking line (102) and the bottom is sealed with a diaphragm (104). The inner wall of the bottle body (101) is provided with an upper limit part (1011) and a lower limit part (1012) made of rubber. The push plug is used to penetrate the diaphragm (104) and slide between the upper limit part (1011) and the lower limit part (1012).

2. The detection kit for detecting arginine residues according to claim 1, characterized in that, The hard locking part (202) is configured as a protrusion that surrounds the cup body (201) for a period of time, or a number of block protrusions or a number of point protrusions located on the same circumference, and the diameter of the protrusion is larger than the diameter of the limiting hole (302) of the perforated plate (300).

3. The detection kit for detecting arginine residues according to claim 2, characterized in that, The soft locking part (203) is configured as a sheet-like circular structure that surrounds the cup body (201) or a number of sheet-like fan-shaped structures located on the same circumference, and the diameter of the soft locking part (203) is larger than the diameter of the limiting hole (302) of the perforated plate (300). The soft locking part (203) is made of a thin sheet deformable material.

4. The detection kit for detecting arginine residues according to claim 1, characterized in that, The pusher includes a push handle (103) and a push head (1031). The push head (1031) is configured as a disc-shaped object that is slidably and sealingly installed with the inner cavity of the bottle body (101). The push handle (103) and the push head (1031) are detachably installed.

5. The detection kit for detecting arginine residues according to claim 4, characterized in that, Both the upper limit part (1011) and the lower limit part (1012) are provided with a protrusion that surrounds the bottle body (101) or a number of block protrusions or a number of dot protrusions located on the same circumference. The diameter of the protrusion is smaller than the inner diameter of the bottle body (101). The upper limit part (1011) and the marking line (102) are on the same horizontal plane.

6. The detection kit for detecting arginine residues according to claim 3, characterized in that, The hard locking part (202) is made of rubber, and the soft locking part (203) is made of plastic or metal. The thickness of the soft locking part (203) is 1-3mm.

7. The detection kit for detecting arginine residues according to claim 5, characterized in that, The bottom edge of the pusher (1031) is provided with a number of anti-slip protrusions (1032), which coincide with the projection of the lower limit part (1012) along the axial direction.

Images