Host cell protein residue detection sample marking auxiliary device

Abstract

The utility model provides a host cell protein residue detection sample marking auxiliary device, and belongs to the technical field of sample detection. The host cell protein residue detection sample marking auxiliary device comprises an oscillation assembly and an auxiliary assembly, the oscillation assembly comprises an oscillator main body, a shaking table and connecting blocks, the auxiliary assembly comprises a storage rack, a shell, an insertion rod, an elastic piece, a connecting rod and a pull rod, the storage rack is arranged between the two connecting blocks, and the shell is connected with the insertion rod. By arranging the oscillator main body, the shaking table, the connecting blocks, the storage rack, the shell, the inserting rods, the elastic pieces, the connecting rods and the pull rods, the storage rack abuts against the connecting blocks at the top of the shaking table, the inserting rods are clamped into the connecting blocks to complete connection, and then the elastic pieces, the connecting rods and the pull rods are matched, so that the storage rack for containing test bottles can be conveniently loaded on the shaking table; repeated disassembly and assembly of the test bottles one by one are not needed, labor intensity is reduced, and efficiency is greatly improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of sample detection, in particular to a host cell protein residual detection sample marking auxiliary device. BACKGROUND

[0002] Host cell protein (HCP) is a process-related impurity generated in the production of recombinant biotherapeutic drugs, which remains at trace levels despite multiple downstream purification stages. The concentration of residual HCP in the preparation is often in the range of 1 ppm to 100 ppm, or even below the sub-ppm level, which may affect the quality, stability, effectiveness or safety of the product. In the detection of HCP residual, enzyme-linked immunoassay (ELISA) kit is often used. ELISA has good sensitivity, selectivity, dynamic range, sample throughput capacity and cost efficiency, however, ELISA relies on immune response and is only used for detecting specific or total HCP levels, and cannot detect non-immunoreactive proteins or identify individual proteins.

[0003] Currently, in the marking of host cell protein residual detection samples, the marker protein needs to be added to the sample and then shaken and mixed by a shaker. However, multiple test bottles containing samples are placed on a storage rack, and after the marker protein is added to each bottle, the bottles need to be fixed to the shaker one by one, and then taken down one by one after shaking and mixing, which greatly affects the experimental efficiency. CONTENT OF THE UTILITY MODEL

[0004] In order to make up for the above shortcomings, the present application provides a host cell protein residual detection sample marking auxiliary device, which aims to improve the problems raised in the above background technology.

[0005] The host cell protein residual detection sample marking auxiliary device provided by the embodiment of the present application comprises a shaking assembly and an auxiliary assembly.

[0006] The shaking assembly comprises a shaker body, a shaking table and a connecting block. The shaking table is located at the top of the shaker body, and the connecting block is arranged at the top of the shaking table.

[0007] The auxiliary assembly comprises a storage rack, a housing, a plug rod, an elastic member, a connecting rod and a pull rod. The storage rack is arranged between the two connecting blocks, the housing is arranged at the side of the storage rack, the plug rod is slidably penetrated through the housing and is inserted into the connecting block, the elastic member is sleeved on the plug rod, the pull rod is slidably penetrated through the top of the housing, and the connecting rod is hingedly connected to the plug rod and the pull rod at both ends.

[0008] In a specific embodiment, the storage rack comprises side plates, a fixed plate, an adjusting plate and a threaded rod, the fixed plate is fixedly connected to the side plates, the adjusting plate is slidingly arranged between the two side plates, the threaded rod is threaded through the fixed plate, and the bottom end of the threaded rod is slidingly rotatably threaded through the threaded rod.

[0009] In the above implementation process, by arranging the side plates, the fixed plate, the adjusting plate and the threaded rod, the distance between the adjusting plate and the fixed plate can be adjusted by rotating the threaded rod, which drives the adjusting plate to move through the threaded transmission principle.

[0010] In a specific embodiment, the fixed plate is provided with a limiting hole, and the top of the adjusting plate is provided with a limiting slot.

[0011] In the above implementation process, the limiting hole is arranged to pass through the test bottle, which is used to correspond to the placement of the test bottle and the alignment of the limiting hole.

[0012] In a specific embodiment, the bottom end of the threaded rod is provided with a limiting plate, and the top end of the threaded rod is provided with a knob.

[0013] In the above implementation process, the limiting plate is arranged to enable the threaded rod and the adjusting plate to rotate relative to each other, and the threaded rod can drive the adjusting plate to move axially.

[0014] In a specific embodiment, the elastic member comprises a fixed ring and a spring, the fixed ring is fixedly sleeved on the insertion rod, and the spring is sleeved on the insertion rod.

[0015] In the above implementation process, the fixed ring and the spring are arranged to utilize the elastic recovery of the spring to push the fixed ring, so that the fixed ring drives the insertion rod to be clamped.

[0016] In a specific embodiment, the connecting block is provided with a clamping groove, and the insertion rod is slidingly inserted into the clamping groove.

[0017] In the above implementation process, the clamping groove is arranged to enable the insertion rod to be clamped and connected by being inserted into the clamping groove.

[0018] In a specific embodiment, the shell abuts between the two connecting blocks, and the bottom of the shell is provided with a chamfer.

[0019] In the above implementation process, the chamfer is arranged to facilitate quick alignment and insertion between the connecting blocks during assembly.

[0020] In a specific embodiment, the top of the pull rod is provided with a handle.

[0021] In the above implementation process, the handle is arranged to facilitate holding and moving the pull rod.

[0022] Beneficial effects: the application provides a host cell protein residual detection sample marking auxiliary device, through setting the oscillator main body, the shaking table, the connecting block, the storage rack, the shell, the inserting rod, the elastic piece, the connecting rod and the pull rod, the storage rack is abutted between the connecting blocks on the top of the shaking table, the inserting rod is connected in the connecting block, and then the elastic piece, the connecting rod and the pull rod are matched, so that the storage rack loaded with test bottles can be conveniently loaded on the shaking table, the test bottles need not be repeatedly disassembled one by one, the labor intensity is reduced, and the efficiency is greatly improved. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the embodiments of the application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the application, and therefore should not be regarded as a limitation to the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.

[0024] Figure 1 It is a structure schematic diagram of the host cell protein residual detection sample marking auxiliary device provided by the embodiments of the application.

[0025] Figure 2 It is a structure schematic diagram of the oscillation assembly provided by the embodiments of the application.

[0026] Figure 3 It is a structure schematic diagram of the shell provided by the embodiments of the application.

[0027] Figure 4 It is a structure schematic diagram of the storage rack provided by the embodiments of the application.

[0028] Figure 5 It is a structure schematic diagram of the elastic piece provided by the embodiments of the application.

[0029] In the figure: 100-oscillation assembly; 110-oscillator main body; 120-shaking table; 130-connecting block; 131-slot; 200-auxiliary assembly; 210-storage rack; 211-side plate; 212-fixing plate; 2121-limiting hole; 213-adjusting plate; 214-threaded rod; 2141-limiting plate; 220-shell; 230-inserting rod; 240-elastic piece; 241-fixing ring; 242-spring; 250-connecting rod; 270-pull rod; 280-handle. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the application will be described clearly and completely below with reference to the drawings in the embodiments of the application. Obviously, the described embodiments are only some of the embodiments of the application, not all the embodiments.

[0031] Please refer to Figures 1-5The application provides a host cell protein residual detection sample marking auxiliary device which comprises an oscillation assembly 100 and an auxiliary assembly 200.

[0032] Please refer to Figure 1 and 2 The oscillation assembly 100 comprises a vibrator main body 110, a shaking table 120 and a connecting block 130. The shaking table 120 is located on the top of the vibrator main body 110, and the connecting block 130 is arranged on the top of the shaking table 120.

[0033] Please refer to Figure 1 、 2 , 3, 4 and 5, the auxiliary assembly 200 comprises a storage rack 210, a shell 220, a plug rod 230, an elastic member 240, a connecting rod 250 and a pull rod 270. The storage rack 210 is arranged between the two connecting blocks 130, the shell 220 is arranged on the side of the storage rack 210, the plug rod 230 is slidably arranged through the shell 220 and is inserted into the connecting block 130, the elastic member 240 is sleeved on the plug rod 230, the pull rod 270 is slidably arranged through the top of the shell 220, and the connecting rod 250 is hingedly connected to the plug rod 230 and the pull rod 270 at both ends.

[0034] The storage rack 210 comprises side plates 211, a fixed plate 212, an adjusting plate 213 and a threaded rod 214. The fixed plate 212 is fixedly connected to the side plates 211, the adjusting plate 213 is slidably arranged between the two side plates 211, and the threaded rod 214 is threadedly arranged through the fixed plate 212 and is slidably arranged through the threaded rod 214 at the bottom end. By arranging the side plates 211, the fixed plate 212, the adjusting plate 213 and the threaded rod 214, the threaded rod 214 can drive the adjusting plate 213 to move through the threaded transmission principle by rotating the threaded rod 214, and the distance between the adjusting plate 213 and the fixed plate 212 can be adjusted.

[0035] Specifically, the fixed plate 212 is provided with a limiting hole 2121, and the top of the adjusting plate 213 is provided with a limiting groove. The limiting hole 2121 is used for penetrating a test bottle and is used for aligning the test bottle with the limiting hole 2121.

[0036] It should be noted that the bottom end of the threaded rod 214 is provided with a limiting plate 2141, and the top end of the threaded rod 214 is provided with a knob. By arranging the limiting plate 2141, the threaded rod 214 and the adjusting plate 213 can be relatively rotated, and the threaded rod 214 can drive the adjusting plate 213 to move axially.

[0037] In this embodiment, the elastic member 240 comprises a fixed ring 241 and a spring 242. The fixed ring 241 is fixedly sleeved on the plug rod 230, and the spring 242 is sleeved on the plug rod 230. By arranging the fixed ring 241 and the spring 242, the fixed ring 241 is pushed by the elastic recovery action of the spring 242, so that the fixed ring 241 drives the plug rod 230 to be clamped.

[0038] In a specific embodiment, the connecting block 130 is provided with a clamping groove 131, and the insertion rod 230 is slidingly arranged in the clamping groove 131. By providing the clamping groove 131, the insertion rod 230 is inserted into the clamping groove 131 to achieve clamping connection.

[0039] In this embodiment, the shell 220 abuts between the two connecting blocks 130, and the bottom of the shell 220 is provided with a chamfer. By providing the chamfer, it is convenient to quickly align and insert between the connecting blocks 130 during assembly.

[0040] In a specific embodiment, the top of the pull rod 270 is provided with a handle 280. By providing the handle 280, it is convenient to hold the handle 280 to move the pull rod 270.

[0041] The working principle of the host cell protein residue detection sample marking auxiliary device is as follows: when in use, the sample bottle is placed in the limiting groove of the adjusting plate 213, and then the knob and the threaded rod 214 are rotated. The threaded rod 214 rotates and moves axially, the threaded rod 214 drives the adjusting plate 213 to move upwards, so that the top of the sample bottle passes through the limiting hole 2121, and then the marking protein is added to the sample bottle. Then hold the handle 280 and lift the whole to place it on the shaking bed 120. The multiple connecting blocks 130 on the top of the shaking bed 120 limit the movement of the storage rack 210, the shell 220 is inserted between the two connecting blocks 130, and then the handle 280 is released. The elastic restoring force of the spring 242 pushes the fixing ring 241, so that the fixing ring 241 drives the insertion rod 230 to insert into the clamping groove 131 to achieve clamping connection. After shaking and mixing, the handle 280 is directly held for feeding. Holding the handle 280 drives the pull rod 270 to move, the pull rod 270 drives the connecting rod 250 to move, the connecting rod 250 drives the insertion rod 230 to move out of the clamping groove 131, and then the storage rack 210 is moved upwards to be taken out, thereby greatly facilitating the removal, without repeatedly disassembling the sample bottles one by one, reducing the labor intensity, and greatly improving the efficiency.

[0042] It should be noted that the specific model and specification of the oscillator main body 110 need to be selected and determined according to the actual specification of the device. The specific selection and calculation method adopts the existing technology in the art, and therefore will not be described in detail.

[0043] The power supply of the oscillator main body 110 and its principle are clear to those skilled in the art, and will not be described in detail here.

[0044] It will be apparent to those skilled in the art that the application is not limited to the details of the above-exemplified embodiments and that the present application can be implemented in other particular forms without departing from the spirit or essential characteristics thereof. The presently disclosed embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. No reference herein to any prior art is to be taken as an admission that the application is not entitled to antedate such prior art by virtue of prior application. No reference to any prior art in this specification is intended to be, nor should it be taken as, an acknowledgement or any form of suggestion that such prior art is accepted as relevant prior art against the present application. Any citation of any prior art in this specification is solely provided on the basis of relevance to the disclosure of the application.

Claims

1. A host cell protein residue detection sample labeling auxiliary device, characterized in that, The oscillation assembly (100) includes an oscillator body (110), a shaker (120), and a connecting block (130). The shaker (120) is located on top of the oscillator body (110), and the connecting block (130) is disposed on top of the shaker (120). An auxiliary component (200) includes a storage rack (210), a housing (220), a plug rod (230), an elastic element (240), a connecting rod (250), and a pull rod (270). The storage rack (210) is disposed between two connecting blocks (130). The housing (220) is disposed on the side of the storage rack (210). The plug rod (230) slides through the housing (220) and is inserted into the connecting block (130). The elastic element (240) is sleeved on the plug rod (230). The pull rod (270) slides through the top of the housing (220). The two ends of the connecting rod (250) are respectively hinged to the plug rod (230) and the pull rod (270).

2. The host cell protein residue detection sample labeling auxiliary device according to claim 1, characterized in that, The storage rack (210) includes a side plate (211), a fixed plate (212), an adjusting plate (213), and a threaded rod (214). The fixed plate (212) is fixedly connected to the side plate (211). The adjusting plate (213) is slidably disposed between the two side plates (211). The threaded rod (214) is threaded through the fixed plate (212), and the bottom end of the threaded rod (214) slides through the threaded rod (214).

3. The host cell protein residue detection sample labeling auxiliary device according to claim 2, characterized in that, The fixing plate (212) has a limiting hole (2121), and the top of the adjusting plate (213) has a limiting groove.

4. The host cell protein residue detection sample labeling auxiliary device according to claim 3, characterized in that, The threaded rod (214) is provided with a limit plate (2141) at the bottom end and a knob at the top end.

5. The host cell protein residue detection sample labeling auxiliary device according to claim 1, characterized in that, The elastic element (240) includes a retaining ring (241) and a spring (242). The retaining ring (241) is fixedly sleeved on the insert rod (230), and the spring (242) is sleeved on the insert rod (230).

6. The host cell protein residue detection sample labeling auxiliary device according to claim 1, characterized in that, The connecting block (130) has a slot (131), and the insert rod (230) is slidably inserted into the slot (131).

7. The host cell protein residue detection sample labeling auxiliary device according to claim 1, characterized in that, The housing (220) abuts between the two connecting blocks (130), and the bottom of the housing (220) is chamfered.

8. The host cell protein residue detection sample labeling auxiliary device according to claim 1, characterized in that, The top of the pull rod (270) is provided with a handle (280).

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