Water bath oscillation device for cell resuscitation

Abstract

The utility model discloses a water bath oscillation device for cell resuscitation, which relates to the technical field of water baths, and comprises a water bath box and a top cover arranged at the top of the water bath box, a lifting frame is arranged in the water bath box, an elastic piece is fixedly arranged between the outer wall of the lifting frame and the inner wall of the water bath box, an air exhaust assembly is arranged at the top of the lifting frame, and the air exhaust assembly is connected with the top of the water bath box. The air exhaust assembly communicates with a second three-way valve, air exchanges heat with water in the water bath box in the flowing process of the internal heat exchange pipe, the air is discharged to the top of the lifting frame after being heated, and it is guaranteed that the temperature difference of the temperature of the top and the temperature of the bottom of the lifting frame is at a small numerical value; the internal temperature of the water bath box is balanced through structures such as an exhaust fan, a first air guide pipe, a first three-way valve, a seventh air guide pipe and a balance assembly, and balance of the upper temperature and the lower temperature of the cell cryopreservation container is guaranteed.

Description

TECHNICAL FIELD

[0001] The utility model relates to water bath technical field, concretely is a water bath oscillation device for cell recovery. BACKGROUND

[0002] Cell recovery, a biological term, refers to the process of recovering cell growth after the cell is thawed from liquid nitrogen or a -80 DEG C refrigerator, so that the cell cryopreservation container is heated by a heating device.

[0003] For example, the existing patent application number: 202022312205 the utility model relates to cell recovery technical field, and discloses a kind of cell recovery water bath automatic oscillation device, including box, control box is fixedly installed on the right side of the box, temperature sensor is fixedly installed on the inner bottom wall of the box, heating plate is fixedly installed on the left and right side walls of the box, support frame is fixedly connected on the top of the box, sliding slot is opened in the inner top wall of the support frame, the left side wall of the sliding slot is fixedly connected with bearing.

[0004] The above water bath oscillation device has various shortcomings during use, such as the temperature of the water bath is usually around 37℃, which is a low-temperature water bath. This results in a low heating source temperature, slow water warming speed, and poor heat flow, which can cause uneven water temperature. UTILITY MODEL CONTENT

[0005] The utility model aims to provide a water bath oscillation device for cell recovery to solve the problems in the prior art.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a water bath oscillation device for cell recovery, including a water bath tank and a top cover installed on the top of the water bath tank, the water bath tank is provided with a lifting frame inside, the outer wall of the lifting frame is fixedly installed with elastic members between the inner wall of the water bath tank, the top of the lifting frame is provided with an air extraction assembly, the air extraction assembly is communicated with a three-way valve two, the three-way valve two is installed with an aeration assembly at the normally closed end, and the three-way valve two is installed with a balancing assembly at the normally open end.

[0007] Preferably, a plurality of electric heaters are fixedly installed at the bottom of the inner cavity of the water bath tank, a vibrator is arranged at the bottom of the lifting frame, and a plurality of support columns are fixedly installed between the bottom of the vibrator and the bottom of the inner cavity of the water bath tank.

[0008] Preferably, the top cover is installed on the top of the water bath tank, and a plurality of placing grooves are formed in the top of the lifting frame.

[0009] Preferably, a plurality of guide frames are fixedly installed at the bottom of the lifting frame, a sliding frame is inserted into the guide frame, a universal joint is fixedly installed at one end of the sliding frame, and an extension rod is fixedly installed between the universal joint and the inner wall of the water bath box.

[0010] Preferably, the air extraction assembly comprises an air extractor arranged on one side of the water bath box, a guide pipe one fixedly installed at the air inlet end of the air extractor, a three-way valve one fixedly installed at one end of the guide pipe one, a guide pipe seven fixedly installed between the normally open end of the three-way valve one and the water bath box, a guide pipe two fixedly installed between the normally closed end of the three-way valve one and the water bath box, a hose fixedly installed between one end of the guide pipe two and the lifting frame, a gas guide hole arranged at the bottom of the hose, the gas guide hole being arranged on the lifting frame, and a dehumidifier fixedly installed between the air outlet end of the air extractor and a three-way valve two.

[0011] Preferably, the aeration assembly comprises a guide pipe six fixedly installed between the normally closed end of the three-way valve two and the water bath box, a shunt box two fixedly installed at one end of the guide pipe six, and a plurality of one-way valves fixedly installed at the top of the shunt box two.

[0012] Preferably, the balancing assembly comprises a guide pipe three fixedly installed between the normally open end of the three-way valve two and the water bath box, and a guide pipe five fixedly installed on one side of the water bath box, one end of the guide pipe three and one end of the guide pipe five are both fixedly installed with a shunt box one, two heat exchange pipes are fixedly installed between the two shunt boxes one, and the heat exchange pipes and the two shunt boxes one are arranged at the bottom of the inner cavity of the water bath box.

[0013] Compared with the prior art, the utility model has the advantages that:

[0014] 1. In the application, the air extractor extracts air from the top of the lifting frame through the dehumidifier, the normally open end of the three-way valve one and the guide pipe seven, the air discharged by the air extractor is dehumidified by the dehumidifier, and then returns to the top of the lifting frame through the three-way valve two, the guide pipe three, the two shunt boxes one, the two heat exchange pipes and the guide pipe six, the gas exchanges heat with the water in the water bath box during the flow in the internal heat exchange pipes, the gas is discharged to the top of the lifting frame after being heated, and the temperature difference between the top and the bottom of the lifting frame is kept at a small value; the internal temperature of the water bath box is balanced through the air extractor, the guide pipe one, the three-way valve one, the guide pipe seven, the balancing assembly and other structures, and the balance of the temperature of the cell freezing container is ensured.

[0015] 2. In the application, the air extractor extracts air from the bottom of the lifting frame through the normally closed end of the three-way valve one, the guide pipe two, the hose and the gas guide hole, the normally closed end of the three-way valve two is controlled to be opened at the same time, the air extractor supplies air to the aeration assembly through the three-way valve two, the water in the water bath box is rolled and exchanged at different distances from the lifting frame, the water in the water bath box is quickly heated to the required temperature, and problems such as slow overall water heating speed and uneven water temperature distribution caused by low heat source temperature during the process of increasing the water temperature to about 37℃ are avoided. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a partial structural schematic diagram of the water bath tank of this utility model;

[0018] Figure 3 This is a schematic diagram of the structure of the lifting frame of this utility model;

[0019] Figure 4 This is a schematic diagram of the heat exchange tube of this utility model;

[0020] Figure 5 This is a schematic diagram of the structure of the flow divider box II of this utility model;

[0021] Figure 6 This is a schematic diagram of the structure of the guide frame of this utility model.

[0022] Numbered in the diagram: 1. Water bath; 2. Top cover; 3. Exhaust fan; 4. Air duct one; 5. Three-way valve one; 6. Air duct two; 7. Hose; 8. Support frame; 9. Placement slot; 10. Elastic element; 11. Vibrator; 12. Support column; 13. Electric heater; 14. Dehumidifier; 15. Three-way valve two; 16. Air duct three; 17. Diverter box one; 18. Heat exchange tube; 20. Air duct five; 21. Air duct six; 22. Diverter box two; 23. One-way valve; 24. Guide frame; 25. Sliding frame; 26. Universal joint; 27. Telescopic rod; 28. Air duct seven; 29. ​​Air vent. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Example: Figures 1-6 As shown, this utility model provides a technical solution for a water bath shaking device for cell resuscitation, including a water bath 1 and a top cover 2 installed on the top of the water bath 1. A support frame 8 is provided inside the water bath 1. An elastic element 10 is fixedly installed between the outer wall of the support frame 8 and the inner wall of the water bath 1. An air extraction component is provided on the top of the support frame 8. The air extraction component is connected to a three-way valve 2 15. An aeration component is installed at the normally closed end of the three-way valve 2 15, and a balancing component is installed at the normally open end of the three-way valve 2 15.

[0025] Specifically, the elastic member 10 is fixedly installed between the outer wall of the lifting frame 8 and the inner wall of the water bath box 1, and can deform to reduce the passage area between the bottom and the top of the inner cavity of the water bath box 1; a plurality of guide frames 24 are fixedly installed at the bottom of the lifting frame 8, a sliding frame 25 is inserted on the guide frame 24, one end of the sliding frame 25 is fixedly installed with a universal joint 26, and a telescopic rod 27 that can be telescoped is fixedly installed between the universal joint 26 and the inner wall of the water bath box 1. Through the cooperation of the guide frame 24, the sliding frame 25, the universal joint 26 and the telescopic rod 27, a support frame that meets the up-and-down movement of the lifting frame 8 is formed.

[0026] Specifically, after opening the top cover 2 installed at the top of the water bath box 1, the cell cryopreservation container is inserted into the placing groove 9 at the top of the lifting frame 8, so that the bottom of the cell cryopreservation container extends into the water at the bottom of the inner cavity of the water bath box 1, and the top end of the cell cryopreservation container with a relatively thick diameter is clamped at the top of the lifting frame 8. The cell cryopreservation container blocks the placing groove 9, so that the space at the top of the lifting frame 8 and the space at the bottom are in a relatively sealed state.

[0027] Specifically, after completing the work of placing a plurality of cell cryopreservation containers on the lifting frame 8, the top cover 2 is closed, and then the plurality of electric heaters 13 fixedly installed at the bottom of the inner cavity of the water bath box 1 are controlled to work. The electric heaters 13 generate heat to heat the water inside the water bath box 1. The cell cryopreservation containers partially immersed in the water exchange heat with the water. Through the water bath method, the cell cryopreservation containers are heated simultaneously in a large area, ensuring the uniformity of the heating of the cell cryopreservation containers. The vibrator 11 is provided at the bottom of the lifting frame 8, and a plurality of support columns 12 are fixedly installed between the bottom of the vibrator 11 and the bottom of the inner cavity of the water bath box 1. The vibrator 11 is controlled to work, and the vibrator 11 applies a vibration force to the lifting frame 8, so that the lifting frame 8 can vibrate, and the cell solution inside the cell cryopreservation container is shaken, thereby improving the heating speed of the cell solution inside the cell cryopreservation container.

[0028] Specifically, the air extraction assembly is provided with the air extraction fan 3 outside the water bath box 1, the air extraction fan 3 is fixedly installed with the air guide pipe 4 at the air inlet end, one end of the air guide pipe 4 is fixedly installed with the three-way valve 5, the three-way valve 5 is fixedly installed with the air guide pipe 28 between the normally open end and the water bath box 1, the air guide pipe 28 is communicated with the space at the top of the lifting frame 8, the air guide pipe 6 is fixedly installed between the normally closed end of the three-way valve 5 and the water bath box 1, one end of the air guide pipe 6 is fixedly installed with the flexible pipe 7 between the lifting frame 8, and the air guide hole 29 provided at the bottom of the flexible pipe 7 is arranged on the lifting frame 8, the air guide pipe 6 and the flexible pipe 7 are communicated with the space at the bottom of the lifting frame 8; therefore, when the air extraction fan 3 is controlled to work, the air extraction fan 3 extracts air from the top of the lifting frame 8 through the air guide pipe 4, the normally open end of the three-way valve 5 and the air guide pipe 28; when the air extraction fan 3 and the three-way valve 5 are controlled to work synchronously, the normally closed end of the three-way valve 5 is opened at this time, and the air extraction fan 3 extracts air from the bottom of the lifting frame 8 through the normally closed end of the three-way valve 5, the air guide pipe 6, the flexible pipe 7 and the air guide hole 29.

[0029] Specifically, the air extraction assembly is provided with the air extraction fan 3 outside the water bath box 1, the air extraction fan 3 is fixedly installed with the air guide pipe 4 at the air inlet end, one end of the air guide pipe 4 is fixedly installed with the three-way valve 5, the three-way valve 5 is fixedly installed with the air guide pipe 28 between the normally open end and the water bath box 1, the air guide pipe 28 is communicated with the space at the top of the lifting frame 8, the air guide pipe 6 is fixedly installed between the normally closed end of the three-way valve 5 and the water bath box 1, one end of the air guide pipe 6 is fixedly installed with the flexible pipe 7 between the lifting frame 8, and the air guide hole 29 provided at the bottom of the flexible pipe 7 is arranged on the lifting frame 8, the air guide pipe 6 and the flexible pipe 7 are communicated with the space at the bottom of the lifting frame 8; therefore, when the air extraction fan 3 is controlled to work, the air extraction fan 3 extracts air from the top of the lifting frame 8 through the air guide pipe 4, the normally open end of the three-way valve 5 and the air guide pipe 28; when the air extraction fan 3 and the three-way valve 5 are controlled to work synchronously, the normally closed end of the three-way valve 5 is opened at this time, and the air extraction fan 3 extracts air from the bottom of the lifting frame 8 through the normally closed end of the three-way valve 5, the air guide pipe 6, the flexible pipe 7 and the air guide hole 29.

[0030] Specifically, the air extraction assembly is provided with the air extraction fan 3 outside the water bath box 1, the air extraction fan 3 is fixedly installed with the air guide pipe 4 at the air inlet end, one end of the air guide pipe 4 is fixedly installed with the three-way valve 5, the three-way valve 5 is fixedly installed with the air guide pipe 28 between the normally open end and the water bath box 1, the air guide pipe 28 is communicated with the space at the top of the lifting frame 8, the air guide pipe 6 is fixedly installed between the normally closed end of the three-way valve 5 and the water bath box 1, one end of the air guide pipe 6 is fixedly installed with the flexible pipe 7 between the lifting frame 8, and the air guide hole 29 provided at the bottom of the flexible pipe 7 is arranged on the lifting frame 8, the air guide pipe 6 and the flexible pipe 7 are communicated with the space at the bottom of the lifting frame 8; therefore, when the air extraction fan 3 is controlled to work, the air extraction fan 3 extracts air from the top of the lifting frame 8 through the air guide pipe 4, the normally open end of the three-way valve 5 and the air guide pipe 28; when the air extraction fan 3 and the three-way valve 5 are controlled to work synchronously, the normally closed end of the three-way valve 5 is opened at this time, and the air extraction fan 3 extracts air from the bottom of the lifting frame 8 through the normally closed end of the three-way valve 5, the air guide pipe 6, the flexible pipe 7 and the air guide hole 29.

[0031] The specific steps of the water bath heating of the cell freezing container are as follows:

[0032] In the initial stage of the multiple electric heaters 13 heating the liquid inside the water bath box 1, the control air extractor 3 and the three-way valve one 5 are controlled to work synchronously, the three-way valve one 5 works at the normally closed end to open, the air extractor 3 draws air from the bottom of the lifting frame 8 through the three-way valve one 5 normally closed end guide pipe two 6, the hose 7 and the air guide hole 29, and at the same time, the three-way valve two 15 is controlled to work at the normally closed end to open, the air extractor 3 supplies air to the aeration assembly through the three-way valve two 15, so that the water inside the water bath box 1 is stirred and exchanged at different distances from the lifting frame 8, so that the water inside the water bath box 1 is quickly heated to the required temperature, avoiding the problems of slow overall water temperature rise and uneven water temperature distribution caused by low heat source temperature during the process of raising the water temperature to about 37℃;

[0033] In the middle stage of the multiple electric heaters 13 heating the liquid inside the water bath box 1, the vibrator 11 is controlled to work to drive the lifting frame 8 to shake, at this time, the air extractor 3 and the guide pipe one 4 are controlled to work, the three-way valve one 5 is controlled to stop working at the normally closed end to close, and the three-way valve two 15 is controlled to stop working at the normally closed end to close, at this time, the air extractor 3 draws air from the top of the lifting frame 8 through the dehumidifier 14, the three-way valve one 5 normally open end and the guide pipe seven 28, the air extractor 3 exhausts the air to the top of the lifting frame 8 after the air is dehumidified by the dehumidifier 14, through the three-way valve two 15, the guide pipe three 16, the two shunt boxes one 17, the two heat exchange pipes 18 and the guide pipe five 20, the air exchanges heat with the water inside the water bath box 1 during the flow in the internal heat exchange pipe 18, and the air is discharged to the top of the lifting frame 8 after being heated, so as to ensure that the temperature difference between the top and the bottom of the lifting frame 8 is small; the air extractor 3 guide pipe one 4, the three-way valve one 5, the guide pipe seven 28, the balance assembly and other structures balance the temperature inside the water bath box 1, so as to ensure the balance of the temperature of the cell freezing container.

[0034] It is obvious for those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be regarded as exemplary and non-limiting, and the scope of the present application is defined by the appended claims rather than the above description, and all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present application. Any reference signs in the claims should not be regarded as limiting the claims involved.

Claims

1. A water bath shaking device for cell resuscitation, comprising a water bath (1) and a top cover (2) mounted on the top of the water bath (1), characterized in that: The water bath (1) is equipped with a support frame (8). An elastic element (10) is fixedly installed between the outer wall of the support frame (8) and the inner wall of the water bath (1). An air extraction component is installed on the top of the support frame (8). The air extraction component is connected to a three-way valve (15). An aeration component is installed on the normally closed end of the three-way valve (15). A balancing component is installed on the normally open end of the three-way valve (15).

2. The water bath shaking device for cell resuscitation according to claim 1, characterized in that: Multiple electric heaters (13) are fixedly installed at the bottom of the inner cavity of the water bath (1), and a vibrator (11) is provided at the bottom of the support frame (8). Multiple support columns (12) are fixedly installed between the bottom of the vibrator (11) and the bottom of the inner cavity of the water bath (1).

3. The water bath shaking device for cell resuscitation according to claim 1, characterized in that: The top cover (2) is installed on the top of the water bath (1), and the top of the support frame (8) is provided with multiple placement slots (9).

4. The water bath shaking device for cell resuscitation according to claim 1, characterized in that: The bottom of the lifting frame (8) is fixedly installed with multiple guide frames (24), and a sliding frame (25) is inserted on the guide frame (24). A universal joint (26) is fixedly installed at one end of the sliding frame (25), and a telescopic rod (27) is fixedly installed between the universal joint (26) and the inner wall of the water bath (1).

5. The water bath shaking device for cell resuscitation according to claim 1, characterized in that: The exhaust assembly includes an exhaust fan (3) installed on one side of the water bath (1), an air guide pipe (4) fixedly installed at the air inlet end of the exhaust fan (3), a three-way valve (5) fixedly installed at one end of the air guide pipe (4), and an air guide pipe (28) fixedly installed between the normally open end of the three-way valve (5) and the water bath (1). An air guide pipe (6) is fixedly installed between the normally closed end of the three-way valve (5) and the water bath (1). A flexible hose (7) is fixedly installed between one end of the air guide pipe (6) and the support frame (8). An air guide hole (29) is provided at the bottom of the flexible hose (7). The air guide hole (29) is opened on the support frame (8). A dehumidifier (14) is fixedly installed between the air outlet end of the exhaust fan (3) and the three-way valve (15).

6. The water bath shaking device for cell resuscitation according to claim 1, characterized in that: The aeration assembly includes an air guide pipe six (21) fixedly installed between the normally closed end of the three-way valve two (15) and the water bath tank (1), a diversion box two (22) fixedly installed at one end of the air guide pipe six (21), and multiple one-way valves (23) fixedly installed on the top of the diversion box two (22).

7. The water bath shaking device for cell resuscitation according to claim 1, characterized in that: The balancing assembly includes a three-way valve two (15) with a normally open end fixedly installed between the three-way valve two (15) and the water bath (1), and a five-way valve five (20) fixedly installed on one side of the water bath (1). A flow divider box one (17) is fixedly installed at one end of the three-way valve two (16) and the five-way valve five (20). Two heat exchange tubes (18) are fixedly installed between the two flow divider boxes one (17). The heat exchange tubes (18) and the two flow divider boxes one (17) are all located at the bottom of the inner cavity of the water bath (1).

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