Blood sample nucleic acid automatic processing and storing tube

Abstract

The utility model discloses an automatic blood sample nucleic acid processing and storing tube which comprises a tube body and a sealing cover covering the upper end of the tube body, and a sealing plug allowing a blood drawing needle to be inserted is arranged in the center of the sealing cover. A first insertion tube and a second insertion tube are arranged on one side of the sealing plug, penetrate through the sealing cover, extend into the inner cavity of the tube body, are provided with a first sealing head at the upper end and are in sealing connection with the sealing cover through a first sealing sleeve and a second sealing sleeve respectively, and the upper end of the first insertion tube is provided with a first sealing head; a lifting ring is arranged on the portion, located above the sealing cover, of the second insertion pipe and drives the second insertion pipe to move up and down in the inner cavity of the pipe body. According to the utility model, a blood sample in the tube body can be subjected to membrane rupture to release nucleic acid during transportation, so that the automatic treatment of the blood sample is realized, the sample detection operation steps are reduced, the detection time and the labor cost are saved, and the requirements of rapid treatment and timely detection in a laboratory are met.

Description

TECHNICAL FIELD

[0001] The utility model relates to blood sample tube technical field especially relates to a blood sample nucleic acid automatic processing storage tube. BACKGROUND

[0002] Nucleic acid detection technology has strong advantages in laboratory medicine and clinical research, blood as a common detection sample in clinic is very important in disease diagnosis and treatment, and extraction of nucleic acid from blood sample is the basis of clinical detection and biomedical research. The magnetic bead method nucleic acid extraction is a new type of nucleic acid extraction technology with nano biological magnetic beads as carrier, compared with traditional nucleic acid extraction method, the magnetic bead method does not need centrifugation and a large number of detection materials, and does not need to contact phenol / chloroform and other toxic reagents, and the operation is simple and convenient, and it is easy to realize automatic operation, and nucleic acid can be separated from blood, animal tissue, food, pathogenic microorganism and other samples automatically, and is widely used in disease control center, clinical disease diagnosis, blood transfusion safety, forensic identification and other fields.

[0003] In the nucleic acid extraction process, whole blood is collected by using a conventional blood collection tube, and then transported to a laboratory for nucleic acid extraction. The magnetic bead method nucleic acid extraction process generally includes four main steps of lysis, combination, washing and elution, and then the nucleic acid can be used for PCR amplification, enzyme cutting, molecular hybridization and the like. However, the four main steps of the magnetic bead method for extracting nucleic acid still waste a lot of time, and for a large number of blood samples, a lot of time is needed to process the blood samples, the time and labor cost consumed by nucleic acid extraction are also more, resulting in low degree of automation of blood sample processing and nucleic acid extraction, which cannot meet the needs of rapid processing and timely detection of the laboratory. UTILITY MODEL CONTENTS

[0004] To solve the technical problems in the prior art that a lot of time is needed to process blood samples, the time and labor cost consumed by nucleic acid extraction are also more, resulting in low degree of automation of blood sample processing and nucleic acid extraction, which cannot meet the needs of rapid processing and timely detection of the laboratory, the utility model provides the following technical scheme.

[0005] The utility model discloses a blood sample nucleic acid automatic processing storage tube, including the pipe body and the sealing cover of covering in the pipe body upper end, the sealing plug for drawing blood needle insertion is equipped with in the sealing cover center, one side of the sealing plug is equipped with respectively the first insertion pipe of the first sealing head of the upper end of extending to the inner chamber of the pipe body and the second insertion pipe of the upper end of being equipped with the second sealing head, the first insertion pipe and the second insertion pipe are sealed respectively with the first sealing sleeve and the second sealing sleeve and the sealing cover connection, the part of the second insertion pipe is equipped with the pull ring above the sealing cover, the pull ring drives the second insertion pipe moves up and down in the inner chamber of the pipe body.

[0006] As a further technical scheme, the outer wall of the second insertion pipe is provided with a plurality of guide sliding columns, and the inner wall of the second sealing sleeve is provided with sliding grooves matched with the guide sliding columns.

[0007] As a further technical scheme, the lower end of the first insertion pipe extends to the lower part of the pipe body.

[0008] As a further technical scheme, the outer wall of the second insertion pipe is provided with liquid outlet holes towards the axis direction of the pipe body.

[0009] As a further technical scheme, the lower part of the second insertion pipe is provided with a limiting ring for preventing the second insertion pipe from being pulled off.

[0010] As a further technical scheme, the first sealing sleeve and the second sealing sleeve are rubber sealing sleeves.

[0011] The blood sample enters the pipe body, and specific lysis liquid and specific magnetic beads can be added into the pipe body through the first insertion pipe and the second insertion pipe, so that the blood sample in the pipe body can release nucleic acid during transportation, the nucleic acid is adsorbed by the magnetic beads, the automatic processing of the blood sample can be realized, the time and labor cost of nucleic acid extraction are reduced, and the processing efficiency is improved. The pipe body can be cleaned and detected after reaching the laboratory, the sample detection operation steps are reduced, the detection time and labor cost are saved, and the demand for rapid processing and timely detection of the laboratory is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0012] Figure 1 is the external structure schematic view of the blood sample nucleic acid automatic processing and preservation pipe of the utility model;

[0013] Figure 2 is the cutaway schematic view of the blood sample nucleic acid automatic processing and preservation pipe of the utility model;

[0014] Figure 3 is Figure 2 the enlarged schematic view of A part in figure;

[0015] Figure 4 is another schematic view of the second insertion pipe of the blood sample nucleic acid automatic processing and preservation pipe of the utility model;

[0016] In the figure: 1-pipe body; 2-sealing cover; 201-sealing plug; 3-first insertion pipe; 301-first sealing head; 4-second insertion pipe; 401-second sealing head; 402-lifting ring; 403-guide sliding column; 404-liquid outlet hole; 405-limiting ring; 5-first sealing sleeve; 6-second sealing sleeve; 601-sliding groove. DETAILED DESCRIPTION

[0017] In order to make the purpose, technical scheme and advantages of the utility model clearer and more apparent, the utility model will be further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the utility model and are not used to limit the utility model. It should be noted that the examples in the utility model and the features in the examples can be combined with each other without conflict.

[0018] In the description of the utility model, it should be understood that the terms 'upper' and 'lower' are based on the orientation or positional relationship shown in the drawings, and are only used for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the utility model. In addition, the terms 'first','second', etc. are only used for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the utility model, unless otherwise specified, the meaning of 'a plurality of' is two or more.

[0019] As Figure 1 shown, the utility model discloses a blood sample nucleic acid automatic processing and preserving tube, which comprises a tube body 1 and a sealing cover 2 covering the upper end of the tube body 1, and a sealing plug 201 is arranged at the center of the sealing cover 2 and used for inserting a blood drawing needle. The tube body 1 and the sealing cover 2 adopt the existing structure of a vacuum blood drawing tube, and thus will not be described again.

[0020] As Figure 2 , Figure 3 and Figure 4 shown, in a preferred example, the sealing plug 201 is provided with a first insertion tube 3 and a second insertion tube 4 penetrating through the sealing cover 2 and extending into the inner cavity of the tube body 1 on one side, respectively. The lower end of the first insertion tube 3 extends to the lower part of the tube body 1, and specific magnetic beads can be directly added to the lower part of the tube body 1. The upper end of the first insertion tube 3 is provided with a first sealing head 301, and the upper end of the second insertion tube 4 is provided with a second sealing head 401. The first sealing head 301 and the second sealing head 401 can adopt a sealing rubber plug or a sealing screw cap structure to seal the first insertion tube 3 and the second insertion tube 4, respectively, so as to prevent the vacuum in the tube body 1 from leaking.

[0021] In a preferred embodiment, the first insertion tube 3 and the second insertion tube 4 are respectively sealedly connected with the sealing cover 2 through the first sealing sleeve 5 and the second sealing sleeve 6, that is, the sealing cover 2 is provided with a through hole, the first sealing sleeve 5 and the second sealing sleeve 6 are provided with a through hole with the same inner diameter as the sealing cover 2, and the first sealing sleeve 5 and the second sealing sleeve 6 are fixedly connected with the sealing cover 2. In the embodiment, the first sealing sleeve 5 and the second sealing sleeve 6 are rubber sealing sleeves, or the first sealing sleeve 5 and the second sealing sleeve 6 are PE sealing sleeves, and the inner walls of the first sealing sleeve 5 and the second sealing sleeve 6 are provided with rubber sealing rings. Therefore, when the first insertion tube 3 and the second insertion tube 4 are inserted into the pipe body 1 through the first sealing sleeve 5 and the second sealing sleeve 6, the vacuum in the pipe body 1 is prevented from being leaked.

[0022] In a preferred embodiment, the part of the second insertion tube 4 above the sealing cover 2 is provided with a pull ring 402, and the lower part of the second insertion tube 4 is provided with a limiting ring 405 for preventing the second insertion tube 4 from being pulled off. The pull ring 402 and the limiting ring 405 are integrally formed with the first insertion tube 4. By operating the pull ring 402, the pull ring 402 drives the second insertion tube 4 to move up and down in the inner cavity of the pipe body 1. When the second insertion tube 4 is used to inject the cell lysate into the pipe body 1, the second insertion tube 4 can move up and down while injecting the cell lysate, so that the cell lysate can fully contact with the blood sample in the pipe body 1, and the cell lysis speed in the blood sample is improved.

[0023] In a preferred embodiment, the outer wall of the second insertion tube 4 is provided with a liquid outlet hole 404 facing the axis direction of the pipe body 1. At this time, the cell lysate can not only flow into the pipe body 1 from the lower end of the second insertion tube 4, but also can flow out from the liquid outlet hole 404, so that the cell lysate can fully contact with the blood sample. The outer wall of the second insertion tube 4 is provided with a plurality of guide sliding columns 403, and the inner wall of the second sealing sleeve 6 is provided with a sliding groove 601 matched with the guide sliding columns 403. Therefore, when the second insertion tube 4 is pulled, the existence of the guide sliding columns 403 can make the second insertion tube 4 move in the vertical direction, so as to ensure the stability of the up and down movement of the second insertion tube 4 and the stability of the liquid outlet hole 404 relative to the axis direction of the pipe body 1.

[0024] In use, first, the pipe body 1 is used for normal blood sampling. After the blood sampling of the pipe body 1 is completed, the first sealing head 301 and the second sealing head 401 are opened, and the specific lysate (CelLytic cell lysate) and the specific magnetic beads (DNA extraction adsorption magnetic beads) can be respectively added into the pipe body 1 through the second insertion tube 4 and the first insertion tube 3. Then, the first sealing head 301 and the second sealing head 401 are closed. After the pipe body 1 is placed at room temperature for 2 hours and the transportation time, the cell lysate in the pipe body 1 can lyse and release nucleic acid from the cells in the blood sample. The released nucleic acid is adsorbed by the specific magnetic beads. After the sample lysing is completed, only the waste liquid is discarded, and the nucleic acid adsorbed on the magnetic beads is washed and recovered with nucleic acid-free water, and then the subsequent PCR amplification or other molecular experiments can be carried out.

[0025] The preferred specific embodiments and examples of the present application are described in detail above in conjunction with the accompanying drawings, but the present application is not limited to the above-described embodiments and examples, and various changes or equivalent replacements can be made within the knowledge possessed by those skilled in the art without departing from the concept of the present application, therefore, the present application is not limited by the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of the present application belong to the scope of the present application.

Claims

1. An automated nucleic acid processing and preservation tube for blood samples, comprising a tube body (1) and a sealing cap (2) covering the upper end of the tube body (1), wherein the sealing cap (2) has a sealing plug (201) at its center for inserting a blood-drawing needle, characterized in that: The sealing plug (201) is provided with a first insertion tube (3) extending through the sealing cap (2) to the inner cavity of the tube body (1) and a second insertion tube (4) with a first sealing head (301) at the upper end and a second sealing head (401) at the upper end. The first insertion tube (3) and the second insertion tube (4) are respectively sealed to the sealing cap (2) through a first sealing sleeve (5) and a second sealing sleeve (6). The part of the second insertion tube (4) above the sealing cap (2) is provided with a lifting ring (402). The lifting ring (402) drives the second insertion tube (4) to move up and down in the inner cavity of the tube body (1).

2. The automated nucleic acid processing and preservation tube for blood samples according to claim 1, characterized in that: The outer wall of the second insertion tube (4) is provided with a plurality of guide slides (403), and the inner wall of the second sealing sleeve (6) is provided with a slide groove (601) that matches the guide slides (403).

3. The automated nucleic acid processing and preservation tube for blood samples according to claim 1, characterized in that: The lower end of the first insertion tube (3) extends to the lower part of the tube body (1).

4. The automated nucleic acid processing and preservation tube for blood samples according to claim 1, characterized in that: The outer wall of the second insertion tube (4) is provided with an outlet hole (404) facing the axis of the tube body (1).

5. The automated blood sample nucleic acid processing and preservation tube according to claim 1, characterized in that: The lower part of the second insertion tube (4) is provided with a limiting ring (405) to prevent the second insertion tube (4) from being pulled out.

6. The automated nucleic acid processing and preservation tube for blood samples according to claim 1, characterized in that: The first sealing sleeve (5) and the second sealing sleeve (6) are rubber sealing sleeves.

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