Integrated experimental device for screening and identifying low-temperature cadmium-resistant microorganisms
By designing an integrated experimental device, the problems of cumbersome screening procedures and difficult environmental simulation in traditional low-temperature cadmium-resistant microbial screening have been solved, enabling flexible and efficient microbial screening and identification.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-25
- Publication Date
- 2026-03-24
AI Technical Summary
Traditional low-temperature screening methods for cadmium-resistant microorganisms require multiple independent steps, and it is difficult to simulate the natural low-temperature environment in the laboratory, which affects the accuracy of the screening results.
An integrated experimental device was designed, including an incubator, control panel, supplemental lighting, and camera. It can simulate a low-temperature environment and adapt to different containers by adjusting the spacing of the placement plates. Combined with a cooling mechanism, it provides stable temperature and lighting conditions and achieves automated monitoring.
It improves the flexibility and automation of low-temperature cadmium-resistant microbial screening, provides an efficient and reliable experimental platform, and ensures the accuracy of screening and identification.
Smart Images
Figure CN224031004U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of anti-cadmium microbial screening and identification, and particularly relates to an integrated experimental device for low-temperature anti-cadmium microbial screening and identification. BACKGROUND
[0002] Cadmium is a heavy metal pollutant with strong toxicity to organisms, which can affect cell metabolism, protein function and DNA stability. Low-temperature anti-cadmium microorganisms are microorganisms that can survive in low-temperature environments and have the ability to resist or reduce cadmium toxicity. According to their characteristics, and by screening and utilizing microorganisms with anti-cadmium ability, contaminated soil and water can be repaired, cadmium ions can be effectively degraded or fixed, environmental hazards can be reduced, ecological system recovery can be promoted, and biological diversity can be protected.
[0003] In the process of traditional low-temperature anti-cadmium microbial identification and screening, there are usually some deficiencies: traditional methods require multiple independent operation steps, such as sample collection, enrichment culture, separation and purification, resistance detection and molecular identification, etc. Secondly, the laboratory environment is difficult to completely simulate the low-temperature conditions in the natural environment, especially for the study of microorganisms in cold regions or special ecological systems, which affects the growth of microorganisms and the accuracy of screening results. Therefore, an integrated experimental device for low-temperature anti-cadmium microbial screening and identification is proposed to solve the above problems. UTILITY MODEL CONTENT
[0004] To solve the above problems, the utility model provides an integrated experimental device for low-temperature anti-cadmium microbial screening and identification to solve the problem.
[0005] To achieve the above purpose, the present application provides the following technical scheme:
[0006] An integrated experimental device for low-temperature anti-cadmium microbial screening and identification, comprising a incubator, the left side of the incubator is hinged connected with a box door through a door hinge, the left end of the upper surface of the incubator is fixed with a control panel, the left and right ends between the inner top wall and the inner bottom wall of the incubator are both provided with a containing mechanism, the right side of the incubator is provided with a cooling mechanism, and the left and right ends of the inner top wall of the incubator are both fixed with a light supplementing lamp.
[0007] Further settings are that the containing mechanism comprises two connecting plates fixed between the left and right ends of the opposite sides between the inner top wall and the inner bottom wall of the incubator, a plurality of clamping grooves and a plurality of insertion grooves are formed on the opposite sides of the connecting plates respectively, a placing plate is arranged on the opposite sides of the two connecting plates, cavities are formed on the left and right ends of the inner cavity of the placing plate, spring telescopic rods are fixed on the opposite sides of the cavities on the left and right ends respectively, movable lugs are fixed on the opposite sides of the spring telescopic rods on the left and right ends respectively, guide holes are formed on the left and right ends of the lower surface of the placing plate, and a supporting plate is fixed on the lower surface of the movable lug and extends to the lower side of the guide hole.
[0008] Further settings are that the size of the clamping groove cavity is matched with the size of the movable lug, and the size of the insertion groove cavity is matched with the size of the supporting plate.
[0009] Further settings are that a plurality of evenly distributed light transmission holes are formed on the left and right ends of the upper surface of the placing plate, and a label bag is fixed on the left end of the front surface of the placing plate.
[0010] Further settings are that the left and right sides of the placing plate are provided with clamping holes matched with the size of the movable lug.
[0011] Further settings are that the cross section of the supporting plate is L-shaped, and the front surface of the movable lug penetrates through the placing plate and extends to the front side of the placing plate.
[0012] Further settings are that the cooling mechanism comprises a fixed box fixed on the right side of the incubator, a fan is fixed on the left side wall of the inner cavity of the fixed box, the bottom of the right side of the fixed box is communicated with an air inlet pipe, a filter plate is arranged on the bottom of the inner cavity of the fixed box, a refrigeration fin is fixed on the top of the inner front wall of the fixed box, and a gas conveying pipe penetrates through the fixed box and extends to the inner cavity of the incubator.
[0013] Further settings are that the filter plate is located on the upper side of the air inlet pipe, sliding blocks are fixed on the bottoms of the opposite sides of the left and right side walls of the inner cavity of the fixed box, sliding grooves matched with the size of the sliding blocks are formed on the left and right sides of the filter plate, and a sealing box door is hingedly connected to the front surface of the fixed box.
[0014] Compared with the prior art, the beneficial technical effects of the utility model are that:
[0015] The containing mechanism can adjust the distance between the adjacent two placing plates to adapt to different sizes of the culture container, and the real-time monitoring of the microbial culture process is realized by combining the light supplementing lamp and the camera, so that the flexibility and the automation degree of the experiment are improved, and an efficient and reliable experimental platform is provided for the screening and identification of low-temperature cadmium-resistant microorganisms. BRIEF DESCRIPTION OF DRAWINGS
[0016] In order to more clearly illustrate the technical solutions in the specific embodiments or the prior art of the present application, the drawings needed to be used in the description of the specific embodiments or the prior art will be briefly introduced as follows. Obviously, the drawings described in the following are some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor on the basis of these drawings.
[0017] Figure 1 It is a structure schematic diagram of the present application.
[0018] Figure 2 It is a structure schematic diagram of the present application.
[0019] Figure 3 It is a structure schematic diagram of the present application.
[0020] Figure 4 It is a structure schematic diagram of the present application.
[0021] The drawings show that: 1 incubator, 2 box door, 3 control panel, 4 holding mechanism, 401 connecting plate, 402 clamping groove, 403 slot, 404 placing plate, 405 cavity, 406 spring telescopic rod, 407 movable lug, 408 guide hole, 409 support plate, 5 cooling mechanism, 501 fixed box, 502 fan, 503 air inlet pipe, 504 filter plate, 505 refrigeration fin, 506 gas pipe, 6 light supplementing lamp. DETAILED DESCRIPTION
[0022] The technical solutions of the present application will be described in detail below with reference to the drawings. Obviously, the described embodiments are some embodiments of the present application, not all embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.
[0023] In the description of the present application, it should be pointed out that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, therefore it cannot be understood as a limitation on the present application. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.
[0024] In the description of the utility model, it is necessary to explain, unless another explicit provision and limitation, the term "installation", "connection", "connection" should do the broad sense understanding, for example, can be fixed connection, also can be detachable connection, or integrally connected;Can be mechanical connection, also can be electrical connection;Can be directly connected, also can be indirectly connected through the intermediate medium, can be the communication inside two elements.For the ordinary skill in the art, the above-mentioned terms can be understood according to the specific meaning of the utility model.
[0025] Embodiment
[0026] Refer to Figures 1-4 , the utility model discloses a kind of for low-temperature cadmium-resistant microorganism screening identification integrated experimental device, including incubator 1, the left side of incubator 1 is hingedly connected with box door 2 by door hinge, the left end of the upper surface of incubator 1 is fixed with control panel 3, both ends of the left and right between the opposite side of inner top wall and inner bottom wall of incubator 1 are equipped with containing mechanism 4, the right side of incubator 1 is equipped with cooling mechanism 5, both ends of the left and right of inner top wall of incubator 1 are fixed with light supplementing lamp 6.
[0027] In the embodiment, control panel 3 is used to set temperature, illumination and ventilation parameters, light supplementing lamp 6 is opened according to setting time, and illumination is provided.
[0028] Containing mechanism 4 includes two connecting plates 401 fixed between the left and right of the opposite side of inner top wall and inner bottom wall of incubator 1, a plurality of clamping grooves 402 and a plurality of insertion grooves 403 are respectively formed on the opposite side of connecting plate 401, the opposite side of the two connecting plates 401 is equipped with placing plate 404, the left and right ends of the inner cavity of placing plate 404 are equipped with cavity 405, the opposite side of the left and right end cavities 405 is fixed with spring telescopic rod 406, the opposite side of the left and right end spring telescopic rods 406 is fixed with movable lug 407, the left and right ends of the lower surface of placing plate 404 are equipped with guide hole 408, the lower surface of movable lug 407 is fixed with support plate 409, one end of which penetrates and extends to the lower side of guide hole 408, the culture container containing microorganism is placed on placing plate 404, the height of adjacent two placing plates 404 is adjusted by the cooperation of movable lug 407 and support plate 409 with clamping groove 402 and insertion groove 403, when adjusting, the movable lug 407 moves while driving support plate 409 to move in the inner cavity of guide hole 408 by the relative motion of the left and right end movable lugs 407, at this time, the movement of movable lug 407 will cause the spring telescopic rod 406 to be extruded, so that it is shortened, until movable lug 407 moves out of clamping groove 402, support plate 409 moves out of insertion groove 403, and the placing plate 404 can be released from the limit.
[0029] The size of the inner cavity of the clamping groove 402 is matched with the size of the movable protrusion 407, the size of the inner cavity of the insertion groove 403 is matched with the size of the supporting plate 409, and a plurality of light transmission holes are uniformly arranged at the left and right ends of the upper surface of the placement plate 404, and a label bag is fixed at the left end of the front surface of the placement plate 404.
[0030] Further, the spring telescopic rod 406 is composed of a telescopic sleeve rod and a compression spring, the compression spring is movably sleeved on the outer surface of the telescopic sleeve rod, the telescopic sleeve rod is composed of a sleeve bin and a moving rod, one end of the moving rod penetrates and extends into the inside of the sleeve bin, the outer side of the moving rod is fixedly connected with a limiting block located in the inside of the sleeve bin, and a through hole matched with the moving rod is formed in one side of the sleeve bin, and the limiting block is arranged to prevent the moving rod from being separated from the sleeve bin during movement.
[0031] A spherical camera is fixed at the center of the lower surface of the placement plate 404 and the center of the top wall in the incubator 1, clamping holes matched with the size of the movable protrusion 407 are formed at the left and right sides of the placement plate 404, the cross section of the supporting plate 409 is L-shaped, and the front surface of the movable protrusion 407 penetrates through the placement plate 404 and extends to the front side of the placement plate 404.
[0032] It can be understood that the extrusion on the two movable protrusions 407 is loosened, at this time, the spring telescopic rod 406 rebounds and drives the movable protrusion 407 to move, so that the movable protrusion 407 drives the supporting plate 409 to enter the inner cavities of the clamping groove 402 and the insertion groove 403 respectively, and the position of the placement plate 404 can be fixed again.
[0033] The utility model discloses a low-temperature environment simulation device, which is used for screening and identifying microorganisms with anti-cadmium ability by simulating a low-temperature environment and providing suitable experimental conditions, and the specific process is as follows: closing the box door 2, starting the control panel 3 to set temperature, light and ventilation parameters, turning on the light supplementing lamp 6 according to the set time to provide light, and monitoring the culture process in real time through the spherical camera to record the growth state of the microorganisms; after the experiment is completed, opening the box door 2, taking out the culture container for subsequent analysis; during the screening, picking single colonies on the culture container and streaking on the culture container, and placing the culture container in the incubator 1 for culture, and repeating the above operation to obtain microorganisms with strong cadmium tolerance, and the microorganisms that can grow and reproduce normally in the cadmium-containing environment are the strains with anti-cadmium ability, and the colonies that are inhibited in growth are the microorganisms sensitive to cadmium, which can be excluded.
[0034] It should be noted that the distance between the adjacent two placement plates 404 can be adjusted through the placing mechanism 4 to adapt to culture containers of different sizes.
[0035] In order to make the temperature in the incubator 1 reach the suitable temperature, the temperature reducing mechanism 5 in the embodiment comprises a fixed box 501 fixed on the right side of the incubator 1, a fan 502 fixed on the left side wall in the inner cavity of the fixed box 501, an air inlet pipe 503 communicated with the bottom of the right side of the fixed box 501, a filter plate 504 arranged on the bottom of the inner cavity of the fixed box 501, a refrigeration fin 505 fixed on the top of the front wall of the fixed box 501, and a gas conveying pipe 506 communicated with the left side of the fixed box 501 and extending into the inner cavity of the incubator 1, wherein the fan 502 is started to suck the external air through the air inlet pipe 503 when the temperature in the incubator 1 is reduced, the air is filtered by the filter plate 504 to remove dust and impurities, the air is cooled by the refrigeration fin 505 to form cold air, and the cold air enters the incubator 1 through the gas conveying pipe 506, so that the temperature in the incubator 1 can be reduced to the set value.
[0036] In the embodiment, the filter plate 504 is located on the upper side of the air inlet pipe 503, the bottom of the opposite side of the left and right side walls in the inner cavity of the fixed box 501 is fixed with a sliding block, the left and right sides of the filter plate 504 are provided with sliding grooves matched with the size of the sliding blocks, and the front of the fixed box 501 is hingedly connected with a sealed box door.
[0037] It should be noted that the sliding blocks and the sliding grooves are arranged to facilitate the periodic replacement of the filter plate 504, so that the filtering efficiency of the filter plate 504 is not affected.
[0038] The working principle and beneficial effects of the utility model are as follows:
[0039] The culture container containing microorganisms is placed on the placing plate 404, the height of the adjacent two placing plates 404 is adjusted through the cooperation of the movable protrusions 407 and the supporting plates 409 with the clamping grooves 402 and the insertion grooves 403, when adjusting, the movable protrusions 407 at the left and right ends are relatively moved, the movable protrusions 407 are moved while driving the supporting plates 409 to move in the inner cavities of the guide holes 408, at this time, the movement of the movable protrusions 407 will cause the spring telescopic rods 406 to be extruded, so that the spring telescopic rods 406 are shortened, until the movable protrusions 407 are moved out of the clamping grooves 402, the supporting plates 409 are moved out of the insertion grooves 403, the placing plates 404 can be released from the limiting, then the positions of the placing plates 404 can be moved to the appropriate distance, and the extrusion of the two movable protrusions 407 is released, at this time, the spring telescopic rods 406 rebound and drive the movable protrusions 407 to move, so that the movable protrusions 407 drive the supporting plates 409 to enter the inner cavities of the clamping grooves 402 and the insertion grooves 403 respectively, then the box door 2 is closed, the temperature, light and ventilation parameters are set through the starting control panel 3, the light supplementing lamp 6 is turned on according to the set time to provide light, after the experiment is completed, the box door 2 is opened, and the culture container is taken out for subsequent analysis; when screening, single colonies on the culture container are picked, and are streaked on the culture container and placed in the incubator 1 for culture, and the above operation is repeated to obtain microorganisms with strong cadmium tolerance.
[0040] When the temperature in the incubator 1 is reduced, the fan 502 is started, the external air is sucked in through the air inlet pipe 503, the air is filtered through the filter plate 504 to remove dust and impurities, the air is cooled through the refrigeration fin 505 to form cold air, and the cold air enters the incubator 1 through the air conveying pipe 506, so that the temperature in the incubator 1 can be reduced to a set value.
[0041] The utility model discloses a different experimental condition, such as temperature, is set up, and the microbial sample is placed in the culture container in the incubator, and the growth condition of the microbial sample in the low temperature and the cadmium-containing environment is observed, and the microbial that grows well is the cadmium-resistant microorganism that is screened out preliminarily, and this process is screening. For example, the microorganism is placed on the culture medium of different cadmium concentrations respectively, and is cultured in the low temperature environment, and looks which microorganism can grow normally, thereby screening the microorganism with the cadmium-resistant ability.
[0042] The growth process of the microorganism is monitored in real time by using the light supplementing lamp and the camera in the device and the like equipment, and the characteristics such as the morphology and the growth rate of the microorganism are recorded, and these data can be used for further identifying the type of the microorganism and the strength of the cadmium-resistant ability. Meanwhile, after the experiment is finished, the microorganism can also be taken out from the culture container for further analysis, such as gene sequencing, to accurately identify the type of the microorganism and the cadmium-resistant mechanism and the like. For example, whether the microorganism has a specific cadmium-resistant gene is identified by observing the cell morphological change of the microorganism in the cadmium-containing environment and combining gene sequencing, so as to determine the cadmium-resistant characteristics of the microorganism.
[0043] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the utility model, and not to limit them. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part or all of the technical features. And these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the utility model.
Claims
1. An integrated experimental device for screening and identifying cadmium-resistant microorganisms at low temperatures, characterized in that, The incubator (1) is provided with a door (2) hinged to the left side of the incubator (1) via a door hinge. A control panel (3) is fixed to the left end of the upper surface of the incubator (1). A holding mechanism (4) is provided at both ends of the inner top wall and the inner bottom wall of the incubator (1). A cooling mechanism (5) is provided on the right side of the incubator (1). A supplementary light (6) is fixed at both ends of the inner top wall of the incubator (1).
2. The integrated experimental device for screening and identifying cadmium-resistant microorganisms at low temperatures according to claim 1, characterized in that, The holding mechanism (4) includes two connecting plates (401) fixed at the left and right ends between the top wall and the bottom wall of the incubator (1). The connecting plates (401) have multiple slots (402) and multiple slots (403) on their opposite sides. The two connecting plates (401) have a placement plate (404) on their opposite sides. The placement plate (404) has cavities (405) at both ends of its inner cavity. Spring telescopic rods (406) are fixed on opposite sides of the cavities (405) at both ends. Movable protrusions (407) are fixed on opposite sides of the spring telescopic rods (406) at both ends. Guide holes (408) are opened at both ends of the lower surface of the placement plate (404). A support plate (409) with one end penetrating through and extending to the lower side of the guide hole (408) is fixed on the lower surface of the movable protrusion (407).
3. The integrated experimental device for screening and identifying cadmium-resistant microorganisms at low temperatures according to claim 2, characterized in that, The dimensions of the inner cavity of the slot (402) are adapted to the dimensions of the movable protrusion (407), and the dimensions of the inner cavity of the slot (403) are adapted to the dimensions of the support plate (409).
4. The integrated experimental device for screening and identifying cadmium-resistant microorganisms at low temperatures according to claim 2, characterized in that, Multiple evenly distributed light-transmitting holes are provided on both the left and right ends of the upper surface of the placement plate (404), and a label bag is fixed on the left end of the front of the placement plate (404).
5. The integrated experimental device for screening and identifying cadmium-resistant microorganisms at low temperatures according to claim 2, characterized in that, A spherical camera is fixed at the center of the lower surface of the placement plate (404) and the center of the top wall inside the incubator (1). The left and right sides of the placement plate (404) are provided with card holes that are adapted to the size of the movable protrusion (407).
6. The integrated experimental device for screening and identifying cadmium-resistant microorganisms at low temperatures according to claim 2, characterized in that, The cross-sectional shape of the support plate (409) is L-shaped, and the front of the movable protrusion (407) penetrates through the placement plate (404) and extends to the front side of the placement plate (404).
7. The integrated experimental device for screening and identifying cadmium-resistant microorganisms at low temperatures according to claim 2, characterized in that, The cooling mechanism (5) includes a fixed box (501) fixed to the right side of the incubator (1), a fan (502) fixed to the left side wall of the inner cavity of the fixed box (501), an air inlet pipe (503) connected to the bottom of the right side of the fixed box (501), a filter plate (504) provided at the bottom of the inner cavity of the fixed box (501), a cooling plate (505) fixed to the top of the inner wall of the fixed box (501), and an air supply pipe (506) connected to the left side of the fixed box (501) with one end penetrating through the fixed box (501) and extending into the inner cavity of the incubator (1).
8. The integrated experimental device for screening and identifying cadmium-resistant microorganisms at low temperatures according to claim 7, characterized in that, The filter plate (504) is located on the upper side of the air inlet pipe (503). Slider blocks are fixed on the bottom of the opposite side of the left and right side walls of the inner cavity of the fixed box (501). Sliding grooves adapted to the size of the sliders are opened on both the left and right sides of the filter plate (504). A sealed box door is hinged to the front of the fixed box (501).