Leakage-proof structure of liquid taking opening of cell culture bag

Abstract

The utility model discloses a leakage-proof structure of a liquid taking opening of a cell culture bag, and belongs to the technical field of cell culture bags, the leakage-proof structure comprises a culture bag body, a liquid taking pipe and a silica gel plug arranged on the liquid taking pipe, and an arc-shaped groove with the high periphery and the low middle is formed in the end face, away from an opening of the liquid taking pipe, of the silica gel plug; a rotating rod extending to the arc-shaped groove is hinged to the side edge, located at the opening of the liquid taking pipe, of the silica gel plug, a sealing plug embedded with the arc-shaped groove and used for sealing the arc-shaped groove is arranged on the rotating rod, and a force applying rod used for driving the rotating rod to rotate is arranged at the end, away from the sealing plug, of the rotating rod; one end, far away from the arc-shaped groove, of the silica gel plug is provided with a warning sealing piece used for clamping and limiting the force application rod and sealing the liquid taking pipe. The device has the effects that the operation is simple and convenient, the sealing operation is prompted, and the leakage of the liquid taking opening of the cell culture bag is efficiently prevented.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell culture bags, and more particularly to a leak-proof structure for a liquid taking port of a cell culture bag. BACKGROUND

[0002] A cell culture bag is a commonly used culture container for in-vitro cell culture. Cells are placed in the cell culture bag, and the cell culture bag with the cells is placed in a culture box for culture. The cell culture bag is provided with a liquid supplementing pipe and a liquid taking pipe.

[0003] The existing cell culture bag is a sealed bag composed of a waterproof and breathable film and at least two conduits of different lengths. The conduits are a liquid supplementing pipe and a liquid taking pipe. The longer conduit is the liquid supplementing pipe, which is used for liquid supplementing and liquid pouring during cell harvesting. The liquid supplementing pipe is provided with a screw sealing cap for sealing the pipe opening. The shorter conduit is the liquid taking pipe, which is used for cell counting and liquid taking during cell culture. Since the liquid taking for counting needs to be operated multiple times, a silica gel plug is arranged at the pipe opening of the liquid taking pipe to facilitate liquid taking and prevent liquid from leaking. During the liquid taking for counting, a syringe needle is inserted into the silica gel plug for liquid taking.

[0004] However, in actual cell culture, multiple liquid taking detections are often required. After multiple insertion and extraction of the syringe needle, the silica gel plug is easily damaged, the sealing performance of the silica gel plug gradually deteriorates, liquid leakage occurs, the risk of contamination is increased, and the cell culture bag is usually placed in a carbon dioxide culture box for culture. If the cell culture bag leaks, the culture solution rich in nutrients in the cell culture bag will drip into the culture box, which will also cause microbial contamination of the entire box.

[0005] Therefore, the present application provides a leak-proof structure for a liquid taking port of a cell culture bag, which is simple to operate, prompts sealing operation, and effectively prevents liquid leakage. CONTENT OF THE UTILITY MODEL

[0006] The leak-proof structure for a liquid taking port of a cell culture bag has the advantages of being simple to operate, prompting sealing operation, and effectively preventing liquid leakage.

[0007] In order to achieve the above object, the utility model provides the following technical scheme: a cell culture bag liquid taking opening's leakage prevention structure, including culture bag body, liquid taking pipe and the silica gel plug of setting on the liquid taking pipe, the silica gel plug is away from the periphery high middle low arc groove of liquid taking pipe opening one end face setting, the side of silica gel plug is hinged and is provided with the rotary rod extending to the arc groove at the pipe orifice, be provided with the sealing plug for sealing arc groove on the rotary rod, the one end of rotary rod is away from sealing plug and is provided with the force bar for driving rotary rod rotation, the one end of silica gel plug is away from arc groove and is provided with the warning sealing element for the clamping location force bar and the sealing liquid taking pipe.

[0008] Through adopting the above technical scheme, when needing to seal the liquid taking pipe, the force bar can be applied with force to drive the rotary rod to rotate, and the rotary rod is attached to the outer wall of the silica gel plug, at this time, the sealing plug seals the arc groove, then the warning sealing element is limited to the force bar, preventing the force bar from driving the rotary rod to rotate, and the warning sealing element further seals the liquid taking pipe; when needing to open the arc groove to take liquid, the warning sealing element can be applied with force to make the warning sealing element and the force bar separate, then the rotary tube is rotated by the force bar, and the sealing plug is separated from the arc groove by the rotary rod, then the force bar is limited by the warning sealing element, preventing the force bar from rotating again, so that the liquid taking process can be carried out, and the warning sealing element can effectively prompt whether the arc groove is sealed when not taking liquid.

[0009] In summary, the arc groove and the liquid taking pipe can be effectively sealed by the force bar, the rotary tube, the sealing plug and the warning sealing element, and the warning sealing element can warn whether the arc groove is sealed, so that the operation is simple, the sealing operation is prompted, and the purpose of preventing the cell culture bag liquid taking opening from leaking is achieved.

[0010] The utility model further sets up: the warning sealing element includes two elastic clamping rods for clamping and sealing the liquid taking pipe and symmetrically setting along the extension direction of the liquid taking pipe, a force sleeve for applying opposite motion force to the two elastic clamping rods and slidingly sleeving the liquid taking pipe, a force spring for always applying force to the force sleeve in the direction of the rotary rod and sleeving the liquid taking pipe, and a light strip provided on the liquid taking pipe, one end of the force spring is connected with the liquid taking pipe, the other end is connected with the force sleeve, and the force sleeve is provided with a clamping groove for clamping the rotary rod at the end in the direction of the rotary rod.

[0011] When the sealing plug separated from the arc-shaped groove needs to be positioned, the force applying sleeve is first pulled to move towards the force applying spring and compress the force applying spring, then the sealing plug is rotated by the force applying rod, and after being rotated to a proper position, the force applying sleeve is released, and the force applying sleeve is abutted against the force applying rod under the action of the force applying spring, so that the rotation of the force applying rod is effectively avoided, and the force applying rod abuts against the force applying sleeve, avoiding the force applying sleeve from moving along the direction of the silica gel plug, so that the two elastic clamping rods are prevented from clamping and closing the liquid taking pipe under the action of the force applying sleeve, and the light emitting strip is located inside the force applying sleeve; when the sealing plug closed with the arc-shaped groove needs to be positioned, the force applying sleeve is first pulled to move towards the force applying spring and compress the force applying spring, then the sealing plug is rotated by the force applying rod and seals the arc-shaped groove, and the force applying sleeve is released, and the force applying sleeve is abutted against the hinged part of the force applying rod under the action of the force applying spring, so that the rotation of the force applying rod is effectively avoided, and the force applying sleeve moves towards the direction of the silica gel plug, and exerts opposite forces on the two elastic clamping rods, so that the liquid taking pipe is closed under the clamping of the two elastic clamping rods, so that the arc-shaped groove and the liquid taking pipe are effectively sealed, and the light emitting strip is located outside the force applying sleeve, effectively warning that the arc-shaped groove and the liquid taking pipe have been sealed.

[0012] The utility model further sets up: two elastic clamping rods are arranged in arc shape in extension direction, one end of elastic clamping rod is fixed with silica gel plug, and the other end is abutted with liquid taking pipe, the force applying sleeve is provided with the inclined groove that embeds with elastic clamping rod, and the inclined groove is arranged in the inclined way from the end close to silica gel plug to the center of liquid taking pipe close to culture bag body.

[0013] When the force applying sleeve moves along the direction of the silica gel plug, the elastic clamping rod moves along the groove wall of the inclined groove towards the direction of the liquid taking pipe under the action of the inclined groove of the force applying sleeve, and finally makes the two elastic clamping rods clamp and close the liquid taking pipe.

[0014] The utility model further sets up: the rotating rod is provided with the abutting plate that sticks to the outer wall of silica gel plug, and the force applying sleeve is provided with the limiting plate that abuts the limiting block on the outer wall of silica gel plug.

[0015] The abutting plate and the limiting plate are arranged, and the sealing plug closed with the arc-shaped groove is effectively positioned.

[0016] The utility model further sets up: the material of silica gel plug is made of polypropylene material.

[0017] In the process of the cell culture bag, the silica gel plug and the arc-shaped groove can be sterilized by burning.

[0018] In summary, the utility model has the following advantages:

[0019] 1. The arc-shaped groove and the liquid collection tube can be effectively sealed by the force-applying rod, rotating tube, sealing plug and warning seal. The warning seal also indicates whether the arc-shaped groove needs to be sealed, thus achieving the purpose of simple operation, prompting sealing operation, and effectively preventing leakage from the liquid collection port of cell culture bag.

[0020] 2. When the force-applying sleeve moves along the direction of the silicone plug, the elastic clamping rod moves along the wall of the inclined groove towards the liquid extraction tube under the action of the inclined groove of the force-applying sleeve, and finally makes the two elastic clamping rods clamp and close the liquid extraction tube.

[0021] 3. By setting up an abutment plate and a limiting plate, the sealing plug that closes with the arc-shaped groove is further effectively limited. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of this embodiment;

[0023] Figure 2 This is a partial sectional view of this embodiment;

[0024] Figure 3 This is a partial schematic diagram showing the separation of the sealing plug from the arc-shaped groove in this embodiment;

[0025] Figure 4 This is a partial schematic diagram of the sealing plug and the arc-shaped groove closing in this embodiment.

[0026] Explanation of reference numerals in the attached diagram: 1. Culture bag body; 2. Liquid collection tube; 3. Silicone stopper; 4. Arc groove; 5. Rotating rod; 6. Sealing plug; 7. Force application rod; 8. Elastic clamping rod; 9. Force application sleeve; 10. Force application spring; 11. Illuminating strip; 12. Snap-fit ​​groove; 13. Inclined groove; 14. Abutment plate; 15. Limiting plate. Detailed Implementation

[0027] The present invention will be further described in detail below with reference to the accompanying drawings.

[0028] A leak-proof structure for the liquid dispensing port of a cell culture bag, such as Figure 1 , 2 As shown, the device includes a culture bag body 1, a liquid collection tube 2, and a silicone plug 3 installed on the liquid collection tube 2. The silicone plug 3 is made of polypropylene. The silicone plug 3 has an arc-shaped groove 4 with a high periphery and a low center on the end face away from the opening of the liquid collection tube 2. A rotating rod 5 extending to the arc-shaped groove 4 is hinged to the side of the silicone plug 3 at the opening of the liquid collection tube 2. A sealing plug 6 that fits into the arc-shaped groove 4 and is used to seal the arc-shaped groove 4 is fixed on the rotating rod 5. A force-applying rod 7 for driving the rotating rod 5 to rotate is fixed to the end of the rotating rod 5 away from the sealing plug 6. A warning seal is installed on the end of the silicone plug 3 away from the arc-shaped groove 4 to lock and limit the force-applying rod 7 and seal the liquid collection tube 2.

[0029] As Figure 2 , 3 shown, the two elastic clamping rods 8 are arranged in an arc shape in the extension direction, one end of the elastic clamping rod 8 is fixed with the silica gel plug 3, the other end is in abutment with the liquid taking pipe 2, the force applying sleeve 9 is provided with an inclined groove 13 embedded with the elastic clamping rod 8, the inclined groove 13 is arranged from the end close to the silica gel plug 3 to the center of the liquid taking pipe 2 close to the culture bag body 1. When the force applying sleeve 9 moves along the direction of the silica gel plug 3, the elastic clamping rod 8 moves along the groove wall of the inclined groove 13 under the action of the inclined groove 13 of the force applying sleeve 9, and finally the two elastic clamping rods 8 clamp and close the liquid taking pipe 2.

[0030] As Figure 3 , 4 shown, the warning seal includes two elastic clamping rods 8 symmetrically arranged along the extension direction of the liquid taking pipe 2 and used for clamping and sealing the liquid taking pipe 2, a force applying sleeve 9 slidingly sleeved on the liquid taking pipe 2 and used for applying a relative motion force to the two elastic clamping rods 8, a force applying spring 10 sleeved on the liquid taking pipe 2 and always applying a force to the force applying sleeve 9 towards the direction of the rotating rod 5, and a light emitting strip 11 installed on the liquid taking pipe 2, one end of the force applying spring 10 is connected with the liquid taking pipe 2, the other end is connected with the force applying sleeve 9, and the force applying sleeve 9 is provided with a clamping groove 12 at the end close to the rotating rod 5 for clamping the rotating rod 5.

[0031] When it is needed to limit the sealing plug 6 separated from the arc-shaped groove 4, the force applying sleeve 9 can be first pulled to move towards the force applying spring 10 and compress the force applying spring 10, then the sealing plug 6 is rotated by the force applying rod 7, after being rotated to a proper position, the force applying sleeve 9 is released, the force applying sleeve 9 is in abutment with the force applying rod 7 under the action of the force applying spring 10, thereby effectively avoiding the rotation of the force applying rod 7, and the force applying rod 7 is in abutment with the force applying sleeve 9, thereby avoiding the continuous movement of the force applying sleeve 9 along the direction of the silica gel plug 3, thereby preventing the two elastic clamping rods 8 from clamping and closing the liquid taking pipe 2 under the action of the force applying sleeve 9, and at this time the light emitting strip 11 is located inside the force applying sleeve 9; when it is needed to limit the sealing plug 6 closed with the arc-shaped groove 4, the force applying sleeve 9 is first pulled to move towards the force applying spring 10 and compress the force applying spring 10, then the sealing plug 6 is rotated by the force applying rod 7 and seals the arc-shaped groove 4, the force applying sleeve 9 is released, the force applying sleeve 9 is in abutment with the hinged part of the force applying rod 7 under the action of the force applying spring 10, thereby effectively avoiding the rotation of the force applying rod 7, and the force applying sleeve 9 moves towards the direction of the silica gel plug 3 and applies a relative force to the two elastic clamping rods 8, thereby making the liquid taking pipe 2 closed under the clamping of the two elastic clamping rods 8, thereby effectively sealing the arc-shaped groove 4 and the liquid taking pipe 2, and at this time the light emitting strip 11 is located outside the force applying sleeve 9, thereby effectively warning that the arc-shaped groove 4 and the liquid taking pipe 2 have been sealed.

[0032] As Figure 3 , 4As shown, the rotating rod 5 extends with the abutting plate 14 abutting the outer wall of the silica gel plug 3, and the force applying sleeve 9 extends with the limiting plate 15 abutting the outer wall of the silica gel plug 3, further effectively limiting the sealing plug 6 closed with the arc-shaped groove 4.

[0033] The working process and beneficial effects of the utility model are as follows: when it is needed to seal the liquid taking pipe 2, an action force can be applied to the force applying rod 7 to drive the rotating rod 5 to rotate, and the rotating rod 5 is abutted with the outer wall of the silica gel plug 3, at this time, the sealing plug 6 seals the arc-shaped groove 4, then the warning sealing member is used to limit the force applying rod 7, preventing the force applying rod 7 from driving the rotating rod 5 to rotate, and the warning sealing member further seals the liquid taking pipe 2; when it is needed to open the arc-shaped groove 4 to take liquid, an action force can be applied to the warning sealing member to make the warning sealing member separate from the force applying rod 7, then the force applying rod 7 is used to drive the rotating pipe to rotate, and the rotating rod 5 drives the sealing plug 6 to separate from the arc-shaped groove 4, then the warning sealing member is used to limit the force applying rod 7, preventing the force applying rod 7 from rotating again, that is, the liquid taking process can be performed, and the warning sealing member can effectively prompt whether the arc-shaped groove 4 is sealed when the liquid is not taken.

[0034] In summary, the arc-shaped groove 4 and the liquid taking pipe 2 can be effectively sealed by the force applying rod 7, the rotating pipe, the sealing plug 6 and the warning sealing member, and the warning sealing member can warn whether the arc-shaped groove 4 is sealed, so as to achieve the purposes of simple operation, prompt sealing operation and effectively preventing the liquid taking port of the cell culture bag from leaking.

[0035] The above only describes the preferred embodiments of the utility model, and is not used to limit the utility model, any modification, equivalent replacement, improvement, etc. within the design concept of the utility model should be included in the protection scope of the utility model.

Claims

1. A leak-proof structure for the liquid dispensing port of a cell culture bag, comprising a culture bag body (1), a liquid dispensing tube (2), and a silicone plug (3) disposed on the liquid dispensing tube (2), characterized in that: The silicone plug (3) has an arc-shaped groove (4) with a high periphery and a low center on one end face away from the opening of the liquid collection tube (2). The silicone plug (3) is hinged to the side of the liquid collection tube (2) and extends to the arc-shaped groove (4). The rotating rod (5) is provided with a sealing plug (6) for sealing the arc-shaped groove (4) on the rotating rod (5). The end of the rotating rod (5) away from the sealing plug (6) is provided with a force-applying rod (7) for driving the rotating rod (5) to rotate. The end of the silicone plug (3) away from the arc-shaped groove (4) is provided with a warning seal for locking and limiting the force-applying rod (7) and sealing the liquid collection tube (2).

2. The leak-proof structure for the liquid dispensing port of a cell culture bag according to claim 1, characterized in that: The warning seal includes two elastic clamping rods (8) symmetrically arranged along the extension direction of the liquid collection tube (2) and used to clamp and seal the liquid collection tube (2); a force-applying sleeve (9) slidably sleeved on the liquid collection tube (2) and used to apply opposing forces to the two elastic clamping rods (8); a force-applying spring (10) sleeved on the liquid collection tube (2) and always applying forces to the force-applying sleeve (9) in the direction of the rotating rod (5); and a light-emitting strip (11) set on the liquid collection tube (2). One end of the force-applying spring (10) is connected to the liquid collection tube (2), and the other end is connected to the force-applying sleeve (9). The force-applying sleeve (9) is provided with a locking groove (12) for locking the rotating rod (5) at the end facing the rotating rod (5). The force of the force-applying spring (10) is greater than the deformation recovery force of the two elastic clamping rods (8).

3. The leak-proof structure for the liquid dispensing port of a cell culture bag according to claim 2, characterized in that: The two elastic clamping rods (8) are arranged in an arc shape in the extension direction. One end of the elastic clamping rod (8) is fixed to the silicone plug (3), and the other end is in contact with the liquid collection tube (2). The force application sleeve (9) is provided with an inclined groove (13) that fits into the elastic clamping rod (8). The inclined groove (13) is inclined from the end near the silicone plug (3) toward the center of the liquid collection tube (2) near the end near the culture bag body (1).

4. The leak-proof structure for the liquid dispensing port of a cell culture bag according to claim 2, characterized in that: The rotating rod (5) is provided with an abutment plate (14) that fits against the outer wall of the silicone plug (3), and the force-applying sleeve (9) is provided with a limiting plate (15) that presses the limiting block against the outer wall of the silicone plug (3).

5. The leak-proof structure for the liquid dispensing port of a cell culture bag according to claim 1, characterized in that: The silicone plug (3) is made of polypropylene.

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