Portable quantitative detection device assisted by smart phone

Abstract

The utility model discloses a portable quantitative detection device assisted by an intelligent mobile phone. The portable quantitative detection device comprises the intelligent mobile phone, an ultraviolet excitation light source, an integrated detection cabin body, a sample carrying table, a movable bottom plate, a mobile phone clamping bracket and an optical observation window, an optical observation window is formed in the middle of the integrated detection cabin, the smart phone is placed on the upper portion of the integrated detection cabin, the smart phone clamping support clamps the smart phone, a mobile phone camera of the smart phone is located on the optical observation window, a movable bottom plate is inserted into the lower portion of the integrated detection cabin, and the sample carrying table is fixed to the movable bottom plate. And a mobile phone camera of the smart phone is vertically aligned with the sample carrying table. The device realizes rapid quantitative analysis of antibiotics through the smart phone, is convenient to operate, economical, efficient and high in adaptability, can eliminate environmental interference, improves sensitivity, supports cloud data sharing while ensuring outdoor detection reliability, and facilitates efficient and accurate environment monitoring.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of fluorescence analysis equipment relates to a kind of portable quantitative detection device assisted by smart phone. BACKGROUND

[0002] Antibiotic pollution has become a global water environmental problem, traditional detection techniques such as high-performance liquid chromatography, surface-enhanced Raman scattering, liquid chromatography-tandem mass spectrometry, etc. Although they have high precision and sensitivity, they have problems such as heavy instruments, expensive equipment, complex operation requiring professional training, and organic waste liquid generation. In contrast, fluorescence sensing has high selectivity, high sensitivity, visualization, low cost and high repeatability, and is considered a popular technology for detecting antibiotics in water. In particular, the fluorescence detection system based on smart phones, by integrating professional analysis functions into mobile terminals, has achieved a major breakthrough in detection technology.

[0003] The portable detection system integrated with smart phones, combined with high-performance image processing technology, can convert the presence of antibiotics into RGB values, realizing quantitative analysis of antibiotics. The combination of smart phones and fluorescence sensing technology has the advantages of saving time and effort, low cost and not being location-dependent, and can also eliminate subjective errors in visual observation. In addition, the popularity and portability of smart phones mean that this monitoring method can be performed anytime, anywhere, and is expected to become the choice for monitoring antibiotics in water bodies. Therefore, the development of a smart phone-assisted portable fluorescence detection device can not only provide real-time, accurate and economical solutions for routine environmental monitoring, but also provide support for emergency monitoring and large-scale screening of sudden pollution incidents, achieving a technological leap from laboratory analysis to on-site instant detection. SUMMARY

[0004] The utility model aims at providing a kind of smart phone-assisted portable quantitative detection device with simple structure, easy to carry, little environmental influence, strong real-time and wide application range, to reduce dependence on large equipment and instruments.

[0005] A kind of smart phone-assisted portable quantitative detection device, including smart phone, ultraviolet excitation light source, integrated detection cabin body, sample stage, movable bottom plate, mobile phone clamping support, optical observation window;Integrated detection cabin body is provided with optical observation window in the middle, smart phone is placed on the upper portion of integrated detection cabin body, mobile phone clamping support clamps smart phone, the mobile phone camera of smart phone is located in optical observation window, movable bottom plate is inserted in the lower portion of integrated detection cabin body, sample stage is fixed on movable bottom plate, the mobile phone camera of smart phone is vertically aligned with sample stage.

[0006] The movable bottom plate side is provided with slide rail, sample stage is carried on movable bottom plate, when movable bottom plate is closed, it is ensured that sample stage is vertically aligned with optical observation window.

[0007] The inner wall of the integrated detection cabin is covered with black light-absorbing velvet to construct an optical darkroom environment.

[0008] The sample platform is made of light-transmitting and non-reflecting material, and the upper surface of the sample platform has an arc-shaped groove to meet the detection requirements of samples with different shapes and sizes.

[0009] The mobile phone clamping support has a straight sliding rail to adapt to various smart phones.

[0010] The ultraviolet flashlight of the ultraviolet excitation light source has a wavelength of 365 nm.

[0011] The utility model discloses a mobile phone clamping support is aligned with the sample platform and the optical observation window to the mobile phone camera of smart phone, and the face mode of software ColorMeter on the smart phone can realize real-time collection and RGB numerical quantitative analysis of fluorescent image.

[0012] The beneficial effects of the present application are:

[0013] The utility model discloses a smart phone realizes antibiotic rapid quantitative analysis, and the operation is convenient, and economic and efficient, and the adaptability is strong, can eliminate environmental interference, promotes sensitivity, ensures the reliability of outdoor detection, supports cloud data sharing at the same time, and it is convenient for efficient and accurate monitoring environment. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is the three-dimensional schematic diagram of the utility model;

[0015] Figure 2 It is the three-dimensional exploded schematic diagram of the utility model;

[0016] Figure 3 It is the working principle schematic diagram of the utility model;

[0017] Figure 4 It is the working curve diagram of ZET-MOG / SA fluorescent composite bead detection CTC concentration in deionized water;

[0018] Figure 5 It is the screenshot of software ColorMeter on the smart phone.

[0019] In the drawing: 1-smart phone;2-ultraviolet excitation light source;3-integrated detection cabin;4-sample platform;5-movable bottom plate;6-mobile phone clamping support;7-optical observation window;8-ZET-MOG / SA fluorescent composite bead;9-mobile phone camera;10-straight sliding rail. DETAILED DESCRIPTION

[0020] As Figure 1 , Figure 2 andFigure 3 As shown in the figure, a kind of smart phone assisted portable quantitative detection device includes smart phone 1, ultraviolet excitation light source 2, integrated detection cabin 3, sample stage 4, movable bottom plate 5, mobile phone clamping support 6, optical observation window 7;Integrated detection cabin 3 is provided with optical observation window 7 in the middle, smart phone 1 is placed on the upper part of integrated detection cabin 3, mobile phone clamping support 6 clamps smart phone 1, the mobile phone camera 9 of smart phone 1 is located in optical observation window 7, the lower part of integrated detection cabin 3 is inserted with movable bottom plate 5, sample stage 4 is fixed on movable bottom plate 5, the mobile phone camera 9 of smart phone 1 is vertically aligned with sample stage 4.

[0021] The movable bottom plate 5 side is provided with slide rail, and the sample stage 4 is carried on the movable bottom plate 5, when the movable bottom plate 5 is closed, the sample stage 4 is vertically aligned with the optical observation window 7.

[0022] The inner wall of the integrated detection cabin 3 is covered with black light-absorbing velvet, and an optical darkroom environment is constructed.

[0023] The material of the sample stage 4 is light-tight and non-reflective material, and the upper surface of the sample stage 4 has an arc-shaped groove to meet the detection requirements of samples of different shapes and sizes.

[0024] The mobile phone clamping support 6 has a straight line slide rail 10 to adapt to various smart phones 1.

[0025] The wavelength of the ultraviolet excitation light source 2 is 365 nm ultraviolet flashlight.

[0026] The mobile phone clamping support 6 aligns the mobile phone camera 9 of smart phone 1 with sample stage 4 and optical observation window 7, as shown in the figure, Figure 5 As shown in the figure, the face mode of the software ColorMeter on smart phone 1 can realize real-time acquisition and RGB numerical quantitative analysis of fluorescence image.

[0027] In order to verify the fluorescence detection effect of the utility model, ZET-MOG / SA fluorescent composite ball material is used to detect the concentration of chlortetracycline hydrochloride (CTC) in water body:

[0028] Step one: 0.6445 g of ZrOCl2·8H2O and 0.8828 g of 2,4,6-tris(4-carboxyphenyl)-1,3,5-triazine (TATB) were dissolved in 20 mL of deionized water, respectively, 1 mL of triethylamine was added to promote the dissolution of TATB, the ZrOCl2·8H2O solution and the TATB solution were mixed in a volume ratio of 1:1, the gel was formed instantaneously, and after centrifugal separation, the dry Zr-MOGs were obtained by freeze-drying. Then 2.4534 g of Eu(NO3)3·6H2O and 2.4917 g of Tb(NO3)3·6H2O were dissolved in 55 mL of ethanol, and then 0.8828 g of Zr-MOGs powder was added. After stirring for 24 h, centrifugal separation was performed, and the fluorescent ZET-MOGs powder was obtained by vacuum drying;

[0029] Step two: the fluorescent ZET-MOGs powder obtained in step one was uniformly dispersed in a 1% sodium alginate (SA) solution, and then added dropwise to a 2% CaCl2 solution by a syringe. After curing for 2 hours, the ZET-MOG / SA fluorescent composite beads 8 were obtained.

[0030] Step three: the ZET-MOG / SA fluorescent composite beads 8 obtained in step two were soaked in different concentrations of CTC solutions, such as 10 mg / L, 25 mg / L, 50 mg / L, 100 mg / L, 150 mg / L, 200 mg / L, 250 mg / L, 300 mg / L, 400 mg / L, and 500 mg / L. After taking out the ZET-MOG / SA fluorescent composite beads 8, they were placed on the sample stage 4, and the RGB values were read by using the smartphone 1. The linear relationship with the CTC concentration was established.

[0031] The results are shown in Figure 4 The standard curve can be established to realize the quantitative detection of CTC in subsequent samples, meeting the needs of instant and rapid detection.

[0032] As shown in Figure 5 The software ColorMeter on the smartphone 6 is the inherent APP of the smartphone, which is prior art.

[0033] The preparation method of the fluorescent ZET-MOGs material comprises the following steps:

[0034] Step one: weigh 0.6445 g of ZrOCl2·8H2O and place it in a glass container, add 20 mL of deionized water and ultrasonic to fully dissolve, and name it as solution A;

[0035] Step two: 0.8828 g of 2,4,6-tris (4-carboxyphenyl) -1,3,5-triazine (TATB) was weighed and placed in 20 mL of deionized water, 1 mL of triethylamine was added and ultrasonic was used to fully dissolve it, and it was named as solution B;

[0036] Step three: A and B solutions were mixed in a volume ratio of 1:1, and Zr-MOGs wet gel was quickly formed;

[0037] Step four: The obtained Zr-MOGs wet gel was washed with deionized water for 3-5 times to remove the metal ions and ligands that did not participate in coordination, and Zr-MOGs powder was obtained by freeze-drying;

[0038] Step five: 2.4534 g of Eu(NO3)3·6H2O and 2.4917 g of Tb(NO3)3·6H2O were weighed and dissolved in 55 mL of ethanol, then 0.8828 g of Zr-MOGs powder was added, stirred for 24 h, centrifuged and washed with ethanol, and finally the material was transferred to a 60℃ vacuum drying oven for 3 h to obtain a light yellow fluorescent ZET-MOGs powder, where "ZET" is the abbreviation of the element symbols Zr, Eu, Tb, and ZET-MOGs means zirconium, europium, terbium ternary metal organic gel.

Claims

1. A smartphone-assisted portable quantitative detection device, characterized in that: The application relates to a portable ultraviolet light excitation fluorescence detection device, which comprises a smart phone (1), an ultraviolet excitation light source (2), an integrated detection cabin (3), a sample stage (4), a movable bottom plate (5), a phone clamping support (6) and an optical observation window (7). 2.The smart phone-assisted portable quantitative detection device according to claim 1, wherein: The middle of the integrated detection cabin (3) is provided with the optical observation window (7), the smart phone (1) is arranged on the upper portion of the integrated detection cabin (3), the phone clamping support (6) clamps the smart phone (1), the phone camera (9) of the smart phone (1) is located at the optical observation window (7), the movable bottom plate (5) is inserted into the lower portion of the integrated detection cabin (3), the sample stage (4) is fixed on the movable bottom plate (5), and the phone camera (9) of the smart phone (1) is vertically aligned with the sample stage (4). 3.The smart phone-assisted portable quantitative detection device according to claim 1, wherein: The movable bottom plate (5) is provided with slide rails on the side. 4.The smart phone-assisted portable quantitative detection device according to claim 1, wherein: The inner wall of the integrated detection cabin (3) is covered with black light-absorbing velvet. 5.The smart phone-assisted portable quantitative detection device according to claim 1, wherein: The sample stage (4) is made of light-proof and non-reflective material. 6.The smart phone-assisted portable quantitative detection device according to claim 1, wherein: The upper surface of the sample stage (4) is provided with an arc-shaped groove. 7.The smart phone-assisted portable quantitative detection device according to claim 1, wherein: The phone clamping support (6) is provided with straight slide rails (10). The ultraviolet excitation light source (2) is a 365 nm ultraviolet flashlight.

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