Solid-phase extraction liquid adding device

Abstract

The utility model discloses a liquid adding device for solid-phase extraction. The liquid adding device comprises a fixed frame, the cooling cavity is fixed to the fixing frame, a thermoelectric refrigeration sheet and heat dissipation fins are arranged in the cooling cavity, and the cold end of the thermoelectric refrigeration sheet faces the interior of the cavity; the multi-channel liquid adding assembly comprises at least two liquid adding boxes arranged side by side, the bottom of each liquid adding box is connected with a liquid adding pipe, the liquid adding pipe penetrates through the bottom of the cooling cavity and extends to the outside, an automatic flow adjusting valve is arranged on the liquid adding pipe, and the automatic flow adjusting valve comprises a valve body, a stepping motor driving the valve body to be opened and closed and a pressure sensor detecting pipeline pressure. And the microcontroller is used for controlling the stepping motor. The utility model has the beneficial effects that according to the technical scheme, the temperature control is precise, the uninterrupted low-temperature environment is realized through thermoelectric refrigeration, and the influence of temperature fluctuation on the elution efficiency is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to solid phase extraction technical field especially, relates to a solid phase extraction liquid adding device. BACKGROUND

[0002] Solid phase extraction (SPE) is a kind of sample pretreatment technology developed in recent years, mainly used for the separation, purification and enrichment of sample. Main purpose is to reduce sample matrix interference, improve detection sensitivity, compared with traditional liquid-liquid extraction method can improve the recovery rate of analyte, mainly used for the separation, purification and concentration of sample, more effectively separate analyte and interfering component, reduce sample pretreatment process, simple operation, time-saving, labor-saving. It is widely used in medicine, food, environment, commercial inspection, chemical industry and other fields. Therefore, it is necessary to develop a kind of solid phase extraction liquid adding device, and the same technical scheme is not found by searching. INNOVATION

[0003] The utility model mainly solves the technical problem to provide a kind of solid phase extraction liquid adding device, solves one or more of the above prior art problems.

[0004] To solve the above technical problem, one technical scheme of the utility model is: a kind of solid phase extraction liquid adding device, its innovation point is: including

[0005] Fixing frame;

[0006] Cooling cavity, fixed on the fixing frame, the cooling cavity is equipped with thermoelectric refrigeration piece and radiating fin inside, the cold end of the thermoelectric refrigeration piece is towards the cavity inside;

[0007] Multi-channel liquid adding assembly, including at least two side-by-side arranged liquid adding boxes, each liquid adding box bottom is connected with liquid adding pipe, the liquid adding pipe is through the cooling cavity bottom and extends to outside, the automatic flow regulating valve is equipped on the liquid adding pipe, the automatic flow regulating valve includes valve body, the step motor of driving valve body opening and closing, pressure sensor for detecting pipeline pressure, and microcontroller for controlling step motor;

[0008] Wear-resistant transparent shell, including the box body of the cooling cavity and liquid adding box, the box body is composed of polycarbonate base material outer covering nanometer silicon dioxide coating, the liquid adding box side wall is equipped with vertical scale;

[0009] Height adjusting mechanism, including vertical fixing post, horizontal support rod and the first locking clamp and second locking clamp of connecting vertical fixing post and horizontal support rod, the first locking clamp is fixed on vertical fixing post by bolt, the second locking clamp is clamped horizontal support rod by bolt.

[0010] In some embodiments, the thermoelectric refrigeration piece is connected with an external power source, the microcontroller receives the pressure sensor signal and feedback controls the rotating speed of the stepping motor to realize constant pressure liquid feeding.

[0011] In some embodiments, the box body top of the wear-resistant transparent shell is provided with a sealing cover, the sealing cover is provided with a strip-shaped hole aligned with the liquid feeding port of the box top.

[0012] In some embodiments, the cross section of the cooling cavity is U-shaped, the liquid feeding box is embedded in the U-shaped opening, and the cold end of the thermoelectric refrigeration piece is close to the outer wall of the liquid feeding box.

[0013] In some embodiments, the first locking clamp of the height adjusting mechanism is a C-shaped clamp, the inner side is provided with an anti-skid rubber layer, and the second locking clamp is a U-shaped clamp, and the two side walls are provided with through holes for the horizontal supporting rod to pass through.

[0014] The beneficial effects of the present application are: the temperature control is accurate, the thermoelectric refrigeration realizes uninterrupted low-temperature environment, and the influence of temperature fluctuation on elution efficiency is avoided. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor, wherein:

[0016] Figure 1 is a structural schematic view of a solid phase extraction liquid feeding device.

[0017] Figure 2 is a side view of a solid phase extraction liquid feeding device.

[0018] Figure 3 is Figure 2 A-A sectional view.

[0019] Figure 4 is a temperature control and flow control flow chart of a solid phase extraction liquid feeding device. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only some embodiments of the present application, not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0021] AsFigures 1 to 4 The utility model discloses an embodiment shown below,

[0022] 1. The overall structure of the device

[0023] The utility model relates to a kind of solid phase extraction liquid adding device, mainly including following core components: fixed frame 100: by bottom plate, column 201 and horizontal support 204, bottom plate bottom is equipped with antiskid support block, column 201 is vertically fixed on bottom plate, horizontal support 204 is connected with column 201 by locking clamp, height adjustment is realized.

[0024] Cooling cavity 500: fixed on horizontal support 204, cavity interior is integrated with thermoelectric refrigeration piece and radiating fin, the cold end of thermoelectric refrigeration piece is towards cavity inner wall, and the hot end is exchanged heat with external air through radiating fin, forming active cooling system.

[0025] Multi-channel liquid adding component: contain multiple side-by-side arranged liquid adding box 301, each liquid adding box 301 bottom is connected with liquid adding pipe 302, liquid adding pipe 302 extends to cooling cavity 500 outside, and automatic flow regulating valve 303 is equipped on pipeline.

[0026] Wear-resistant transparent shell 400: wrapping cooling cavity 500 and liquid adding box 301, adopt polycarbonate base material outer covering nanometer silicon dioxide coating, with high transparency and scratch resistance, and the side wall of liquid adding box 301 is equipped with accurate scale.

[0027] Height adjustment mechanism: including C-shaped locking clamp on column 201 and U-shaped locking clamp on horizontal support 204, fixed by bolt, support the overall height of device to be adjusted quickly to adapt to different models of solid phase extraction instrument.

[0028] 2. Detailed description of each component and working principle

[0029] (1) thermoelectric refrigeration and cooling cavity 500

[0030] Structural design: cooling cavity 500 cross section is U-shaped, and thermoelectric refrigeration piece is embedded inside, and the cold end is tightly attached to the outer wall of liquid adding box 301, and the hot end is connected with radiating fin;The top of cavity is equipped with sealing cover (not shown), and strip hole is opened on cover surface and is aligned with liquid adding box 301 top liquid adding port.

[0031] Working principle: after thermoelectric refrigeration piece is electrified, heat is transferred from cold end to hot end through peltier effect, so that the temperature of eluent in liquid adding box 301 is reduced;Radiating fin is discharged by natural convection or forced air cooling.

[0032] Synergistic relationship: U-shaped cavity design ensures that liquid adding box 301 is fully wrapped by refrigeration piece, and the uniformity of cooling is improved;Sealing cover reduces the loss of cold, and strip hole allows to supplement eluent without opening cover.

[0033] Advantages: Compared with traditional ice bags, the temperature control is more stable, and there is no need to frequently replace the refrigerant medium, which is suitable for long-term experiments.

[0034] (2) Automatic flow regulating valve 303

[0035] Structural design: The valve body is internally provided with a valve core driven by a stepping motor, a pressure sensor is integrated on the pipeline, and a microcontroller receives the sensor signal and controls the rotating speed of the stepping motor.

[0036] Working principle: The pressure sensor monitors the liquid pressure in the liquid feeding pipe 302 in real time, and the microcontroller adjusts the opening degree of the valve core according to the preset pressure value to realize constant-pressure liquid feeding; for example, when the viscosity of the eluent changes, the system automatically compensates for the flow fluctuation.

[0037] Synergistic relationship: Cooperating with the multi-channel liquid feeding assembly, the flow rate of each channel can be independently controlled, supporting parallel processing of multiple samples; the microcontroller supports programmatic setting of liquid feeding parameters at different stages (such as gradient elution).

[0038] Advantages: Eliminate manual adjustment errors and improve experimental repeatability; reduce solvent waste and reduce human operation burden.

[0039] (3) Wear-resistant transparent shell 400

[0040] Material properties: The polycarbonate substrate provides high impact resistance, and the nano-silica coating enhances the surface hardness (Mohs hardness ≥ 7) and resists common chemical attacks in the experimental environment.

[0041] Structural design: The shell top sealing cover is tightly fitted with the U-shaped cooling cavity 500, and the laser etching process is used for the side wall scale of the liquid feeding box 301 to ensure that it does not become blurred during long-term use.

[0042] Synergistic relationship: Transparent design facilitates observation of liquid level and cooling fin working status; wear-resistant coating reduces surface scratches caused by frequent wiping, avoiding scale reading errors.

[0043] Advantages: Prolong the service life of the device and ensure the accuracy of experimental data.

[0044] (4) Height adjusting mechanism

[0045] Operation process: Loosen the bolts of the C-shaped locking clamp to adjust the height of the vertical column 201 and the horizontal support rod 204, and tighten the bolts after adjustment; the U-shaped locking clamp clamps the horizontal support rod 204 through the through hole to ensure the stability of the device.

[0046] Synergistic relationship: Height adjustment allows the liquid feeding pipe 302 outlet to accurately align with solid phase extraction columns of different heights, avoiding liquid splashing or misalignment.

[0047] Advantages: Adapt to various laboratory equipment layouts, improve device versatility.

[0048] 3. Typical application scenarios and operation examples

[0049] Scenario one: multi-batch sample processing

[0050] The experimenter injects 6 liquid addition boxes 301 with different eluents respectively, and presets the flow rate of each channel (such as 0.5 mL / min) through a microcontroller.

[0051] After the sealing cover is closed, the thermoelectric cooling fin is started to maintain the eluent temperature below 4°C to reduce volatilization.

[0052] The device height is adjusted to be 5 cm above the solid-phase extraction column, automatic liquid addition is started, and the system adjusts the valve core in real time according to the pressure feedback to ensure that the flow rates of the channels are consistent.

[0053] Scenario two: use of high-volatility solvents

[0054] In a high-temperature environment in summer, when acetone is used as an eluent, the thermoelectric cooling fin reduces the solvent temperature to 10°C, and the volatilization amount is reduced by 60%.

[0055] The wear-resistant shell surface is regularly wiped with alcohol, and the nano coating remains transparent and scratch-free, and the liquid level observation is clear.

[0056] 4. Summary of innovation advantages

[0057] Temperature control precision: thermoelectric cooling replaces ice packs to achieve a continuous low-temperature environment and avoid the impact of temperature fluctuations on elution efficiency.

[0058] Operation automation: closed-loop flow control reduces human intervention, improves experimental efficiency and result consistency.

[0059] Device durability: wear-resistant materials and sealing design extend the service life of the device and reduce laboratory maintenance costs.

[0060] High adaptability: flexible adjustment structure meets diverse experimental needs and is compatible with mainstream solid-phase extraction instrument models.

[0061] The above is only an embodiment of the present application, and does not limit the patent range of the present application, and any equivalent structure or equivalent process conversion using the content of the present application specification, or direct or indirect application in other related technical fields, are also included in the patent protection range of the present application.

Claims

1. A solid phase extraction liquid addition device, characterized by: The utility model relates to a kind of cooling device, including Fixing frame (100); Cooling cavity (500) is fixed on the fixing frame (100), and the cooling cavity (500) is equipped with thermoelectric refrigeration sheet and heat dissipation fin inside, and the cold end of the thermoelectric refrigeration sheet is towards cavity interior; Multi-channel liquid adding assembly, including at least two side-by-side liquid adding box (301), each liquid adding box (301) bottom is connected with liquid adding pipe (302), the liquid adding pipe (302) penetrates the bottom of the cooling cavity (500) and extends to outside, and automatic flow regulating valve (303) is equipped on the liquid adding pipe (302), and the automatic flow regulating valve (303) includes valve body, step motor driving valve body opening and closing, pressure sensor detecting pipeline pressure and microcontroller controlling step motor; Wear-resistant transparent shell (400), including box body that the cooling cavity (500) and liquid adding box (301) are wrapped, the box body is composed of polycarbonate base material outer coating nano-silica coating, and vertical scale is provided on the side wall of the liquid adding box (301); Height adjusting mechanism, including vertical fixing column (201) on fixing frame (100), horizontal support rod (204) and the first locking clamp (202) and second locking clamp (203) connecting vertical fixing column (201) with horizontal support rod (204), the first locking clamp (202) is fixed on vertical fixing column (201) by bolt, and the second locking clamp (203) is clamped horizontal support rod (204) by bolt.

2. The solid phase extraction liquid adding device according to claim 1, characterized in that: The thermoelectric refrigeration sheet is connected with external power supply, the microcontroller receives pressure sensor signal and feedback controls the rotating speed of step motor, to realize constant pressure liquid adding.

3. The solid phase extraction liquid addition device of claim 1, wherein: The box body top of the wear-resistant transparent shell (400) is equipped with sealing cover, and the sealing cover is opened with strip hole aligned with liquid adding box (301) top liquid adding port.

4. The solid phase extraction liquid addition device of claim 1, wherein: The cross section of the cooling cavity (500) is U-shaped, the liquid adding box (301) is embedded in U-shaped opening, and the cold end of the thermoelectric refrigeration sheet is close to the outer wall of the liquid adding box (301).

5. The solid phase extraction liquid addition device of claim 1, wherein: The first locking clamp (202) of the height adjusting mechanism is C-shaped clamp, and the inside is equipped with anti-skid rubber layer, and the second locking clamp (203) is U-shaped clamp, and both side walls are opened with through hole for horizontal support rod (204) to pass through.

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