Nucleic acid detection analyzer for biological medicine

Abstract

The utility model discloses a nucleic acid detection analyzer for biological medicine, which relates to the technical field of nucleic acid detection, solves the technical problem that multiple samples cannot be continuously detected in the existing technical scheme, and comprises a mainframe box, a sample conveying structure is mounted in the mainframe box, a laser emission structure is arranged in the mainframe box, and the laser emission structure is connected with the mainframe box. A fluorescent sensor, a fluorescent irradiator, a temperature adjusting structure and a fluorescent agent adding structure are arranged in the main machine box, and the sample conveying structure disclosed by the utility model can realize automatic conveying of samples by using the driving motor and the speed regulator; the working efficiency is improved; due to the design of the sample conveying belt, a plurality of samples can be continuously processed, and the consistency and the stability of detection are kept; the cooperative work of the laser emission structure and the fluorescence sensor improves the sensitivity and accuracy of nucleic acid detection, and target nucleic acid can be rapidly and reliably detected.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid detection technical field, concretely is a biological medicine nucleic acid detection analysis appearance. BACKGROUND

[0002] The biological medicine nucleic acid detection analysis instrument is a kind of efficient, accurate equipment, can carry out rapid detection and analysis to nucleic acid (such as DNA or RNA).Its main functions include sample processing, nucleic acid detection, data analysis and result management.Firstly, sample processing module can extract and purify nucleic acid sample, and it is pretreated to remove impurities and separate target nucleic acid.Then, detection module adopts real-time fluorescent PCR technology, detects specific nucleic acid sequence, and monitors amplification process in real time through fluorescent sensor.Data analysis module is quickly analyzed detection result and generates clear report through embedded computer or special software.In addition, storage and communication module can store detection data, and the result is uploaded to cloud or shared to external device through wireless communication function.Part of the equipment also supports multi-sample detection, portable design and remote monitoring function, further improves the practicability and flexibility of equipment.

[0003] The biological medicine nucleic acid detection analysis instrument has important significance in clinical diagnosis, public health, scientific research and drug development.Firstly, it provides fast and accurate technical support for disease diagnosis, especially in the detection of infectious diseases (such as new coronavirus, influenza virus) and genetic diseases, which can significantly shorten the detection time and improve the sensitivity.In public health events, this kind of equipment can detect samples on a large scale, help to find cases in time and control the spread of epidemic.In addition, in the field of scientific research, it provides an important tool for genetic engineering, molecular biology and drug development, helps researchers to analyze gene expression and pathogen mechanism, and promotes scientific progress.At the same time, this kind of equipment also supports personalized medicine, provides basis for precision treatment by detecting patients' genetic information.In economic and social aspects, it not only improves detection efficiency and reduces medical cost, but also reduces social and economic losses in public health events and protects people's health.Therefore, the biological medicine nucleic acid detection analysis instrument is the core equipment of modern biotechnology, and its wide application is making important contribution to human health and social development.

[0004] The existing technical solution has the technical problem that multiple samples cannot be continuously detected. UTILITY MODEL CONTENT

[0005] In view of the deficiencies of the prior art, the utility model provides a biological medicine nucleic acid detection analysis instrument, which solves the technical problem that the existing technical solution cannot continuously detect multiple samples.

[0006] In order to achieve the above object, the utility model discloses a biological medicine nucleic acid detection analyzer, including host case, the sample delivery structure is installed in the host case, the laser emission structure is arranged in the host case, the fluorescence sensor is arranged in the host case, the fluorescence illuminator is installed in the host case, the temperature adjusting structure is installed in the host case, the fluorescence agent adding structure is installed in the host case.

[0007] Preferably, the sample delivery structure includes a conveyor belt, a drive shaft and a driven shaft are rotatably installed in the host case, a driving sprocket is fixedly installed on the drive shaft, a driven sprocket is fixedly installed on the driven shaft, a transmission chain is engagedly connected to the driving sprocket and the driven sprocket, the conveyor belt is installed on the transmission chain, a test tube slot is provided on the conveyor belt, an operator inserts a new nucleic acid sample, the equipment drives the conveyor belt to the laser emission structure and the fluorescence illuminator and the fluorescence sensor below through the transmission chain, and the conveyor belt continuously rotates to convey out of the host case after detection.

[0008] Preferably, the sample delivery structure includes a driving motor, a speed regulator is fixedly installed in the host case, the driving motor is fixedly installed on the upper wall of the speed regulator, the input end of the speed regulator is fixedly connected to the driving end of the driving motor, a driving pulley is fixedly installed on the output end of the speed regulator, a driven pulley is fixedly installed on the lower end of the drive shaft, and a transmission belt is connected to the driving pulley and the driven pulley, which is used to drive the drive shaft to rotate, so that the conveyor belt can move on the transmission chain to complete the delivery of the nucleic acid sample.

[0009] Preferably, the fluorescence agent adding structure includes a liquid medicine storage box, the liquid medicine storage box is fixedly installed on the upper end of the host case, a liquid medicine injection pipe is fixedly installed on the side wall of the liquid medicine storage box, and the output end of the liquid medicine injection pipe is installed above the sample delivery structure for adding fluorescence agent liquid.

[0010] Preferably, a controller is fixedly installed on the upper wall of the host case, and a touch screen is fixedly installed on the side wall of the controller, which is used for operation and feedback of results.

[0011] Preferably, the temperature adjusting structure includes an electric heating resistance wire, and a temperature sensor is fixedly installed on the inner side wall of the conveyor belt, which is used to provide better temperature conditions for nucleic acid amplification and maintain a good analysis environment.

[0012] Advantages

[0013] The utility model provides a kind of nucleic acid detection analyzer for biological medicine, the utility model sample conveying structure by using driving motor and speed regulator, the automation of sample can be realized, manual operation is reduced, and work efficiency is improved;The design of sample conveying belt makes that multiple samples can be continuously handled, and the consistency and stability of detection are maintained;The synergistic work of laser emission structure and fluorescence sensor improves the sensitivity and accuracy of nucleic acid detection, and target nucleic acid can be detected quickly and reliably;Fluorescent agent adding structure ensures that fluorescent reagent is added in time during detection process, promotes the efficiency of nucleic acid amplification, and ensures the accuracy of detection result;Install electric heating resistance wire and temperature sensor to ensure that a stable temperature environment is maintained during the whole detection process.This is crucial for nucleic acid amplification reaction, and can significantly improve the success rate of experiment. BRIEF DESCRIPTION OF DRAWINGS

[0014] Fig. 1 It is a kind of nucleic acid detection analyzer for biological medicine of the utility model main view cross section structure schematic diagram.

[0015] Fig. 2 It is a kind of nucleic acid detection analyzer for biological medicine of the utility model planar transmission chain partial structure schematic diagram.

[0016] In the drawing: 1, mainframe box;2, laser emission structure;3, fluorescence sensor;4, fluorescent illuminator;5, conveying belt;6, driving shaft;7, driven shaft;8, driving sprocket;9, driven sprocket;10, transmission chain;11, test tube slot;12, driving motor;13, speed regulator;14, driving pulley;15, driven pulley;16, transmission belt;17, liquid medicine storage box;18, liquid medicine injection tube;19, controller;20, touch screen;21, electric heating resistance wire;22, temperature sensor; DETAILED DESCRIPTION

[0017] To further illustrate the technical means and effects adopted by the utility model to achieve the predetermined utility model purposes, the technical solutions in the embodiments of the utility model will be described clearly and completely in conjunction with the drawings in the embodiments of the utility model, and obviously, the described embodiments are only a part of the embodiments of the utility model, not all the embodiments, as follows.

[0018] Please refer to Figs. 1-2 The utility model provides a kind of technical scheme: a kind of nucleic acid detection analyzer for biological medicine, including mainframe box 1, the sample conveying structure is installed in the mainframe box 1, the laser emission structure 2 is provided in the mainframe box 1, the fluorescence sensor 3 is provided in the mainframe box 1, the fluorescent illuminator 4 is installed in the mainframe box 1, temperature adjusting structure is installed in the mainframe box 1, fluorescent agent adding structure is installed in the mainframe box 1.

[0019] In this embodiment, the sample delivery structure includes a conveyor belt 5. A drive shaft 6 and a driven shaft 7 are rotatably installed inside the main unit 1. An active sprocket 8 is fixedly installed on the drive shaft 6, and a driven sprocket 9 is fixedly installed on the driven shaft 7. A transmission chain 10 is meshed with the active sprocket 8 and the driven sprocket 9. The conveyor belt 5 is installed on the transmission chain 10. A test tube slot 11 is provided on the conveyor belt 5. When an operator inserts a new nucleic acid sample, the device drives the conveyor belt 5 through the transmission chain 10 to deliver the sample to the area below the laser emission structure 2, the fluorescence irradiator 4, and the fluorescence sensor 3. After the detection is completed, the sample continues to rotate and be delivered out of the main unit 1.

[0020] In this embodiment, the sample delivery structure includes a drive motor 12, a speed controller 13 is fixedly installed inside the main unit 1, the drive motor 12 is fixedly installed on the upper wall of the speed controller 13, the input end of the speed controller 13 is fixedly connected to the drive end of the drive motor 12, the output end of the speed controller 13 is fixedly installed with a drive pulley 14, the lower end of the drive shaft 6 is fixedly installed with a driven pulley 15, and a transmission belt 16 is connected to the drive pulley 14 and the driven pulley 15 to drive the drive shaft 6 to rotate, so that the conveyor belt 5 can move on the transmission chain 10 to complete the delivery of nucleic acid samples.

[0021] In this embodiment, the fluorescent agent addition structure includes a drug storage tank 17, which is fixedly installed on the upper part of the main unit 1. A drug injection tube 18 is fixedly installed on the side wall of the drug storage tank 17, and the output end of the drug injection tube 18 is installed above the sample delivery structure for adding fluorescent agent solution.

[0022] In this embodiment, a controller 19 is fixedly installed on the upper wall of the main unit 1, and a touch screen 20 is fixedly installed on the side wall of the controller 19. The controller 19 is operated and the results are fed back through the touch screen 20.

[0023] In this embodiment, the temperature regulation structure includes an electrothermal resistance wire 21, and a temperature sensor 22 is fixedly installed on the inner wall of the conveyor belt 5 to provide better temperature conditions for nucleic acid amplification and to maintain a good analytical environment.

[0024] Its detailed connection methods are well-known technologies in this field; such as Figs. 1-2 As shown, before use, ensure the equipment is functioning properly. Check the status of the power connection, sample delivery structure, fluorescence sensor 3, and laser emission structure 2.

[0025] Prepare nucleic acid extracts according to the requirements of the sample to be tested (such as blood, tissue or other biological samples), and ensure that the sample processing meets the experimental standards.

[0026] According to the experimental requirements, prepare the required fluorescent reagent and load it into the reagent storage box 17.

[0027] Load the nucleic acid sample into the test tube and place the test tube in the test tube slot 11 of the sample transport structure.

[0028] Select the appropriate detection parameters through the touch screen 20 controller 19. The operator can set parameters such as detection type, amplification reaction time, temperature, etc., depending on the sample type and experimental design.

[0029] Select the "Start Detection" option, and the motor 12 and speed regulator 13 will start working, the driving pulley 14 drives the driven pulley 15, so that the driving shaft 6 rotates, the driving shaft 6 drives the driving sprocket 8 and the driven sprocket 9 to rotate, and the sample transport structure moves the test tube. The system automatically moves the sample under the laser emission structure 2, the fluorescence illuminator 4 and the fluorescence sensor 3.

[0030] When the sample enters the laser emission area, the laser will excite the fluorescent reagent to produce a fluorescent signal. The fluorescence sensor 3 will capture these signals and monitor them in real time.

[0031] The temperature regulation structure ensures that the appropriate temperature is provided during the PCR amplification process, and after completion, the fluorescence sensor 3 transmits the captured signals to the controller 19 for data analysis.

[0032] After the detection is completed, the touch screen 20 will display the detection results, including positive or negative judgment, concentration value of the target nucleic acid, etc.

[0033] The operator can choose to save the detection result data and print the report through the external device, or upload the results to the laboratory information management system for subsequent analysis and record.

[0034] After use, remove the sample test tube, clean the equipment, and ensure that there is no residue in the reaction chamber and the sample transport structure.

[0035] Periodically check the working status of each component of the equipment, such as the driving motor 12, the fluorescence sensor 3 and the temperature control device, to ensure long-term stable operation of the equipment.

[0036] It should be noted that in this text, relational terms such as first and second are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between the entities or operations.

[0037] The above merely describes preferred embodiments of the present application, and is not intended to limit the present application in any form. Although the present application has been disclosed with preferred embodiments, it is not intended to limit the present application. Any person skilled in the art can make minor changes or modifications to the disclosed technical content, or make equivalent embodiments with equivalent changes, without departing from the technical solution of the present application. Any modification, equivalent change or modification of the above embodiments, which does not depart from the technical solution of the present application, is still within the scope of the technical solution of the present application.

Claims

1. A nucleic acid detection and analysis instrument for biomedicine, comprising a main unit (1), characterized in that, The main unit (1) is equipped with a sample delivery structure, a laser emission structure (2), a fluorescence sensor (3), a fluorescence irradiator (4), a temperature regulation structure, and a fluorescent agent addition structure.

2. The nucleic acid detection and analysis instrument for biomedicine according to claim 1, characterized in that... The sample conveying structure includes a conveyor belt (5), a drive shaft (6) and a driven shaft (7) are rotatably installed inside the main unit (1), a drive sprocket (8) is fixedly installed on the drive shaft (6), a driven sprocket (9) is fixedly installed on the driven shaft (7), a transmission chain (10) is meshed between the drive sprocket (8) and the driven sprocket (9), the conveyor belt (5) is installed on the transmission chain (10), and a test tube slot (11) is provided on the conveyor belt (5).

3. The nucleic acid detection and analysis instrument for biomedicine according to claim 2, characterized in that... The sample conveying structure includes a drive motor (12), a speed regulator (13) is fixedly installed inside the main unit (1), the drive motor (12) is fixedly installed on the upper wall of the speed regulator (13), the input end of the speed regulator (13) is fixedly connected to the drive end of the drive motor (12), the output end of the speed regulator (13) is fixedly installed with a drive pulley (14), the lower end of the drive shaft (6) is fixedly installed with a driven pulley (15), and a transmission belt (16) is connected to the drive pulley (14) and the driven pulley (15).

4. The nucleic acid detection and analysis instrument for biomedicine according to claim 1, characterized in that... The fluorescent agent addition structure includes a drug storage tank (17), which is fixedly installed on the upper end of the main unit (1). A drug injection tube (18) is fixedly installed on the side wall of the drug storage tank (17), and the output end of the drug injection tube (18) is installed above the sample delivery structure.

5. A nucleic acid detection and analysis instrument for biomedicine according to claim 1, characterized in that... A controller (19) is fixedly installed on the upper wall of the main unit (1), and a touch screen (20) is fixedly installed on the side wall of the controller (19).

6. A nucleic acid detection and analysis instrument for biomedicine according to claim 2, characterized in that... The temperature regulation structure includes an electric heating resistance wire (21), and a temperature sensor (22) is fixedly installed on the inner wall of the conveyor belt (5).

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