Flow type tube cell deposition rapid detection device
By installing a rapid detection device with a light source emitter and sensor at the bottom of the flow cytometer, the problem of insufficient cell precipitation in flow cytometry detection is solved, enabling real-time monitoring and accurate judgment, and avoiding experimental failure and sample waste.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-17
- Publication Date
- 2026-03-27
AI Technical Summary
During flow cytometry, researchers are unable to detect insufficient cell precipitation after centrifugation in a timely manner, leading to low experimental efficiency, sample waste, and experimental failure.
Design a rapid detection device for cell deposition in flow cytometry tubes. Utilize a light source emitter and a light intensity sensor to detect light intensity attenuation at the bottom of the flow cytometry tube. Combined with a control module and an alarm, monitor the cell deposition status in real time and promptly identify deficiencies.
This technology enables rapid and accurate detection of cell sedimentation at the bottom of flow cytometry tubes, avoiding experimental failures due to cell loss and improving experimental efficiency and sample protection.
Smart Images
Figure CN224051915U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell sample processing technical field, concretely relates to a flow pipe cell deposition rapid detection device. BACKGROUND
[0002] Flow cytometry is an indispensable detection technology in contemporary life science and medical research, and is widely used in immunology, oncology, cell biology and other fields. In the flow cytometry detection process, sample processing is the key link to determine the accuracy of the detection result, and the centrifugal washing step is particularly important. The centrifugal washing process usually includes centrifuging the treated cell sample to form a cell sediment, then discarding the supernatant, and finally resuspending the cells for machine detection.
[0003] However, the prior art has the following obvious shortcomings and deficiencies:
[0004] 1. Blind operation: researchers cannot directly judge whether there is enough cell sediment at the bottom of the flow tube after discarding the supernatant of the cell sample; since the cell sediment is often small in volume, it is difficult to accurately observe with the naked eye, especially when the cell concentration is low or the color is light.
[0005] 2. Delay in discovering problems: researchers can only discover the problem of insufficient cell number when the final machine detection is performed, which has consumed a lot of sample processing time and reagents.
[0006] 3. Multiple causes difficult to diagnose: cell loss may be caused by various reasons, such as inappropriate centrifugal parameters (too high or too low speed, insufficient time), poor cell activity, low cell concentration, improper operation, etc., but the prior art is difficult to discover and diagnose in time.
[0007] 4. Low experimental efficiency: due to the failure to discover the cell loss problem in time, researchers often need to repeat the entire experimental process, which greatly reduces the experimental efficiency and increases the research cost.
[0008] 5. Sample waste: for valuable clinical samples or rare cell populations, once the detection fails due to cell loss, it may not be possible to obtain the sample again for repeated experiments, causing irreparable loss.
[0009] These problems seriously affect the efficiency and reliability of flow cytometry detection, and an urgent need exists for a technical solution that can quickly detect the state of cell sediment. UTILITY MODEL CONTENT
[0010] The utility model aims at: in order to solve the problem that the researcher cannot find the problem of cell precipitation deficiency after centrifugation in time in flow cytometry detection process, provide a kind of flow tube cell deposition rapid detection device, whether the cell precipitation state at the bottom of flow tube meets the requirement can be quickly and accurately detected, to avoid the experimental failure caused by cell loss.
[0011] The utility model discloses a technical scheme as follows:
[0012] The utility model provides a kind of flow tube cell deposition rapid detection device, including base, frame body and top cover;The base is located at the bottom of the frame body, the top cover is located at the top of the frame body, the frame body is equipped with the slot for placing flow tube, the base is equipped with light source emitter, the light beam emitted by the light source emitter is directly injected from the bottom of flow tube to the direction of mouth portion, the top cover is equipped with light intensity sensor, the light intensity sensor corresponds with the light source emitter, for detecting the light intensity after passing through the bottom of flow tube, further include control module, the control module is connected with the light intensity sensor, to receive the data of light intensity sensor and calculate light attenuation ratio.
[0013] As a kind of feasible scheme of the utility model, further include alarm, the alarm is connected with the control module, for when detecting light attenuation amount is less than preset threshold value, alarm is sent, to remind researcher that cell precipitation can be insufficient.
[0014] As a kind of feasible scheme of the utility model, the base is equipped with power module, and the power module is used to provide electric energy for detection device.
[0015] As a kind of feasible scheme of the utility model, support plate and limit plate are sequentially arranged horizontally in the frame body from bottom to top, the support plate is supported at the bottom of flow tube, the limit plate is sleeved on the side of flow tube, and the support plate and the limit plate are placed vertically on the frame body.
[0016] As a kind of feasible scheme of the utility model, the frame body is equipped with support column, the top cover is rotatably connected with the support column, and the top cover can be switched between horizontal plane and vertical plane.
[0017] As a kind of feasible scheme of the utility model, the height of the support column relative to the upper end of the frame body is adjustable.
[0018] As a kind of feasible scheme of the utility model, the frame body is equipped with light-shielding sleeve, and the light-shielding sleeve is used to be sleeved on the section of flow tube extending out of the upper end of the frame body.
[0019] As a kind of feasible scheme of the utility model, the frame body is equipped with retaining sleeve, and the retaining sleeve is used to be sleeved on the section of flow tube located in the frame body.
[0020] As a kind of feasible scheme of the utility model, the light-shielding sleeve and the retaining sleeve are integrated structure.
[0021] As a kind of feasible scheme of the utility model, the base and the frame body bottom are detachably connected.
[0022] Compared with prior art, the utility model has the following advantages and beneficial effects:
[0023] The flow tube for completing centrifugation and discarding supernatant is inserted on the frame, the flow tube bottom is provided with light source emitter, the top cover is provided with light intensity sensor, and the light intensity sensor corresponds to the light source emitter, when cells are deposited at the flow tube bottom by centrifugal effect, a certain density of sediment layer is formed, the sediment layer will cause scattering and absorption of light entering the flow tube bottom, and then cause light intensity attenuation, the light intensity detected by the light intensity sensor will have obvious difference compared with reference light intensity (blank tube), the control module compares the difference to judge whether cell sedimentation reaches expected level, the detection device can quickly and accurately detect whether flow tube bottom cell sedimentation state meets requirements, and can effectively avoid experiment failure caused by cell loss. BRIEF DESCRIPTION OF DRAWINGS
[0024] In order to more clearly illustrate the technical scheme of the exemplary embodiments of the utility model, the following will briefly introduce the drawings needed to be used in the embodiments, and it should be understood that the following drawings only show some embodiments of the utility model, and therefore should not be regarded as limiting the scope, for ordinary skilled person in the art, other related drawings can also be obtained according to the drawings without creative labor.In the drawings:
[0025] Fig. 1 It is a flow tube cell deposition rapid detection device schematic view in the utility model;
[0026] Fig. 2 It is another flow tube cell deposition rapid detection device schematic view in the utility model;
[0027] Fig. 3 It is still another flow tube cell deposition rapid detection device schematic view in the utility model;
[0028] Fig. 4 It is the schematic view when the light-shielding sleeve and the retaining sleeve are integrated structure in the utility model.
[0029] Markings in the drawings and corresponding component names:
[0030] 1-base, 11-position ring, 2-frame, 21-supporting plate, 22-limiting plate, 23-strut, 24-rotating shaft, 25-seat hole sleeve, 3-top cover, 4-light source emitter, 5-light intensity sensor, 6-control module, 7-alarm, 8-power module, 9-shading sleeve, 91-retaining sleeve, L-flow pipe. DETAILED DESCRIPTION
[0031] In order to make the purpose, technical scheme and advantages of the utility model more clearly, the utility model is further described in detail below by combining with examples and drawings, the schematic implementation mode and the explanation thereof of the utility model are only used for explaining the utility model, and are not as the limitation of the utility model.
[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the specification herein is for describing the specific embodiments only and not intended to be limiting of the application; the terms "include" and "have" and any variations thereof used in the specification and the claims and the above description of the drawings are intended to cover the non-exclusive inclusion.
[0033] In the description of the embodiments of the present application, the technical terms "first", "second", etc. are only used to distinguish different objects, and cannot be understood as indicating or implying relative importance or implicitly indicating the number, specific order or primary and secondary relationship of the indicated technical features.
[0034] Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearance of the phrase in various places in the specification does not necessarily all refer to the same embodiment, nor is it necessarily independent or alternative embodiments to each other. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.
[0035] In the description of the embodiments of the present application, the term "and / or" is only a description of the association relationship of the associated objects, which means that there can be three relationships, for example, A and / or B, which means that there are three cases of A, A and B, and B. In addition, the character " / " in this paper generally represents a "or" relationship between the front and rear associated objects.
[0036] In the embodiments of the present application, the same reference signs represent the same parts, and for brevity, detailed description of the same parts is omitted in different embodiments. It should be understood that the thickness, length and width of various components in the embodiments of the present application shown in the drawings, and the overall thickness, length and width of the integrated device are only exemplary and should not constitute any limitation on the present application.
[0037] In the description of the embodiments of the present application, the term "a plurality of" refers to two or more (including two), and similarly, "a plurality of groups" refers to two or more groups (including two groups), and "a plurality of pieces" refers to two or more pieces (including two pieces), unless otherwise explicitly specified and limited.
[0038] In the description of the embodiments of the present application, the technical terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the embodiments of the present application and simplifying the description, and does not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the embodiments of the present application.
[0039] In the description of the embodiments of the present application, unless otherwise explicitly specified and limited, the technical terms "mounting", "connecting", "connecting", "fixing" and the like should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the embodiments of the present application can be understood according to the specific circumstances.
[0040] Please refer to Figs. 1 to 4 The flow tube L cell deposition rapid detection device provided in the embodiments of the present application comprises a base 1, a frame body 2 and a top cover 3; the base 1 is arranged at the bottom of the frame body 2, the top cover 3 is arranged at the top of the frame body 2, the frame body 2 is provided with a slot for placing a flow tube L, the base 1 is provided with a light source emitter 4, the light beam emitted by the light source emitter 4 is directly incident from the bottom to the mouth of the flow tube L, the top cover 3 is provided with a light intensity sensor 5, the light intensity sensor 5 corresponds to the light source emitter 4, and is used for detecting the light intensity after passing through the bottom of the flow tube L, and further comprises a control module 6, the control module 6 is connected with the light intensity sensor 5 to receive the data of the light intensity sensor 5 and calculate the light intensity attenuation ratio.
[0041] The rack body 2 in the embodiment is a cuboid shell structure, the flow tube L is inserted on the rack body 2, the base 1 is connected to the lower end of the rack body 2, the top cover 3 is connected to the upper end of the rack body 2, the light source emitter 4 is arranged on the base 1 at a position opposite to the bottom of the flow tube L, and the light intensity sensor 5 is arranged on the top cover 3 at a position opposite to the mouth of the flow tube L. The light beam emitted by the light source emitter 4 is emitted from the bottom of the flow tube L, then emitted from the mouth of the flow tube L and detected by the light intensity sensor 5, and the state of cell precipitation is judged by detecting the light intensity attenuation after passing through the bottom of the flow tube L.
[0042] In use, the flow tube L, which is completed centrifugation and the supernatant is discarded, is inserted on the rack body 2, when the cells are deposited at the bottom of the flow tube L by centrifugation, a certain density of the sediment layer is formed, the sediment layer will cause scattering and absorption of the light emitted into the bottom of the flow tube L, and then cause the light intensity attenuation, the light intensity detected by the light intensity sensor 5 will have obvious difference compared with the reference light intensity (blank tube), the control module 6 compares the difference to judge whether the cell precipitation reaches the expected level; the detection device can quickly and accurately detect whether the cell precipitation state at the bottom of the flow tube L meets the requirements, and can effectively avoid experimental failure caused by cell loss.
[0043] It should be noted that the light source emitter 4 can emit light beams of a specific wavelength range, preferably green light or red light and the like which have good penetration on cells. For the specific structure and principle of the light source emitter 4, reference can be made to a laser pen emitting green light or red light, which will not be described here. The flow tube L slot on the rack body 2 can have one or more, when there are multiple flow tube L slots, the light source emitter 4 and the light intensity sensor 5 and the like can be correspondingly provided.
[0044] According to some embodiments of the present application, an alarm 7 is further included, which is connected with the control module 6, used to issue an alarm when the light intensity attenuation is less than a preset threshold, to remind the researchers that the cell precipitation may be insufficient.
[0045] When the light intensity attenuation is less than a preset threshold (such as 30%), an alarm is issued. It should be noted that the alarm 7 can be an indicator light. In use, the reference light intensity is calibrated by the blank tube first, then the light intensity attenuation ratio of the flow tube L is detected in real time, and when the attenuation ratio is less than 30%, the indicator light at the corresponding position can change from green to red.
[0046] According to some embodiments of the present application, the base 1 is provided with a power module 8, which is used to provide electric energy for the detection device. The power module 8 can be a storage battery and the like.
[0047] According to some embodiments of the present application, the support plate 21 and the limiting plate 22 are arranged in the rack body 2 from bottom to top in sequence. The support plate 21 supports the bottom of the flow tube L, and the limiting plate 22 is sleeved on the side of the flow tube L. The support plate 21 and the limiting plate 22 together enable the flow tube L to be vertically placed on the rack body 2.
[0048] The support plate 21 is provided with a light beam incident through hole, and the inner diameter of the light beam incident through hole is slightly smaller than the outer diameter of the flow tube L. In addition, the end of the light beam incident through hole in contact with the flow tube L is provided with a hole opening chamfer, so as to support and limit the bottom of the flow tube L. The limiting plate 22 can have one or more than one through hole, and the through hole is provided on the limiting plate 22. The inner diameter of the through hole is greater than the outer diameter of the flow tube L, so as to enable the flow tube L to be inserted.
[0049] According to some embodiments of the present application, the rack body 2 is provided with a support column 23, and the top cover 3 is rotationally connected with the support column 23 through a rotating shaft 24. The top cover 3 can be switched between a horizontal plane and a vertical plane. By using the above scheme, the top cover 3 is flipped to a horizontal state during detection, so that the light intensity sensor 5 on the top cover 3 is just located at the mouth of the flow tube L, so as to detect the light intensity attenuation after passing through the flow tube L. After detection, the top cover 3 is flipped to a vertical state, so as to facilitate the taking out of the flow tube L.
[0050] According to some embodiments of the present application, the height of the support column 23 relative to the upper end of the rack body 2 is adjustable. Specifically, the lower part of the support column 23 is inserted into the seat hole sleeve 25 on the upper end of the rack body 2. Thus, the support column 23 can be lifted along the seat hole sleeve 25, so as to adjust the height of the top cover 3 relative to the upper end of the rack body 2. Thus, the flow tube L within a certain length range can be adapted.
[0051] It should be noted that the connection cables between the related components in the drawings of the present application are not shown, and according to the scheme that the height of the top cover 3 relative to the upper end of the rack body 2 is adjustable, the related connection cables need to be reserved with a certain length requirement, so as to avoid excessive pulling of the related connection cables when the height of the top cover 3 is adjusted.
[0052] According to some embodiments of the present application, the rack body 2 is provided with a light shielding sleeve 9, and the light shielding sleeve 9 is used to sleeve a section of the flow tube L extending out of the upper end of the rack body 2. Since the upper part of the flow tube L exceeds the upper end of the rack body 2 after the flow tube L is placed on the rack body 2, the flow tube L is convenient to take out after detection, but the external light will also affect the detection of the light intensity sensor 5. Therefore, the present application sleeves a light shielding sleeve 9 on the section of the flow tube L. The light shielding sleeve 9 can shield the external light, and can also support the lower side of the top cover 3.
[0053] According to some embodiments of the present application, the frame 2 is provided with a retaining sleeve 91, which is used to cover a section of the flow tube L located in the frame 2. By providing the retaining sleeve 91 in the frame 2, the flow tube L is inserted into the retaining sleeve 91, so that by adjusting the inner hole diameter of the retaining sleeve 91, the flow tube L within a certain diameter range can be adapted.
[0054] According to some embodiments of the present application, the light shielding sleeve 9 and the retaining sleeve 91 are an integral structure. By designing as an integral structure, the light shielding sleeve 9 and the retaining sleeve 91 can be replaced at the same time. For example, when the above structure is prefabricated, various sizes A and sizes B can be formed to meet the use of flow tubes L within a certain specification size range. After detection, the top cover 3 is opened, the light shielding sleeve 9 and the retaining sleeve 91 are taken out as a whole, and then the flow tube L is taken out from the frame 2. The light shielding sleeve 9 and the retaining sleeve 91 described above can be made of black shock-absorbing cotton material, of course, other light-proof materials can also be used.
[0055] According to some embodiments of the present application, the base 1 and the bottom of the frame 2 are detachably connected. Specifically, the base 1 and the bottom of the frame 2 are fixedly connected by bolts. The base 1 is provided with a positioning ring 11, which is positioned with the bottom of the frame 2, so as to facilitate the alignment of the light beam emitted by the light source emitter 4 with the bottom of the flow tube L.
[0056] The flow tube L cell deposition rapid detection device in the present application has at least the following advantages:
[0057] 1. Real-time monitoring: the cell sedimentation state can be detected immediately after the centrifugal supernatant step, without waiting for the detection to find the problem.
[0058] 2. Objective quantification: the objective data of light intensity attenuation instead of subjective visual judgment improves the accuracy and consistency of judgment.
[0059] 3. Improve efficiency: the problem of insufficient cell sedimentation can be found in time, avoiding entering the subsequent invalid operation, saving experimental time and reagent consumption.
[0060] 4. Diagnosis assistance: according to the light intensity attenuation characteristics, the possible reasons for insufficient cell sedimentation (such as insufficient centrifugation, low cell concentration, etc.) can be preliminarily judged.
[0061] 5. Protecting valuable samples: for valuable or rare cell samples, it can avoid the irreparable loss caused by operation errors.
[0062] 6. Standardized operation: providing a standardized quality control point for flow cytometry detection experiment, reducing the experimental variation caused by the experience difference of operators.
[0063] The above specific embodiments explain the purpose, technical scheme and beneficial effects of the present application in further detail, and it should be understood that the above description is only a specific embodiment of the present application and is not used to limit the protection scope of the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims
1. A flow tube cell sedimentation rapid detection device, characterized in that, Including base, frame and top cover; the base is equipped in the frame bottom, the top cover is equipped in the frame top, the frame is equipped with the slot for placing flow pipe, the base is equipped with light source emitter, the light beam that the light source emitter emits is opposite from flow pipe bottom to mouth direction and injects, the top cover is equipped with light intensity sensor, the light intensity sensor corresponds with the light source emitter, is used for detecting the light intensity after passing through flow pipe bottom, still include control module, the control module is linked with the light intensity sensor, receives the data of light intensity sensor and calculates light intensity attenuation ratio.
2. The flow tube cell deposition rapid detection device of claim 1, wherein, Still include alarm, the alarm is linked with the control module, is used for sending alarm when detecting that light intensity attenuation amount is less than the preset threshold, to remind the researcher that cell precipitation can be insufficient.
3. The flow tube cell deposition rapid detection device of claim 1, wherein, The base is equipped with power module, and the power module is used to provide electric energy for the detection device.
4. The flow tube cell deposition rapid detection device of claim 1, wherein, The support plate and the limiting plate are sequentially arranged in the frame from bottom to top, the support plate supports the flow pipe, the limiting plate is sleeved on the side of the flow pipe, and the support plate and the limiting plate are used for vertically placing the flow pipe on the frame.
5. The flow tube cell deposition rapid detection device of claim 1, wherein, The frame is equipped with a support column, the top cover is rotationally connected with the support column, and the top cover can be switched between a horizontal plane and a vertical plane.
6. The flow cytometric cell deposition rapid detection device of claim 5, wherein, The height of the support column relative to the upper end of the frame is adjustable.
7. The flow tube cell deposition rapid detection device of claim 1, wherein, The frame is equipped with a light shielding sleeve, and the light shielding sleeve is used for sleeving a section of the flow pipe extending out of the upper end of the frame.
8. The flow tube cell deposition rapid detection device of claim 7, wherein, The frame is equipped with a retaining sleeve, and the retaining sleeve is used for sleeving a section of the flow pipe in the frame.
9. The flow tube cell deposition rapid detection device of claim 8, wherein, The light shielding sleeve and the retaining sleeve are an integral structure.
10. The flow tube cell deposition rapid detection device of claim 1, wherein, The base is detachably connected with the bottom of the frame.