Multi-needle automatic sample application device for SNP (Single Nucleotide Polymorphism) chip
By designing a multi-needle automatic spotting device, the SNP chip is automatically spotted using X, Y, Z, and W directional drive arms and gas cylinders. This solves the problems of cumbersome and inefficient traditional spotting processes, achieving efficient and safe automated spotting and avoiding cross-contamination.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-18
- Publication Date
- 2026-03-27
AI Technical Summary
The traditional SNP chip spotting process is cumbersome, inefficient, requires a lot of manual intervention, poses a risk of cross-contamination, and is noisy.
Design a multi-needle automatic sampling device, including a platform, a moving component, a sample carrying component, and a sampling component. It adopts X, Y, Z, and W-axis drive arms and gas cylinders to achieve automated sample aspiration, sampling, and cleaning. The sampling needles are staggered to avoid cross-contamination.
It improves the efficiency of SNP chip spotting, reduces manual intervention, lowers noise, avoids cross-contamination, and achieves efficient, accurate, and safe automated spotting.
Smart Images
Figure CN224052220U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to biological point sampling equipment technical field, specifically related to a kind of multi-needle automatic point sampling device for SNP chip. BACKGROUND
[0002] Biochip is through microtechnique, according to the principle of specific interaction between molecules, the discontinuous analysis process in the field of life science is integrated on the surface of silicon chip or glass chip microbiochemical analysis system, to realize the accurate, fast, large information detection of cell, protein, gene and other biological components.
[0003] Traditional 7-needle SNP chip point sampling process has many processes, such as: 4 times of sample suction, 5 times of cleaning, cross use of point sampling needle, waiting for drying after point sampling, nitrogen cylinder for providing pressure, large noise, cross contamination risk due to cross use of point sampling needle, low efficiency, and large amount of manual participation in the whole process. UTILITY MODEL CONTENT
[0004] The utility model aims at providing a kind of multi-needle automatic point sampling device for SNP chip, can automatically complete SNP chip point sampling, degree of automation is high, so that SNP chip point sampling processing can be efficient, accurate, safe, stable, without cross contamination risk.
[0005] To solve the above technical problems, the utility model adopts the following scheme:
[0006] A kind of multi-needle automatic point sampling device for SNP chip, including platform, mobile assembly, sample loading assembly and point sampling assembly are arranged above the platform, Y direction driving arm and Z direction driving arm in the mobile assembly are fixedly arranged on the top of platform both sides rack driving point sampling assembly to move point sampling, the sample loading assembly is connected with X direction driving arm and W direction driving arm in mobile assembly, drives sample loading assembly to move to the lower side of point sampling assembly, the point sampling assembly includes needle disc seat and point sampling needle in needle disc seat, the needle disc seat is fixedly connected with the bottom of Z direction driving arm.
[0007] Further, the top of Y direction driving arm is provided with gas cylinder, the gas cylinder is communicated with the top end of point sampling needle by pipeline and makes point sampling needle sample suction or point sampling.
[0008] Further, the number of point sampling needle is 28, and each horizontal row is 7, and the point sampling needle of adjacent two rows is staggered.
[0009] Further, the sample loading assembly includes chip tray one and chip tray two, the chip tray one is connected with X direction driving arm, and the chip tray two is communicated with W direction driving arm.
[0010] Further, the X-direction driving arm and the W-direction driving arm drive the chip tray one and the chip tray two to slide relative to each other.
[0011] Further, the chip tray one and the chip tray two are provided with a plurality of chips, and each row of point sample needles in the needle disc seat corresponds to a same row of microwells of four chips.
[0012] Further, the X-direction driving arm and the W-direction driving arm are arranged in parallel, the Y-direction driving arm and the Z-direction driving arm are arranged in intersection, and the X-direction driving arm, the Y-direction driving arm, the Z-direction driving arm and the W-direction driving arm are all ball screw modules and are driven by servo motors.
[0013] Further, the side of the rack is provided with a sample pool, a vacuum pool, a cleaning pool and an ultrasonic pool, and the sample pool, the vacuum pool and the cleaning pool are provided with a plurality of cavities consistent with the number and distribution of the point sample needles.
[0014] Further, the gas cylinder drives the point sample needle to suck liquid when the gas cylinder is under negative pressure, and the gas cylinder drives the point sample needle to empty liquid when the gas cylinder is under negative pressure.
[0015] Further, the ultrasonic pool is provided with an ultrasonic generator.
[0016] The utility model has the beneficial effect that:
[0017] The point sample needle in the needle disc seat is provided with one hole in each row, corresponds to 1-28 holes in the SNP chip, a plurality of chips are used for adding samples of the same sample, and the sample adding rate is obviously improved. The X, Y, Z and W direction driving walls complete the whole point sample movement, the automation degree is high, the cleaning degree is high, and the cross contamination risk is avoided. BRIEF DESCRIPTION OF DRAWINGS
[0018] Figure 1 It is a structural schematic view of the utility model;
[0019] Figure 2 It is a top view structural schematic view of the utility model;
[0020] Figure 3 It is a partial enlarged structural schematic view of the utility model Figure 1 when point sampling;
[0021] Figure 4 It is a hole position top view of the SNP chip.
[0022] The drawings show that: 1 is a platform, 10 is a rack, 2 is a point sample assembly, 20 is a needle disc seat, 21 is a point sample needle, 22 is a gas cylinder, 3 is a moving assembly, 31 is an X-direction driving arm, 32 is a Y-direction driving arm, 33 is a Z-direction driving arm, 34 is a W-direction driving arm, 4 is a sample loading assembly, 40 is a chip tray one, 41 is a chip tray two, 5 is a sample pool, 6 is a cleaning pool, 7 is a vacuum pool, and 8 is an ultrasonic pool. DETAILED DESCRIPTION
[0023] The utility model will be further explained in detail in combination with the embodiments and drawings, but the implementation mode of the utility model is not limited to this.
[0024] In the description of the utility model, it needs to be explained that the orientation or position relation indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "longitudinal", "lateral", "horizontal", "inner", "outer", "front", "rear", "top", "bottom" and the like is based on the orientation or position relation shown in the drawings or the orientation or position relation that the utility model product is usually placed in, which is only for the convenience of describing the utility model and simplifying the description, and cannot be understood as a limitation on the utility model that the indicated device or element must have a specific orientation, be constructed and operated in a specific orientation, therefore, it cannot be understood as a limitation on the utility model.
[0025] In the description of the utility model, it also needs to be explained that, unless otherwise explicitly specified and limited, the terms "set", "open", "install", "connect", "connect" should be understood in a broad sense, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected, it can be mechanically connected, or electrically connected, it can be directly connected, or indirectly connected through an intermediate medium, it can be the communication inside two elements, the above-mentioned terms in the utility model can be understood according to the specific meaning in the utility model by the ordinary skill in the art.
[0026] Embodiment
[0027] The embodiment of the utility model is a kind of multi-needle automatic sample point device for SNP chip, including platform 1, the upper portion of platform 1 is provided with moving assembly 3, sample loading assembly 4 and sample point assembly 2, Y direction driving arm 32 and Z direction driving arm 33 in moving assembly 3 are fixedly arranged on the top of platform 1 both sides rack 10 drive point sample assembly 2 to move point sample, sample loading assembly 4 is connected with X direction driving arm 31 and W direction driving arm 34 in moving assembly 3 to drive sample loading assembly 4 to move to the lower portion of sample point assembly 2, sample point assembly 2 includes needle disc seat 20 and sample point needle 21 located in needle disc seat 20, needle disc seat 20 is fixedly connected with the bottom of Z direction driving arm 33.
[0028] Reference Figure 1 , the application is provided with moving assembly 3, sample loading assembly 4 and sample point assembly 2 on the upper portion of platform 1, moving assembly 3 drives the overall movement of sample point assembly 2 on one hand, carries out sample suction and sample point and cleaning, drives sample loading assembly 4 to move to the lower portion of sample point assembly 2 on the other hand, at this time, sample point assembly 2 will be moved to the upper portion of sample loading assembly 4, sample loading assembly 4 is added to the chip in, the number of one sample is 28 needles, and the same sample is added to multiple chips, which significantly improves the sample adding rate.
[0029] Meanwhile, the Y-direction driving arm 32 drives the pin disc seat 20 and the pin 21 in the sample dispensing assembly 2 to move laterally to the desired position, and then the Z-direction driving arm 33 drives the pin 21 in the sample dispensing assembly 2 to move downward to perform sample suction and dispensing. The X-direction driving arm 31 and the W-direction driving arm 34 move the sample loading device to the lower side of the sample dispensing assembly 2, and after each sample dispensing, the sample loading device is continuously moved so that the micro-holes of the chip in the sample loading assembly 4 are always in the same vertical axis with the sample dispensing pin.
[0030] In some preferred embodiments, a gas cylinder 22 is arranged on the top of the Y-direction driving arm 32, which is communicated with the top end of the sample dispensing pin 21 through a pipeline to enable the sample dispensing pin 21 to perform sample suction or dispensing.
[0031] The gas cylinder 22 is filled with compressed air, which provides pressure through the pipeline to enable the sample dispensing pin 21 to perform sample suction or dispensing. Nitrogen gas cylinder 22 is not required to provide pressure, which reduces the cost of sample dispensing. When the gas cylinder 22 is under negative pressure, the sample dispensing pin 21 is driven to suck liquid, and when the gas cylinder 22 is under negative pressure, the sample dispensing pin 21 is driven to empty the liquid. The sample dispensing pin 21 is automatically completed under the driving of the external control system, including sample suction, cleaning, sample discharge and sample dispensing. The external control system belongs to the prior art, which is not described here.
[0032] In some preferred embodiments, the number of sample dispensing pins 21 is 28, and each horizontal row has 7 sample dispensing pins 21. The sample dispensing pins 21 in adjacent two rows are arranged in staggered manner. During sample dispensing, 28 sample dispensing pins 21 are dispensed at one time, and each row has one hole in staggered manner, which corresponds to 1-28 holes in the chip, respectively. Sample suction and dispensing are not required to be performed multiple times, which accelerates the sample dispensing speed.
[0033] Meanwhile, the sample loading assembly 4 includes a chip tray one 40 and a chip tray two 41. The chip tray one 40 is connected with the X-direction driving arm 31, and the chip tray two 41 is communicated with the W-direction driving arm 34. The X-direction driving arm 31 and the W-direction driving arm 34 respectively drive the chip tray one 40 and the chip tray two 41 to slide relative to each other. A plurality of SNP chips are respectively arranged in the chip tray one 40 and the chip tray two 41.
[0034] During sample dispensing, the X-direction driving arm 31 drives the chip tray one 40 to move linearly to the lower side of the sample dispensing pin 21, so that each sample dispensing pin 21 corresponds to the micro-holes in the chip. Then, the Z-direction driving arm 33 and the gas cylinder 22 are driven to enable the sample dispensing pin 21 to perform sample dispensing. After the chip tray one 40 is completed with sample dispensing, the X-direction driving arm 31 drives the chip tray one 40 to move out of the sample dispensing pin 21, and the W-direction driving arm 34 drives the chip tray two 41 to move linearly to the lower side of the sample dispensing pin 21 to perform sample dispensing.
[0035] It should be noted that the chip tray one 40 and the chip tray two 41 are provided with a plurality of chips, and each row of the point sample needle 21 in the needle disc seat 20 corresponds to the same row of microwells of four chips. That is, during the sampling of the point sample needle 21, the microwells of the same row of four chips are sampled at one time. Since the point sample needles 21 of the adjacent two rows are staggered by one hole, the microwells in each chip will also be staggered for sampling during each sampling process. Referring to Figure 3 and Figure 4 In each row, the adjacent point sample needles 21 are spaced apart by three hole positions of the SNP chip, that is, in Figure 4 , the hole positions sampled by the outermost row of point sample needles 21 in the needle disc seat 20 are the hole positions 1-7, and the subsequent next row of sampling hole positions are 8-14, 15-21, and 22-28. After one-time sampling, repeated sampling is performed, so that the first row of hole positions in the four chips at this time are all sampled, and the sampling of the next row of chips is repeatedly performed.
[0036] In some preferred embodiments, the X-direction driving arm 31 and the W-direction driving arm 34 are arranged in parallel, the Y-direction driving arm 32 and the Z-direction driving arm 33 are arranged in intersection, and all are ball screw modules driven by servo motors, which are prior art and will not be described here.
[0037] In some preferred embodiments, the sample pool 5, the vacuum pool 7, the cleaning pool 6, and the ultrasonic pool 8 are arranged on the side of the rack 10, and the sample pool 5, the vacuum pool 7, and the cleaning pool 6 are provided with a plurality of cavities consistent with the number and distribution of the point sample needles 21. Referring to Figure 2 , the Y-direction driving arm 32 and the Z-direction driving arm 33 mainly control the movement of the point sample needles 21 to the sample pool 5, the vacuum pool 7, the cleaning pool 6, and the ultrasonic pool 8, and perform corresponding work according to the required program, and start the gas cylinder 22 to perform liquid suction or discharge; meanwhile, the cavities of each adjacent row in the sample pool 5, the vacuum pool 7, and the cleaning pool 6 are staggered.
[0038] In some preferred embodiments, the ultrasonic pool 8 is provided with an ultrasonic generator. The arrangement of the ultrasonic generator is beneficial to improve the cleaning rate and effect of the point sample needles 21.
[0039] The multi-needle automatic sampling device of the present application is connected with an external controller. When in use, the power switch of the device is turned on, the start button is pressed, the device is started, the X-direction driving arm 31, the Y-direction driving arm 32, the Z-direction driving arm 33, and the W-direction driving arm 34 are reset, and after the reset is completed, the machine is in standby state.
[0040] Click on the display screen on the start point sample key, Y to drive arm 32, Z to drive arm 33 control 28 needle needle disc seat 20 operation to sample pool 5 position to empty, run to the cleaning pool 6 position for cleaning, peristaltic pump continuously pumping pure water to the cleaning pool 6, flush 28 needle sample needle 21 outer wall, cylinder 22 into negative pressure, control 28 needle sample needle 21 from the cleaning pool 6 in the cleaning of pure water, cylinder 22 into positive pressure, the suction of pure water, so repeatedly several times, in order to achieve 28 needle sample needle 21 cleaning purposes. In the cleaning pool 6 after cleaning is completed, Y to drive arm 32, Z to drive arm 33 control 28 needle needle disc seat 20 operation to ultrasonic pool 8 position, ultrasonic cleaner, use ultrasonic wave to clean the sample needle 21 outer wall again, while cylinder 22 into negative pressure, control 28 needle sample needle 21 from the ultrasonic pool 8 in the suction of pure water, cylinder 22 into positive pressure again, the suction of pure water, so repeatedly several times, in order to achieve 28 needle sample needle 21 cleaning purposes. Ultrasonic cleaning is completed Y to drive arm 32, Z to drive arm 33 control 28 needle needle disc seat 20 operation to sample pool 5 position again to empty, run to the vacuum pool 7 in the extraction of straight empty, 28 needle sample needle 21 outer wall on the residual liquid suction clean.
[0041] Y to drive arm 32, Z to drive arm 33 control 28 needle needle disc seat 20 operation to sample pool 5 position, wait, after adding new reagent in sample pool 5 position, click start sample again, Z to drive arm 33 control 28 needle needle disc seat 20 to drop into the sample pool 5, suction reagent, reagent suction is completed, Y to drive arm 32, Z to drive arm 33 control 28 needle needle disc seat 20 operation to vacuum pool 7 position again to vacuum, 28 needle sample needle 21 outer wall on the residual liquid suction clean. Run to the sample position to pre spray, pre spray after X to drive arm 31 chip tray one 40 operation to 28 needle needle disc seat 20 below, when the sample needle 21 and SNP chip hole end to open the corresponding micro valve, and the reagent is sprayed into the SNP chip hole, X to drive arm 31 on the SNP chip complete sample, W to drive arm 34 again chip tray two 41 operation to 28 needle needle disc seat 20 below for sample.
[0042] W to drive arm 34 on the chip tray two 41 sample complete, the machine waiting for replacement X to drive arm 31, W to drive arm 34 SNP chip tray one 40 and chip tray two 41, click "start sample" button again, so repeat the sample.
[0043] After all the sample points are finished, the Y-direction driving arm 32 and the Z-direction driving arm 33 control the 28-needle needle disc seat 20 to run to the sample pool 5 position to empty the unused reagent, and then run to the cleaning pool 6 position for cleaning. The peristaltic pump continuously pumps pure water to the cleaning pool 6 to flush the outer wall of the 28-needle sample point needle 21. The gas cylinder 22 is switched to negative pressure, the 28-needle sample point needle 21 is controlled to suck pure water from the cleaning pool 6, the gas cylinder 22 is switched to positive pressure to discharge the sucked pure water, and the above process is repeated for multiple times to achieve the purpose of cleaning the 28-needle sample point needle 21. After the cleaning pool 6 is cleaned, the Y-direction driving arm 32 and the Z-direction driving arm 33 control the 28-needle needle disc seat 20 to run to the ultrasonic pool 8 position, the ultrasonic cleaner is opened, the outer wall of the needle is cleaned again by ultrasonic waves, the gas cylinder 22 is switched to negative pressure, the 28-needle sample point needle 21 is controlled to suck pure water from the ultrasonic pool 8, the gas cylinder 22 is switched to positive pressure again to discharge the sucked pure water, and the above process is repeated for multiple times to achieve the purpose of cleaning the 28-needle sample point needle 21. After the ultrasonic cleaning is completed, the Y-direction driving arm 32 and the Z-direction driving arm 33 control the 28-needle needle disc seat 20 to run to the sample pool 5 position to empty again, and then run to the vacuum pool 7 to suck the residual liquid on the outer wall of the 28-needle sample point needle 21.
[0044] The above process is repeated to perform cyclic sample point.
[0045] The above is only a preferred embodiment of the present application, and does not limit the present application in any form. According to the technical essence of the present application, any simple modification, equivalent replacement and improvement of the above embodiment within the spirit and principles of the present application are still within the protection scope of the technical scheme of the present application.
Claims
1. A multi-needle automatic spotting device for SNP chips, characterized by, The application relates to a platform (1) provided with a moving assembly (3), a sample loading assembly (4) and a sample spotting assembly (2) above the platform (1), Y-direction driving arms (32) and Z-direction driving arms (33) in the moving assembly (3) are fixed to the top of rack (10) on both sides of the platform (1) to drive the sample spotting assembly (2) to move, the sample loading assembly (4) is connected with X-direction driving arms (31) and W-direction driving arms (34) in the moving assembly (3) to drive the sample loading assembly (4) to move below the sample spotting assembly (2), the sample spotting assembly (2) comprises a needle disc seat (20) and sample spotting needles (21) in the needle disc seat (20), and the needle disc seat (20) is fixed to the bottom of the Z-direction driving arms (33).
2. The multi-needle automatic spotting device for SNP chip according to claim 1, wherein, The top of the Y-direction driving arms (32) is provided with a gas cylinder (22), the gas cylinder (22) is communicated with the top end of the sample spotting needles (21) through pipelines and drives the sample spotting needles (21) to suck sample or spot sample.
3. The multi-needle automatic spotting device for SNP chip according to claim 1, wherein, The number of the sample spotting needles (21) is 28, and each horizontal row has seven sample spotting needles (21); the sample spotting needles (21) in adjacent two rows are arranged in a staggered mode.
4. The multi-needle automatic spotting device for SNP chip according to claim 2, wherein, The sample loading assembly (4) comprises a chip tray one (40) and a chip tray two (41), the chip tray one (40) is connected with the X-direction driving arms (31), and the chip tray two (41) is communicated with the W-direction driving arms (34).
5. The multi-needle automatic spotting device for SNP chip according to claim 4, wherein, The X-direction driving arms (31) and the W-direction driving arms (34) drive the chip tray one (40) and the chip tray two (41) to slide relative to each other.
6. The multi-needle automatic spotting device for SNP chip according to claim 4, wherein, The chip tray one (40) and the chip tray two (41) are provided with a plurality of chips, and each row of the sample spotting needles (21) in the needle disc seat (20) corresponds to the same row of micropores of four chips.
7. The multi-needle automatic spotting device for SNP chip according to claim 4, wherein, The X-direction driving arms (31) and the W-direction driving arms (34) are arranged in parallel, the Y-direction driving arms (32) and the Z-direction driving arms (33) are arranged in intersection, and the X-direction driving arms (31), the W-direction driving arms (34), the Y-direction driving arms (32) and the Z-direction driving arms (33) are all ball screw modules and are driven by servo motors.
8. The multi-needle automatic spotting device for SNP chip according to claim 4, wherein, The rack (10) is provided with a sample pool (5), a vacuum pool (7), a cleaning pool (6) and an ultrasonic pool (8) on the side, the sample pool (5), the vacuum pool (7) and the cleaning pool (6) are provided with a plurality of cavities consistent with the number and distribution of the sample spotting needles (21).
9. The multi-needle automatic spotting device for SNP chip according to claim 4, wherein, The gas cylinder (22) drives the sample spotting needles (21) to suck liquid when the gas cylinder (22) is under negative pressure, and the gas cylinder (22) drives the sample spotting needles (21) to empty liquid when the gas cylinder (22) is under negative pressure.
10. The multi-needle automatic spotting device for SNP chip according to claim 8, wherein, An ultrasonic generator is installed in the ultrasonic pool (8).