Stem cell conditioned medium filtering device

Abstract

The utility model discloses a stem cell conditioned medium filter device which comprises a filter device shell, a filter tank is arranged in the filter device shell, a square sleeve is arranged on the lower side in the filter tank, a coarse-mesh filter screen is fixedly arranged in the square sleeve, and a filter screen is arranged in the coarse-mesh filter screen. A plurality of fine-hole filter screens fixed in the square sleeve are fixedly arranged below the coarse-hole filter screen; a sealing plate fixed in the square sleeve is arranged above the coarse-mesh filter screen, and a first stepping motor is fixedly mounted in the sealing plate. According to the utility model, the situation that the filter holes in the filter screen are blocked by cell debris, dead cells or metabolic wastes in the stem cell conditioned culture medium and even penetrate through the filter holes due to relatively intense impact generated between the filter screen and the stem cell conditioned culture medium when the filter screen is used for filtering the stem cell conditioned culture medium is prevented; through the technical scheme, the filtering effect of the equipment is improved.

Description

Technical Field

[0001] This utility model relates to the field of stem cell technology, specifically a stem cell conditioned medium filtration device. Background Technology

[0002] Stem cell conditioned medium is a culture medium used to support the growth and self-renewal capacity of stem cells. It contains essential nutrients, growth factors and regulatory factors to provide the environmental conditions required for stem cell growth and differentiation. Stem cells are a type of pluripotent cell with self-replication capacity. Under certain conditions, they can differentiate into various functional cells. According to the developmental stage of stem cells, they are divided into embryonic stem cells and adult stem cells.

[0003] In addition to beneficial components such as cytokines, stem cell conditioned medium also contains harmful substances such as cell growth and metabolism waste, dead cells, and cell debris. Therefore, in order to improve the purity of stem cells before use, a specific filtration device is needed to filter them. The device used to filter these stem cells can be called a stem cell conditioned medium filtration device.

[0004] However, in the current stem cell conditioned medium filtration device, the stem cell conditioned medium is directly poured onto the filter screen for filtration. During this process, cell debris, dead cells, and metabolic waste in the stem cell conditioned medium may become stuck in the filter screen or even pass through the filter screen due to the impact force between the stem cell conditioned medium and the filter screen, ultimately affecting the filtration effect of the device. Therefore, this does not meet the existing requirements. To address this, we have proposed a stem cell conditioned medium filtration device. Utility Model Content

[0005] The purpose of this invention is to provide a stem cell conditioned medium filtration device to solve the problems mentioned in the background art, where the stem cell conditioned medium is directly poured onto the filter screen for filtration. During this process, cell debris, dead cells, and metabolic waste in the stem cell conditioned medium may become stuck in the filter pores of the filter screen or even pass through the filter pores due to the impact force between the stem cell conditioned medium and the filter screen, ultimately affecting the filtration effect of the device.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a stem cell conditioned medium filtration device, comprising a filter device shell, a filter groove inside the filter device shell, a square sleeve inside the filter groove, a coarse-pore filter screen fixed inside the square sleeve, and a plurality of fine-pore filter screens fixed inside the square sleeve below the coarse-pore filter screen.

[0007] Above the coarse-pore filter screen is a sealing plate fixed inside the square sleeve. A first stepper motor is fixedly installed inside the sealing plate. The output shaft of the first stepper motor is connected to a first threaded rod through a coupling. A metal column is provided on the outside of the first threaded rod through a threaded structure. A lifting sealing plate is fixed on the upper end face of the metal column. A multi-turn through-hole sealing head is fixed on the lower end face of the lifting sealing plate. A through hole is provided on the outside of the through-hole sealing head on the outer surface of the sealing plate.

[0008] A second stepper motor is fixedly installed inside the outer shell of the filter device on one side of the square sleeve. The output shaft of the second stepper motor is connected to a second threaded rod through a coupling. The second threaded rod is connected to the front end of the square sleeve through a threaded structure, and the rear end face of the square sleeve is slidably connected to the inner wall of the filter tank.

[0009] Preferably, a control panel is fixedly installed on one side of the outer surface of the filter device housing, and both the first stepper motor and the second stepper motor are electrically connected to the control panel.

[0010] Preferably, one side of the control panel is provided with a discharge head that is fixedly installed on the outer surface of the filter device housing, and the discharge head communicates with the interior of the filter tank.

[0011] Preferably, a guide rod is provided on both sides of the first threaded rod and fixed to the lower end face of the lifting sealing plate, and the guide rod is movably inserted into the interior of the sealing plate.

[0012] Preferably, the outer surface of the square sleeve is in contact with the inner wall of the filter tank, and the square sleeve and the filter tank are slidably connected.

[0013] Preferably, an adding head is provided above the square sleeve and fixed to the upper end face of the filter device housing, and the interior of the adding head is in communication with the interior of the filter tank.

[0014] Preferably, a protective cover is provided below the square sleeve on the lower end face of the filter device housing, and the filter device housing and the protective cover are fixed together by screws.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] 1. This utility model allows stem cell conditioned medium to be poured directly into the filter tank via the addition head when the device is needed. After the stem cell conditioned medium in the filter tank has settled and stabilized, the second stepper motor drives the fine-pore and coarse-pore filters inside the filter tank to slowly move upward, thereby completing the filtration of the stem cell conditioned medium. The above technical solution can prevent the strong impact between the fine-pore and coarse-pore filters when filtering the stem cell conditioned medium, which could cause cell debris, dead cells, or metabolic waste inside the stem cell conditioned medium to get stuck in the filter holes of the fine-pore or coarse-pore filters or even pass through the filter holes, thus improving the filtration effect of the device.

[0017] 2. In this invention, the stem cell conditioned medium, just poured into the filter tank, cannot come into contact with the fine-pore and coarse-pore filter screens fixed to the inner wall of the square sleeve due to the isolation provided by the lifting sealing plate and the sealing plate. The isolation provided by the lifting sealing plate and the sealing plate can prevent the stem cell conditioned medium from colliding with the fine-pore and coarse-pore filter screens when adding the stem cell conditioned medium, thus preventing cell debris, dead cells, or metabolic waste from getting stuck in or even passing through the filter pores of the fine-pore or coarse-pore filter screens. The above technical solution further improves the filtration effect of the equipment. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a front view of the entire utility model;

[0020] Figure 3 This utility model Figure 2 Enlarged view of the structure at point A in the middle;

[0021] Figure 4 This utility model Figure 3 Enlarged view of the structure at point B.

[0022] In the diagram: 1. Filter housing; 2. Control panel; 3. Discharge head; 4. Adding head; 5. Filter tank; 6. Square sleeve; 7. Coarse-pore filter screen; 8. Fine-pore filter screen; 9. Sealing plate; 10. First stepper motor; 11. First threaded rod; 12. Lifting sealing plate; 13. Guide rod; 14. Through hole; 15. Through hole sealing head; 16. Metal column; 17. Second stepper motor; 18. Second threaded rod. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0024] The discharge head 3 (model GID), coarse-pore filter screen 7 (model GZxcI145) and second stepper motor 17 (model DM542) mentioned in this utility model can be obtained from the market or through private customization.

[0025] Please see Figures 1 to 4 An embodiment of this utility model is provided: a stem cell conditioned culture medium filtration device, including a filter device shell 1, a filter groove 5 inside the filter device shell 1, a square sleeve 6 inside the filter groove 5, a coarse-pore filter screen 7 fixed inside the square sleeve 6, and a plurality of fine-pore filter screens 8 fixed inside the square sleeve 6 below the coarse-pore filter screen 7.

[0026] A sealing plate 9 is fixed inside the square sleeve 6 above the coarse mesh filter 7. A first stepper motor 10 is fixedly installed inside the sealing plate 9. The output shaft of the first stepper motor 10 is connected to a first threaded rod 11 through a coupling. A metal column 16 is connected to the first threaded rod 11 through a threaded structure on the outside of the first threaded rod 11. A lifting sealing plate 12 is fixedly installed on the upper end face of the metal column 16. A multi-turn through-hole sealing head 15 is fixedly installed on the lower end face of the lifting sealing plate 12. A through hole 14 is located on the outer surface of the sealing plate 9 on the outside of the through-hole sealing head 15.

[0027] A second stepper motor 17 is fixedly installed inside the housing of the filter device housing 1 on one side of the square sleeve 6. The output shaft of the second stepper motor 17 is connected to a second threaded rod 18 through a coupling. The second threaded rod 18 is connected to the front end of the square sleeve 6 through a threaded structure, and the rear end face of the square sleeve 6 is slidably connected to the inner wall of the filter tank 5.

[0028] A control panel 2 is fixedly installed on one side of the outer surface of the filter housing 1, and the first stepper motor 10 and the second stepper motor 17 are electrically connected to the control panel 2; the first stepper motor 10 and the second stepper motor 17 can be controlled through the control panel 2.

[0029] The square sleeve 6 has an addition head 4 fixed to the upper end face of the filter device housing 1, and the interior of the addition head 4 is connected to the interior of the filter tank 5. When the equipment is to be used, stem cell conditioned medium is added into the filter tank 5 through the addition head 4. The stem cell conditioned medium added into the filter tank 5 will be isolated from the coarse pore filter 7 and the fine pore filter 8 by the sealing plate 9 fixed above the coarse pore filter 7 and the lifting sealing plate 12 that is attached to the upper end face of the sealing plate 9. The above technical solution can prevent the stem cell conditioned medium from colliding with the fine pore filter 8 and the coarse pore filter 7 when adding stem cell conditioned medium, so that cell debris, dead cells or metabolic waste located inside the stem cell conditioned medium will get stuck on the filter holes of the fine pore filter 8 or the coarse pore filter 7 or even pass through the filter holes. This structure can improve the filtration effect of the equipment.

[0030] Both sides of the first threaded rod 11 are provided with a guide rod 13 fixed to the lower end face of the lifting sealing plate 12, and the guide rod 13 is movably inserted into the interior of the sealing plate 9; when the stem cell condition culture medium added to the filter tank 5 is statically stabilized, the first step motor 10 is started, and the first step motor 10 can drive the first threaded rod 11 connected to it to rotate.

[0031] Since the lower end face of the lifting sealing plate 12, which is fixed to the metal column 16, is equipped with a guide rod 13 that is movable and inserted into the sealing plate 9, neither the lifting sealing plate 12 nor the metal column 16 can rotate. When the first threaded rod 11 rotates, the metal column 16, which cannot rotate on its own, will move up and down under the drive of the threaded structure. At this time, the metal column 16 moves upward. During the upward movement of the metal column 16, the lifting sealing plate 12, which is fixed to it, and the through hole sealing head 15, which is fixed to the lower end face of the lifting sealing plate 12, will leave from the inside of the through hole 14 located on the outer surface of the sealing plate 9. After the through hole sealing head 15 completely leaves from the inside of the through hole 14, the stem cell conditioned culture medium located inside the filter tank 5 will pass through the through hole 14 and contact the coarse pore filter screen 7 under the action of gravity.

[0032] When the stem cell conditioned medium comes into contact with the coarse-pore filter 7, the second stepper motor 17 is activated. The second stepper motor 17 drives the connected second threaded rod 18 to rotate. Because the rear end face of the square sleeve 6, which is connected to the second threaded rod 18 via a threaded structure, is slidably connected to the filter tank 5, the filter tank 5 cannot rotate on its own. When the second threaded rod 18 rotates, the square sleeve 6, which is threaded to it but cannot rotate on its own, will move up and down under the drive of the threaded structure. At this time, the square sleeve 6 moves slowly upwards. During the upward movement, the coarse-pore filter inside the square sleeve 6 is fixed... The coarse-pore filter 7 and the fine-pore filter 8 will move upward together. The filtration of the stem cell conditioned medium can be completed by the gradually moving coarse-pore filter 7 and the fine-pore filter 8. The above technical solution can prevent the strong impact between the fine-pore filter 8 and the coarse-pore filter 7 when filtering the stem cell conditioned medium, so that cell debris, dead cells or metabolic waste inside the stem cell conditioned medium can get stuck on the filter holes of the fine-pore filter 8 or the coarse-pore filter 7 or even pass through the filter holes, thereby improving the filtration effect of the equipment.

[0033] A discharge head 3 is fixedly installed on the outer surface of the filter housing 1 on one side of the control panel 2, and the discharge head 3 is connected to the inside of the filter tank 5; after the filtration of the stem cell conditioned medium is completed, the filtered stem cell conditioned medium can be discharged through the discharge head 3.

[0034] After the filtered stem cell conditioned culture medium has been completely drained, add disinfectant to the inside of filter tank 5 and shake the equipment vigorously for one minute. Then pour out the disinfectant to complete the disinfection and cleaning of the equipment.

[0035] The outer surface of the square sleeve 6 is in contact with the inner wall of the filter tank 5, and the square sleeve 6 and the filter tank 5 are slidably connected; this structure can prevent the stem cell conditioned culture medium that has just been added to the filter tank 5 from flowing through the gap between the filter tank 5 and the square sleeve 6 to the bottom of the fine filter screen 8.

[0036] The square sleeve 6 is provided with a protective cover plate located on the lower end face of the filter device housing 1, and the filter device housing 1 and the protective cover plate are fixed together by screws; the protective cover plate can protect the internal structure of the filter device housing 1, and when the internal structure of the filter device housing 1 fails, the protective cover plate can be removed to repair the internal structure of the filter device housing 1.

[0037] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A stem cell conditioned medium filtration device comprising a filtration device housing (1), characterized in that: The inside of the filter device shell (1) is provided with a filter groove (5), the lower side of the inside of the filter groove (5) is provided with a square sleeve (6), the inside of the square sleeve (6) is fixedly provided with a coarse mesh filter screen (7), the lower side of the coarse mesh filter screen (7) is fixedly provided with a plurality of fine mesh filter screens (8) fixed in the inside of the square sleeve (6); The upper side of the coarse mesh filter screen (7) is provided with a sealing plate (9) fixed in the inside of the square sleeve (6), the inside of the sealing plate (9) is fixedly installed with a first stepping motor (10), the output shaft of the first stepping motor (10) is connected with a first threaded rod (11) through a shaft coupling, the outer side of the first threaded rod (11) is provided with a metal column (16) connected thereto through a threaded structure, the upper end surface of the metal column (16) is fixedly provided with a lifting sealing plate (12), the lower end surface of the lifting sealing plate (12) is fixedly provided with a plurality of through-hole sealing heads (15), the outer side of the through-hole sealing head (15) is provided with a through-hole (14) located on the outer surface of the sealing plate (9); One side of the square sleeve (6) is provided with a second stepping motor (17) fixedly installed in the inside of the filter device shell (1), the output shaft of the second stepping motor (17) is connected with a second threaded rod (18) through a shaft coupling, the second threaded rod (18) is connected between the front end of the square sleeve (6) through a threaded structure, and the rear end surface of the square sleeve (6) is slidably connected with the inner wall of the filter groove (5).

2. The stem cell conditioned medium filtration device of claim 1, wherein: One side of the outer surface of the filter device shell (1) is fixedly installed with a control panel (2), and the first stepping motor (10) and the second stepping motor (17) are electrically connected with the control panel (2).

3. The stem cell conditioned medium filtration device of claim 2, wherein: One side of the control panel (2) is provided with a discharge head (3) fixedly installed on the outer surface of the filter device shell (1), and the discharge head (3) is communicated with the inside of the filter groove (5).

4. The stem cell conditioned medium filtration device of claim 1, wherein: Both sides of the first threaded rod (11) are provided with a guide rod (13) fixed to the lower end surface of the lifting sealing plate (12), and the guide rod (13) is movably inserted into the inside of the sealing plate (9).

5. The stem cell conditioned medium filtration device of claim 1, wherein: The outer surface of the square sleeve (6) is attached to the inner wall of the filter groove (5), and the square sleeve (6) is slidably connected with the filter groove (5).

6. The stem cell conditioned medium filtration device of claim 1, wherein: The upper side of the square sleeve (6) is provided with an adding head (4) fixed to the upper end surface of the filter device shell (1), and the inside of the adding head (4) is communicated with the inside of the filter groove (5).

7. The stem cell conditioned medium filtration device of claim 1, wherein: The lower side of the square sleeve (6) is provided with a protective cover plate located on the lower end surface of the filter device shell (1), and the filter device shell (1) and the protective cover plate are fixedly connected through screws.

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