Quantitative liquid suction and drainage device

Abstract

The utility model provides a quantitative liquid suction and drainage device which comprises a pipette connector, the pipette connector comprises a connecting main body, a cavity part is arranged in the connecting main body, a rotatable rotating core is arranged in the cavity part, a plurality of through quantitative cavities are formed in the rotating core, a slidable piston is arranged in each quantitative cavity, and the volumes of the quantitative cavities are different. The upper end and the lower end of the cavity part are respectively provided with a first air guide hole and a second air guide hole which are eccentric to the central axis of the rotating core, the central axes of the first air guide hole and the second air guide hole are aligned, the lower end of the connecting main body is further provided with a connecting nozzle, the second air guide hole is communicated with the connecting nozzle, and the connecting nozzle is used for being connected with a transfer pipette in a sleeved mode. The problem that a gas path system is polluted during automatic pipetting is solved.

Description

Technical Field

[0001] This utility model relates to the field of glass slide preparation, and in particular to a quantitative liquid suction and discharge device. Background Technology

[0002] In liquid-based slide preparation technology, cells or tissues are collected using a brush or other collection tools, and the samples are stored in a sampling bottle containing a preservation solution. The bottle is then sealed and transported to the slide preparation station, where necessary reagents are added to the cell samples. The samples are then transferred to the slide preparation chamber of a centrifuge for centrifugation and are made into glass slides.

[0003] In automated slide preparation equipment, due to the large workload and high precision requirements, traditional manual operation is no longer sufficient for quantitative reagent addition and sample transfer. Therefore, automated pipettes are typically used for reagent addition and sample transfer. Traditional pipettes usually use negative pressure for liquid aspiration. After aspiration, the negative pressure gas path valve switches to a closed state, maintaining negative pressure in the pipette. When liquid needs to be expelled, the pipette switches to positive pressure to expel the liquid. However, because the gas path system is directly connected to the pipette, contaminants on the surface of the reagent or sample can enter the gas path system during negative pressure aspiration, contaminating the valve body and tubing. Even after replacing the pipette, the positive pressure gas can still carry contaminants from the tubing and valve body into other reagents or samples, causing cross-contamination. Utility Model Content

[0004] This invention provides a quantitative liquid suction and discharge device, which solves the problem of contamination of the gas path system during automatic liquid transfer.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows: a quantitative aspiration and dispensing device, including a pipette connector, the pipette connector including a connecting body, a cavity portion inside the connecting body, a rotatable rotating core inside the cavity portion, a plurality of through-flow quantitative chambers on the rotating core, a slidable piston inside each quantitative chamber, each quantitative chamber having a different volume, each quantitative chamber being eccentrically arranged around the central axis of the rotating core, a first air guide hole and a second air guide hole being eccentrically arranged around the central axis of the rotating core at the upper and lower ends of the cavity portion, the central axes of the first air guide hole and the second air guide hole being aligned, a connecting nozzle being provided at the lower end of the connecting body, the second air guide hole communicating with the connecting nozzle, the connecting nozzle being used to connect with a pipette.

[0006] In the preferred embodiment, the lower end of the connecting body is open, and the lower end of the connecting body is provided with a centrally through bottom cover. The bottom cover is provided with a U-shaped inner liner cover, with one end of the opening of the U-shaped inner liner cover facing the bottom cover. The second air guide hole is provided on the U-shaped inner liner cover.

[0007] In a preferred embodiment, the outer wall of the rotating core is provided with a plurality of first magnetic tiles along the circumference, with adjacent first magnetic tiles having opposite magnetic poles. The outer wall of the connecting body is fitted with a rotatable rotating sleeve, and the inner wall of the rotating sleeve is provided with a plurality of second magnetic tiles along the circumference, with adjacent second magnetic tiles having opposite magnetic poles.

[0008] In a preferred embodiment, the bottom end of the cap is provided with a connecting nozzle, which has multiple cylindrical sleeve parts arranged in a stepped manner, and the cylindrical sleeve parts are used to connect with a pipette.

[0009] In a preferred embodiment, a vertically movable lower extension rod connecting plate is also included. The pipette connector is located on the lower extension rod connecting plate. A flange is provided on the outer edge of the upper end of the pipette. A pipette unloading rack is provided on one side of the pipette connector. A lever is provided at the lower end of the unloading rack, and the end of the lever is attached to the outer wall of the connecting nozzle.

[0010] In a preferred embodiment, a third linear motion mechanism is also included. The third linear motion mechanism includes a base frame, on which a lifting frame plate that can move up and down is provided. A lower extension rod is provided on the lifting frame plate, and the lower end of the lower extension rod is connected to a lower extension rod connecting plate. A lower extension rod guide seat is provided on the base frame, and the lower extension rod guide seat is slidably sleeved with the lower extension rod. A lifting plate guide rod is provided at the upper end of the pipe unloading frame, and a guide rod guide seat is also provided on the base frame. The lifting plate guide rod is slidably sleeved with the guide rod guide seat. A spring balancer is provided at the upper end of the base frame, and the spring balancer suspends the upper end of the lifting plate guide rod.

[0011] In a preferred embodiment, the lower end of the pipette rack has a hollow section with a hinge shaft at the hollow section. One end of the lever is hinged to the hinge shaft, and the lever has a first countersunk hole. A baffle is provided on the side of the hinge shaft away from the pipette connector, and a second countersunk hole is provided inside the baffle. A spring is also provided, with one end of the spring inserted into the first countersunk hole and abutting against the bottom of the first countersunk hole, and the other end of the spring inserted into the second countersunk hole and abutting against the bottom of the second countersunk hole.

[0012] The beneficial effects of this invention are as follows: A piston separates the gas path system and the pipette, preventing contaminating gases or suspended droplets from entering the gas path system and causing cross-contamination during subsequent pipetting; multiple quantitative chambers of different volumes are used, with each chamber volume matching the required reagent volume of the pipette, allowing selective aspiration of a set amount of liquid for addition; a detachable pipette is connected via a pipette connector, which features cylindrical fittings of various diameters to accommodate different pipette sizes; a lever can swing and remains firmly against the outer wall of the connector, automatically removing the pipette after pipetting and replacing it with a new, clean pipette, thus preventing sample cross-contamination. Attached Figure Description

[0013] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0014] Figure 1 This is a schematic diagram of a pipette and its Cartesian three-dimensional movement mechanism.

[0015] Figure 2 This is a schematic diagram of the up-and-down movement mechanism of a pipette.

[0016] Figure 3 This is a partial view of the pipette connector.

[0017] Figure 4 This is a schematic diagram of the pipette connector and the pipette unloading mechanism.

[0018] Figure 5 This is a cross-sectional view of the connector.

[0019] Figure 6 This is a diagram showing the non-working position of the paddle shifter.

[0020] Figure 7 This is a schematic diagram of the lowest working position of the paddle shifter.

[0021] Figure 8 This is a cross-sectional view of a quantitative pipette connector.

[0022] Figure 9 This is an exploded view of a quantitative pipette connector.

[0023] Figure 10 This is a diagram of the internal rotating core structure of a pipette connector.

[0024] In the figure: First linear movement mechanism 1; transverse frame 101; base plate 102; first guide rail slider device 103; first drive motor 104; first lead screw 105; first lead screw nut 106; second linear movement mechanism 2; base frame 201; cantilever frame 202; second guide rail slider device 203; second drive motor 204; synchronous belt mechanism 205; synchronous belt clamping block 206; third linear movement mechanism 3; lifting frame plate 301; third drive motor 302; second lead screw 303; second lead screw nut 304; third guide rail slider device 305; lower extension rod 4; lower extension rod connecting plate 401; lower extension rod guide seat 402; positioning hole 403; locking screw 404; pipette connector 5; connecting nozzle 501 ; Cylindrical sleeve part 502; First air guide hole 503; Positioning protrusion 504; Annular groove part 505; Notch part 506; Air connector 507; Connecting body 508; Rotating core 509; Metering chamber 510; Piston 511; U-shaped inner liner cover 512; Second air guide hole 513; Bottom cover 514; First magnetic tile 515; Rotating sleeve 516; Second magnetic tile 517; Sealing plate 518; Limiting locking ring 519; Cavity part 520; Unloading pipe rack 6; Paddle 601; Lifting plate guide rod 602; Guide rod guide seat 603; Slot part 604; Hollowed-out part 605; Hinge shaft 606; First countersunk hole 607; Spring 608; Baffle 609; Second countersunk hole 610; Pipette 7; Flange edge 701; Spring balancer 8. Detailed Implementation

[0025] like Figure 1-10 A quantitative aspiration and dispensing device includes a pipette connector 5. The pipette connector 5 includes a connecting body 508, a cavity 520 inside the connecting body 508, a rotatable rotating core 509 inside the cavity 520, and multiple through-holes 510 on the rotating core 509. Each 510 has a slidable piston 511 inside it. The volume of each 510 is different, and each 510 is eccentrically arranged around the central axis of the rotating core 509. The upper and lower ends of the cavity 520 are each provided with a first air guide hole 503 and a second air guide hole 513, which are eccentrically arranged around the central axis of the rotating core 509. The central axes of the first air guide hole 503 and the second air guide hole 513 are aligned. The lower end of the connecting body 508 is also provided with a connecting nozzle 501. The second air guide hole 513 communicates with the connecting nozzle 501, and the connecting nozzle 501 is used to connect with a pipette 7.

[0026] The metering chambers 510 at both ends of the rotating core 509 are equipped with sealing plates 518.

[0027] Rotating the core 509 allows switching between the metering chamber 510 aligned with the first air guide hole 503 and the second air guide hole 513, thus switching the metering chamber 510 that ultimately connects to the connecting nozzle 501.

[0028] Initially, piston 511 is located at the lower end of metering chamber 510. When drawing liquid, pipette connector 5, along with pipette 7, is inserted into the reagent bottle. The first vent 503 is connected to negative pressure, and piston 511 moves upward under the action of negative pressure, drawing up the reagent. Since the volume of metering chamber 510 is fixed, the reagent drawn up by pipette 7 is also quantitative.

[0029] When it is necessary to remove the reagent, the first gas inlet 503 switches to positive pressure gas, pushing the piston 511 to move downward and squeezing out the liquid drawn from the pipette 7.

[0030] Each piston 511 has a sealing ring on its outer wall that fits against the inner wall of the metering chamber 510, which can separate the two sides of the space and prevent the upper space from being contaminated by the gas in the lower space.

[0031] In the preferred embodiment, the lower end of the connecting body 508 is open, and the lower end of the connecting body 508 is provided with a centrally through bottom cover 514. The bottom cover 514 is provided with a U-shaped inner liner 512, one end of the opening of the U-shaped inner liner 512 faces the bottom cover 514, and the second air vent 513 is provided on the U-shaped inner liner 512.

[0032] The U-shaped inner liner 512 is threadedly connected to the bottom cover 514. Then, the second air vent 513 is aligned with the first air vent 503, and the bottom cover 514 is locked to the lower opening of the connecting body 508 by screws.

[0033] In a preferred embodiment, the outer wall of the rotating core 509 is provided with a plurality of first magnetic tiles 515 along the circumferential direction, and the magnetic poles of adjacent first magnetic tiles 515 are opposite. The outer wall of the connecting body 508 is fitted with a rotatable rotating sleeve 516, and the inner wall of the rotating sleeve 516 is provided with a plurality of second magnetic tiles 517 along the circumferential direction, and the magnetic poles of adjacent second magnetic tiles 517 are opposite.

[0034] The lower end of the rotating sleeve 516 abuts against the flange on the outer wall of the connecting body 508, and the upper end is provided with a limiting locking ring 519 that is threadedly connected to the connecting body 508 to prevent the second magnetic tile 517 from falling out. The clamping wall between the rotating core 509 and the rotating sleeve 516 is relatively thin and does not isolate the magnetic force. When the rotating sleeve 516 rotates, it can drive the internal rotating core 509 to rotate, which can adjust and align the metering cavity 510 with the first air guide hole 503 and the second air guide hole 513. Then, the limiting locking ring 519 is locked to tighten and fix the rotating sleeve 516.

[0035] In a preferred embodiment, the bottom cover 514 is provided with a connecting nozzle 501 at its lower end. The connecting nozzle 501 is provided with a plurality of cylindrical sleeve portions 502 arranged in a stepped manner. The cylindrical sleeve portions 502 are used to connect with the pipette 7.

[0036] The diameter of the lower cylindrical sleeve portion 502 is slightly smaller than that of the upper cylindrical sleeve portion 502, so the connecting nozzle 501 can be fitted with various pipettes 7 of different diameters.

[0037] When aspirating, pipettes 7 of different diameters can aspirate reagents of different volumes. If there are three cylindrical sleeves 502 of different diameters, then the rotating core 509 is equipped with three quantitative chambers 510 of different volumes. Before use, the corresponding quantitative chamber 510 can be manually adjusted or switched by the motor synchronous belt mechanism.

[0038] In a preferred embodiment, a vertically movable lower extension rod connecting plate 401 is also included. The pipette connector 5 is disposed on the lower extension rod connecting plate 401. A flange edge 701 is provided on the upper outer edge of the pipette 7. A pipette unloading rack 6 is provided on one side of the pipette connector 5. A lever 601 is provided at the lower end of the pipette unloading rack 6. The end of the lever 601 is attached to the outer wall of the connecting nozzle 501.

[0039] The pipette 7 adopts a customized structure with an outwardly protruding flange 701 on the outer edge of the upper port. When the lower extension rod connecting plate 401 moves the pipette connector 5 upward, the paddle 601 has a slot 604. The slot 604 abuts against the flange 701 and scrapes the pipette 7 off.

[0040] In a preferred embodiment, a third linear motion mechanism 3 is also included. The third linear motion mechanism 3 includes a base frame 201, on which a vertically movable lifting frame plate 301 is provided. A lower extension rod 4 is provided on the lifting frame plate 301, and the lower end of the lower extension rod 4 is connected to a lower extension rod connecting plate 401. A lower extension rod guide seat 402 is provided on the base frame 201, and the lower extension rod guide seat 402 is slidably sleeved with the lower extension rod 4. A lifting plate guide rod 602 is provided at the upper end of the pipe unloading frame 6. A guide rod guide seat 603 is also provided on the base frame 201, and the lifting plate guide rod 602 is slidably sleeved with the guide rod guide seat 603. A spring balancer 8 is provided at the upper end of the base frame 201, and the spring balancer 8 suspends the upper end of the lifting plate guide rod 602.

[0041] When the pipette connector 5 moves downward, its lower end abuts against the lever 601, causing the pipette rack 6 to descend. The connector 501 is inserted into the pipette 7 on the lower pipette rack. Since the pipette 7 is made of plastic, it has a certain degree of elasticity. The beveled structure at the lower end of the cylindrical sleeve 502 is inserted into the pipette 7 first, and the pipette opening expands slightly and fits onto the cylindrical sleeve 502, which can form a seal and has a certain clamping force.

[0042] Subsequently, the lifting platform 301 moves upward, causing the pipette 7 to detach from the pipe rack. The spring balancer 8 adjusts the tension to be slightly greater than the total weight of the unloading rack 6 and related structures. Under the action of the spring balancer 8, the unloading rack 6 moves upward and resets along with the pipette connector 5. When the upper end of the unloading rack 6 is stopped by the guide rod guide seat 603, it no longer moves upward. At this time, the pipette connector 5 continues to move upward, and the lever 601 can scrape off the pipette 7.

[0043] In a preferred embodiment, the lower end of the tube unloading rack 6 is provided with a hollow portion 605, and a hinge shaft 606 is provided at the hollow portion 605. One end of the lever 601 is hinged to the hinge shaft 606. The lever 601 is provided with a first countersunk hole 607. A baffle 609 is provided on the side of the hinge shaft 606 away from the pipette connector 5. A second countersunk hole 610 is provided in the baffle 609. A spring 608 is also provided. One end of the spring 608 is inserted into the first countersunk hole 607 and abuts against the bottom end of the first countersunk hole 607. The other end of the spring 608 is inserted into the second countersunk hole 610 and abuts against the bottom end of the second countersunk hole 610.

[0044] Under the elastic force of spring 608, the end of the paddle 601 is always tightly pressed against the outer wall of the connector 501. No matter what diameter pipette 7 is fitted onto the connector 501, the slot 604 can abut against the cylindrical sleeve 502 of the next level. When the connector 501 moves upward relative to the paddle 601, the pipette 7 is scraped off.

[0045] The lower extension rod connecting plate 401 is provided with a positioning hole 403, and the upper end of the pipette connector 5 is provided with a positioning protrusion 504. The positioning protrusion 504 is sleeved with the positioning hole 403. The outer wall of the positioning protrusion 504 is provided with an annular groove 505. The side wall of the positioning hole 403 is provided with a threaded locking screw 404. The locking screw 404 is inserted into the annular groove 505 and presses against the positioning protrusion 504.

[0046] The positioning protrusion 504 has a notch 506 at the first air guide hole 503, and an air connector 507 is provided at the first air guide hole 503.

[0047] The air connector 507 is used to connect to an external negative pressure and positive pressure switching air pipe.

[0048] It also includes a third linear motion mechanism 3, which includes a base frame 201. The base frame 201 is provided with a second lead screw 303 and a third guide rail slider device 305. A second lead screw nut 304 is provided on one side of the lifting frame plate 301. The second lead screw nut 304 is sleeved with the second lead screw 303. One end of the second lead screw nut 304 is slidably connected to the base frame 201 through the third guide rail slider device 305. A third drive motor 302 is also provided on the base frame 201. The shaft end of the third drive motor 302 is connected to the second lead screw 303.

[0049] The system also includes a first linear moving mechanism 1 and a second linear moving mechanism 2. The first linear moving mechanism 1 includes a transverse frame 101 and a base plate 102. A first guide rail slider device 103 is provided on the base plate 102. The transverse frame 101 and the base plate 102 are slidably connected by the first guide rail slider device 103. A first lead screw 105 is provided on the base plate 102. A first drive motor 104 is provided at the shaft end of the first lead screw 105. A first lead screw nut 106 is also provided on the side of the transverse frame 101 near the base plate 102. 106 is sleeved with the first lead screw 105. The second linear movement mechanism 2 includes a cantilever 202. The cantilever 202 is provided with a second guide rail slider 203. The base frame 201 is slidably connected to the cantilever 202 through the second guide rail slider 203. The cantilever 202 is provided with a second drive motor 204 and a synchronous belt mechanism 205. The base frame 201 is provided with a synchronous belt clamping block 206. The second drive motor 204 drives the base frame 201 to move through the synchronous belt mechanism 205.

[0050] The first linear movement mechanism 1 is installed on the frame of the slide preparation machine. The first linear movement mechanism 1, the second linear movement mechanism 2 and the third linear movement mechanism 3 constitute a Cartesian movement mechanism, which drives the pipette connector 5 to move freely in three orthogonal directions, ensuring that liquid can be aspirated, pipetted and added at any position in the space of the slide preparation equipment.

[0051] The above embodiments are merely preferred technical solutions of this utility model and should not be considered as limitations on this utility model. The protection scope of this utility model should be the technical solution described in the claims, including equivalent substitutions of the technical features described in the claims. That is, equivalent substitutions and improvements within this scope are also within the protection scope of this utility model.

Claims

1. A quantitative liquid suction and discharge device, characterized in that: The utility model provides a pipette connector (5) comprising a connecting body (508) having a cavity (520) therein, a rotatable core (509) disposed in the cavity (520), a plurality of through quantitative cavities (510) disposed on the core (509), a plurality of slidable pistons (511) disposed in the quantitative cavities (510), the quantitative cavities (510) having different volumes, the quantitative cavities (510) being eccentrically arranged about the central axis of the core (509), a first air guide hole (503) and a second air guide hole (513) being disposed at the upper and lower ends of the cavity (520) and being eccentrically arranged about the central axis of the core (509), the central axes of the first air guide hole (503) and the second air guide hole (513) being aligned, a connecting nozzle (501) being further disposed at the lower end of the connecting body (508), the second air guide hole (513) being in communication with the connecting nozzle (501), and the connecting nozzle (501) being used for sleeving with a pipette (7).

2. The quantitative suction and discharge device according to claim 1, characterized by: The lower end of the connecting body (508) is open, the connecting body (508) is provided with a bottom cover (514) penetrating through the center, the bottom cover (514) is provided with a U-shaped inner lining cover (512), one end of the U-shaped inner lining cover (512) is open and faces the bottom cover (514), and the second air guide hole (513) is disposed on the U-shaped inner lining cover (512).

3. The quantitative suction and discharge device according to claim 1, characterized by: The outer wall of the core (509) is provided with a plurality of first magnetic tiles (515) in the circumferential direction, the magnetic poles of adjacent first magnetic tiles (515) are opposite, the outer wall of the connecting body (508) is sleeved with a rotatable sleeve (516), the inner wall of the sleeve (516) is provided with a plurality of second magnetic tiles (517) in the circumferential direction, and the magnetic poles of adjacent second magnetic tiles (517) are opposite.

4. The quantitative suction and discharge device according to claim 2, wherein: The bottom cover (514) is provided with the connecting nozzle (501), the connecting nozzle (501) is provided with a plurality of cylindrical sleeve joint parts (502) arranged in a stepped manner, and the cylindrical sleeve joint parts (502) are used for sleeving with the pipette (7).

5. The quantitative suction and discharge device according to claim 4, characterized in that: Further comprising a vertically movable lower extension rod connecting plate (401), the pipette connector (5) is arranged on the lower extension rod connecting plate (401), a flange edge (701) is arranged at the outer edge of the upper end of the pipette (7), one side of the pipette connector (5) is provided with a pipe dismounting rack (6), the lower end of the pipe dismounting rack (6) is provided with a push piece (601), and the end of the push piece (601) abuts against the outer wall of the connecting nozzle (501).

6. The quantitative suction and discharge device according to claim 5, wherein: Further comprising a third linear movement mechanism (3), the third linear movement mechanism (3) comprises a base frame (201), the base frame (201) is provided with a vertically movable lifting frame plate (301), the lifting frame plate (301) is provided with a lower extension rod (4), the lower end of the lower extension rod (4) is connected with the lower extension rod connecting plate (401), the base frame (201) is provided with a lower extension rod guide seat (402), the lower extension rod guide seat (402) is slidably sleeved with the lower extension rod (4), the upper end of the pipe dismounting rack (6) is provided with a lifting plate guide rod (602), the base frame (201) is further provided with a guide rod guide seat (603), the lifting plate guide rod (602) is slidably sleeved with the guide rod guide seat (603), and the upper end of the base frame (201) is provided with a spring balancer (8), the spring balancer (8) is hung with the upper end of the lifting plate guide rod (602).

7. The quantitative suction and discharge device according to claim 5, wherein: The lower end of the pipe rack (6) is provided with a hollow part (605), the hollow part (605) is provided with a hinge shaft (606), one end of the push piece (601) is hinged with the hinge shaft (606), the push piece (601) is provided with a first counterbore (607), the side of the hinge shaft (606) away from the pipette connector (5) is provided with a blocking piece (609), the blocking piece (609) is provided with a second counterbore (610), and a spring (608) is further arranged, one end of the spring (608) is inserted into the first counterbore (607) and abuts against the bottom end of the first counterbore (607), and the other end of the spring (608) is inserted into the second counterbore (610) and abuts against the bottom end of the second counterbore (610).

Images