L-tryptophan fermentation culture equipment
By introducing oxygen supply, mixing, and heating components into the L-tryptophan fermentation culture equipment, the problem of insufficient oxygen supply during fermentation was solved, the microbial growth rate and L-tryptophan yield were improved, and a more efficient fermentation process was achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-18
- Publication Date
- 2026-03-31
AI Technical Summary
Existing fermentation culture equipment lacks oxygen supply components during L-tryptophan fermentation, which slows down the growth rate of microorganisms and affects the yield of L-tryptophan.
An L-tryptophan fermentation culture device was designed, which includes an oxygen supply component, a mixing and scraping component, and a heating component. The oxygen supply component supplies oxygen to the fermenter after filtration by a fan and a filter plate. The mixing and scraping component ensures uniform mixing of raw materials and enhances flowability and mixing efficiency through stirring blades and scrapers. The heating component controls the temperature through heating wires.
It improved the growth rate of microorganisms, shortened the fermentation cycle, increased the yield of L-tryptophan, and improved mixing efficiency and temperature stability.
Smart Images
Figure CN224062780U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of L-tryptophan fermentation culture technology, and in particular to an L-tryptophan fermentation culture device. Background Technology
[0002] L-Tryptophan is an important essential amino acid that cannot be synthesized by humans and animals and must be obtained through diet. It has a variety of physiological functions in organisms and is a precursor for the synthesis of many bioactive substances. In the production of L-Tryptophan, the purpose of using fermentation culture equipment is to provide a fermentation environment for microorganisms, enabling them to grow and synthesize L-Tryptophan efficiently.
[0003] In existing fermentation and culture equipment, the material for L-tryptophan fermentation is first added into the fermentation and culture tank through the feeding port. Then, the fermentation tank is heated while the stirring component is started to mix the material for L-tryptophan fermentation, thereby completing the fermentation operation.
[0004] Although existing fermentation equipment can complete the fermentation and cultivation of L-tryptophan, the lack of oxygen supply components during the fermentation and cultivation of L-tryptophan slows down the growth rate of microorganisms, thus affecting the yield of L-tryptophan. Utility Model Content
[0005] To overcome the above deficiencies, this invention provides an L-tryptophan fermentation and cultivation device, which aims to improve the problem in the prior art where the lack of oxygen supply components during L-tryptophan fermentation and cultivation leads to a slowdown in the growth rate of microorganisms, thereby affecting the yield of L-tryptophan.
[0006] To achieve the above objectives, the present invention provides the following technical solution:
[0007] An L-tryptophan fermentation and culture device includes a support frame, a fermentation tank fixedly connected to the upper part of the support frame, a mixing and scraping component provided on the upper part of the fermentation tank, an oxygen supply component provided inside the support frame, and a heating component provided on the right side of the outside of the fermentation tank.
[0008] The oxygen supply assembly includes a filter box, which is fixedly connected inside the bracket. A mounting shell is fixedly connected to the outside of the filter box, and an air inlet hood is fixedly connected to the outside of the mounting shell. A fan is installed inside the mounting shell. A filter plate is inserted inside the filter box, and a delivery pipe is fixedly connected to the outside of the filter box. The top of the delivery pipe is fixedly connected to the inside of the fermenter.
[0009] Furthermore, the mixing and scraping assembly includes a motor, which is disposed at the upper part of the fermentation tank. A rotating rod is fixedly connected to the output end of the motor. The rotating rod is rotatably connected to the inside of the fermentation tank. Mounting blocks are fixedly connected to both outer sides of the rotating rod. Stirring blades are fixedly connected to the outer sides of both mounting blocks. Holes are opened on both inner sides of the multiple stirring blades. Mounting columns are fixedly connected to the outer sides of the upper mounting blocks and the rotating rod. Push rods are slidably connected inside the mounting columns. Springs are fixedly connected to the outer left sides of both push rods. Scrapers are fixedly connected to the outer right sides of both push rods. The two scrapers are in contact with the inner wall of the fermentation tank.
[0010] Furthermore, the heating assembly includes a controller, which is installed on the outside right side of the fermenter, and a heating wire is installed inside the fermenter, the heating wire being connected to the controller.
[0011] Furthermore, the filter box has an insertion hole on its exterior, and the filter plate is inserted into the insertion hole.
[0012] Furthermore, an mounting ring is fixedly connected to the upper part of the fermenter, and the motor is fixedly connected inside the mounting ring.
[0013] Furthermore, a feed inlet is fixedly connected to the upper right side of the fermentation tank, and a sealing cap is installed on the outside of the feed inlet.
[0014] Furthermore, a discharge port is fixedly connected to the lower part of the fermentation tank, and a control valve is installed outside the discharge port.
[0015] Furthermore, the support is made of stainless steel, and the fermentation tank is covered with an insulation shell.
[0016] This utility model has the following beneficial effects:
[0017] 1. In this utility model, when oxygen is supplied to the fermenter, the fan is started to draw in external air through the air inlet hood. After being filtered by the filter plate, the air is sent into the fermenter through the delivery pipe to complete the oxygen supply. This operation increases the growth rate of microorganisms, shortens the fermentation cycle, and increases the production of L-tryptophan.
[0018] 2. In this utility model, when mixing L-tryptophan raw materials, first open the sealing cover of the feed inlet, pour the raw materials into the fermentation tank, then close the sealing cover, start the motor, drive the rotating rod and the mounting block to rotate, and the stirring blades on the mounting block will rotate accordingly. The holes on the blades reduce liquid resistance and enhance the fluidity of the culture medium. At the same time, the mounting block drives the mounting column and the push rod to move. The scraper on the push rod is pressed tightly against the inner wall of the fermentation tank under the action of the spring, scraping off the attached raw materials and improving the mixing efficiency. Attached Figure Description
[0019] Figure 1 This is a three-dimensional view of an L-tryptophan fermentation culture device proposed in this utility model;
[0020] Figure 2 This is a schematic diagram of the discharge port structure of an L-tryptophan fermentation culture device proposed in this utility model;
[0021] Figure 3 This is a schematic diagram of the disassembled filter plate structure of an L-tryptophan fermentation culture device proposed in this utility model;
[0022] Figure 4 This is a schematic diagram of the internal structure of the fermenter in an L-tryptophan fermentation culture device proposed in this utility model.
[0023] Figure 5 This is a schematic diagram of the internal structure of the mounting shell of an L-tryptophan fermentation culture device proposed in this utility model.
[0024] Legend:
[0025] 1. Support frame; 2. Fermentation tank; 3. Feed inlet; 4. Discharge outlet; 5. Insulation shell; 6. Filter box; 7. Mounting shell; 8. Air inlet hood; 9. Fan; 10. Filter plate; 11. Insertion hole; 12. Conveying pipe; 13. Controller; 14. Heating wire; 15. Mounting ring; 16. Motor; 17. Mounting block; 18. Stirring blade; 19. Hole; 20. Mounting column; 21. Push rod; 22. Scraper; 23. Spring; 24. Rotating rod. Detailed Implementation
[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0027] Reference Figures 1-4This utility model provides an embodiment of an L-tryptophan fermentation and culture device, comprising a support frame 1, a fermentation tank 2 fixedly connected to the upper part of the support frame 1, a mixing and scraping component provided on the upper part of the fermentation tank 2, an oxygen supply component provided inside the support frame 1 for facilitating the injection of oxygen into the fermentation tank 2, a heating component provided on the right side of the outside of the fermentation tank 2 for facilitating the heating of the material inside the fermentation tank 2, a feed inlet 3 fixedly connected to the upper right side of the fermentation tank 2, a sealing cap installed on the outside of the feed inlet 3 for facilitating the pouring of raw materials, and a discharge outlet 4 fixedly connected to the lower part of the fermentation tank 2. The fermentation tank 2 is equipped with a control valve. The discharge port 4 facilitates the discharge of fermented material. The discharge rate of the discharge port 4 can be controlled by the control valve. The support 1 is made of stainless steel, which is highly corrosion-resistant and can prevent the equipment from rusting or corroding, thus improving the durability of the equipment. The fermentation tank 2 is covered with an insulation shell 5, which can reduce heat loss and make the internal temperature of the fermentation tank 2 more stable. The heating component includes a controller 13, which is installed on the right side of the outside of the fermentation tank 2. A heating wire 14 is installed inside the fermentation tank 2 and is connected to the controller 13. During heating, the controller 13 controls the heating wire 14 to generate heat, which is then transferred to the inside of the fermentation tank 2.
[0028] The oxygen supply assembly includes a filter box 6, which is fixedly connected inside the bracket 1. A mounting shell 7 is fixedly connected to the outside of the filter box 6, and an air inlet hood 8 is fixedly connected to the outside of the mounting shell 7. A fan 9 is installed inside the mounting shell 7. A filter plate 10 is inserted inside the filter box 6. The filter plate 10 facilitates the filtration of the intake air. A delivery pipe 12 is fixedly connected to the outside of the filter box 6. The top of the delivery pipe 12 is fixedly connected to the inside of the fermenter 2. The delivery pipe 12 facilitates the delivery of the filtered air to the inside of the fermenter 2. An insertion hole 11 is opened on the outside of the filter box 6. The filter plate 10 is inserted into the insertion hole 11 to facilitate the installation of the filter plate 10.
[0029] Specifically, when supplying oxygen to the fermenter 2, firstly, the blower 9 is started, generating suction to rapidly draw in outside air. The air then passes through the air inlet hood 8 and enters the filter box 6. The air inlet hood 8 initially intercepts larger particles of impurities. The drawn-in air is then transported to the filter box 6, where it is filtered through the filter plates 10 inserted inside. The filter plates 10 effectively remove fine particles, dust, microorganisms, and other pollutants from the air, ensuring that the air entering the fermenter 2 meets sterility standards. The filtered air is then transported to the interior of the fermenter 2 through the delivery pipe 12, providing sufficient oxygen for the microorganisms to ensure their normal growth and metabolism. This oxygen supply operation effectively increases the dissolved oxygen level of the fermentation broth, promotes the growth rate of microorganisms, accelerates the synthesis of L-tryptophan, thereby shortening the fermentation cycle and increasing the final yield of L-tryptophan.
[0030] Reference Figures 4-5 The mixing and scraping assembly includes a motor 16, which is located at the top of the fermenter 2. A rotating rod 24 is fixedly connected to the output end of the motor 16. The rotating rod 24 is rotatably connected inside the fermenter 2. Mounting blocks 17 are fixedly connected to both sides of the rotating rod 24, and stirring blades 18 are fixedly connected to the outside of each mounting block 17. The stirring blades 18 facilitate mixing of the materials inside the fermenter 2. Holes 19 are provided on both sides of the stirring blades 18 to reduce liquid resistance and enhance the fluidity of the culture medium during mixing. The upper mounting blocks 17 and the rotating rod 24 are also connected to the rotating rod 24. The moving rod 24 is fixedly connected to the external of the mounting column 20. The mounting column 20 is slidably connected to the inside of the mounting rod 21. The two push rods 21 are fixedly connected to the left side of the outside of the two push rods 21. The springs 23 are used to ensure that the scraper 22 is always in contact with the tank wall. The two push rods 21 are fixedly connected to the right side of the outside of the two push rods 21. The scraper 22 is used to scrape off the material attached to the tank wall. The two scrapers 22 are in contact with the inner wall of the fermentation tank 2. The upper part of the fermentation tank 2 is fixedly connected to the mounting ring 15. The motor 16 is fixedly connected inside the mounting ring 15. The mounting ring 15 is used to fix the motor 16.
[0031] Specifically, when mixing the L-tryptophan raw material, firstly, unscrew the sealing cap installed on the outside of the feed inlet 3. Then, pour the L-tryptophan raw material into the fermenter 2 through the feed inlet 3. Next, replace the sealing cap on the outside of the feed inlet 3. Then, start the motor 16. The motor 16 drives the rotating rod 24 fixed at the output end to rotate. The rotation of the rotating rod 24 drives the externally fixed mounting blocks 17 to rotate. The rotation of the two mounting blocks 17 drives the externally fixed stirring blades 18 to rotate. The stirring blades 18 have holes 19 on both sides inside. The holes 19 can reduce liquid resistance and make the culture medium more fluid during the stirring process. At the same time, when the mounting blocks 17 rotate, they drive the stirring blades 18 to rotate. The externally fixed mounting column 20 moves synchronously, which in turn moves the internally sliding push rod 21 synchronously. The push rod 21 in turn moves the externally fixed scraper 22 on the right side synchronously. Under the force of the spring 23, the scraper 22 is always in contact with the inner wall of the fermenter 2, which can effectively scrape off the L-tryptophan raw material attached to the tank wall, thereby avoiding waste of raw material. Through this design, the scraper 22 not only ensures that the raw material is fully mixed, but also further improves the uniformity and efficiency of stirring, ensuring that each part of the L-tryptophan raw material can fully contact the microorganisms, thereby promoting the smooth progress of the fermentation process and improving the mixing efficiency.
[0032] Working principle: When supplying oxygen to the fermenter 2, firstly, the blower 9 is started, generating suction. Then, external air is drawn in through the air inlet hood 8 and delivered to the inside of the filter box 6. Next, the filter plates 10 inserted inside the filter box 6 filter impurities in the air. Then, the filtered air is delivered to the inside of the fermenter 2 through the delivery pipe 12, thus completing the oxygen supply operation. This achieves the goal of drawing in and filtering external air through the blower 9 and finally delivering it to the inside of the fermenter 2 during the fermentation of L-tryptophan, thereby increasing the growth rate of microorganisms, shortening the fermentation cycle, and thus increasing the yield of L-tryptophan.
[0033] When mixing the L-tryptophan raw material, firstly, unscrew the sealing cap installed on the outside of the feed inlet 3. Then, pour the L-tryptophan raw material into the fermenter 2 through the feed inlet 3. Next, replace the sealing cap on the outside of the feed inlet 3. Then, start the motor 16. The motor 16 drives the rotating rod 24 fixed at the output end to rotate. The rotation of the rotating rod 24 drives the external fixed mounting blocks 17 to rotate. The rotation of the two mounting blocks 17 drives the externally fixed stirring blades 18 to rotate. The stirring blades 18 have holes 19 on both sides inside to reduce liquid resistance and allow the culture medium to flow during stirring. The mixing process is more efficient. Simultaneously, as the mounting block 17 rotates, it moves the externally fixed mounting column 20 in sync. The movement of the mounting column 20 moves the internally sliding push rod 21 in sync. The movement of the push rod 21 moves the externally fixed scraper 22 on the right side in sync. Under the force of the spring 23, the scraper 22 remains in contact with the inner wall of the fermenter 2. This allows for reduced liquid resistance during L-tryptophan mixing by opening holes 19 on both sides of the stirring blades 18, resulting in greater fluidity of the culture medium during mixing. It also facilitates the scraping of materials adhering to the tank wall, thereby improving mixing efficiency.
[0034] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A L-tryptophan fermentation culture apparatus comprising a support (1), characterized in that: The upper part of the support (1) is fixedly connected to the fermentation tank (2), the upper part of the fermentation tank (2) is provided with a mixing scraping component, the inside of the support (1) is provided with an oxygen supply component, and the right side of the outside of the fermentation tank (2) is provided with a heating component; The oxygen supply assembly includes a filter box (6), which is fixedly connected inside the bracket (1). A mounting shell (7) is fixedly connected to the outside of the filter box (6). An air inlet hood (8) is fixedly connected to the outside of the mounting shell (7). A fan (9) is installed inside the mounting shell (7). A filter plate (10) is inserted inside the filter box (6). A conveying pipe (12) is fixedly connected to the outside of the filter box (6). The top of the conveying pipe (12) is fixedly connected to the inside of the fermenter (2).
2. The L-tryptophan fermentation culture apparatus according to claim 1, characterized by: The mixing and scraping assembly includes a motor (16), which is located at the top of the fermentation tank (2). A rotating rod (24) is fixedly connected to the output end of the motor (16). The rotating rod (24) is rotatably connected inside the fermentation tank (2). Mounting blocks (17) are fixedly connected to both sides of the rotating rod (24). Stirring blades (18) are fixedly connected to both sides of the two mounting blocks (17). Holes (19) are opened on both sides of the interior of the multiple stirring blades (18). Mounting columns (20) are fixedly connected to both the upper mounting block (17) and the rotating rod (24). Push rods (21) are slidably connected inside the mounting columns (20). Springs (23) are fixedly connected to the left side of both push rods (21). Scrapers (22) are fixedly connected to the right side of both push rods (21). The two scrapers (22) are in contact with the inner wall of the fermentation tank (2).
3. The L-tryptophan fermentation culture apparatus according to claim 1, characterized by: The heating assembly includes a controller (13), which is installed on the outside right side of the fermentation tank (2). A heating wire (14) is installed inside the fermentation tank (2) and is connected to the controller (13).
4. The L-tryptophan fermentation culture apparatus according to claim 1, characterized by: The filter box (6) has an insertion hole (11) on the outside, and the filter plate (10) is inserted into the insertion hole (11).
5. The L-tryptophan fermentation culture apparatus according to claim 2, characterized by: The fermenter (2) is fixedly connected to an installation ring (15) on its upper part, and the motor (16) is fixedly connected inside the installation ring (15).
6. The L-tryptophan fermentation culture apparatus according to claim 1, characterized by: The fermenter (2) is fixedly connected to the upper right side of the feed inlet (3), and a sealing cover is installed on the outside of the feed inlet (3).
7. The L-tryptophan fermentation culture equipment according to claim 1, characterized in that: The fermenter (2) is fixedly connected to a discharge port (4) at the bottom, and a control valve is installed on the outside of the discharge port (4).
8. The L-tryptophan fermentation culture equipment according to claim 1, characterized in that: The support (1) is made of stainless steel, and the fermentation tank (2) is covered with an insulation shell (5).