Novel structure test paper for electrochemical biosensor

Abstract

The utility model relates to test paper, and discloses novel structure test paper for an electrochemical biosensor, which comprises an HCT detection area (1) and a substrate detection area (2), the HCT detection area comprises an HCT detection electrode (11) and a first counter electrode (12), and the HCT detection electrode and the first counter electrode form an electrode loop; the substrate detection area (2) comprises a substrate detection working electrode (21) and a second counter electrode (22), and the substrate detection working electrode (21) and the second counter electrode (22) form an electrode loop; and the first counter electrode and the second counter electrode are connected and share one counter electrode (3). According to the test paper, a single counter electrode is divided into two parts, and establishment and partitioning of double electrochemical test loops are realized under the condition that a counter electrode passage is not additionally increased; the design is high in universality and is used for blood glucose detection, and substances such as uric acid, cholesterol and blood ketone can be designed by using the model; and high-stability detection of HCT and detection sample concentration is realized.

Description

TECHNICAL FIELD

[0001] The utility model relates to, especially for a new structure test paper for electrochemical biosensor. BACKGROUND

[0002] On the market, disposable electrochemical biosensors (blood glucose test paper, uric acid test paper, blood ketone test paper and total cholesterol test paper, etc.) have been used for the determination of the concentration of substrates such as blood glucose, uric acid, blood ketone and total cholesterol in human blood. The basic principle is that when the blood sample enters the test paper reaction zone, under the action of a certain electric potential, the reaction current generated by the corresponding substrate is converted into the monitoring of the corresponding substrate concentration.

[0003] The accuracy of such electrochemical test paper on the market is significantly affected by the hematocrit (HCT) of human blood. In some environments, the influence even far exceeds that of other factors. Special groups such as infants have higher hematocrit, and the error is greater. Therefore, eliminating the influence of hematocrit is one of the keys to improving the accuracy of such products. The electrochemical test paper with HCT correction on the market generally uses impedance to determine the impedance of blood, so most of them use an HCT working electrode and an independent HCT counter electrode to form an electrode circuit, or an HCT working electrode and a substrate detection working electrode share a counter electrode to form an electrode circuit to realize detection. The former will lead to more complex design and higher instrument cost, and because of the additional electrode lead, the entire test paper needs a relatively wider width, further increasing the cost; the latter shares a counter electrode, which needs to cover active substances such as enzymes or electron mediators with the working electrode to form a loop, catalyzing and promoting the detection of the oxidation-reduction current of the substrate. However, due to the presence of active substances, when an alternating voltage is applied, an additional direct current signal will be generated, resulting in an abnormally low impedance value, thus reducing the stability of the HCT electrode and causing the HCT measurement result to deviate, ultimately causing the corrected concentration to deviate.

[0004] For example, the application number CN202220702047.2 is a detection test paper with high precision without code adjustment, which is most similar to the present scheme.

[0005] In this patent, conductivity is used to determine the HCT value of blood. The HCT detection area is a detection loop composed of two electrodes, that is, there is a separate HCT working electrode and an HCT counter electrode to form a loop for blood impedance testing. The other working electrode and the other counter electrode form a second loop, which is responsible for measuring the current value of the small molecule substrate in the blood. By constructing a linear relationship between the measured conductivity and the corresponding HCT value, the compensation of the measured current value under different HCT is completed, and the final test result is corrected. Utility model content

[0006] The utility model discloses to the prior art's shortcoming provides a novel structure test paper for electrochemical biosensor.

[0007] In order to solve above-mentioned technical problem, the utility model solves through following technical scheme:

[0008] A novel structure test paper for electrochemical biosensor, including

[0009] HCT detection area, HCT detection area includes HCT detection electrode and first pair of electrode, and HCT detection electrode and first pair of electrode constitute electrode loop, and the alternating voltage with amplitude 0.05-0.5V is applied in electrode loop;

[0010] Substrate detection area, substrate detection area includes substrate detection working electrode and second pair of electrode, and substrate detection working electrode and second pair of electrode constitute electrode loop, and the direct current voltage with amplitude 0.1-0.5V is applied in electrode loop;

[0011] First pair of electrode and second pair of electrode are connected and share a pair of electrode.

[0012] As preferred, HCT detection area is the test paper layer without enzyme or electron medium.

[0013] As preferred, substrate detection area is the test paper layer containing enzyme or electron medium.

[0014] As preferred, including full of electrode, full of electrode is located in the inside of HCT detection area and is arranged in the side of substrate detection area.

[0015] The utility model discloses since adopting above technical scheme, has remarkable technical effect:

[0016] (1) this test paper will single pair of electrode divide into two, realizes the establishment and partition of double electrochemical test loop under the condition of not additional increase pair of electrode passage;

[0017] (2) the design versatility is strong, not only is used in blood sugar detection, uric acid, cholesterol, blood ketone etc., and substance can use this model to design;Realize the high stability detection of HCT and detection sample concentration. DETAILED DESCRIPTION

[0018] Fig. 1 It is the structure schematic diagram of the utility model.

[0019] Fig. 2 It is the structure schematic diagram of the utility model.

[0020] Fig. 3 It is the structure schematic diagram of prior art test paper.

[0021] The part names that the following numbers in the above drawing refer to are as follows:

[0022] 1 - HCT detection zone, 11 - HCT detection electrode, 12 - first counter electrode

[0023] 2 - substrate detection zone, 21 - substrate detection working electrode, 22 - second counter electrode

[0024] 3 - counter electrode

[0025] 4 - full electrode DETAILED DESCRIPTION

[0026] The utility model will be described in further detail below with reference to the accompanying drawings Figs. 1-3 The utility model will be described in further detail below with reference to the accompanying drawings

[0027] Example 1

[0028] A novel structure test paper for electrochemical biosensor comprises

[0029] HCT detection zone 1, HCT detection zone 1 includes HCT detection electrode 11 and first counter electrode 12, and HCT detection electrode 11 and first counter electrode 12 form an electrode loop;

[0030] Substrate detection zone 2, substrate detection zone 2 includes substrate detection working electrode 21 and second counter electrode 22, and substrate detection working electrode 21 and second counter electrode 22 form an electrode loop;

[0031] First counter electrode 12 and second counter electrode 22 are connected and share a counter electrode 3, and first counter electrode 12 and second counter electrode 22 are formed by splitting the same counter electrode 3, so that the cost can be reduced without additionally increasing an independent counter electrode, and meanwhile, second counter electrode 22 does not need to be covered with active substances such as enzymes or electronic mediators, and thus cannot directly generate a large redox current signal, so that the influence of the reagent layer on the counter electrode on the HCT detection signal can be avoided, and the detection stability can be improved.

[0032] HCT detection zone 1 is a test paper layer without enzymes or electronic mediators, and in the embodiment, HCT detection zone 1 is a test paper layer without enzymes, and in other embodiments, HCT detection zone 1 can be a test paper layer with electronic mediators.

[0033] Substrate detection zone 2 is a test paper layer with enzymes or electronic mediators, and in the embodiment, substrate detection zone 2 is a test paper layer with enzymes, and in other embodiments, substrate detection zone 2 is a test paper layer with electronic mediators.

[0034] Full electrode 4 is included, and full electrode 4 is located on the inner side of HCT detection zone and is arranged on the side edge of substrate detection zone 2.

[0035] The following table is the detection result of HCT at different temperatures, Fig. 2 The control group is, Fig. 3For the experimental group, the control group and the experimental group structure of the blood glucose test paper, respectively, in 20%, 40% and 60% HCT blood samples under test impedance value.

[0036] From the results, the repeatability of the control group test results under the conditions of 5℃, 25℃ and 45℃ is obviously better than that of the experimental group.

[0037] At the same time, it can be seen that the impedance gradient difference corresponding to 20%-60% of the control group is greater than that of the experimental group, indicating that the control group structure can also increase the impedance gradient to a certain extent, further increasing the HCT test sensitivity.

[0038]

[0039]

Claims

1. A novel structured test strip for electrochemical biosensors, characterized in that: Comprising a HCT detection zone (1) comprising a HCT detection electrode (11) and a first counter electrode (12), the HCT detection electrode (11) and the first counter electrode (12) forming an electrode loop; a substrate detection zone (2) comprising a substrate detection working electrode (21) and a second counter electrode (22), the substrate detection working electrode (21) and the second counter electrode (22) forming an electrode loop; the first counter electrode (12) and the second counter electrode (22) are connected and share one counter electrode (3).

2. A novel structured test strip for electrochemical biosensor according to claim 1, wherein: the HCT detection zone (1) is an enzyme-free or mediator-free test paper layer.

3. A novel structured test strip for electrochemical biosensor as claimed in claim 1, wherein: the substrate detection zone (2) is an enzyme-containing or mediator-containing test paper layer.

4. A novel structured test strip for electrochemical biosensor according to claim 1, wherein: comprising a filling electrode (4) located inside the HCT detection zone and arranged at the side of the substrate detection zone (2).

Images