Automatic isothermal amplification system

Abstract

The utility model discloses an automatic constant-temperature amplification system, and relates to the technical field of amplification systems, the automatic constant-temperature amplification system further comprises a base, the top of the base is rotatably connected with a rotating rod, the top end of the rotating rod is fixedly connected with a constant-temperature box, the outer surface of the constant-temperature box is provided with a plurality of clamping grooves, separator bodies are arranged in the clamping grooves, and the top of each separator body is provided with a plurality of holes for placing test tubes; a positioning mechanism used for preventing the separator body from falling off is arranged at the top of the constant-temperature box and comprises a fixing rod, the fixing rod is fixedly connected to the top of the constant-temperature box, the outer side of the fixing rod is slidably sleeved with a sliding ring, the two sides of the sliding ring are both fixedly connected with connecting plates, and the outer side of the constant-temperature box is slidably sleeved with a baffle ring used for blocking the separator body; the side, away from the sliding ring, of the connecting plate is fixedly connected with the inner wall of the baffle ring, by arranging the positioning mechanism, the separator body can be effectively fixed when the constant-temperature box is rotated, the phenomenon that the separator body is thrown out of the clamping groove is avoided, and the safety of the system in the operation process is improved.

Description

Technical Field

[0001] This application relates to the field of amplification system technology, and more specifically, to an automated isothermal amplification system. Background Technology

[0002] Compared to traditional PCR, isothermal amplification primarily utilizes the activity of recombinant enzymes, allowing amplification at lower temperatures without nucleic acid denaturation or annealing. Therefore, it eliminates the need for expensive equipment and avoids enzyme degradation caused by high temperatures. Furthermore, isothermal amplification eliminates the need for excessive primers, reducing the likelihood of primer-template mismatches or primer dimer formation. Isothermal amplification technology is gaining increasing attention.

[0003] The prior art publication CN222182278U provides a isothermal amplification nucleic acid reagent separator. It features four separator bodies. By using handles, the corresponding separator body is pulled out of its slot on the separation chamber. Different test tubes are then placed into the test tube holes on different separator bodies, achieving separation of the isothermal amplification nucleic acid reagent test tubes. This is simple and convenient. After placing the test tubes, the separator body is then inserted back into its slot on the separation chamber. The separation chamber is rotated by a turntable and a rotating rod. The turntable rotates within the second movable hole, and the rotating rod rotates within the first movable hole, facilitating the retrieval of test tubes from different separator bodies from the same location, effectively improving the ease of use of this invention.

[0004] Although the device has many beneficial effects, it still has the following problems: when the operator rotates the separation box, it is difficult to fix the separator body, which makes it very easy for the separator body to be thrown out of the slot, thus affecting the operator's work progress in separating nucleic acid reagents. In view of this, we propose an automated isothermal amplification system. Utility Model Content

[0005] This application provides an automated isothermal amplification system that solves the technical problem in the prior art where it is difficult to fix the separator body when the operator rotates the separation chamber, making it easy for the separator body to be thrown out of the slot, which can affect the operator's progress in separating nucleic acid reagents. The system effectively fixes the separator body when the isothermal chamber is rotated, preventing the separator body from being thrown out of the slot, thus improving the safety of the system during operation, significantly increasing work efficiency, and reducing the risk of experimental interruption caused by the displacement or falling of the separator body.

[0006] This application provides an automated isothermal amplification system, including: a base;

[0007] The base is rotatably connected to the top of the base, and a constant temperature chamber is fixedly connected to the top of the rotating rod. The outer surface of the constant temperature chamber is provided with multiple slots, and a separator body is provided in the slots. The top of the separator body is provided with multiple openings for placing test tubes.

[0008] The top of the constant temperature chamber is equipped with a positioning mechanism to prevent the separator body from falling off;

[0009] The positioning mechanism includes a fixed rod, which is fixedly connected to the top of the constant temperature chamber. A slip ring is slidably sleeved on the outside of the fixed rod. Connecting plates are fixedly connected to both sides of the slip ring. A retaining ring for blocking the separator body is slidably sleeved on the outside of the constant temperature chamber. The side of the connecting plate away from the slip ring is fixedly connected to the inner wall of the retaining ring.

[0010] By adopting the above technical solution and setting a positioning mechanism, the separator body can be effectively fixed when the constant temperature chamber is rotated, avoiding the phenomenon that the separator body is thrown out of the slot, improving the safety of the system operation, significantly improving work efficiency, and reducing the risk of experimental interruption caused by the displacement or falling of the separator body.

[0011] Optionally, a limiting plate is fixedly connected to the top of the fixing rod, and the size of the limiting plate is larger than the size of the inner side of the slip ring.

[0012] By adopting the above technical solution, in actual operation, when the operator pulls the connecting plate to drive the slip ring to rise, the limiting plate plays a restrictive role, preventing the slip ring from falling off the outside of the fixed rod due to excessive movement, thereby ensuring the normal working process of the positioning mechanism and achieving the effect of improving the stability and reliability of the positioning mechanism.

[0013] Optionally, a spring is fixedly connected to the top of the slip ring, and the top of the spring is fixedly connected to the bottom of the limiting plate.

[0014] By adopting the above technical solution, and by incorporating a spring, the spring is compressed when the slip ring rises. After releasing the first handle, the compressed spring automatically rebounds, pushing the slip ring and retaining ring down to the designated position, ensuring that the retaining ring fits tightly against the outside of the separator body, thus simplifying the operation process. Simultaneously, the elastic restoring force of the spring ensures significant resistance to the upward movement of the retaining ring, thereby achieving automatic reset and enhancing the fixing effect.

[0015] Optionally, the base has a groove on its top, and a ball bearing is installed in the groove. The rotating rod is rotatably connected to the groove through the ball bearing.

[0016] By adopting the above technical solution, the ball bearing significantly reduces the frictional resistance between the rotating rod and the base when the operator rotates the constant temperature chamber, making the rotation smoother and less strenuous. Furthermore, the ball bearing also has a certain load-bearing capacity, which further enhances the overall stability of the device, achieving the effects of optimizing the operating experience and extending the equipment's service life.

[0017] Optionally, multiple mounting plates are fixedly connected to the outer surface of the base, and suction cups are fixedly connected to the bottom of the mounting plates.

[0018] By employing the above technical solution, the suction cup can firmly adhere the base to a stable work surface, preventing displacement or tipping due to external vibration or rotational torque. In practical use, especially when frequent adjustments to the temperature control chamber angle are required or high-speed rotation operations are performed, this fixing method is particularly important, thereby improving the overall stability and safety of the base.

[0019] One or more technical solutions provided in the embodiments of this application have at least the following technical effects or advantages:

[0020] 1. By setting up a positioning mechanism, the separator body can be effectively fixed when the constant temperature chamber is rotated, avoiding the phenomenon that the separator body is thrown out of the slot, which improves the safety of the system during operation, significantly improves work efficiency, and reduces the risk of experimental interruption caused by the displacement or falling of the separator body.

[0021] 2. By incorporating a spring, the spring is compressed as the slip ring rises. Upon releasing the first handle, the compressed spring automatically rebounds, pushing the slip ring and retaining ring down to the designated position. This ensures the retaining ring fits tightly against the outside of the separator body, simplifying the operation. Simultaneously, the spring's restoring force ensures significant resistance to the retaining ring's upward movement, achieving automatic reset and enhancing the fixing effect.

[0022] 3. By incorporating a mounting plate and suction cups, the suction cups securely adhere the base to a stable work surface, preventing displacement or tipping due to external vibrations or rotational torque. In practical use, this fixing method is particularly important when frequent adjustments to the temperature control chamber angle or high-speed rotation operations are required, thus enhancing the overall stability and safety of the base. Attached Figure Description

[0023] Figure 1 This is a first-view schematic diagram of the overall structure of an automated isothermal amplification system according to an embodiment of this application;

[0024] Figure 2 This is a second-view schematic diagram of the overall structure of an automated isothermal amplification system according to an embodiment of this application;

[0025] Figure 3 This is a schematic diagram of the separator body of an automated isothermal amplification system according to an embodiment of this application;

[0026] The following are the labels in the diagram: 1. Base; 2. Rotating rod; 3. Positioning mechanism; 31. Fixing rod; 32. Slip ring; 33. Connecting plate; 34. Retaining ring; 35. Limiting plate; 36. Spring; 4. Constant temperature chamber; 5. Separator body; 6. Mounting plate; 7. Suction cup. Detailed Implementation

[0027] The present application will be further described in detail below with reference to the accompanying drawings.

[0028] Reference Figure 1 , Figure 2 and Figure 3 This application discloses an automated isothermal amplification system. The automated isothermal amplification system includes: a base 1;

[0029] A rotating rod 2 is rotatably connected to the top of the base 1, and a constant temperature chamber 4 is fixedly connected to the top of the rotating rod 2. Multiple slots are opened on the outer surface of the constant temperature chamber 4, and a separator body 5 is provided in the slots. Multiple openings for placing test tubes are opened on the top of the separator body 5.

[0030] The top of the constant temperature chamber 4 is equipped with a positioning mechanism 3 to prevent the separator body 5 from falling off;

[0031] The positioning mechanism 3 includes a fixing rod 31, which is fixedly connected to the top of the constant temperature chamber 4. A slip ring 32 is slidably sleeved on the outside of the fixing rod 31. Connecting plates 33 are fixedly connected to both sides of the slip ring 32. A retaining ring 34 is slidably sleeved on the outside of the constant temperature chamber 4 to block the separator body 5. The side of the connecting plate 33 away from the slip ring 32 is fixedly connected to the inner wall of the retaining ring 34, so that the separator body 5 can be prevented from falling out of the slot during the rotation of the constant temperature chamber 4.

[0032] Reference Figure 1 and Figure 2 A limiting plate 35 is fixedly connected to the top of the fixing rod 31. The size of the limiting plate 35 is larger than the size of the inner side of the slip ring 32, so as to prevent the slip ring 32 from falling off the outside of the fixing rod 31.

[0033] Reference Figure 1 and Figure 2 A spring 36 is fixedly connected to the top of the slip ring 32, and the top of the spring 36 is fixedly connected to the bottom of the limiting plate 35, so as to prevent the slip ring 32 from moving at will.

[0034] Reference Figure 1 and Figure 2 The top of the connecting plate 33 is fixedly connected to a first handle, which makes it easy for the staff to pull the connecting plate 33 and the retaining ring 34 to move upward.

[0035] Reference Figure 1 and Figure 3 A second handle is fixedly connected to the outer wall of the separator body 5, which makes it easy for staff to remove the separator body 5 from the slot.

[0036] Reference Figure 1 and Figure 2 The outer wall of the separator body 5 is in contact with the inner wall of the retaining ring 34, so that the separator body 5 is blocked by the retaining ring 34 and will not shake freely in the slot.

[0037] Reference Figure 1 and Figure 2 In the initial state, the lowest point of the bottom of the retaining ring 34 is lower than the highest point of the top of the separator body 5, so that the retaining ring 34 can block the separator body 5.

[0038] Reference Figure 1 and Figure 2 The base 1 has a groove on its top, and a ball bearing is installed in the groove. The rotating rod 2 is rotatably connected to the groove through the ball bearing, which helps to reduce the friction when the rotating rod 2 rotates.

[0039] Reference Figure 1 and Figure 2 Multiple mounting plates 6 are fixedly connected to the outer surface of the base 1, and suction cups 7 are fixedly connected to the bottom of the mounting plates 6, so as to make it easy to attach the base 1 to the counter and ensure the stability of the base 1.

[0040] The implementation principle of an automated isothermal amplification system in this application embodiment is as follows: when nucleic acid reagent separation is required, the base 1 is first attached to a stable work surface by suction cup 7 to ensure the stability of the entire device.

[0041] Subsequently, the staff can first pull the first handle to move the connecting plate 33 upward. The movement of the connecting plate 33 can move the retaining ring 34 and the slip ring 32, so that the slip ring 32 squeezes the spring 36, thereby moving the retaining ring 34 to a specified height so that the retaining ring 34 will no longer block the slot. Then, the staff places the test tube into the opening of the separator body 5. After the test tube is placed, the separator body 5 is inserted into the slot on the outer surface of the constant temperature box 4.

[0042] Then, the operator can release the first handle, causing the compressed spring 36 to rebound, which in turn moves the slip ring 32 and connecting plate 33 downwards. The movement of the connecting plate 33 moves the retaining ring 34, positioning it to block the slot and the separator body 5. This ensures the inner wall of the retaining ring 34 fits tightly against the outer side of the separator body 5, effectively preventing it from detaching due to the rotation of the temperature chamber 4. After completing these steps, the operator can safely rotate the temperature chamber 4 to conduct the experiment while keeping the separator body 5 stable.

[0043] This application provides an automated isothermal amplification system. By setting a positioning mechanism 3, the separator body 5 can be effectively fixed when the isothermal chamber 4 is rotated, avoiding the phenomenon that the separator body 5 is thrown out of the slot. This improves the safety of the system during operation, significantly increases work efficiency, and reduces the risk of experimental interruption caused by the displacement or falling of the separator body 5.

Claims

1. An automated isothermal amplification system comprising a base (1), characterised in that: The base (1) top rotationally connected with the rotating rod (2), the rotating rod (2) top fixedly connected with the thermostat (4), the thermostat (4) outer surface is provided with a plurality of clamping grooves, the clamping groove is equipped with the separator body (5), the separator body (5) top is provided with a plurality of openings for placing test tube; The thermostat (4) top is equipped with the positioning mechanism (3) for preventing the separator body (5) from falling off; The positioning mechanism (3) includes a fixed rod (31), the fixed rod (31) is fixedly connected to the top of the thermostat (4), the fixed rod (31) outside is slidably sleeved with a sliding ring (32), the sliding ring (32) both sides are fixedly connected with a connecting plate (33), the thermostat (4) outside is slidably sleeved with a blocking ring (34) for blocking the separator body (5), the connecting plate (33) away from the sliding ring (32) one side and the inner wall of the blocking ring (34) are fixedly connected. The fixed rod (31) top fixedly connected with a limiting plate (35), the limiting plate (35) size is greater than the size of the sliding ring (32) inside.

2. The automated, isothermal amplification system of claim 1, wherein, The sliding ring (32) top fixedly connected with a spring (36), the spring (36) top and the limiting plate (35) bottom fixedly connected.

3. The automated, isothermal amplification system of claim 2, wherein, The base (1) top recess is provided, the recess is installed with a ball bearing, the rotating rod (2) is rotationally connected in the recess through the ball bearing.

4. The automated, isothermal amplification system of claim 1, wherein: The base (1) outer surface is fixedly connected with a plurality of mounting plates (6), the mounting plate (6) bottom is fixedly connected with a suction cup (7).

5. The automated, isothermal amplification system of claim 1, wherein: ​

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