Device for liquid strain propagation

Abstract

The utility model relates to the technical field of liquid strain propagation, in particular to liquid strain propagation equipment which comprises a tank body, the tank body is connected with a tank cover through a fixing mechanism, a stirring mechanism and a filtering mechanism are fixed on the tank cover, a protruding block is installed on the stirring mechanism, the filtering mechanism comprises a sealing cover and a fixing ring, and the sealing cover is connected with the fixing ring. A fixing ring is fixedly installed on the tank cover, a clamping groove is formed in the fixing ring, a clamping ring is installed at the clamping groove in a clamped mode, a pull rod and a filter cylinder are fixedly connected to the clamping ring, a sealing cover is installed on the side wall of the fixing ring in a threaded mode, and a protruding block makes contact with the side wall of the filter cylinder; the clamping ring is clamped in the clamping groove formed in the fixing ring, the filter cylinder can be communicated with the interior of the tank body, insoluble substances in culture medium raw materials can be filtered out when a culture medium is added into the tank body, the protruding block rotates to make contact with the filter cylinder, and a filter screen of the filter cylinder can be prevented from being blocked.

Description

Technical Field

[0001] This utility model relates to a propagation device, specifically a device for propagating liquid microorganisms, and belongs to the field of liquid microorganism propagation technology. Background Technology

[0002] Liquid culture fermenters (also known as liquid culture tanks or bioreactors) are specifically designed for the propagation of liquid microbial cultures and are core equipment for industrial production or large-scale cultivation of microorganisms (such as bacteria, yeast, and fungal mycelia). A liquid culture fermenter introduces a small amount of original inoculum (such as slant culture or shake flask seed culture) into the tank, and through optimized culture conditions (temperature, pH, dissolved oxygen, agitation, etc.), achieves massive proliferation of cells or mycelia.

[0003] However, in the process of liquid culture propagation, adding liquid culture medium is a key step to ensure efficient proliferation and maintain metabolic activity of the strain. Since liquid culture medium may contain insoluble substances, when these substances are added to the liquid culture, they will collide with the bacteria during stirring or aeration, which can easily lead to mycelial breakage and abnormal branching. Utility Model Content

[0004] The purpose of this invention is to provide a liquid microbial culture propagation device to solve the above problems. By placing the retaining ring into the groove provided in the fixing ring, the filter cartridge can be connected to the inside of the tank. This allows for the filtration of insoluble substances in the culture medium raw materials when culture medium is added to the tank. Furthermore, the rotating protrusion contacts the filter cartridge, preventing the filter screen of the filter cartridge from becoming clogged.

[0005] This utility model achieves the above-mentioned objective through the following technical solution: a liquid bacterial culture propagation device includes a tank body, which is connected to a tank cover via a fixing mechanism. A stirring mechanism and a filtering mechanism are fixed on the tank cover. A protrusion is installed on the stirring mechanism. The filtering mechanism includes a sealing cap and a fixing ring. A fixing ring is fixedly installed on the tank cover. A slot is provided in the fixing ring. A retaining ring is engaged in the slot. A pull rod and a filter cylinder are fixedly connected to the retaining ring. A sealing cap is threaded onto the side wall of the fixing ring. The protrusion contacts the side wall of the filter cylinder.

[0006] Preferably, the retaining ring and the fixing ring are engaged, the sealing cap is in contact with the top of the can lid, and the height of the retaining ring is less than the height of the retaining groove.

[0007] Preferably, the filter cartridge is connected to the interior of the tank through the tank cover, and the bottom end of the filter cartridge is arranged in an arc shape, and the part of the protrusion that contacts the filter cartridge is also arranged in an arc shape.

[0008] Preferably, the stirring mechanism includes a motor and a rotating shaft. The motor is fixedly installed on the top of the tank lid, and the output end of the motor is fixedly connected to the rotating shaft through a coupling. Several rotating rods and stirring rods are fixedly installed on the side wall of the rotating shaft.

[0009] Preferably, a plurality of the rotating rods and stirring rods are arranged in a ring at equal intervals, and the protrusions are fixedly connected to the rotating rods.

[0010] Preferably, the rotating shaft is rotatably connected to the can lid, and the rotating rod is arranged in an arc shape.

[0011] Preferably, the fixing mechanism includes an mounting block and a rotating block. Several mounting blocks are fixedly connected in a ring at equal intervals on the side wall of the tank. A rotating block is rotatably connected inside the mounting block. A locking rod is fixedly connected to the rotating block. A stop block is threadedly connected to the locking rod. A locking block is fixedly connected to the tank cover. The locking rod and the locking block are engaged.

[0012] Preferably, the locking rod is located at the top of the rotating block, the top of the locking rod is spherical, and the abutment is hexagonal.

[0013] Preferably, the part of the card block that contacts the card rod has an arc-shaped structure, and the bottom end of the abutment block abuts against the card block.

[0014] Preferably, a plurality of supports are fixedly connected in a ring at equal intervals on the tank body, and the supports are located on the side wall at the bottom of the tank body.

[0015] The beneficial effects of this utility model are as follows: After the can lid is snapped onto the top of the can, the pull rod fixed to the inner wall of the snap ring can be held, and the snap ring can be snapped into the slot provided in the fixing ring. Since the fixing ring is installed at the top of the can lid, the snap ring can be limited by the fixing ring. A filter cartridge is fixedly installed at the bottom of the snap ring. The filter cartridge is connected to the inside of the can through the can lid. When liquid culture medium needs to be added to the can, the sealing cover installed on the side wall of the fixing ring can be rotated open. When the filter cartridge is in the snapped state, the culture medium can be added to the inside of the filter cartridge, which facilitates the filtration of insoluble substances contained in the culture medium through the filter cartridge. When the protrusion rotates in the can, it can contact the side wall of the filter cartridge. The vibration generated by the rotation of the protrusion can make the bottom of the filter cartridge shake, which can prevent the internal mesh of the filter cartridge from being blocked when using the filter cartridge, and thus improve the filtration effect of the filter cartridge. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the connection structure between the can lid and the motor of this utility model;

[0018] Figure 3for Figure 2 The diagram shown is an enlarged view of the structure of part A.

[0019] Figure 4 This is a schematic diagram of the connection structure between the retaining ring and the filter cartridge of this utility model;

[0020] Figure 5 This is a schematic diagram of the connection structure between the can lid and the locking block of this utility model;

[0021] Figure 6 for Figure 5 The diagram shown is an enlarged view of the structure of section B.

[0022] Figure 7 This is a schematic diagram of the connection structure between the rotating rod and the protrusion of this utility model;

[0023] Figure 8 for Figure 7 The diagram shows an enlarged view of section C.

[0024] In the diagram: 1. Tank body; 2. Support; 3. Tank lid; 4. Fixing mechanism; 401. Mounting block; 402. Rotating block; 403. Clamping rod; 404. Abutment block; 405. Clamping block; 5. Stirring mechanism; 501. Motor; 502. Rotating shaft; 503. Rotating rod; 504. Stirring rod; 6. Filtration mechanism; 601. Sealing cover; 602. Fixing ring; 603. Slot; 604. Clamping ring; 605. Pull rod; 606. Filter cartridge; 7. Protrusion. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] Please see Figures 1-8As shown, a liquid microbial culture propagation device includes a tank 1, which is connected to a tank cover 3 via a fixing mechanism 4. A stirring mechanism 5 and a filtering mechanism 6 are fixed on the tank cover 3. A protrusion 7 is installed on the stirring mechanism 5. The filtering mechanism 6 includes a sealing cap 601 and a fixing ring 602. The fixing ring 602 is fixedly installed on the tank cover 3. A groove 603 is provided in the fixing ring 602. A retaining ring 604 is engaged with the groove 603. The retaining ring 604 is engaged with the fixing ring 602. The height of the retaining ring 604 is less than the height of the groove 603. The sealing cap 601 is rotated open, and the retaining ring lever 605 is grasped to place the retaining ring 604 into the groove 603. Since the fixing ring 602 is installed at the top of the tank cover 3, it can limit the retaining ring 604. A pull rod 605 and a filter cartridge 606 are fixedly connected. The filter cartridge 606 is connected to the interior of the tank body 1 through the tank cover 3, and the bottom end of the filter cartridge 606 is set with an arc surface structure. The filter cartridge 606 is connected to the interior of the tank body 1 through the tank cover 3. The culture medium is added to the interior of the filter cartridge 606 to facilitate the filtration of insoluble substances contained in the culture medium through the filter cartridge 606. A sealing cap 601 is threaded on the side wall of the fixing ring 602. The sealing cap 601 contacts the top end of the tank cover 3. The protrusion 7 contacts the side wall of the filter cartridge 606. The part of the protrusion 7 that contacts the filter cartridge 606 is set with an arc surface structure. When the protrusion 7 rotates in the tank body 1, it can contact the side wall of the filter cartridge 606. The rotation of the protrusion 7 generates vibration, which can make the bottom end of the filter cartridge 606 shake. This prevents the internal mesh of the filter cartridge 606 from clogging when using the filter cartridge 606, which helps to improve the filtration effect of the filter cartridge 606.

[0027] As a technical optimization of this utility model, the stirring mechanism 5 includes a motor 501 and a rotating shaft 502. The motor 501 is fixedly installed on the top of the tank cover 3. The output end of the motor 501 is fixedly connected to the rotating shaft 502 through a coupling. The rotating shaft 502 is rotatably connected to the tank cover 3. Several rotating rods 503 and stirring rods 504 are fixedly installed on the side wall of the rotating shaft 502. When the motor 501 is turned on, the rotating shaft 502 drives the rotating rods 503 and stirring rods 504 to stir and mix the liquid bacteria in the tank 1, which helps to avoid the precipitation of nutrients and bacteria and ensures the uniformity of the culture medium composition. The protrusion 7 is fixedly connected to the rotating rod 503. The rotating rod 503 is arranged in an arc shape. Several rotating rods 503 and stirring rods 504 are arranged in a ring at equal intervals. When the rotating rod 503 rotates, it can drive the protrusion 7 to rotate in the tank 1.

[0028] As a technical optimization of this utility model, the fixing mechanism 4 includes a mounting block 401 and a rotating block 402. Several mounting blocks 401 are fixedly connected in a ring at equal intervals to the side wall of the tank body 1. A rotating block 402 is rotatably connected inside the mounting block 401. A locking rod 403 is fixedly connected to the rotating block 402, located at the top of the rotating block 402. The top of the locking rod 403 has a spherical structure. The tank lid 3 is snapped onto the top of the tank body 1. The groove in the locking block 405 is aligned with the rotating block 402. By gripping and rotating the locking rod 403, the locking rod 403 drives the rotating block 402 to move along the mounting block 401. The inner wall of mounting block 401 is rotated, so that the locking rod 403 and the locking block 405 are engaged. The locking rod 403 is threaded with a stop block 404, which is a regular hexagonal structure. The can lid 3 is fixedly connected with the locking block 405. The bottom end of the stop block 404 abuts against the locking block 405. The part of the locking block 405 that contacts the locking rod 403 is an arc-shaped structure. The stop block 404 is rotated with a tool. The stop block 404 rotates along the locking rod 403. When its bottom end abuts against the locking block 405, the locking block 405 can be fixed to the top of the mounting block 401, thereby facilitating the fixing of the can lid 3 to the top of the can body 1.

[0029] As a technical optimization of this utility model, a number of supports 2 are fixedly connected in a ring at equal intervals on the tank body 1. The supports 2 are located on the side wall at the bottom of the tank body 1, and the supports 2 support the tank body 1 so that the tank body 1 is in a relatively stable state during use.

[0030] In use, the operator first attaches the can lid 3 to the top of the can body 1, aligns the groove in the locking block 405 with the rotating block 402, holds the top of the locking rod 403, and rotates the locking rod 403. The locking rod 403 drives the rotating block 402 to rotate along the inner wall of the mounting block 401 fixed to the side wall of the can body 1. When the locking rod 403 is engaged with the locking block 405, a tool can be used to rotate the abutment block 404. The abutment block 404 rotates along the locking rod 403. When its bottom end abuts against the locking block 405, the locking block 405 can be fixed to the top of the mounting block 401, thus facilitating the fixing of the can lid 3 to the top of the can body 1. When using the can body 1, the control switch of the motor 501 is turned on, causing the rotating shaft 502 to drive the rotating rod 503 and the stirring rod 504 to stir and mix the liquid culture in the can body 1, which helps to avoid the precipitation of nutrients and bacteria and ensures the uniformity of the culture medium components. When it is necessary to add liquid culture medium to the can body 1... The operator can rotate to open the sealing cover 601 threaded on the side wall of the fixing ring 602, and then grasp the pull rod 605 fixed to the inner wall of the retaining ring 604, and put the retaining ring 604 into the retaining groove 603 provided in the fixing ring 602. Since the fixing ring 602 is installed at the top of the tank cover 3, the retaining ring 604 can be limited by the fixing ring 602. The bottom end of the retaining ring 604 is fixedly installed with a filter cartridge 606. The filter cartridge 606 is connected to the inside of the tank body 1 through the tank cover 3. The culture medium is added to the inside of the filter cartridge 606, which facilitates the filtration of insoluble substances contained in the culture medium through the filter cartridge 606. The protrusion 7 is fixed to the top of the rotating rod 503. When the rotating rod 503 drives the protrusion 7 to rotate in the tank body 1, it can contact the side wall of the filter cartridge 606. The rotation of the protrusion 7 generates vibration, which can make the bottom end of the filter cartridge 606 shake, thereby preventing the internal mesh of the filter cartridge 606 from being blocked when using the filter cartridge 606, which is beneficial to improving the filtration effect of the filter cartridge 606.

[0031] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0032] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A liquid seed propagation apparatus comprising a tank (1), characterised in that: The tank body (1) is connected with the tank cover (3) through the fixing mechanism (4), the tank cover (3) is fixed with the stirring mechanism (5) and the filtering mechanism (6), the stirring mechanism (5) is provided with the protruding block (7), the filtering mechanism (6) comprises a sealing cover (601) and a fixing ring (602), the fixing ring (602) is fixedly installed on the tank cover (3), the fixing ring (602) is provided with a clamping groove (603), the clamping groove (603) is provided with a clamping ring (604) in a clamping mode, the clamping ring (604) is fixedly connected with the pull rod (605) and the filter cartridge (606), the sealing cover (601) is threadedly installed on the side wall of the fixing ring (602), and the protruding block (7) is in contact with the side wall of the filter cartridge (606).

2. A liquid inoculum propagation apparatus according to claim 1, wherein: The clamping ring (604) is clamped and connected with the fixing ring (602), the sealing cover (601) is in contact with the top end of the tank cover (3), and the height of the clamping ring (604) is less than the height of the clamping groove (603).

3. A liquid inoculum propagation apparatus according to claim 1, wherein: The filter cartridge (606) is communicated with the inside of the tank body (1) through the tank cover (3), and the bottom end of the filter cartridge (606) is provided in an arc surface structure.

4. A liquid inoculum propagation apparatus according to claim 1, wherein: The stirring mechanism (5) comprises a motor (501) and a rotating shaft (502), the top end of the tank cover (3) is fixedly provided with the motor (501), the output end of the motor (501) is fixedly connected with the rotating shaft (502) through a shaft coupling, and the side wall of the rotating shaft (502) is fixedly provided with a plurality of rotating rods (503) and stirring rods (504).

5. A liquid inoculum propagation apparatus according to claim 4, wherein: The rotating rods (503) and the stirring rods (504) are arranged in a ring shape at equal intervals, and the protruding block (7) is fixedly connected with the rotating rods (503).

6. A liquid inoculum propagation apparatus according to claim 4, wherein: The rotating shaft (502) is rotatably connected with the tank cover (3), and the rotating rods (503) are provided in an arc structure.

7. A liquid inoculum propagation apparatus according to claim 1, wherein: The fixing mechanism (4) comprises a mounting block (401) and a rotating block (402), the side wall of the tank body (1) is fixedly connected with a plurality of mounting blocks (401) in a ring shape at equal intervals, the rotating block (402) is rotatably connected in the mounting block (401), the clamping rod (403) is fixedly connected on the rotating block (402), the resisting block (404) is threadedly connected on the clamping rod (403), the clamping block (405) is fixedly connected on the tank cover (3), and the clamping rod (403) is clamped and connected with the clamping block (405).

8. A liquid inoculum propagation apparatus according to claim 7, wherein: The clamping rod (403) is located at the top end of the rotating block (402), the top of the clamping rod (403) is provided in a spherical structure, and the resisting block (404) is provided in a regular hexagonal structure.

9. A liquid inoculum propagation apparatus according to claim 7, wherein: The part, in which the clamping block (405) is in contact with the clamping rod (403), is provided in an arc surface structure, and the bottom end of the resisting block (404) is in contact with the clamping block (405).

10. A liquid inoculum propagation apparatus according to claim 1, wherein: A plurality of brackets (2) are fixedly connected on the tank body (1) in a ring shape at equal intervals, and the brackets (2) are located on the side wall of the bottom of the tank body (1).

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