Biological sample collecting tube

Abstract

The utility model relates to a biological sample collecting tube which comprises a tube body and a piston body, two ends of the tube body are open, the piston body comprises a push-pull rod and a piston head, the piston head is arranged in a collecting cavity of the tube body and is in interference fit with the collecting cavity, one end of the push-pull rod is connected with the piston head, and the other end of the push-pull rod is connected with the piston head. The push-pull rod is used for driving the piston head to move in the axial direction of the collecting cavity, and a sample adsorption layer is arranged at the end, away from the push-pull rod, of the piston head. Compared with the prior art, according to the implementation of the technical scheme, the silica gel collecting surface can be quickly pushed out through the operation of the push-pull rod, and a sample is vertically observed under a microscope, so that the loss in the transfer process is avoided, and quick sampling and observation can be realized; the sample is located in the bottle body, the reagent utilization rate is improved, reagent loss is reduced, tool intervention and sample taking-out processes are simplified through the push-pull rod design, and the experiment efficiency is improved.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical technology, specifically relating to a biological sample collection tube. Background Technology

[0002] Organisms are composed of various cell types that are interconnected. Previously, studies using whole tissue samples (mixed samples) did not accurately reflect the biological information of individual cell types. Tissue sections, with advancements in microscopy and immunoassay, have addressed the need for obtaining some tissue samples at a single level. However, this goal remains difficult to achieve due to mechanical and procedural limitations in cell manipulation (e.g., cell collection or storage) and the considerable time required. In particular, isolating single or rare cells or microscopic tissues from tiny samples requires researchers to capture individual cells under a microscope; therefore, ensuring that the sample container contains only one cell for further testing is crucial.

[0003] Existing biological sample collection techniques (such as gravity sampling and blow sampling) suffer from problems such as complex operation, easy sample loss, and limited reagent addition, especially with low collection efficiency for single-cell or small tissue samples. Traditional methods rely on storing trace amounts of reagent in the container cap, resulting in high reagent loss rates and failing to achieve rapid sampling and observation.

[0004] In summary, there is an urgent need to provide a biological sample collection tube that can improve experimental efficiency and reduce sample loss and reagent waste. Utility Model Content

[0005] The purpose of this invention is to provide a biological sample collection tube that can improve experimental efficiency and reduce sample loss and reagent waste.

[0006] The above objective is achieved through the following technical solution: a biological sample collection tube, comprising a tube body and a piston body, wherein the tube body is open at both ends, the piston body comprises a push-pull rod and a piston head, the piston head is disposed in the collection cavity of the tube body and is interference-fitted with the collection cavity, one end of the push-pull rod is connected to the piston head, and the other end extends out of the collection cavity, the push-pull rod is used to drive the piston head to move axially along the collection cavity, and a sample adsorption layer is provided at the end of the piston head away from the push-pull rod.

[0007] This invention is used for biological samples. In specific applications, when biological samples observed under a microscope need to be immediately removed and transferred to other containers for subsequent testing, the piston head is pushed out using a push-pull rod to remove the sample adsorption layer collection surface on the piston head. After obtaining the sample, the piston head is pulled back using the push-pull rod, and reagent is added. The piston head and collection chamber are interference-fitted, and because of the support of the piston wall, the amount of reagent can be increased without overflow, allowing for rapid mixing and sampling using a sampling gun. Secondly, the piston-type sample collection allows for a simpler way to move an operating tool with an operating tip into the sample container and then remove it. Furthermore, the collection tube can be used directly as a reaction vessel. Since the sample is placed inside the collection chamber of the reaction vessel, a conventional amount (ml) of reagent can be used for further reactions, further reducing the loss rate compared to the existing technology where trace amounts of reagent are stored in the lid of the reaction vessel (only a maximum of 10 μL of reagent can be added).

[0008] A further technical solution is that the sample adsorption layer is made of transparent silica gel, which is then encapsulated in the piston head. Using transparent silica gel helps maintain the integrity and visibility of the sample during collection, especially during microscopic observation. The transparent silica gel has a Shore hardness of 5, a coefficient of thermal expansion of 220 PPM / ℃, a thermal conductivity of 0.23 W / (m·K), and an effective temperature range of -60℃ to 220℃.

[0009] A further technical solution is to provide a PET or POL film on the transparent silicone. The PET or POL film on the transparent silicone, through physical isolation, optical optimization, chemical protection, and biosafety design, significantly improves the performance of the piston-type biological sample collection tube, enabling it to efficiently and non-destructively collect, observe, and transfer tiny samples, while also ensuring ease of operation and device durability.

[0010] A further technical solution is that the thickness of the PET or POL film is 1 to 2 micrometers. The ultra-thin design of 1-2 micrometers ensures mechanical strength while minimizing interference with the optical path, making it suitable for the observation needs of high-resolution microscopes (such as confocal microscopes).

[0011] A further technical solution is that the tube is cylindrical. Cylindrical biological sample collection tubes can be held upright under a microscope for sample observation.

[0012] A further technical solution is that the end of the push-pull rod is provided with a handle or anti-slip texture. The handle or anti-slip texture at the end of the push-pull rod allows for deflection during operation and is suitable for positioning micron-sized samples under a microscope.

[0013] A further technical solution is that the outer wall of the tube is marked with graduation lines. The graduation unit can be microliters or milliliters, set according to actual conditions, for quantitative reagent addition or sample volume measurement; the graduation lines provide intuitive volume indication, meeting the needs of precise control of reagent addition and sample volume in experiments.

[0014] Compared to existing technologies, the implementation of this utility model allows for rapid expansion of the silica gel collection surface via a push-pull rod, enabling upright observation of the sample under a microscope and avoiding losses during the transfer process. This facilitates rapid sampling and observation. The sample is located inside the vial, supporting the addition of conventional amounts (milliliters) of reagents. Compared to traditional micro-droplet addition (≤10 μL), reagent utilization is improved, and reagent loss is reduced. The push-pull rod design simplifies the tool intervention and sample removal process, enhancing experimental efficiency. It is suitable for separating single or rare cells or small case tissues from tiny samples, and the transparency of the silica gel ensures clear observation under a microscope.

[0015] This invention solves the problems of complex operation, fragile samples, and waste of reagents in traditional biological sample collection technology, and significantly improves the efficiency, safety and multi-scenario compatibility of small sample processing. Attached Figure Description

[0016] The accompanying drawings, which form part of this utility model, are used to provide a further understanding of this utility model. The illustrative embodiments of this utility model and their descriptions are used to explain this utility model and do not constitute an improper limitation of this utility model.

[0017] Figure 1 This is a schematic diagram of the structure of a biological sample collection tube according to one embodiment of the present invention;

[0018] Figure 2 for Figure 1 A cross-sectional schematic diagram of the biological sample collection tube before the sample adsorption layer is pushed out;

[0019] Figure 3 for Figure 1 The diagram shows a cross-sectional view of the sample adsorption layer being pushed out of the biological sample collection tube involved in the study.

[0020] In the picture:

[0021] 1. Transparent silicone 2. Tube body 3. Piston head 4. Push-pull rod

[0022] 5 POL film 6 handle 7 collection chamber Detailed Implementation

[0023] The present invention will now be described in detail with reference to the accompanying drawings. This description is merely illustrative and explanatory, and should not be construed as limiting the scope of protection of the present invention. Furthermore, those skilled in the art can combine the features in the embodiments described herein and in different embodiments according to the description in this document.

[0024] The embodiments of this utility model are as follows, please refer to... Figures 1-3 A biological sample collection tube includes a tube body 2 and a piston body. The tube body 2 is open at both ends. The piston body includes a push-pull rod 4 and a piston head 3. The piston head 3 is disposed in the collection cavity 7 of the tube body 2 and is interference-fitted with the collection cavity 7. One end of the push-pull rod 4 is connected to the piston head 3, and the other end extends out of the collection cavity 7. The push-pull rod 4 is used to drive the piston head 3 to move axially along the collection cavity 7. A sample adsorption layer is provided at the end of the piston head 3 away from the push-pull rod 4.

[0025] This invention is used for biological samples. In specific applications, the volume of the collection tube 2 can be 500 μL, 1 mL, 1.5 mL, or 2 mL, with a specific embodiment using 1 mL. When a biological sample observed under a microscope needs to be immediately removed and transferred to another container for subsequent testing, the piston head 3 is pushed out using the push-pull rod 4 to remove the sample adsorption layer collection surface on the piston head 3. After obtaining the sample, the piston head 3 is pulled back using the push-pull rod 4, and reagent is added. The piston head 3 is interference-fitted with the collection chamber 7. Because of the support of the piston wall, the amount of reagent can be increased without overflow, allowing for rapid mixing and sampling using a sampling gun. Secondly, the piston-type sample collection allows for a simpler way to move an operating tool with an operating tip into the sample container and then remove it. Furthermore, the collection tube can be used directly as a reaction vessel. Since the sample is placed inside the collection chamber 7 of the reaction vessel, a conventional amount (mL) of reagent can be used for further reactions. Compared to the existing technology where trace amounts of reagent are stored in the lid of the reaction vessel (only a maximum of 10 μL of reagent can be added), the loss rate is further reduced.

[0026] Based on the above embodiments, in another embodiment of the present invention, such as Figure 2 and Figure 3 The sample adsorption layer is transparent silica gel 1, which is encapsulated on the piston head 3. Using transparent silica gel 1 helps maintain the integrity and visibility of the sample during collection, especially during microscopic observation. The transparent silica gel 1 has a Shore hardness of 5, a coefficient of thermal expansion of 220 PPM / ℃, a thermal conductivity of 0.23 W / (m·K), and an effective temperature range of -60℃ to 220℃.

[0027] Based on the above embodiments, in another embodiment of the present invention, such as Figure 2 and Figure 3 The transparent silicone 1 is provided with a PET or POL film 5. In a specific embodiment, a POL film 5 may be used; the transparent silicone 1 is provided with a PET or POL film 5, which significantly improves the performance of the piston-type biological sample collection tube through physical isolation, optical optimization, chemical protection and biosafety design, so that it can efficiently and non-destructively complete the collection, observation and transfer of small samples, while taking into account the convenience of operation and the durability of the device.

[0028] Based on the above embodiments, in another embodiment of the present invention, such as Figure 2 and Figure 3 The thickness of the PET or POL film 5 is 1 to 2 micrometers. In a specific embodiment, the film thickness is 1.5 micrometers. The ultra-thin design of 1-2 micrometers ensures mechanical strength while minimizing interference with the optical path, making it suitable for the observation needs of high-resolution microscopes (such as confocal microscopes).

[0029] Based on the above embodiments, in another embodiment of the present invention, such as Figure 1 The tube body 2 is cylindrical. The inner diameter of the tube body 2 can be set to 1 cm. The cylindrical biological sample collection tube can be placed upright under a microscope for sample observation.

[0030] Based on the above embodiments, in another embodiment of the present invention, such as Figure 1 The push-pull rod 4 has a handle 6 or anti-slip texture at its end. The handle 6 or anti-slip texture at the end of the push-pull rod 4 allows for easy operation and is suitable for positioning micron-sized samples under a microscope.

[0031] Based on the above embodiments, in another embodiment of this utility model, the outer wall of the tube 2 is marked with graduation lines. The graduation unit can be microliters or milliliters, set according to actual conditions, for quantitative reagent addition or sample volume measurement; the graduation lines provide intuitive volume indication, meeting the precise control requirements for reagent addition and sample volume in experiments.

[0032] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A biological sample collection tube, characterized by, The application relates to a sample collection device, which comprises a tube body and a piston body, the tube body being open at both ends, the piston body comprising a push-pull rod and a piston head, the piston head being arranged in a collection cavity of the tube body and being in interference fit with the collection cavity, one end of the push-pull rod being connected with the piston head, the other end of the push-pull rod extending out of the collection cavity, the push-pull rod being used to drive the piston head to move in the axial direction of the collection cavity, and a sample adsorption layer being arranged on the end of the piston head away from the push-pull rod.

2. The biological sample collection tube of claim 1, wherein, The sample adsorption layer is transparent silica gel, and the transparent silica gel is filled in the piston head.

3. The biological sample collection tube of claim 2, wherein, A PET or POL film is arranged on the transparent silica gel.

4. The biological sample collection tube of claim 3, wherein, The thickness of the PET or POL film is 1-2 microns.

5. The biological sample collection tube of claim 3, wherein, The tube body is cylindrical.

6. The biological sample collection tube according to any one of claims 1 to 5, wherein, A knob or anti-skid pattern is arranged on the end of the push-pull rod.

7. The biological sample collection tube according to any one of claims 1 to 5, wherein, Scale lines are marked on the outer wall of the tube body.

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