Soil sediment DNA extraction device with temperature regulation and control function

Abstract

The utility model discloses a soil sediment DNA (deoxyribonucleic acid) extraction device with a temperature regulation and control function, which belongs to the technical field of sediment extraction and comprises an arc-shaped frame, fixed end plates are symmetrically and fixedly mounted at two ends of the arc-shaped frame, a mounting seat is fixedly mounted on one side of one fixed end plate, and the other side of the other fixed end plate is provided with a temperature regulation and control device. And a stirring motor is fixedly mounted in the mounting seat in an embedded manner. According to the soil sediment DNA extraction device with the temperature regulation and control function, a heating plate, a stirring motor, a stirring shaft and stirring blades are arranged, and the stirring motor and the heating plate are started at the same time, so that the stirring motor drives the stirring shaft to rotate in an arc-shaped frame, and the stirring blades stir between the arc-shaped frame and an arc-shaped filter screen; meanwhile, the heating plate is used for heating the soil sediments in the arc-shaped frame, so that the extraction of DNA in the soil sediments is accelerated, and impurities in the soil sediments are prevented from blocking the arc-shaped filter screen to influence the filtration and extraction of the DNA by the arc-shaped filter screen.

Description

Technical Field

[0001] This invention belongs to the field of sediment extraction technology, specifically a soil sediment DNA extraction device with temperature control function. Background Technology

[0002] Soil sediments are complex environmental samples containing rich microbial communities. These microorganisms play a vital role in the material cycling, energy conversion, and maintenance of ecological balance in soil ecosystems. Extracting and analyzing DNA from soil sediments can quickly and accurately identify the species of plants, animals, and microorganisms in the soil, which helps to understand the species composition and diversity of the ecosystem.

[0003] Existing soil sediment DNA extraction devices suffer from solvent settling and clogging of the filter screen during filtration extraction, making it difficult to extract DNA from the soil sediment. Therefore, improvements are needed. Utility Model Content

[0004] To overcome the above-mentioned defects, this utility model provides a soil sediment DNA extraction device with temperature control function, which solves the problem that in existing soil sediment DNA extraction devices, the soil sediment solvent settles and clogs the filter screen during filtration extraction, making it difficult to filter and extract DNA from the soil sediment.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a soil sediment DNA extraction device with temperature control function, comprising an arc-shaped frame, with fixed end plates symmetrically fixed at both ends of the arc-shaped frame, a mounting base fixedly installed on one side of one of the fixed end plates, a stirring motor embedded and fixedly installed inside the mounting base, a stirring shaft rotatably installed between the two fixed end plates at the bottom of the arc-shaped frame, a plurality of stirring blades uniformly fixedly installed on the surface of the stirring shaft between the two fixed end plates, one end of the stirring shaft being fixedly connected to the output end of the stirring motor, and a liquid collection box provided at the bottom of the arc-shaped frame.

[0006] As a further embodiment of this utility model: fixing strips are symmetrically fixedly installed on both sides of the arc-shaped frame, and a rubber pad is fixedly installed at the center of the top of the fixing strip.

[0007] As a further embodiment of this utility model: two fixing blocks are symmetrically fixedly installed on both sides of the liquid collection box, and an L-shaped card plate is rotatably installed between the two opposing fixing blocks, with the L-shaped card plate corresponding to the position of the rubber pad.

[0008] As a further embodiment of this utility model: a heating plate is fixedly installed at the top center of the surface of the arc-shaped frame, and an arc-shaped filter screen is provided between the arc-shaped frame and the liquid collection box.

[0009] As a further embodiment of this utility model: the liquid collection box is provided with symmetrical arc-shaped slots at both ends, and the arc-shaped slots have the same shape as the arc-shaped filter screen.

[0010] As a further embodiment of this utility model: positioning plates are fixedly installed at both ends of the arc-shaped filter screen, and the positioning plates are located on the outer side of both ends of the liquid collection box.

[0011] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0012] 1. This soil sediment DNA extraction device with temperature control function, by setting up a heating plate, stirring motor, stirring shaft and stirring blades, places the soil sediment inside the arc-shaped frame, assembles the arc-shaped frame, arc-shaped filter and collection box, and starts the stirring motor and heating plate at the same time. The stirring motor drives the stirring shaft to rotate inside the arc-shaped frame, and the stirring blades stir between the arc-shaped frame and the arc-shaped filter. At the same time, the heating plate heats the soil sediment inside the arc-shaped frame, thereby accelerating the extraction of DNA from the soil sediment, and preventing impurities in the soil sediment from clogging the arc-shaped filter and affecting the filtering and extraction of DNA by the arc-shaped filter.

[0013] 2. This soil sediment DNA extraction device with temperature control function, by setting up an arc-shaped filter, a collection box, a fixing strip, and an L-shaped plate, after the DNA extraction of soil sediment inside the arc frame is completed, rotate the L-shaped plate to disengage from the fixing strip, remove the collection box from the bottom of the arc frame, and at the same time move the arc-shaped filter away from the arc frame along with the collection box, thereby facilitating the cleaning and replacement of the arc-shaped filter and the cleaning of the inner wall of the arc frame. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model;

[0015] Figure 2 This is a cross-sectional view of the arc-shaped frame and mounting base of this utility model;

[0016] Figure 3 This is a schematic diagram of the liquid collection box and L-shaped card plate of this utility model;

[0017] Figure 4 This is a schematic diagram of the structure of the arc-shaped filter screen and positioning plate of this utility model;

[0018] In the diagram: 1. Arc-shaped frame; 2. Heating plate; 3. Fixed end plate; 4. Fixed clip; 5. Rubber pad; 6. Mounting base; 7. Stirring motor; 8. Stirring shaft; 9. Stirring blades; 10. Liquid collection box; 11. Fixing block; 12. L-shaped clamping plate; 13. Arc-shaped groove; 14. Arc-shaped filter screen; 15. Positioning clamping plate. Detailed Implementation

[0019] The technical solution of this patent will be further described in detail below with reference to specific embodiments.

[0020] like Figure 1-4 As shown, this utility model provides a technical solution: a soil sediment DNA extraction device with temperature control function, including an arc-shaped frame 1, with fixing strips 4 symmetrically fixed on both sides of the arc-shaped frame 1, and a rubber pad 5 fixedly installed at the center of the top of the fixing strips 4. When the L-shaped plate 12 is clamped on the top of the rubber pad 5, the rubber pad 5 is squeezed and deformed, thereby clamping and fixing the L-shaped plate 12, ensuring that the liquid collection box 10 can be clamped and fixed at the bottom of the arc-shaped frame 1.

[0021] The arc-shaped frame 1 has fixed end plates 3 symmetrically fixed at both ends. One of the fixed end plates 3 has a mounting base 6 fixedly fixed on one side. The mounting base 6 is embedded and fixedly installed with a stirring motor 7. The stirring shaft 8 is rotatably installed between the two fixed end plates 3 at the bottom of the arc-shaped frame 1. Multiple stirring blades 9 are evenly fixedly installed on the surface of the stirring shaft 8 between the two fixed end plates 3. One end of the stirring shaft 8 is fixedly connected to the output end of the stirring motor 7. Because of the stirring motor 7, after the arc-shaped frame 1, the arc-shaped filter screen 14 and the liquid collection box 10 are assembled, the stirring motor 7 is started to drive the stirring shaft 8 and the stirring blades 9 to rotate between the arc-shaped frame 1 and the arc-shaped filter screen 14. This allows the stirring blades 9 to stir the soil sediment, accelerate the extraction of DNA from the soil sediment, and at the same time prevent the soil sediment from clogging the arc-shaped filter screen 14, which would affect the extraction of DNA from the soil sediment.

[0022] The bottom of the arc-shaped frame 1 is provided with a liquid collection box 10. Two fixing blocks 11 are symmetrically fixed on both sides of the liquid collection box 10. An L-shaped clamping plate 12 is rotatably installed between the two opposing fixing blocks 11. The L-shaped clamping plate 12 corresponds to the position of the rubber clamping pad 5. By providing the L-shaped clamping plate 12, after the liquid collection box 10 is correspondingly clamped at the bottom of the arc-shaped frame 1, the L-shaped clamping plate 12 is rotated to clamp the top of the corresponding fixing strip 4, thereby facilitating the combination and installation of the arc-shaped frame 1 and the liquid collection box 10.

[0023] A heating plate 2 is fixedly installed at the top center of the surface of the arc-shaped frame 1. An arc-shaped filter screen 14 is provided between the arc-shaped frame 1 and the liquid collection box 10. Because the heating plate 2 is provided, the heating plate 2 is activated to heat the bottom of the arc-shaped frame 1, thereby accelerating the extraction of DNA from the soil sediment at the bottom of the arc-shaped frame 1.

[0024] The collection box 10 has symmetrical arc-shaped slots 13 at both ends, and the arc-shaped slots 13 are the same shape as the arc-shaped filter screen 14. Positioning plates 15 are fixedly installed at both ends of the arc-shaped filter screen 14, and the positioning plates 15 are located on the outer sides of both ends of the collection box 10. By providing the positioning plates 15, when the arc-shaped filter screen 14 is placed on top of the collection box 10, it is ensured that the arc-shaped filter screen 14 is tightly fitted inside the arc-shaped slots 13, and the positioning plates 15 are tightly fitted at both ends of the collection box 10, thus ensuring that the arc-shaped filter screen 14 will not be misaligned or shifted when it is secured to the top of the collection box 10.

[0025] The working principle of this utility model is as follows:

[0026] Invert the arc-shaped frame 1 and pour an appropriate amount of soil sediment into it. Then, place the arc-shaped filter screen 14 and the collection box 10 on the surface of the arc-shaped frame 1 in sequence. Rotate the L-shaped clamping plate 12 to fit it tightly with the rubber clamping pad 5, thereby assembling and installing the arc-shaped frame 1, the arc-shaped filter screen 14, and the collection box 10. Flip the arc-shaped frame 1 over and place the collection box 10 on a horizontal surface. Start the heating plate 2 and the stirring motor 7. The heating plate 2 heats the soil sediment between the arc-shaped frame 1 and the arc-shaped filter screen 14. The stirring motor 7 controls the stirring blades 9 to rotate between the arc-shaped frame 1 and the arc-shaped filter screen 14, thereby increasing the speed of DNA extraction from the soil sediment and preventing the soil sediment from clogging the arc-shaped filter screen 14. The filtered DNA extract falls into the collection box 10 for centralized processing.

[0027] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0028] The preferred embodiments of this patent have been described in detail above. However, this patent is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this patent.

Claims

1. A soil sediment DNA extraction device with temperature regulation function, comprising an arc-shaped frame (1), characterized in that: Both ends of the arc-shaped frame (1) are symmetrically fixedly installed with fixed end plates (3), one side of one of the fixed end plates (3) is fixedly installed with a mounting seat (6), the mounting seat (6) is fixedly installed with a stirring motor (7) in the inside, a stirring shaft (8) is rotatably installed between the two fixed end plates (3) at the bottom of the arc-shaped frame (1), a plurality of stirring blades (9) are uniformly fixedly installed on the surface of the stirring shaft (8) between the two fixed end plates (3), one end of the stirring shaft (8) is fixedly connected with the output end of the stirring motor (7), and the bottom of the arc-shaped frame (1) is provided with a liquid collecting box (10).

2. The soil sediment DNA extraction device with temperature regulation function according to claim 1, characterized in that: Both sides of the arc-shaped frame (1) are symmetrically fixedly installed with fixed clamping strips (4), and rubber clamping pads (5) are fixedly installed at the central positions of the top portions of the fixed clamping strips (4).

3. The soil sediment DNA extraction device with temperature regulation function according to claim 2, characterized in that: Two fixed blocks (11) are symmetrically fixedly installed at the two sides of the liquid collecting box (10), an L-shaped clamping plate (12) is rotatably installed between the two opposite fixed blocks (11), and the L-shaped clamping plate (12) corresponds in position to the rubber clamping pad (5).

4. The soil sediment DNA extraction device with temperature regulation function according to claim 1, characterized in that: A heating plate (2) is fixedly installed at the top central position of the surface of the arc-shaped frame (1), and an arc-shaped filter screen (14) is arranged between the arc-shaped frame (1) and the liquid collecting box (10).

5. The soil sediment DNA extraction device with temperature regulation function according to claim 4, characterized in that: Arc-shaped clamping grooves (13) are symmetrically arranged at the two ends of the liquid collecting box (10), and the arc-shaped clamping grooves (13) are the same in shape as the arc-shaped filter screen (14).

6. The soil sediment DNA extraction device with temperature regulation function according to claim 5, characterized in that: Positioning clamping plates (15) are fixedly installed at the two ends of the arc-shaped filter screen (14), and the positioning clamping plates (15) are located outside the two ends of the liquid collecting box (10).

Images