Culture device

Abstract

The utility model discloses a culture device which comprises a liquid containing plate, a cell culture plate and a cover plate, the cell culture plate is installed on the liquid containing plate, the cover plate covers the cell culture plate and the liquid containing plate, a first ventilation gap is formed between the cover plate and the liquid containing plate, and a second ventilation gap is formed between the cover plate and the liquid containing plate. The cell culture plate comprises a plate body and a plurality of culture parts arranged on the plate body at intervals, the liquid containing plate is provided with a liquid containing part used for containing the culture parts, each culture part is provided with a culture cavity and a plurality of liquid inlet channels, and the liquid inlet channels are formed in the groove wall of the culture cavity so as to communicate the culture cavity with the liquid containing part. According to the utility model, a plurality of liquid inlet channels are arranged in the culture cavity, so that the problem that the flow speed is too high in the rapid liquid supplementing process is solved, the liquid can be rapidly replaced in the culture process, and the culture liquid in the liquid containing cavities can be replaced or supplemented at one time by lifting the cell culture plate and replacing the culture liquid in the liquid containing plate.

Description

Technical Field

[0001] This utility model relates to the field of artificial skin cell culture technology, and in particular to a culture device. Background Technology

[0002] The cultivation of artificial skin cells has significant applications in various industries, including medicine and cosmetics. However, the cultivation process is complex and time-consuming, representing a sophisticated technology spanning multiple fields such as bioengineering and materials science. Traditional artificial skin cell cultivation methods rely on conventional consumables such as culture dishes, plates, and flasks. These tools are simple to operate and suitable for small-scale cell culture experiments in the laboratory. However, when it comes to actual artificial skin cell cultivation, especially when rapid fluid resuscitation is required, traditional cultivation tools cannot meet the demands. Utility Model Content

[0003] The technical problem this invention aims to solve is that traditional culture tools cannot meet the need for rapid fluid replenishment in artificial skin cell culture.

[0004] To address the aforementioned technical problems, this utility model provides a culture device, comprising a liquid-filling plate, a cell culture plate, and a cover plate. The cell culture plate is mounted on the liquid-filling plate, and the cover plate covers both the cell culture plate and the liquid-filling plate, with a first air-permeable gap formed between the cover plate and the liquid-filling plate. The cell culture plate includes a plate body and a plurality of culture portions spaced apart on the plate body. The liquid-filling plate has a liquid-filling portion for placing the culture portions. The culture portions have a culture chamber and a plurality of liquid inlet channels. The plurality of liquid inlet channels are formed in the wall of the culture chamber to connect the culture chamber and the liquid-filling portion.

[0005] Furthermore, the plate body is provided with a plurality of first protrusions on the side facing the liquid-filling plate. The plurality of first protrusions are spaced apart around the axis of the corresponding culture part on the periphery of the culture part, so that when the culture part is installed on the liquid-filling part, a second air-permeable gap is formed between the plate body and the liquid-filling plate, and the second air-permeable gap communicates with the first air-permeable gap.

[0006] Furthermore, the liquid inlet channel includes liquid inlet holes and liquid inlet grooves, with a plurality of liquid inlet holes spaced apart around the axis of the culture chamber on the bottom wall of the culture chamber, and a plurality of liquid inlet grooves spaced apart on the side wall of the culture chamber.

[0007] Furthermore, the liquid-filling part is provided with a plurality of first limiting protrusions on the side opposite to the cell culture plate, and the cover plate is provided with a plurality of second limiting protrusions on the side opposite to the cell culture plate. The first limiting protrusions and the second limiting protrusions are adapted to each other, and the assembly gap between the first limiting protrusions and the second limiting protrusions is 0.5-1.5mm.

[0008] Furthermore, the liquid-filling plate includes a main body and a handle, with two handles disposed opposite to each other on both sides of the main body. The liquid-filling part is disposed on the main body. The liquid-filling plate also includes a plurality of reinforcing ribs, which are provided between the main body and the liquid-filling part, as well as between two adjacent liquid-filling parts.

[0009] Furthermore, a plurality of second protrusions are provided on the side of the main body away from the cell culture plate, and the plurality of second protrusions are provided at the four corners of the main body, and both ends of the second protrusions are provided with inclined surfaces.

[0010] Furthermore, the main body is provided with a first oblique angle structure, the plate has a first side and a second oblique angle structure, the second oblique angle structure is provided on the first side and is adapted to the first oblique angle structure, and the cover plate has a third oblique angle structure, the third oblique angle structure is adapted to the first oblique angle structure and the second oblique angle structure.

[0011] Furthermore, the plate body includes multiple support strips and support rings, and multiple culture portions are disposed within the support rings. The support rings and culture portions are connected by the support strips. The support rings have a first side and a second side disposed opposite to the first side. The distance between the support rings near the first side and the culture portions is L1, and the distance between the support rings near the second side and the culture portions is L2, where L1 = 2L2.

[0012] Furthermore, the culture section has a third limiting ridge on the side facing the bottom wall of the liquid-filling section.

[0013] Furthermore, the cover plate includes a cover body, multiple flange structures, and a third protrusion. The flange structures are spaced apart on the side of the cover body facing the culture section. The flange structures are adapted to the culture section and are arranged in a one-to-one correspondence with the culture section. The third protrusion is installed on the side of the cover body facing the culture section so that when the cover plate is placed on the cell culture plate, a third air-permeable gap is formed between the cover plate and the plate body. The third air-permeable gap communicates with the first air-permeable gap.

[0014] Compared with the prior art, the cultivation device of this utility model has the following advantages:

[0015] This utility model embodiment solves the problem of excessive flow rate that may occur during rapid replenishment by setting multiple liquid inlet channels in the culture chamber. It overcomes the problem that traditional culture tools cannot meet the rapid replenishment needs of artificial skin cell culture, and enables rapid liquid replacement during the culture process. By lifting the cell culture plate and replacing the culture medium in the liquid-filling plate, the culture medium in multiple liquid-filling chambers can be replaced or replenished at one time. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of the culture device provided in this embodiment of the utility model;

[0017] Figure 2 This is an exploded view of the culture apparatus provided in an embodiment of the present invention;

[0018] Figure 3 This is a cross-sectional view of the stacked culture devices provided in this embodiment of the present invention;

[0019] Figure 4 This is provided by the embodiment of the present utility model. Figure 3 A magnified view of part A circled in the diagram;

[0020] Figure 5 This is a schematic diagram of the liquid-filling plate at a first angle provided in an embodiment of the present invention;

[0021] Figure 6 This is a schematic diagram of the liquid-filling plate from a second angle provided in an embodiment of the present invention;

[0022] Figure 7 This is provided by the embodiment of the present utility model. Figure 6 A magnified view of part B circled in the diagram;

[0023] Figure 8 This is a schematic diagram of the cell culture plate provided in an embodiment of the present invention from a first angle.

[0024] Figure 9 This is a schematic diagram of the cell culture plate provided in this embodiment of the present invention from a second angle.

[0025] Figure 10 This is a structural schematic diagram of the cover plate at a first angle provided in an embodiment of the present utility model;

[0026] Figure 11 This is a structural schematic diagram of the cover plate at the second angle provided in an embodiment of the present utility model;

[0027] Figure 12 This is a schematic diagram of the culture state of the culture device provided in this embodiment of the utility model. The 12 culture chambers on the right are the state of skin cells cultured for about 7 days, and the 12 culture chambers on the left are the state of skin cells cultured for about 15 days.

[0028] In the diagram, 1. Liquid-filling plate; 11. Liquid-filling section; 12. First limiting protrusion; 13. Main body; 14. Handle; 15. Reinforcing rib; 16. Second protrusion; 17. Inclined surface; 18. First oblique angle structure; 2. Cell culture plate; 21. Plate body; 211. First side; 212. Second oblique angle structure; 213. Support strip; 214. Support ring; 215. Second side; 22. Culture section; 221. Culture chamber; 222. Liquid inlet channel; 2221. Liquid inlet hole; 2222. Liquid inlet groove; 23. First protrusion; 24. Third limiting protrusion; 3. Cover plate; 31. Second limiting protrusion; 32. Third oblique angle structure; 33. Cover body; 34. Flange structure; 35. Third protrusion; 4. First venting gap; 5. Second venting gap; 6. Third venting gap. Detailed Implementation

[0029] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit its scope.

[0030] like Figures 1 to 4 ,and Figure 8 As shown, this utility model provides a culture device, including a liquid-filling plate 1, a cell culture plate 2, and a cover plate 3. The cell culture plate 2 is installed on the liquid-filling plate 1, and the cover plate 3 covers the cell culture plate 2 and the liquid-filling plate 1, with a first air gap 4 formed between the cover plate 3 and the liquid-filling plate 1 to allow air to pass through. The cell culture plate 2 includes a plate body 21 and a plurality of culture sections 22 spaced apart on the plate body 21. The liquid-filling plate 1 has a liquid-filling section 11 for placing the culture sections 22. The culture section 22 has a culture chamber 221 and a plurality of liquid inlet channels 222. The culture chamber 221 is used to directly place skin cells. The plurality of liquid inlet channels 222 are opened in the groove wall of the culture chamber 221 to connect the culture chamber 221 and the liquid-filling section 11, so that the liquid can enter the culture chamber 221 at an appropriate flow rate, which not only ensures the need for rapid replenishment of liquid, but also avoids the impact on the skin cells caused by excessive flow rate.

[0031] like Figure 4 and Figure 9 As shown, the plate 21 has a plurality of first protrusions 23 on the side facing the liquid-filling plate 1. The plurality of first protrusions 23 are spaced around the axis of the corresponding culture section 22 and are arranged on the periphery of the culture section 22 so that when the culture section 22 is installed on the liquid-filling section 11, a second air-permeable gap 5 is formed between the plate 21 and the liquid-filling plate 1. The second air-permeable gap 5 is connected to the first air-permeable gap 4, which further enhances the gas circulation effect and ensures more sufficient gas exchange in the cell culture environment.

[0032] This embodiment solves the problem of excessive flow rate during rapid fluid replenishment by setting multiple liquid inlet channels 222 in the culture chamber 221. Simultaneously, by setting a double-layered venting gap, sufficient gas exchange is ensured, overcoming the limitation of traditional culture tools in meeting the rapid fluid replenishment requirements of artificial skin cell culture. Understandably, due to the presence of the second venting gap 5, the cell culture plate 2, when placed on the liquid-filling plate 1, protrudes 4mm around its perimeter, facilitating the removal of the cell culture plate 2 and enabling rapid medium replacement during culture. By lifting the cell culture plate 2 and replacing the culture medium in the liquid-filling plate 1, the culture medium in multiple liquid-filling chambers can be replaced or replenished at once.

[0033] In practical applications, the cell culture plate 2 is made of PS material that meets the USP Class VI standard. The material does not contain any added reagents and will not precipitate, so it will not affect cell growth. The cell culture plate 2 adopts a 2*6-chamber arrangement. Each liquid-filled plate 1 can hold two cell culture plates 2, and 24 culture chambers 221 of skin cells can be cultured at the same time. During the culture process, the medium can be changed quickly. The cell culture plate 2 can be lifted and the culture medium in the liquid-filled plate 1 can be replaced at one time, thus changing the culture medium in 24 culture chambers 221 at one time.

[0034] like Figure 9 As shown, the liquid inlet channel 222 includes liquid inlet holes 2221 and liquid inlet grooves 2222. Multiple liquid inlet holes 2221 are spaced apart around the axis of the culture chamber 221 on the bottom wall of the culture chamber 221. This ensures that the nutrient solution or other liquids required for culture are evenly distributed throughout the culture chamber 221, avoiding uneven cell growth caused by excessive or insufficient liquid supply in local areas. Multiple liquid inlet grooves 2222 are spaced apart on the side wall of the culture chamber 221 and work in conjunction with the liquid inlet holes 2221 to further regulate the speed and direction of liquid inflow. The presence of the liquid inlet grooves 2222 allows the liquid to flow more smoothly and evenly into the culture chamber 221, reducing the direct impact on the cells and also contributing to a more stable culture environment.

[0035] It should be noted that each culture chamber 221 has 5 liquid inlet holes 2221 with a diameter of 3mm at the bottom, and each culture chamber 221 has two liquid inlet grooves 2222 on the side. The liquid inlet grooves 2222 are 3mm wide and 25mm long.

[0036] like Figure 6 and Figure 10As shown, the liquid-filling part 11 is provided with a plurality of first limiting protrusions 12 on the side opposite to the cell culture plate 2, that is, the bottom of the liquid-filling part 11, and the cover plate 3 is provided with a plurality of second limiting protrusions 31 on the side opposite to the cell culture plate 2, that is, the top of the cover plate 3; the first limiting protrusions 12 and the second limiting protrusions 31 are adapted to each other so that they can fit together, thereby ensuring that multiple culture devices can be stably stacked together, and the assembly gap between the first limiting protrusions 12 and the second limiting protrusions 31 is 0.5-1.5mm.

[0037] This embodiment, by providing a first limiting ridge 12 and a second limiting ridge 31, allows the culture devices to be stacked, improving the space utilization efficiency of the culture devices. Furthermore, by limiting the assembly gap between the first limiting ridge 12 and the second limiting ridge 31 to 0.5-1.5mm, a good positioning effect is ensured during stacking. This guarantees a tight fit between the devices, reducing unnecessary movement, and provides sufficient tolerance to avoid installation difficulties caused by manufacturing errors. In addition, it effectively prevents tilting or falling during handling or slight shaking, ensuring the safety and stability of the culture process.

[0038] It should be noted that the height of the first limiting protrusion 12 and the second limiting protrusion 31 in this embodiment is 1.5mm.

[0039] like Figure 5 As shown, the liquid-filling plate 1 includes a main body 13 and a handle 14. Two handles 14 are disposed opposite each other on both sides of the main body 13, and the liquid-filling part 11 is disposed on the main body 13. In this embodiment, the main body 13 is 362 mm long and 252 mm wide. By providing handles 14 on both sides of the main body 13, the operator can move the entire culture device more easily and safely.

[0040] It should be noted that the liquid filling plate 1 in this embodiment is made of PC material that conforms to the USP Class VI standard. PC material has good rigidity, is not easy to break, can be sterilized by high temperature and high pressure and frozen, and can be reused, thus reducing the cost of process consumables.

[0041] Furthermore, such as Figure 6 As shown, the liquid-filling plate 1 also includes multiple reinforcing ribs 15. Reinforcing ribs 15 are provided between the main body 13 and the liquid-filling section 11, as well as between two adjacent liquid-filling sections 11, to increase the overall strength of the liquid-filling plate 1. This helps to mitigate external forces such as pressure during handling and localized stress caused by uneven placement. The reinforcing ribs 15 effectively disperse these external forces, preventing damage to the liquid-filling plate 1 due to insufficient load. In this embodiment, the use of reinforcing ribs 15 enhances the mechanical strength of the liquid-filling plate 1, reduces the risk of deformation, and helps extend the service life of the device, ensuring the stability and reliability of the cell culture process.

[0042] See also Figure 7 The main body 13 has a plurality of second protrusions 16 on the side opposite to the cell culture plate 2. The plurality of second protrusions 16 are located at the four corners of the main body 13, and both ends of the second protrusions 16 are provided with inclined surfaces 17.

[0043] In this embodiment, the second boss 16 is configured as the main contact point with the horizontal worktable, rather than allowing the entire bottom surface to directly contact the platform. This effectively reduces wobbling or instability caused by uneven bottom surfaces or minor defects on the worktable surface, ensuring greater stability when the filling plate 1 is placed. Furthermore, by concentrating the contact points at several specific locations (i.e., the four corners), this embodiment better ensures that the filling plate 1 remains horizontal. The beveled surfaces 17 at both ends of the second boss 16 help reduce resistance when removing the finished product from the mold after injection molding, preventing tearing or damage caused by right-angled edges, making the demolding process smoother, reducing the possibility of surface defects, and improving production quality.

[0044] It should be noted that the second protrusions 16 at the four corners of the bottom of the liquid-filling plate 1 are all 1.5mm high.

[0045] like Figure 5 , Figure 8 and Figure 10 As shown, the main body 13 is provided with a first oblique angle structure 18, the plate 21 has a first side 211 and a second oblique angle structure 212, the second oblique angle structure 212 is provided on the first side 211 and is adapted to the first oblique angle structure 18, and the cover plate 3 has a third oblique angle structure 32, which is adapted to the first oblique angle structure 18 and the second oblique angle structure 212.

[0046] This embodiment uses the first oblique angle structure 18, the second oblique angle structure 212 and the third oblique angle structure 32 to give each component (liquid plate 1, cell culture plate 2, cover plate 3) specific shape characteristics, which helps users quickly identify the correct assembly direction and ensure that each component is assembled in the correct direction.

[0047] like Figure 8 and Figure 9 As shown, the plate 21 includes multiple support bars 213 and a support ring 214. Multiple culture sections 22 are disposed within the support ring 214, and the support ring 214 and the culture sections 22 are connected by the support bars 213. The support ring 214 has a first side 211 and a second side 215 disposed opposite to the first side 211. The distance between the support ring 214 near the first side 211 and the culture section 22 is L1, and the distance between the support ring 214 near the second side 215 and the culture section 22 is L2, where L1 = 2L2.

[0048] The support strip 213 in this embodiment not only helps to fix the position of the culture section 22, but also enhances the mechanical strength of the entire cell culture plate 2, preventing the culture section 22 from shifting or being damaged during operation. Furthermore, the different spacings give the cell culture plate 2 a clear orientation, making it easy for users to quickly identify the correct installation direction. For example, the side with the larger spacing should be placed facing outwards towards the liquid-filling plate 1, simplifying the assembly process and reducing the possibility of misoperation.

[0049] Furthermore, the culture section 22 has a third limiting protrusion 24 on the side facing the bottom wall of the liquid-filling section 11, which maintains a certain gap between the bottom of the culture section 22 and the bottom wall of the liquid-filling section 11. Working together with the second venting gap 5, it ensures good gas circulation. By ensuring sufficient space for gas exchange, a better culture environment can be maintained and cell growth can be promoted.

[0050] like Figure 10 and Figure 11 As shown, the cover plate 3 includes a cover body 33, multiple flange structures 34, and a third protrusion 35. The cover body 33 covers the cell culture plate 2. The flange structures 34 are spaced apart on the side of the cover body 33 facing the culture section 22. The flange structures 34 are adapted to the culture section 22 and are arranged one-to-one with the culture section 22, which can effectively prevent the evaporation of culture medium and prevent contamination. The third protrusion 35 is arranged on the side of the cover body 33 facing the culture section 22, so that when the cover plate 3 is placed on the cell culture plate 2, a third air-permeable gap 6 is formed between the cover plate 3 and the plate body 21. The third air-permeable gap 6 is connected to the first air-permeable gap 4, and together they constitute an effective gas exchange system, ensuring sufficient oxygen and carbon dioxide exchange during the culture process and providing the necessary conditions for cell growth.

[0051] This embodiment introduces a flange structure 34 to reduce the evaporation of the culture medium and prevent external contamination; while by setting a third protrusion 35 to form a third venting gap 6 and connecting it with other venting gaps, sufficient gas exchange is ensured, providing a suitable growth environment for cells, guaranteeing gas flow and environmental stability during the culture process, and greatly improving the success rate and efficiency of cell culture.

[0052] It should be noted that the first air gap 4 in this embodiment allows gas to flow out only from the bottom, which reduces the possibility of microorganisms or other contaminants from the outside air entering the culture system. Figure 12 As shown, the 12 culture chambers 221 on the right represent the state of skin cells after about 7 days of culture, while the 12 culture chambers 221 on the left represent the state of skin cells after about 15 days of culture. Skin cells cultured for about 15 days can be transplanted into the human body for propagation.

[0053] In summary, this utility model provides a culture device that solves the problem of excessive flow rate during rapid fluid replenishment by setting multiple liquid inlet channels 222 in the culture chamber 221, and ensures sufficient gas exchange by setting a double-layer air-permeable gap, thus overcoming the problem that traditional culture tools cannot meet the rapid fluid replenishment requirements of artificial skin cell culture.

[0054] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and substitutions can be made without departing from the technical principles of the present utility model, and these improvements and substitutions should also be considered within the protection scope of the present utility model.

Claims

1. A culture device, characterized by, The cell culture plate comprises a liquid loading plate, a cell culture plate, and a cover plate. The cell culture plate is installed on the liquid loading plate. The cover plate is arranged on the cell culture plate and the liquid loading plate. A first air permeable gap is formed between the cover plate and the liquid loading plate. The cell culture plate comprises a plate body and a plurality of culture parts arranged at intervals on the plate body. The liquid loading plate has a liquid loading part for placing the culture part. The culture part has a culture cavity and a plurality of liquid inlet channels. The plurality of liquid inlet channels are arranged on the groove wall of the culture cavity to connect the culture cavity and the liquid loading part.

2. The culture device of claim 1, wherein A plurality of first bosses are arranged on the side of the plate body facing the liquid loading plate. The plurality of first bosses are arranged at intervals around the axis of the corresponding culture part on the side of the culture part. When the culture part is installed on the liquid loading part, a second air permeable gap is formed between the plate body and the liquid loading plate. The second air permeable gap is connected with the first air permeable gap.

3. The culture device of claim 1, wherein The liquid inlet channel comprises a liquid inlet hole and a liquid inlet groove. A plurality of liquid inlet holes are arranged at intervals on the bottom wall of the culture cavity around the axis of the culture cavity. A plurality of liquid inlet grooves are arranged at intervals on the side wall of the culture cavity.

4. The culture device of claim 1, wherein A plurality of first limiting ribs are arranged on the side of the liquid loading part away from the cell culture plate. A plurality of second limiting ribs are arranged on the side of the cover plate away from the cell culture plate. The first limiting ribs are matched with the second limiting ribs. The assembly gap between the first limiting ribs and the second limiting ribs is 0.5-1.5 mm.

5. The culture device of claim 1, wherein The liquid loading plate comprises a main body part and a handle part. Two handle parts are arranged on the two sides of the main body part. The liquid loading part is arranged on the main body part. The liquid loading plate further comprises a plurality of reinforcing ribs. The reinforcing ribs are arranged between the main body part and the liquid loading part and between adjacent liquid loading parts.

6. The culture device of claim 5, wherein A plurality of second bosses are arranged on the side of the main body part away from the cell culture plate. The plurality of second bosses are arranged on the four corner parts of the main body part. The two ends of the second boss are provided with inclined surfaces.

7. The culture device of claim 5, wherein The main body part is provided with a first bevel structure. The plate body has a first side and a second bevel structure. The second bevel structure is arranged on the first side and matched with the first bevel structure. The cover plate has a third bevel structure. The third bevel structure is matched with the first bevel structure and the second bevel structure.

8. The culture device of claim 7, wherein The plate body comprises a plurality of support strips and a support ring. A plurality of culture parts are arranged in the support ring. The support ring and the culture part are connected by the support strips. The support ring has a first side and a second side arranged opposite to the first side. The distance between the support ring close to the first side and the culture part is L1. The distance between the support ring close to the second side and the culture part is L2. L1=2L2.

9. The culture apparatus according to claim 1, characterized by The culture part has a third limiting rib on the side facing the bottom wall of the liquid loading part.

10. The culture apparatus according to claim 1, characterized by The cover plate comprises a cover body, a plurality of flange structures and a third boss, the flange structures are arranged on the side of the cover body facing the culture part, the flange structures are matched with the culture part, and the flange structures are arranged one by one corresponding to the culture part, and the third boss is installed on the side of the cover body facing the culture part, so that when the cover plate is arranged on the cell culture plate, a third air permeable gap is formed between the cover plate and the plate body, and the third air permeable gap is communicated with the first air permeable gap.

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